James Callaway, an Artist with a Drill and a Brush.

Dr. James A. Callaway (1947-2022) (Fig.1) was a well-known dentist and Renaissance-Man for over 50 years in Las Vegas, NV. Callaway attended the University of Nevada Reno as an undergraduate then matriculated into the University of Detroit Mercy School of Dentistry where he earned a D.D.S. Summa Cum Laude. He then moved back to his hometown of Las Vegas to begin practice.

Quantification of damage to eelgrass (Zostera marina) beds and evidence-based management strategies for boats anchoring in San Francisco Bay.

Seagrasses are highly productive, but human nearshore activities have reduced their global distribution by >29% since the twentieth century. In the United States and Canada, the native seagrass Zostera marina (eelgrass) provides habitat for many species and multiple ecosystem services. By supplying spawning surface for fish and substrate for invertebrates, eelgrass creates foraging areas for high densities of migratory birds. Eelgrass beds stabilize sediment, protect adjacent shorelines, improve water quality, and sequester carbon in their underlying substrate. San Francisco Bay (California, USA) is a significant estuary for eelgrass, and recent surveys show that eelgrass beds are in decline. Protecting eelgrass is a conservation priority for federal, state, and local agencies, yet few studies have documented the extent of eelgrass loss due to human impacts such as boat anchoring. The purpose of our study was to provide factual evidence for policy makers by quantifying damage to eelgrass caused by illegal anchor-outs in San Francisco Bay, an issue that has been disputed for decades. Using aerial imagery and GIS analyses, we determined the amount of direct damage to eelgrass caused by anchor-outs. We found that boats damage up to 41% of the eelgrass bed, and each boat may cause up to 0.3 ha of damage. These results can be used to inform decisions about anchor-outs by stakeholders and government agencies. Furthermore, our efficient analytical approach could be implemented in other coastal regions.

Root Tip Recovery via Root Canal Instrumentation.

This article reviews 2 representative cases of root tip recovery by root canal instrumentation. Recommendations associated with the use of root canal instrumentation for root tip recovery within mandibular and maxillary alveoli are considered.

Pulseless Ventricular Tachycardia During Office-Based Anesthetic in a Four-Year-Old Child.

A 4-year-old 16-kg boy presented for full mouth dental rehabilitation in a private pediatric dental office. The patient had no significant previous medical history. Upon sevoflurane induction by a dentist anesthesiologist, the patient converted from normal sinus rhythm to pulseless ventricular tachycardia. Advanced cardiac life support protocol was initiated. After 2 automatic external defibrillator shocks were delivered in conjunction with epinephrine administration, the patient returned to normal sinus rhythm. The patient was transported via emergency medical service paramedics to a local children's hospital emergency room where he was observed uneventfully for 24 hours prior to discharge.

VIZR--an automated chemometric technique for metabolic profiling.

A chemometric technique, visual interpretation of z-score ratios (VIZR), written in the open source code R, has been developed to identify metabolic differences between individual biosamples and a control group. To demonstrate the capabilities of VIZR, 49 urine samples were collected from healthy volunteers: 41 samples were collected randomly following a normal dietary routine and 7 test samples were collected after dietary supplementation with either ibuprofen or alcoholic beverages. An eighth test sample was prepared by 50% dilution of a control sample. Sample analysis was conducted by (1)H nuclear magnetic resonance (NMR) spectroscopy and the collected data were subjected to VIZR analysis, which successfully discriminated each of the 8 test samples from the 41 control samples. In addition, VIZR analysis revealed the NMR spectral regions responsible for the disparity between the individual test samples and the control group. The self-normalizing nature of the VIZR calculation provides a robust analysis independent of dilution effects, which is especially important in urine analyses. Potential applications of VIZR include high-throughput data analysis for toxicological profiling, disease diagnosis, and biomarker identification in any type of biosample for which a control dataset can be established. Although demonstrated herein for the statistical analysis of (1)H NMR data, the VIZR program is platform independent and could be applied to digitized metabolic datasets acquired using other techniques including hyphenated mass spectrometry measurements.

Office accreditation experiences with 3 accrediting agencies and suggestions for changes in private oral and maxillofacial surgery facility evaluations.

Historically, oral and maxillofacial surgeons have had considerable autonomy in operating their offices. Oral and maxillofacial surgeons have had a singular history of safety, training, and success in outpatient anesthesia in their offices. However, preventable patient morbidity and mortality in private office-based surgical facilities of a variety of professions have brought increased scrutiny to the office environment. The present report describes the experiences of 3 oral and maxillofacial surgeons with 3 accrediting agencies in obtaining office accreditation and offers recommendations to be considered for the future of our specialty in terms of private office certification.

Student-to-student local anesthesia injections in dental education: moral, ethical, and legal issues.

This article reports the findings of a survey-based study conducted to determine U.S. dental schools' institutional protocols regarding the practice of students' administering local anesthetic injections to fellow students as part of their process of learning this skill. The majority of schools ask students to practice local anesthetic injections on each other without obtaining informed consent.

Extracts from benthic anatoxin-producing Phormidium are toxic to 3 macroinvertebrate taxa at environmentally relevant concentrations.

Toxin-producing cyanobacteria are increasing in rivers and streams globally, leading to growing concerns over their potential impacts on aquatic ecosystems. The present study was designed to culture field-collected Phormidium in the laboratory, identify individual species, conduct chemical analyses to identify cyanotoxins, and conduct toxicity tests to investigate the potential for this genera to impact stream health. Freshwater toxicity tests were conducted with standard US Environmental Protection Agency invertebrate test protocols with culture water used to grow 3 Phormidium strains isolated from the Russian River (CA, USA). Enzyme linked immunosorbent assays were used to measure total anatoxin concentrations. Culture waters from the 3 Phormidium strains were highly toxic to Ceriodaphnia dubia, Hyalella azteca, and Chironomus dilutus. The C. dubia 7-d survival median lethal concentrations were 0.71, 0.49, and 0.56 µg/L anatoxin for Phormidum strains 1, 2, and 3, respectively. The 7-d reproduction inhibitory concentrations, 25% were 0.55, 0.32, and 0.30 µg/L anatoxin for strains 1, 2, and 3, respectively. Chironomus dilutus survival was reduced at concentrations <2 µg/L anatoxin by all 3 strains, and the H. azteca 96-h lethal concentrations, 25% were 2.82, 1.26, and 5.30 µg/L for strains 1, 2, and 3, respectively. Additional liquid chromatography-mass spectrometry analyses demonstrated that the likely anatoxin variant in these cultures was dihydro-anatoxin-a. The results suggest that anatoxins produced by Phormidium have the potential to impact stream macroinvertebrates. Environ Toxicol Chem 2018;37:2851-2859. © 2018 SETAC.

Obtaining written informed consent for the administration of local anesthetic in dentistry.

BACKGROUND: The purpose of this study was to examine the frequency with which dentists obtain written informed consent for the administration of local anesthetic in dentistry. METHODS: The authors administered an informal survey to 252 dentists. RESULTS: Most respondents who practiced a dental specialty or limited their practice to anesthesiology for dentistry obtained written informed consent for the administration of local anesthetic. Most general practitioners did not. CONCLUSIONS: Written informed consent appears to be obtained more often by dental specialists and dentists limiting their practices to anesthesiology for dentistry than by general practitioners. PRACTICE IMPLICATIONS: All dentists may want to consider obtaining written informed consent for the administration of local anesthetic.

1H NMR-based metabolomics methods for chemical genomics experiments.

Metabolomics and chemical genomics studies can each provide unique insights into plant biology. Although a variety of analytical techniques can be used for the interrogation of plant systems, nuclear magnetic resonance (NMR) provides unbiased characterization of abundant metabolites. An example methodology is provided for probing the metabolism of Arabidopsis thaliana in a chemical genomics experiment including methods for tissue treatment, tissue collection, metabolite extraction, and methods to minimize variance in biological and technical sample replicates. Additionally, considerations and methods for data analysis, including multivariate statistics, univariate statistics, and data interpretation are included. The process is illustrated by examining the metabolic effects of chemical treatment of Arabidopsis with Sortin 1, also known as vacuolar protein sorting inhibitor 1. Sortin 1 was applied to Arabidopsis seedlings to examine metabolic effects in a chemical genomics experiment and to demonstrate the utility of metabolomics in conjunction with other "omics" techniques.