Physio-biochemical characterization of wheat genotypes under temperature stress.

Thermal stress is a major abiotic stress in wheat and is highly complex in mechanism. A large area in northwestern plain zones (NWPZ), which is the wheat bowl of India is affected by heat stress. Climate change also causes an abrupt increase in temperature at different growth stages of wheat. Thus, wiser selection of stress tolerant varieties is an important strategy to combat the climate change effect. The present study aims for physiological and biochemical screening of timely sown NWPZ wheat varieties (WB2, HD3086, DBW88, DPW621-50, DBW17, HD2967 and PBW550) of India for their thermal stress tolerance along with heat tolerant (RAJ3765) and susceptible checks (RAJ4014) at seedling stage. The experiment was conducted in completely randomized design under controlled laboratory condition and heat stress was induced at 37 °C at seedling stage. Later different physio-biochemical traits were studied in both control and stress seedlings. All traits exhibited significant variations among genotypes under heat stress condition. Root and shoot weight, relative water content, chlorophyll content index and chlorophyll fluorescence reduced significantly, whereas membrane leakage, osmotic potential, catalase, ascorbate peroxidase, guaiacol peroxidase, malondialdehyde content and proline content were increased in stress plants. A tolerance matrix was prepared based on stress response of the genotypes for each trait and a final tolerance score was given to each genotype. Based on this tolerance matrix, DBW88 and PBW550 were identified as tolerant, DPW621-50, DBW17 and HD2967 as moderately susceptible and HD3086 and WB2 as susceptible to heat stress. Earlier studies parade that seedling level stress tolerance has high correlation with adult level stress tolerance under field condition in wheat. Hence, this study helps in wiser selection of varieties for sowing in NWPZ based on weather forecast of the location for creating varietal mosaic in context of climate change.

Physiological traits associated with heat tolerance in bread wheat (Triticum aestivum L.).

Field experiments for evaluating heat tolerance-related physiological traits were conducted for two consecutive years using a mapping population of recombinant inbred lines (RILs) from the cross RAJ4014/WH730. Chlorophyll content (Chl) and chlorophyll fluorescence (CFL) were recorded under timely sown (TS) and late sown (LS) conditions. Late sowing exposes the terminal stage of plants to high temperature stress. Pooled analysis showed that CFL and Chl differed significantly under TS and LS conditions. The mean value of CFL (Fv/Fm) and Chl under both timely and late sown conditions were used as physiological traits for association with markers. Regression analysis revealed significant association of microsatellite markers viz., Xpsp3094 and Xgwm131 with coefficients of determination (R (2)) values for CFL (Fv/Fm) and Chl as 12 and 8 %, respectively. The correlation between thousand grain weight (TGW) with Chl and CFL were 14 and 7 % and correlation between grain wt./spike with Chl and CFL were 15 and 8 %, respectively. The genotypes showing tolerance to terminal heat stress as manifested by low heat susceptibility index (HSI = 0.43) for thousand grain weight, were also found having very low Chl, HSI (-0.52). These results suggest that these physiological traits may be used as a secondary character for screening heat-tolerant genotypes.

Image facilitated assessment of intra-spike variation in grain size in wheat under high temperature and drought stress.

In wheat (Triticum aestivum L.), the grain size varies according to position within the spike. Exposure to drought and high temperature stress during grain development in wheat reduces grain size, and this reduction also varies across the length of the spike. We developed the phenomics approach involving image-based tools to assess the intra-spike variation in grain size. The grains were arranged corresponding to the spikelet position and the camera of smart phone was used to acquire 333 images. The open-source software ImageJ was used to analyze features of each grain and the image-derived parameters were used to calculate intra-spike variation as standard deviation (ISVAD). The effect of genotype and environment were highly significant on the ISVAD of grain area. Sunstar and Raj 4079 contrasted in the ISVAD of grain area under late sown environment, and RNA sequencing of the spike was done at 25 days after anthesis. The genes for carbohydrate transport and stress response were upregulated in Sunstar as compared to Raj 4079, suggesting that these play a role in intra-spike assimilate distribution. The phenomics method developed may be useful for grain phenotyping and identifying germplasm with low intra-spike variation in grain size for their further validation as parental material in breeding.