Infections with multiple high-risk HPV types are associated with high-grade and persistent low-grade intraepithelial lesions of the cervix.

BACKGROUND: Infections with multiple human papillomavirus (HPV) types (mHPV) in Papanicolaou tests have been reported but the histologic correlation and clinical meaning remains debatable. METHODS: The authors prospectively tested 37 HPV types using the Linear Array HPV Genotyping Test and correlated the results to cytology and histology findings in 260 women evaluated from June 2009 to October 2011 and followed for up to 60 months. RESULTS: HPV was detected in 148 of 235 samples (63%) and high-risk HPV was detected in 132 samples (56%). mHPV infection was found to be twice as common as single HPV (sHPV) infection and was detected more frequently in low-grade squamous intraepithelial lesion (LSIL) (48 of 83 samples [58%]) and high-grade squamous intraepithelial lesion or invasive carcinoma (HSIL + (26 of 47 samples [55%]) compared with other categories (P<.001). Of 34 LSIL/cervical intraepithelial neoplasia 1 (CIN1) index cases, 13 of 21 patients with mHPV (61.9%) persisted on CIN1, whereas no histologic abnormality was detected during follow-up in all 12 patients with sHPV infection (high risk or low risk) (P<.001). Eighteen of 20 patients with HSIL/cervical intraepithelial neoplasia 2 (CIN2) (90%) and high-risk mHPV persisted on HSIL+/CIN2 + whereas 6 of 11 patients with sHPV infection did not demonstrate HSIL+/CIN2 + on follow-up (54.5%) (P = .066). Approximately 40% of women with HSIL were infected by high-risk HPV types other than types 16 or 18. CONCLUSIONS: High-risk mHPV infection identified patients with persistent LSIL/CIN1 and may to help identify patients at higher risk of disease progression to HSIL+/CIN2+. Longer follow-up will clarify the role of mHPV testing in patient care. Cancer Cytopathol 2017;125:138-143. © 2016 American Cancer Society.

Determinação do perfil de expressão de miRNAS em carcinomas endometrióides do endométrio / Determination of mirna expression profile in endometrial endometrioid adenocarcinomas

O câncer de corpo uterino é uma das principais doenças ginecológicas, sendo o sexto tumor mais frequente nas mulheres nos países desenvolvidos; no Brasil é o oitavo tumor mais incidente com 4414 casos em 2008 com 2026 mortes. A principal patologia do corpo uterino é o carcinoma do endométrio que corresponde a 90% do total dos tumores que acometem o corpo do útero. Estes se apresentam em dois subtipos histológicos principais (o endometrioide e o seroso) com características histopatológicas e clínicas distintas. As principais alterações moleculares associadas aoscarcinomas de endométrio estão relacionadas a alterações da via do gene PTEN e do gene p53. Recentemente, alterações nas expressões de micro RNAs (miRNA) tem sido demonstradas em diversas neoplasias, inclusive nos carcinomas de endométrio. Porém, ainda não foram estabelecidos perfis de expressão de miRNAs associados aos carcinomas de endométrio em amostras da população brasileira. Diante disso, o este trabalho teve como objetivo determinar o perfil de expressão de miRNAs em 33 amostras de carcinomas de endométrio diagnosticados e tratados em nossa Instituição através da comparação com as expressões de miRNAs em 14 amostras de endométrio não neoplásico (8 amostras de endométrio normal e 6 amostras de pólipos endometriais). Após as análises, observamos um aumento da expressão do miR-590-5p nas amostras tumorais quando comparados ao grupo controle, observamos ainda alteração de expressão em 47 miRNAs quando comparados os grupos tumor e normal. Obtivemos, também, uma “assinatura” de 12 miRNAs diferencialmente expressos em pelo menos 60% das amostras normais...

The uterine body cancer is one of the main gynecological diseases, being the sixth most common tumor in women in developed countries; in Brazil is the eighth most frequent tumor with 4414 cases in 2008 with 2026 deaths. The major pathology is the endometrial carcinoma which corresponds to 90% of all tumors that involve the body of the uterus. They are classified in two major histological subtypes (endometrioid and serous) with different histological and clinical characteristics. The principal molecular changes associated with endometrial carcinomas are related to alterations in the PTEN and p53 gene pathways. Recently, changes in the expression of microRNAs (miRNA) have been demonstrated in many neoplasms, including endometrial carcinomas. However, they have not yet been established miRNA expression profiles associated with endometrial carcinomas in the Brazilian population samples. This study aimed to determine the profile of miRNA expression in 33 samples of endometrial carcinomas diagnosed and treated in our institution by comparison with the expression of miRNAs in 14 endometrial samples nonneoplastic (8 normal endometrial samples and 6 samples of endometrial polyps). After analysis, we observed an increased expression of miR-590-5p in tumor samples when compared to the control group, We also observed changes in expression of 47 miRNAs compared...