RESUMO
AIM: To evaluate the technical application of fluorescence in situ hybridization (FISH) as a support to classical cytogenetic in numerical chromosomal aneuploidies studies in samples of amniotic fluid, chorionic villus, and fetal loss. MATERIALS AND METHODS: The authois performed cytogenetic analyses in 1,409 patients (678 amniocentesis, 512 chorionic villus samples, and 219 spontaneous abortions) during one year. FISH molecular study aided traditional cytogenetic in 90 cases. These cases were indicated based on the diagnostic hypothesis of each patient or when no cellular growth was obtained. The authors standardized the FISH in discoloured slides. RESULTS: They had 85% positive FISH in amniotic fluid, 70% in chorionic villus, and 90% in abortion material using 13, 18, 21 X and Y centromeric probes. It showed 12% of altered FISH in amniotic fluid (100% trisomies), 10% in chorionic'villus (50% trisomy and 50% X - monosomy), and 22% in abortion material (50% trisomy, 25% X-monosomy, and 25% triploidy). FISH and cytogenetic analysis confirmed the results. CONCLUSION: This technique revolutionized clinical and research applications of cytogenetics. In this particular paper, FISH was a valuable and reliable technique to promptly identify rapid detection of aneuploidies in interphase cells, metaphase spread and paraffin-embedded samples. It is hoped that, in the future, the economic viability of array CGH and FISH, with the decreasing cost of testing and their genomics advantages can be incorporated as routine and customized in the approach of prenatal diagnosis.
Assuntos
Aneuploidia , Análise Citogenética/métodos , Hibridização in Situ Fluorescente/métodos , Diagnóstico Pré-Natal/métodos , Adulto , Feminino , Humanos , Masculino , Pessoa de Meia-Idade , GravidezRESUMO
This study characterized the structure and the morphocytochemical, immunohistochemical and ultrastructural aspects of the head kidney (HK) of the fat snook Centropomus parallelus. The HK is enclosed by a thin capsule of connective tissue, from which fine trabeculae originate and branch into the interior of organ. In the parenchyma, there are aggregates of lymphoid cells containing populations of lymphocytes T immunopositive for CDRO45, in a nodular arrangement, around blood vessels and melano-macrophage centres. Among the cells that constituted these aggregates and surrounded them, were macrophages and monocytes, and their precursors, with strong immunopositivity for CD68, along with cells of the granulocytic lineage in various phases of maturation positive for lysozyme and PAS. Macrophages and chromaffin and interrenal cells are also present. Ultrastructurally, the HK comprises a reticulum-endothelial stroma consisting of endothelial cells, reticulocytes of the fibroblast type and macrophage type and a parenchyma with increased cellularity, principally blood cells of the erythrocytic, granulocytic, lymphocytic, monocytic and thrombocytic series.
Assuntos
Rim Cefálico/ultraestrutura , Perciformes/anatomia & histologia , Animais , Rim Cefálico/citologia , Rim Cefálico/imunologia , Linfócitos/citologia , Linfócitos/ultraestrutura , Microscopia Eletrônica de Transmissão , Linfócitos T/citologia , Linfócitos T/ultraestruturaRESUMO
Ulcerative colitis (UC) is a disease of the colon and rectum characterized by a nonspecific chronic inflammation mediated by the concerted response of cellular and humoral events. Prostaglandins are synthesized by cyclooxygenase (COX)-1 and -2 and exhibit both pro- and anti-inflammatory activity. To evaluate COX-1 and COX-2 immunoexpression in 42 cases of UC and to correlate it with clinicopathological parameters, COX-1 and COX-2 expression was investigated by the immunohistochemistry method. Only patients with all pertinent clinical and evolutive data as well as with adequate biopsy material were included in the study. Fifteen samples of colorectal adenocarcinoma and 14 of large bowel with no histological changes were used for positive and negative controls, respectively. UC patients showed COX-1 immunoreactivity in epithelial cells in 29% of the cases and in inflammatory cells in 43%. COX-2 positivity in epithelial and inflammatory cells was found in 69% of the samples. The comparison between UC and the control groups revealed that the UC group had significantly more positive cases for COX-1 and COX-2 in inflammatory cells. Immunohistochemistry allowed the identification of COX-1 and COX-2 expression in epithelial and inflammatory cells in UC biopsies. No significant difference between COX-1 and COX-2 immunoreactivity in epithelial and inflammatory cells was observed regarding the clinicopathological parameters. COX-2 presented low expression in normal colon and high expression in colorectal adenocarcinoma. COX-2 might play a role in the pathophysiologic processes of inflammatory bowel disease and the development of neoplasia. Treatment with selective COX-2 inhibitors might be an additional option for therapy.
Assuntos
Colite Ulcerativa/enzimologia , Neoplasias Colorretais/enzimologia , Ciclo-Oxigenase 1/metabolismo , Ciclo-Oxigenase 2/metabolismo , Adolescente , Adulto , Idoso , Colite Ulcerativa/patologia , Neoplasias Colorretais/patologia , Feminino , Seguimentos , Humanos , Imuno-Histoquímica , Masculino , Pessoa de Meia-Idade , Índice de Gravidade de DoençaRESUMO
The aim of this study was to investigate if grape juice concentrate is able to protect rat liver against cadmium toxicity. For this purpose, histopathological analysis, cytochrome C expression and immunoexpresssion of metalloproteinases (MMP) 2 and 9 were investigated. A total of 15 Wistar rats weighing 250 g on the average, and 8 weeks age were distributed into 3 groups (n=5), as follows: Control group (non-treated group, CTRL); Cadmium group (Cd) and grape juice concentrate group (Cd+GJ). Histopathological analysis revealed that liver from animals treated with grape juice concentrate improved tissue degeneration induced by cadmium intoxication. Animals intoxicated with cadmium and treated with grape juice concentrate showed higher cytochrome C gene expression in liver cells. No significant statistically differences (p>0.05) were found to MMP 2 and 9 immunoexpression between groups. Taken together, our results demonstrate that grape juice concentrate is able to prevent tissue degeneration in rat liver as a result of increasing apoptosis.
Assuntos
Intoxicação por Cádmio/prevenção & controle , Citocromos c/biossíntese , Fígado/efeitos dos fármacos , Metaloproteinase 2 da Matriz/biossíntese , Metaloproteinase 9 da Matriz/biossíntese , Extratos Vegetais/farmacologia , Vitis/química , Animais , Intoxicação por Cádmio/enzimologia , Intoxicação por Cádmio/patologia , Sucos de Frutas e Vegetais , Fígado/enzimologia , Fígado/patologia , Masculino , Necrose/enzimologia , Necrose/patologia , Necrose/prevenção & controle , Substâncias Protetoras/farmacologia , RatosRESUMO
The growth of tumors is highly variable and this probably reflects even its clinical course. The monoclonal antibody Ki-67 recognises an antigen present in the nuclei of cells in all phases of the cell cycle except G0. In the current study, we examined by immunohistochemistry the proliferative activity, based on Ki-67 labeling index (Ki67LI), in formalin-fixed and paraffin-embedded sections of 152 tumors, being 70 gastric and 89 colorectal cancers. The results obtained were correlated with the clinicopathologic factors. The carcinomas showed a wide range of Ki-67LI, reflecting a variation in proliferative activity. The tumor labeling index ranged from 10 to 85 per cent positivity, being the mean level in gastric cancer tissue 0.52 and in colorectal cancer 0.44. There was also heterogeneity of labeling within many of the tumors. No significant correlation was found between Ki-67LI and sex, age, clinical stage in these cancers. In colorectal cancer, but not in gastric cancer, high levels of Ki67LI have been correlated with poor survival. Ki-67 staining is a simple and useful method for estimating proliferative activity. The importance of Ki-67 as an indicator of tumor behaviour is not clear. In colorectal cancer this index may be used as a marker of prognosis.
Assuntos
Biomarcadores Tumorais/análise , Neoplasias Colorretais/metabolismo , Neoplasias Colorretais/patologia , Antígeno Ki-67/metabolismo , Neoplasias Gástricas/metabolismo , Neoplasias Gástricas/patologia , Proliferação de Células , Neoplasias Colorretais/mortalidade , Feminino , Humanos , Imuno-Histoquímica , Masculino , Pessoa de Meia-Idade , Prognóstico , Estudos Retrospectivos , Neoplasias Gástricas/mortalidade , Análise de SobrevidaRESUMO
The purpose of the present study was to evaluate breast carcinoma samples before and two days after treatment with tamoxifen in order to analyse early histopathological alterations--particularlynuclear alterations-- as well as immunohistochemical expression of Ki-67, Erb-B2, VEGF, TGF-beta1 and ILK proteins. Twenty one cases of invasive ductal and lobular breast carcinoma were studied. Patients were submitted to biopsy of the lesion and, after confirmation of the diagnosis, they received 20 mg of tamoxifen a day, beginning two days before surgery. The samples obtained during biopsy and after surgery were stained with HE for histopathological diagnosis. Estrogen receptor was positive in 18 cases and negative in 3. The immunohistochemical method was applied for the detection of Ki-67, Erb-B2, protein, vascular endothelial growth factor (VEGF), transforming growth factor beta (TGF-beta1) and integrin linked kinase (ILK). Two days after tamoxifen treatment, the following results were observed: 1) decrease in the cell volume, chomatine condensation, nucleoli less evident and clearly defined nuclear limits; 2) significant reduction in the expression of Erb-B2 protein and significant increase in the expression of TGF-beta1 protein; 3) expression of others proteins (Ki-67, VEGF and ILK) was not altered during the indicated time frame. Our results suggest that analyzing nuclear alterations and expression of Erb-B2 and TGF-beta1 proteins would be useful to assess the initial response to tamoxifen.
Assuntos
Neoplasias da Mama/metabolismo , Núcleo Celular/patologia , Antígeno Ki-67/metabolismo , Proteínas Serina-Treonina Quinases/metabolismo , Receptor ErbB-2/metabolismo , Tamoxifeno/uso terapêutico , Fator de Crescimento Transformador beta/metabolismo , Fatores de Crescimento do Endotélio Vascular/metabolismo , Neoplasias da Mama/ultraestrutura , Carcinoma Ductal de Mama/tratamento farmacológico , Carcinoma Ductal de Mama/metabolismo , Carcinoma Lobular/tratamento farmacológico , Carcinoma Lobular/metabolismo , Humanos , Imuno-Histoquímica , Fator de Crescimento Transformador beta1RESUMO
The objective was to study the morphology of the articular disc and analyse the immunohistochemical expression of types I and III collagen markers in the temporomandibular joint (TMJ) disc of human foetuses of different gestational ages. Twenty TMJ from human foetuses supplied by Universidade Federal de Uberaba with gestational ages from 17 to 24 weeks were studied. The gestational age of the foetuses was determined by measuring the crown-rump (CR) length. Macroscopically, the foetuses were fixed in 10% formalin solution and dissected by removing the skin and subcutaneous tissue and exposing the deep structures. Immunohistochemical markers of type I and III were used to characterize the existence of collagen fibres. Analysis of the immunohistochemical markers of types I and III collagen revealed the presence of heterotypical fibril networks.
Assuntos
Colágeno Tipo II/biossíntese , Colágeno Tipo I/biossíntese , Feto/embriologia , Regulação da Expressão Gênica no Desenvolvimento/fisiologia , Disco da Articulação Temporomandibular/embriologia , Feminino , Feto/citologia , Idade Gestacional , Humanos , Imuno-Histoquímica , Masculino , Disco da Articulação Temporomandibular/citologiaRESUMO
OBJECTIVES: The aim of the present study was to evaluate DNA damage (micronucleus) and cellular death (pyknosis, karyolysis and karyorrhexis) in exfoliated buccal mucosa cells from adults following cone beam CT exposure. METHODS: A total of 19 healthy adults (10 men and 9 women) submitted to cone beam CT were included. RESULTS: No significant statistically differences (P > 0.05) in micronucleus frequency were seen before and after cone beam CT exposure. In contrast, the tomography was able to increase other nuclear alterations closely related to cytotoxicity such as karyorrhexis, pyknosis and karyolysis (P < 0.05). CONCLUSION: In summary, these data indicate that cone beam CT may not be a factor that induces chromosomal damage, but it is able to promote cytotoxicity.
Assuntos
Tomografia Computadorizada de Feixe Cônico , Dano ao DNA , Mucosa Bucal/efeitos da radiação , Adulto , Morte Celular/efeitos da radiação , Núcleo Celular/efeitos da radiação , Cromatina/efeitos da radiação , Tomografia Computadorizada de Feixe Cônico/efeitos adversos , Feminino , Humanos , Masculino , Micronúcleos com Defeito Cromossômico/efeitos da radiação , Mucosa Bucal/citologiaRESUMO
The aim of this study was to evaluate the rat ovarian morphological and function changes after pinealectomy (px). Two months after px, young female Wistar rats were sacrificed and the right ovaries were analysed morphologically and the left ovaries were used for steroid receptor binding experiments. Blood was collected and steroid hormone and melatonin levels were measured using radioimmunoassay kits. Results revealed that in the px group the rat ovaries had an increase in the number of atretic follicles and interstitial cells. These cells showed hyperactivity features on transmission electron microscopy and morphometric analysis (p < 0.05 compared with control and sham groups). Px-group serum showed an increase in estradiol (p < 0.05) and a decrease in progesterone levels (p < 0.05) compared with other groups. Moreover, progesterone receptor expression was lower than control and sham groups (p < 0.05). We postulate that pinealectomy leads to many morphological alterations of rat ovaries that are associated with functional changes in steroidogenesis and a decrease in progesterone receptor expression.
Assuntos
Ovário/metabolismo , Ovário/ultraestrutura , Glândula Pineal/cirurgia , Receptores de Progesterona/metabolismo , Células Tecais/ultraestrutura , Animais , Contagem de Células , Retículo Endoplasmático Rugoso/ultraestrutura , Retículo Endoplasmático Liso/ultraestrutura , Estradiol/sangue , Estradiol/metabolismo , Feminino , Complexo de Golgi/ultraestrutura , Células da Granulosa , Contagem de Leucócitos , Melatonina/sangue , Microscopia Eletrônica , Mitocôndrias/ultraestrutura , Glândula Pineal/fisiologia , Pregnenodionas/metabolismo , Progesterona/sangue , Ratos , Ratos Wistar , Receptores de Estrogênio/metabolismo , Testosterona/sangue , TrítioRESUMO
Ulcerative colitis (UC) is a disease of the colon and rectum characterized by a nonspecific chronic inflammation mediated by the concerted response of cellular and humoral events. Prostaglandins are synthesized by cyclooxygenase (COX)-1 and -2 and exhibit both pro- and anti-inflammatory activity. To evaluate COX-1 and COX-2 immunoexpression in 42 cases of UC and to correlate it with clinicopathological parameters, COX-1 and COX-2 expression was investigated by the immunohistochemistry method. Only patients with all pertinent clinical and evolutive data as well as with adequate biopsy material were included in the study. Fifteen samples of colorectal adenocarcinoma and 14 of large bowel with no histological changes were used for positive and negative controls, respectively. UC patients showed COX-1 immunoreactivity in epithelial cells in 29 percent of the cases and in inflammatory cells in 43 percent. COX-2 positivity in epithelial and inflammatory cells was found in 69 percent of the samples. The comparison between UC and the control groups revealed that the UC group had significantly more positive cases for COX-1 and COX-2 in inflammatory cells. Immunohistochemistry allowed the identification of COX-1 and COX-2 expression in epithelial and inflammatory cells in UC biopsies. No significant difference between COX-1 and COX-2 immunoreactivity in epithelial and inflammatory cells was observed regarding the clinicopathological parameters. COX-2 presented low expression in normal colon and high expression in colorectal adenocarcinoma. COX-2 might play a role in the pathophysiologic processes of inflammatory bowel disease and the development of neoplasia. Treatment with selective COX-2 inhibitors might be an additional option for therapy.
Assuntos
Adolescente , Adulto , Idoso , Feminino , Humanos , Masculino , Pessoa de Meia-Idade , Colite Ulcerativa/enzimologia , Neoplasias Colorretais/enzimologia , Ciclo-Oxigenase 1/metabolismo , /metabolismo , Colite Ulcerativa/patologia , Neoplasias Colorretais/patologia , Seguimentos , Imuno-Histoquímica , Índice de Gravidade de DoençaRESUMO
O objetivo deste trabalho foi a determinaçäo das concentraçöes dos receptores de estrógeno (ER) e progesterona (PR) em exocérvix humana. Ensaiamos ER e PR em vários "pools" de 25 fragmentos de exocérvix de mulheres normais obtidos por curetagem e em endométrios obtidos de pacientes submetidas à histerectomia por condiçöes näo malignas. Os "pools" de fragmentos de exocérvix foram separados em quatro tipos: 1) os referentes às mulheres na fase luteal; 2) na fase proliferativa do ciclo menstrual; 3) em uso de anticoncepcionais e 4) na menopausa. As dosagens dos receptores de estrógeno e progesterona foram feitas pelo método de carväo-dextrana. As concentraçöes de ER e PR encontradas na exocérvix foram respectivamente 15% e 3,6% das encontradas no endométrio, mas com constantes de afinidade semelhantes. Näo detectamos flutuaçöes na concentraçäo de ER e PR em exocérvix durante a fase proliferativa e luteal. Nas amostras de exocérvix de usuárias de anticoncepcionais, apenas o nível de PR foi estatisticamente menor quando comparado aos níveis obtidos na fase luteal e proliferativa. Os valores de ER e PR em exocérvix de mulheres pós-menopáusicas säo estatisticamente maiores do que os encontrados em mulheres pré-menopáusicas. Näo há diferenças em relaçäo ao tempo de menopausa. O efeito de estrógeno e progesterona na porçäo cervical é aparentemente limitado pela baixa concentraçäo dos seus respectivos receptores