Mitochondrial 16S rRNA gene analysis reveals occurrence of Rhipicephalus sanguineus sensu stricto from steppe and high plateaus regions, Algeria.

The present study aims to perform comparative genetic characterization for R. sanguineus s.l. infesting Algerian dogs living in steppe and high plateau regions based on mitochondrial 16S rRNA gene sequencing and phylogenetic analysis. In total, 1043 ticks were collected from 147 infested dogs, including 756 ticks from 124 dogs in Djelfa and 287 ticks from 23 dogs in Bordj-Bou-Arreridj. All ticks were morphologically identified as R. sanguineus s.l., and 87 specimens were randomly selected for molecular analysis. Sequences obtained from selected ticks clustered with sequences of R. sanguineus s.l. from France, Germany, Portugal, and Spain, all of which belong to R. sanguineus s.s. The occurrence of R. sanguineus s.s. in Algeria is an interesting result given that most R. sanguineus in Africa cluster among the tropical lineage. This finding should prompt further investigation by exhaustive studies across Algerian territory. Such data can be useful to understand the dispersal route of R. sanguineus s.s. from Europe to North Africa, or whether their occurrence in Algeria is a result of historical biogeography.

The Red Fox (Vulpes vulpes) as Sentinel for Tick-Borne Encephalitis Virus in Endemic and Non-Endemic Areas.

Tick-borne encephalitis (TBE) is one of the most important viral zoonosis caused by a neurotropic arbovirus (TBEV). In Germany, TBE is classified as a notifiable disease with an average of 350 autochthonous human cases annually. The incidence-based risk assessment in Germany came under criticism because every year, a number of autochthonous human TBE cases have been detected outside of the official risk areas. Therefore, it is necessary to find additional parameters to strengthen TBEV surveillance. The aim of this study was to examine red foxes as sentinels for TBE. Thus far, there are no published data about the sensitivity and specificity for serological methods testing fox samples. Hence, we aimed to define a system for the screening of TBEV-specific antibodies in red foxes. A total of 1233 fox sera were collected and examined by ELISA and IIFA and confirmed by micro-NT. The overall seroprevalence of antibodies against TBEV in red foxes from Germany confirmed by micro-NT was 21.1%. The seroprevalence differed significantly between risk (30.5%) and non-risk areas (13.1%), with good correlations to local TBE incidence in humans. In conclusion, serological monitoring of red foxes represents a promising surrogate marker system and may even determine unexpected TBEV foci in regions currently regarded as non-risk areas.