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1.
World J Microbiol Biotechnol ; 29(4): 635-43, 2013 Apr.
Artigo em Inglês | MEDLINE | ID: mdl-23184579

RESUMO

We analyzed methylotrophs in Bina natural vegetation (BNV), and revegetated overburden dump of four (ROBD4) and 12 years (ROBD12), at Bina coal mine in Sonbhadra district. The cultured strains identified as Pseudomonas, Acinetobacter, Stenotrophomonas and Cellvibrio (γ-Proteobacteria), Methylophilus, Ralstonia, Burkholderia (α-Proteobacteria) Methylobacterium and Inquilinus (ß-Proteobacteria), Bacillus (Firmicutes) and Flexibacter (Sphingobacteria) in their 16s rRNA gene sequence similarity. The strains differed in citrate, lactose, formate, urea and xylose utilization. Methanol utilization by Stenotrophomonas, Inquilinus, Cellvibrio and Flexibacter is for first time. The preferred N- sources were proline, glutamate and nitrate for most of the strains. All strains tolerated (2.5 % NaCl) and SDS (0.2 %); 16 strains survived in crystal violet (0.01 %) and nine strains in sodium azide (0.02 %. Methylotrophic population trend was BNV > ROBD12 > ROBD4. The presence of majority of strain of BNV at ROBD12 and ROBD4 indicated restoration of soil methylotrophic functional diversity in revegetated dumps.


Assuntos
Bactérias/classificação , Bactérias/isolamento & purificação , Biodiversidade , Compostos Orgânicos/metabolismo , Microbiologia do Solo , Poluentes do Solo/metabolismo , Bactérias/metabolismo , Técnicas de Tipagem Bacteriana , Análise por Conglomerados , DNA Bacteriano/química , DNA Bacteriano/genética , DNA Ribossômico/química , DNA Ribossômico/genética , Dados de Sequência Molecular , Filogenia , RNA Ribossômico 16S/genética , Análise de Sequência de DNA
2.
Anim Biotechnol ; 22(2): 111-7, 2011 Apr.
Artigo em Inglês | MEDLINE | ID: mdl-21500112

RESUMO

The complete genome of a lapinized classical swine fever virus (CSFV) vaccine strain was amplified into nine overlapping fragments by RT-PCR, and nucleotide sequences were determined. Complete genome sequence alignment and phylogenetic analysis indicated 92.6-98.6% identities at the nucleotide level with other reported CSFV strains and could be grouped into subgroup 1.1 along with other attenuated strains of CSFV. The 5'-UTR demonstrated >97.0% nucleotide similarity with most of vaccine CSFV strains from China. Further, its 3'-UTR sequence indicated a length similar to all the CSFV strains from China with >98.0% nucleotide similarity, although high length heterogeneity of 3'-UTR was reported among different CSFV strains. There was 12 nt (TTTTCTTTTTTT) insertion in 3'-UTR similar to other reported attenuated vaccine strains. However, secondary structure of 3'-UTR indicated that Indian CSFV strain requires further passage to obtain a 3'-UTR structure similar to most of the attenuated strains.


Assuntos
Vírus da Febre Suína Clássica , Peste Suína Clássica/virologia , Vacinas Atenuadas/genética , Vacinas Virais/genética , Regiões 3' não Traduzidas/genética , Regiões 5' não Traduzidas/genética , Animais , Sequência de Bases , China , Peste Suína Clássica/genética , Vírus da Febre Suína Clássica/classificação , Vírus da Febre Suína Clássica/genética , Genoma Viral , Índia , Conformação de Ácido Nucleico , Nucleotídeos/genética , Filogenia , RNA Viral/genética , Alinhamento de Sequência , Análise de Sequência de DNA , Homologia de Sequência do Ácido Nucleico , Suínos , Vacinas Virais/classificação
3.
3 Biotech ; 7(6): 357, 2017 Dec.
Artigo em Inglês | MEDLINE | ID: mdl-29038774

RESUMO

Curcuma longa L., commonly known as turmeric, is a rhizomatous herb of the family Zingiberaceae. It is mostly used as a spice, a coloring agent and broadly used in traditional medicine such as Ayurveda, Unani, etc., Turmeric rhizomes interact with a large numbers of rhizosphere-associated microbial species, and some enter the plant tissue and act as endophytes. Both rhizospheric and endophytic species are directly or indirectly involved in growth promotion and disease management in plants and also play an important role in the modulation of morphological growth, secondary metabolite production, curcumin content, antioxidant properties, etc. The present review focuses on the rhizobacterial and endophytic bacterial and fungal populations associated with the turmeric.

4.
Acta Vet Hung ; 54(4): 449-53, 2006 Dec.
Artigo em Inglês | MEDLINE | ID: mdl-17278717

RESUMO

Pasteurella multocida B:2 is responsible for haemorrhagic septicaemia in cattle and buffaloes, causing severe economic losses in the developing countries. In the present study, the ahpA gene of P. multocida B:2 (P52) was cloned, sequenced and compared with the previously reported ahpA gene sequence in P. multocida A:1, which is responsible for its haemolytic phenotype. E. coli DH5a cells were further transformed with recombinant plasmid carrying the ahpA gene from P. multocida B:2 (P52) but SDS-PAGE analysis failed to show the expression of haemolysin protein. Slight haemolysis was albeit observed in horse blood agar plates streaked with recombinant E. coli carrying the ahpA gene. Our study indicates that there is 99.6% similarity and 0.4% divergence between ahpA gene of P. multocida B:2 (P52) and P. multocida A: 1, while membrane topology analysis has predicted that ahpA is an inner membrane protein with two strong hydrophobic regions at the N and C terminals. The presence of significant homology in ahpA sequence in A: 1 and B:2 perhaps suggests a common mechanism of pathogenesis in different species of animals.


Assuntos
Proteínas de Bactérias/genética , Infecções por Pasteurella/veterinária , Pasteurella multocida/isolamento & purificação , Animais , Búfalos , Bovinos , Clonagem Molecular , Infecções por Pasteurella/microbiologia , Pasteurella multocida/classificação , Pasteurella multocida/genética
5.
Vet Microbiol ; 108(3-4): 207-14, 2005 Jul 01.
Artigo em Inglês | MEDLINE | ID: mdl-15916870

RESUMO

Rabies virus glycoprotein is a type I transmembrane protein exposed on the surface on the mature virus particle that induces virus neutralizing antibodies. In the present study, 60 amino acid C-terminal hydrophobic anchor (transmembrane) and cytoplasmic domains of glycoprotein were deleted from full-length glycoprotein and fused with polyhistidine tag. The N-terminal viral signal peptide was also replaced with CD33 signal peptide for efficient secretion in mammalian cells. Following transfection of Madin Darby bovine kidney (MDBK) cells with plasmid encoding this soluble form of glycoprotein, polyclonal populations of stably transfected resistant cells were obtained after G418 selection. The protein was expressed as a glycosylated protein and secreted outside the cells utilizing N-terminal CD33 signal peptide. The secreted soluble glycoprotein was purified from cell culture supernatant by Ni--agarose affinity chromatography utilizing C-terminal polyhistidine tag. Like full-length glycoprotein, the expressed recombinant soluble glycoprotein was found to be immunogenic when injected in rabbits. In this study, we have assessed the potential of recombinant soluble glycoprotein as diagnostic antigen in ELISA and found that this recombinant protein can be used as diagnostic antigen in ELISA for detecting anti-glycoprotein antibodies in immunized host.


Assuntos
Antígenos Virais/imunologia , Glicoproteínas/imunologia , Vírus da Raiva/imunologia , Proteínas do Envelope Viral/imunologia , Animais , Anticorpos Antivirais/sangue , Antígenos Virais/genética , Western Blotting , Cromatografia de Afinidade , DNA Viral/química , DNA Viral/genética , Doenças do Cão/virologia , Cães , Eletroforese em Gel de Poliacrilamida , Ensaio de Imunoadsorção Enzimática , Escherichia coli/genética , Glicoproteínas/genética , Imunização , Reação em Cadeia da Polimerase , Coelhos , Raiva/virologia , Vírus da Raiva/genética , Proteínas Recombinantes/genética , Proteínas Recombinantes/imunologia , Sensibilidade e Especificidade , Transfecção , Proteínas do Envelope Viral/genética
6.
Folia Microbiol (Praha) ; 32(2): 130-2, 1987.
Artigo em Inglês | MEDLINE | ID: mdl-18425684

RESUMO

Heterocyst size variation in Nostoc muscorum has been surveyed in the presence and absence of tyrosine. The heterocyst size exhibited two major peaks under both conditions but one of the peaks shifted towards larger size in tyrosine-containing medium. Heterocysts of larger volume exhibited division in the latter medium which was not observed in medium lacking tyrosine. It is suggested that signals for cell division did not decay following differentiation of heterocyst in the presence of tyrosine.

7.
Comp Immunol Microbiol Infect Dis ; 33(4): 343-53, 2010 Jul.
Artigo em Inglês | MEDLINE | ID: mdl-19200598

RESUMO

Virological and antigenic characteristics of two Indian Peste des Petits Ruminants (PPR) vaccine viruses namely "PPRV-Sungri/96" and "PPRV-AR/87" were investigated. This investigation included, type of cytopathic effect (CPE) produced, one-step growth curve, antigenic reactivity of viral antigens using a panel of 18 monoclonal antibodies (MAbs) and ability of viruses for neutralization using a monoclonal antibody directed to haemagglutinin (H) protein. Findings surprisingly indicated that the PPRV-AR/87 is a fast growing virus with an entirely different pattern of cytopathic effect in Vero cell system. This virus has a relatively short replication cycle of 21h with no clearly defined eclipse phase. Whereas, PPRV-Sungri/96 has a replication cycle of 72h with distinct eclipse phase. Both viruses showed very good antigenic correlation (r=0.823) based on reactivity with 18 MAbs in an indirect ELISA, indicating that they are closely antigenically related. PPRV-AR/87 showed a poor neutralization index with antihaemaglutinin MAb 4B11 and poor reactivity in sandwich ELISA using an anti-nucleocapsid (N) MAb as compared to PPRV-Sungri/96. The findings suggest that both these vaccine viruses can easily be differentiated based on the pattern of cytopathic effect and degree of neutralization using MAb 4B11.


Assuntos
Antígenos Virais/metabolismo , Vírus da Peste dos Pequenos Ruminantes/classificação , Vírus da Peste dos Pequenos Ruminantes/fisiologia , Vacinas Virais , Animais , Antígenos Virais/genética , Chlorocebus aethiops , Efeito Citopatogênico Viral , Índia/epidemiologia , Peste dos Pequenos Ruminantes/epidemiologia , Peste dos Pequenos Ruminantes/virologia , Fatores de Tempo , Células Vero
9.
Vaccine ; 27(19): 2548-53, 2009 Apr 28.
Artigo em Inglês | MEDLINE | ID: mdl-19428860

RESUMO

The combined sheep pox and Peste des Petits ruminants (PPR) vaccine was prepared in lyophilized form containing recommended doses of both vaccine viruses. Safety and immunogenicity of this combined vaccine was evaluated in sheep. Sheep immunized subcutaneously with 1ml of live attenuated vaccine consisting of 10(3)TCID(50) each of sheep pox virus (SPV) Romanian Fanar (RF) strain and Peste des Petits ruminants virus (PPRV-Sungri/96 strain) were monitored for clinical and serological responses for a period of four weeks post immunization (pi) and two week post challenge (pc). Specific antibodies directed to sheep pox virus could be demonstrated by indirect ELISA and serum neutralization test (SNT). Competitive ELISA and SNT were used for demonstration of antibodies to PPR virus. All the immunized animals resisted challenge with virulent SPV or PPRV on day 30pi, while control animals developed characteristic signs of disease. Specific virus could be detected in the unvaccinated control animals after challenge but not from any of the immunized sheep. Combined vaccine was found to be safe and potent as evident from sero conversion as well as challenge studies in sheep. This indicates that component vaccines did not interfere each other and can be used in target population for economic vaccination strategies.


Assuntos
Capripoxvirus/imunologia , Peste dos Pequenos Ruminantes/prevenção & controle , Vírus da Peste dos Pequenos Ruminantes/imunologia , Infecções por Poxviridae/prevenção & controle , Doenças dos Ovinos/prevenção & controle , Vacinas Virais/imunologia , Animais , Anticorpos Antivirais/sangue , Temperatura Corporal , Capripoxvirus/crescimento & desenvolvimento , Chlorocebus aethiops , Túnica Conjuntiva/virologia , Ensaio de Imunoadsorção Enzimática , Injeções Subcutâneas , Cavidade Nasal/virologia , Testes de Neutralização , Vírus da Peste dos Pequenos Ruminantes/crescimento & desenvolvimento , Ovinos , Vacinas Atenuadas/administração & dosagem , Vacinas Atenuadas/efeitos adversos , Vacinas Atenuadas/imunologia , Vacinas Combinadas/administração & dosagem , Vacinas Combinadas/efeitos adversos , Vacinas Combinadas/imunologia , Células Vero , Vacinas Virais/administração & dosagem , Vacinas Virais/efeitos adversos
10.
Vaccine ; 27(15): 2138-43, 2009 Mar 26.
Artigo em Inglês | MEDLINE | ID: mdl-19356617

RESUMO

Rabies is an endemic, fatal zoonotic disease in the developing countries. Oral vaccination strategies are suitable for rabies control in developing countries. Studies were performed to investigate the suitability of poly(lactide-co-glycolide) (PLG) microspheres as an oral delivery system for beta-propiolactone inactivated concentrated rabies virus (CRV). Immune responses induced by encapsulated (PLG+CRV) and un-encapsulated inactivated rabies virus after oral and intraperitoneal route administrations were compared. The anti-rabies virus IgG antibody titer, virus neutralizing antibody (VNA) titers obtained by mouse neutralization test (MNT) and IgG2a and IgG1 titers of mice group immunized orally with PLG+CRV showed significantly (p<0.001) higher response than the group immunized orally with un-encapsulated CRV. There was no significant difference (p>0.05) between groups inoculated by intraperitoneal route. The stimulation index (SI) obtained by lymphoproliferation assay of PLG+CRV oral group also showed significantly (p<0.001) higher response than the group immunized orally with un-encapsulated CRV, suggesting that oral immunization activates Th1-mediated cellular immunity. Immunized mice of all experimental groups were challenged intracerebrally with a lethal dose of virulent rabies virus Challenge Virus Standard (CVS). The survival rates of mice immunized orally with PLG+CRV and CRV alone were 75% and 50%, respectively, whereas intraperitoneally immunized groups showed 100% protection. The overall results of humoral, cellular immune response and survival rates of mice immunized orally with PLG+CRV were significantly (p<0.001) higher than those of mice immunized orally with CRV alone. These data suggest that the PLG encapsulated inactivated rabies virus can be used for oral immunization against rabies.


Assuntos
Microesferas , Poliglactina 910/farmacologia , Vacina Antirrábica/imunologia , Raiva/prevenção & controle , Administração Oral , Animais , Anticorpos Antivirais/sangue , Anticorpos Antivirais/imunologia , Cricetinae , Imunoglobulina G/sangue , Imunoglobulina G/imunologia , Injeções Intraperitoneais , Camundongos , Testes de Neutralização , Poliglactina 910/administração & dosagem , Propiolactona/farmacologia , Vacina Antirrábica/administração & dosagem , Distribuição Aleatória , Vacinas de Produtos Inativados/administração & dosagem , Vacinas de Produtos Inativados/imunologia
11.
Cell Mol Biol (Noisy-le-grand) ; 50(5): 575-84, 2004 Jul.
Artigo em Inglês | MEDLINE | ID: mdl-15559974

RESUMO

Cyanobacterial species composition of fresh water and terrestrial ecosystems and chemical environment of water in Schirmacher Oasis in Continental Antarctica was investigated. Over 35 species of cyanobacteria were recorded. Diazotrophic species both heterocystous and unicellular contributed more than half to the count except in lake ecosystem. The species composition varied among the fresh water as well as terrestrial ecosystems. The physico-chemical analyses of water revealed its poor nurient content which might have supported the growth of diazotrophic cyanobacteria in an Antarctic environment. Among the cyanobacteria Oscillatoria, Phormidium and Nostoc commune were the dominant flora in most of the habitats. The physiological characteristics of isolated cyanobacteria strains indicated that N2-fixation, nitrate uptake, nitrate-reduction, ammonium-uptake, GS-transferase activity and photosynthesis was unaffected at low temperature (5 degrees C) which indicated low temperature adaptation for Antarctic cyanobacteria. This phenomenon was not evident in different strains of tropical origin. The temperature optima for N2-fixation for the different Antarctic cyanobacterial strains was in the range of 15-25 degrees C, nearly 10 degrees C lower than their respective reference strains of tropical origin. Similar results were obtained for cyanobacteria-moss association. The low endergonic activation energy exhibited by the above metabolic activities supported the view that cyanobacteria were adapted to Antarctic ecosystem.


Assuntos
Cianobactérias/fisiologia , Ecologia , Regiões Antárticas , Clima Frio , Nitrato Redutase , Nitrato Redutases/metabolismo , Nitratos , Fixação de Nitrogênio/fisiologia , Fotossíntese/fisiologia , Compostos de Amônio Quaternário/metabolismo , Cloreto de Sódio/metabolismo
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