RESUMO
An 82-year-old woman with nephrotic syndrome and a 61-year-old woman with proteinuria and purpura on the lower extremities are reported. Both patients had test results positive for hepatitis C virus (HCV) antibody, but HCV RNA was not detected in the blood of either patient. The kidney biopsy showed membranoproliferative glomerulonephritis with capillary deposition of C3 and immunoglobulin M, indicating HCV-associated glomerulonephritis. These cases are suggestive to study the pathogenesis of this disease.
Assuntos
Glomerulonefrite/etiologia , Hepacivirus/imunologia , Hepatite C/complicações , Doenças do Complexo Imune/etiologia , Glomérulos Renais/patologia , RNA Viral/sangue , Adulto , Idoso , Idoso de 80 Anos ou mais , Carcinoma de Células Escamosas/cirurgia , Complemento C3/análise , Neoplasias Faciais/cirurgia , Evolução Fatal , Feminino , Glomerulonefrite/imunologia , Insuficiência Cardíaca/etiologia , Hepatite C/imunologia , Anticorpos Anti-Hepatite C/sangue , Humanos , Imunoglobulina G/imunologia , Imunoglobulina M/análise , Glomérulos Renais/química , Glomérulos Renais/imunologia , Síndrome Nefrótica/etiologia , Complicações Pós-Operatórias/imunologia , Reação em Cadeia da Polimerase Via Transcriptase Reversa , Fator Reumatoide/análise , Fator Reumatoide/imunologiaRESUMO
We present a case of hepatoid carcinoma of the abdominal skin in a male Wistar rat. Histopathologically, this carcinoma resembled human hepatocellular carcinoma with respect to trabecular-sinusoidal structures. Carcinoma tissues contain numerous eosinophilic globules and crystals, and in this case, we found the characteristic eosinophilic globules in the hepatoid carcinoma cells and the crystals in the extracellular portions. Vivid carcinoma cells full of eosinophilic globules were present near the necrotic areas in tumor tissue, wherein quadrate crystals unstained with eosin were observed. PAS staining after diastase digestion revealed that the globules were PAS positive and diastase resistant. In addition, we found that the hepatoid carcinoma cells were immunoreactive for alpha-1-antitrypsin (anti-A1AT) antibody with the globules and crystals staining peripherally, and a central unstained region. Ultrastructural study of intracytoplasmic globules and extracellular crystals revealed that the fringe of each globule and crystal had no limiting membrane and showed the same level of electron density. These findings suggest that the characteristic crystals in this tumor may have originated from the globules that were emitted from the carcinoma cells after their death as a result of saturation with intracytoplasmic globules.