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1.
Small ; : e2400780, 2024 Mar 30.
Artigo em Inglês | MEDLINE | ID: mdl-38554020

RESUMO

Developing efficient homojunctions on g-C3N4 promises metal-free photocatalysis to realize truly sustainable artificial photosynthesis. However, current designs are limited by hindered charge separation due to inevitable grain boundaries and random formation of ineffective homojunctions embedded within the photocatalyst. Here, efficient photocatalysis is driven by introducing effective surface homojunctions on chemically and structurally identical g-C3N4 through leveraging its size-dependent electronic properties. Using a top-down approach, the surface layer of bulk g-C3N4 is partially exfoliated to create sheet-like g-C3N4 nanostructures on the bulk material. This hierarchical design establishes a subtle band energy offset between the macroscopic and nanoscopic g-C3N4, generating homojunctions while maintaining the chemical and structural integrities of the original g-C3N4. The optimized g-C3N4 homojunction demonstrates superior photocatalytic degradation of antibiotic pollutants at >96% efficiency in 2 h, even in different real water samples. It achieves reaction kinetics (≈0.041 min-1) up to fourfold better than standalone materials and their physical mixture. Mechanistic studies highlight the importance of the unique design in boosting photocatalysis by effectively promoting interfacial photocarrier manipulation and utilization directly at the point-of-catalysis, without needing co-catalysts or sacrificial agents. This work presents enormous opportunities for developing advanced and green photocatalytic platforms for sustainable light-driven environmental, energy, and chemical applications.

2.
Reprod Domest Anim ; 57(4): 393-401, 2022 Apr.
Artigo em Inglês | MEDLINE | ID: mdl-34967972

RESUMO

The present study compared the outcomes of in vivo embryo production in Morada Nova ewes subjected to either 9-day (G-9SOV , n = 21) or 12-day (G-12SOV , n = 21) progesterone (P4 )-based estruses synchronization protocol coupled with superovulatory treatment with decreasing doses of porcine follicle-stimulating hormone (133 mg of pFSH given over 3 days). Non-surgical embryo recovery (NSER) was performed 6-7 days after the onset of oestrus. Total antral follicle count doubled from the first to the sixth pFSH dose in both groups (p < .05). Oestrus responses did not vary between the two groups of animals (95.2%). Corpora lutea (CL) were detected in 85.0% and 60.0% of ewes that previously manifested oestrus behaviour in G-9SOV and G-12SOV respectively. NSER was successfully completed in 86.2% of ewes that had CL (p > .05). The mean number of CL per ewe/successfully flushed donor ewe was greater (p < .05) in G-12SOV (12.3 ± 1.7/12.1 ± 1.9) than in G-9SOV (7.9 ± 1.4/8.2 ± 1.6). Mean numbers of retrieved blastocysts and viable embryos were greater (p > .05) in G-12SOV (5.8 ± 1.9 and 3.7 ± 1.7) than G-9SOV (3.5 ± 1.1 and 0.8 ± 0.3 respectively). The total follicle count (all follicles ≥2 mm in diameter) at the sixth pFSH dose (at P4 -device removal) was positively correlated (p < .05) with the number of CL (r = .95) and viable embryos (r = .91) in G-12SOV . The ewes with ≥10 Cl (48% of all flushed donors) yielded 80.5% of viable embryos. In summary: (a) Morada Nova ewes from G-12SOV group had better superovulatory responses compared with G-9SOV group; (b) total follicle count at the last pFSH dose was a good predictor of superovulatory responses only in the ewes primed with P4 for 12 days; and (c) animals with ≥10 ovulations are main contributors to viable embryo production in Morada Nova ewes.


Assuntos
Sincronização do Estro , Superovulação , Animais , Corpo Lúteo , Feminino , Hormônio Foliculoestimulante , Folículo Ovariano , Ovinos , Superovulação/fisiologia , Suínos
3.
PLoS Genet ; 14(11): e1007731, 2018 11.
Artigo em Inglês | MEDLINE | ID: mdl-30383789

RESUMO

Evolutionary responses to environmental change depend on the time available for adaptation before environmental degradation leads to extinction. Explicit tests of this relationship are limited to microbes where adaptation usually depends on the sequential fixation of de novo mutations, excluding standing variation for genotype-by-environment fitness interactions that should be key for most natural species. For natural species evolving from standing genetic variation, adaptation at slower rates of environmental change may be impeded since the best genotypes at the most extreme environments can be lost during evolution due to genetic drift or founder effects. To address this hypothesis, we perform experimental evolution with self-fertilizing populations of the nematode Caenorhabditis elegans and develop an inference model to describe natural selection on extant genotypes under environmental change. Under a sudden environmental change, we find that selection rapidly increases the frequency of genotypes with high fitness in the most extreme environment. In contrast, under a gradual environmental change selection first favors genotypes that are worse at the most extreme environment. We demonstrate with a second set of evolution experiments that, as a consequence of slower environmental change and thus longer periods to reach the most extreme environments, genetic drift and founder effects can lead to the loss of the most beneficial genotypes. We further find that maintenance of standing genetic variation can retard the fixation of the best genotypes in the most extreme environment because of interference between them. Taken together, these results show that slower environmental change can hamper adaptation from standing genetic variation and they support theoretical models indicating that standing variation for genotype-by-environment fitness interactions critically alters the pace and outcome of adaptation under environmental change.


Assuntos
Adaptação Biológica/genética , Meio Ambiente , Interação Gene-Ambiente , Variação Genética , Evolução Molecular , Aptidão Genética , Genética Populacional , Mutação , Reprodutibilidade dos Testes , Seleção Genética
4.
Int J Behav Nutr Phys Act ; 16(1): 139, 2019 12 27.
Artigo em Inglês | MEDLINE | ID: mdl-31882021

RESUMO

BACKGROUND: Consumption of certain foods is associated with long-term weight gains and abdominal fat accumulation in healthy, middle-aged and young, non-obese participants. Whether the same foods might be associated with changes in adiposity in elderly population at high cardiovascular risk is less known. OBJECTIVE: Using yearly repeated measurements of both food habits and adiposity parameters, we aimed to investigate how changes in the consumption of specific foods were associated with concurrent changes in weight or waist circumference (WC) in the PREDIMED trial. DESIGN: We followed-up 7009 participants aged 55-70 years at high cardiovascular risk for a median time of 4.8 years. A validated 137-item semi-quantitative Food Frequency Questionnaire was used for dietary assessment with yearly repeated measurements. We longitudinally assessed associations between yearly changes in food consumption (serving/d) and concurrent changes in weight (kg) or WC (cm). RESULTS: Yearly increments in weight were observed with increased consumption (kg per each additional increase in 1 serving/d) for refined grains (0.32 kg/serving/d), red meat (0.24), potatoes (0.23), alcoholic beverages (0.18), processed meat (0.15), white bread (0.07) and sweets (0.04); whereas inverse associations were detected for increased consumption of low-fat yogurt (- 0.18), and low-fat milk (- 0.06). Annual WC gain (cm per each additional increase in 1 serving/d) occurred with increased consumption of snacks, fast-foods and pre-prepared dishes (0.28), processed meat (0.18), alcoholic beverages (0.13), and sweets (0.08); whereas increased consumption of vegetables (- 0.23), and nuts (- 0.17), were associated with reductions in WC. CONCLUSIONS: In this assessment conducted in high-risk subjects using yearly repeated measurements of food habits and adiposity, some ultra-processed foods, refined carbohydrates (including white bread), potatoes, red meats and alcohol were associated with higher weight and WC gain, whereas increases in consumption of low-fat dairy products and plant foods were associated with less gain in weight and WC. TRIAL REGISTRATION: This study was registered at controlled-trials.com with International Standard Randomized Controlled Trial Number (ISRCTN): 35739639. Registration date: 5 October 2005.


Assuntos
Peso Corporal/fisiologia , Doenças Cardiovasculares/epidemiologia , Dieta/métodos , Circunferência da Cintura/fisiologia , Idoso , Animais , Comportamento Alimentar/fisiologia , Feminino , Humanos , Estudos Longitudinais , Masculino , Pessoa de Meia-Idade , Fatores de Risco
5.
Contemp Oncol (Pozn) ; 22(2): 118-123, 2018.
Artigo em Inglês | MEDLINE | ID: mdl-30150890

RESUMO

Epithelioid haemangioendothelioma (EHE) is a rare low-grade vascular neoplasm that is composed of mostly epithelioid cells. EHE may arise as a solitary tumour or in the form of multiple body lesions, and commonly occurs in soft tissues, liver, pleura, lung, peritoneum, lymph nodes, breast, and many other sites. EHE in the cranionasal region is extremely rare. There are very few reports of cases of skull-base EHE. We discuss an extremely rare presentation of an aggressive EHE that originated from the sellar region. Based on literature review, our patient is the first reported case of a giant solitary EHE with prepontine cistern invasion and abducens nerve encroachment mimicking a chondrosarcoma. We treated this rare tumour by near subtotal surgical excision with subsequent radiotherapy, considering that complete tumour resection with free margins in both cavernous sinus and clival region avoiding neural and vascular structure encroachment becomes technically difficult.

6.
Rev Esp Enferm Dig ; 107(7): 449-53, 2015 Jul.
Artigo em Inglês | MEDLINE | ID: mdl-26140642

RESUMO

Endoesophageal brachytherapy is a useful technique for the palliative treatment of dysphagia in advanced oesophageal cancer. This technique offers good results on dysphagia control and quality of life.We report the case of a patient treated with this technique presenting complete response to the dysphagia. We describe endoesophageal brachyterapy technique and we comment on the literature.


Assuntos
Braquiterapia/métodos , Carcinoma de Células Escamosas/radioterapia , Transtornos de Deglutição/etiologia , Neoplasias Esofágicas/radioterapia , Esofagoscopia , Cuidados Paliativos/métodos , Carcinoma de Células Escamosas/complicações , Neoplasias Esofágicas/complicações , Evolução Fatal , Feminino , Humanos , Pessoa de Meia-Idade
7.
Eur Arch Otorhinolaryngol ; 271(2): 373-8, 2014 Feb.
Artigo em Inglês | MEDLINE | ID: mdl-23644939

RESUMO

Few therapeutic options are available for recurrent/metastatic head and neck cancer when progression occurs after initial chemotherapy. We analyzed retrospectively the efficacy of weekly Paclitaxel plus Cetuximab as second line of palliative chemotherapy. Patients with squamous carcinoma of head and neck with documented progression after initial treatment were enrolled. Tumor response was evaluated through the response evaluation criteria in solid tumor criteria. The retrospective analysis focused on overall survival (OS) and progression-free survival (PFS). Between 2008 and 2011, 33 consecutive patients were treated. A response rate of 55% was observed, with median response duration of 5.0 months (95% CI 3.3-11.1). The median PFS was 4.0 months (95% CI 2.9-5.0) and the median OS time was 10.0 months (95% CI 7.9-12.0). Acne-like rash/Folliculitis and chronic anemia were the most common adverse events. A weekly schedule of Paclitaxel plus Cetuximab is a promising regimen for patients with advanced head and neck cancer after failure of platinum-based therapy. Good tolerance of this treatment suggests that would be used in fragile patients.


Assuntos
Protocolos de Quimioterapia Combinada Antineoplásica/uso terapêutico , Carcinoma de Células Escamosas/tratamento farmacológico , Neoplasias de Cabeça e Pescoço/tratamento farmacológico , Cuidados Paliativos , Idoso , Idoso de 80 Anos ou mais , Anticorpos Monoclonais Humanizados/administração & dosagem , Carboplatina , Cetuximab , Cisplatino , Intervalo Livre de Doença , Resistencia a Medicamentos Antineoplásicos , Feminino , Humanos , Masculino , Pessoa de Meia-Idade , Paclitaxel/administração & dosagem , Estudos Retrospectivos , Carcinoma de Células Escamosas de Cabeça e Pescoço , Falha de Tratamento , Resultado do Tratamento
8.
Plants (Basel) ; 13(6)2024 Mar 08.
Artigo em Inglês | MEDLINE | ID: mdl-38592833

RESUMO

Pesticide overuse in agricultural systems has resulted in the development of pest resistance, the impoverishment of soil microbiota, water pollution, and several human health issues. Nonetheless, farmers still depend heavily on these agrochemicals for economically viable production, given the high frequency at which crops are affected by pests. Phytopathogenic insects are considered the most destructive pests on crops. Botanical pesticides have gained attention as potential biopesticides and complements to traditional pesticides, owing to their biodegradability and low toxicity. Plant-based extracts are abundant in a wide variety of bioactive compounds, such as flavonoids, a class of polyphenols that have been extensively studied for this purpose because of their involvement in plant defense responses. The present review offers a comprehensive review of current research on the potential of flavonoids as insecticides for crop protection, addressing the modes and possible mechanisms of action underlying their bioactivity. The structure-activity relationship is also discussed. It also addresses challenges associated with their application in pest and disease management and suggests alternatives to overcome these issues.

9.
PLoS One ; 19(5): e0300599, 2024.
Artigo em Inglês | MEDLINE | ID: mdl-38728243

RESUMO

The aim of this research is to investigate tourist marketing within the embryonic context of the metaverse in order to comprehend the building blocks and the primary technologies employed in the sector. A systematic literature review (SLR) was conducted on 386 articles, with an overall qualitative approach that included 86 references, all of which dealt with the topic of the metaverse and had direct or potential implications for the tourism sector (hotels, restaurants, means of transport, leisure activities and destination itself). The articles are taken from: Science Direct, Taylor & Francis, Emerald, Springer and Google Scholar. The SLR was carried out according to the PRISMA search protocol. The results indicate the technologies that have been most thoroughly studied at the confluence of marketing, tourism, and the metaverse (AI, virtual reality, augmented reality, mixed reality, blockchain, tokens (NFTs) and digital twins). Moreover, they establish the foundational components of tourism marketing in the metaverse for the first time (tourism products, the metaverse as a distribution and branding channel for tourism and, tourist customer as protagonist). Finally, the study exposes research gaps and recommends future directions for exploration (monetization of products in the metaverse, promotion and marketing strategies in the metaverse, new profiles for marketing professionals, policy development that regulates commercial activity in the metaverse).

10.
Anim Reprod Sci ; 261: 107396, 2024 Feb.
Artigo em Inglês | MEDLINE | ID: mdl-38141546

RESUMO

Results with the use of hCG after synchronization protocol are still inconsistent, which may vary according to breed, season, day of application and dose of the drug used. The aim of the present study was to evaluate the functionality of luteal tissue and ovarian perfusion after hCG treatment during early luteal phase. Estrus-synchronized ewes were randomly assigned to receive i.m. injection of 300 IU of hCG (G-hCG; n = 40) or 1 mL of saline (G-Control; n = 32) on Day 7.5 after progesterone withdrawal. Ultrasonographic evaluations of the ovaries and ovarian and iliac arteries were performed on Days 7.5, 10.5, 13.5, and 21.5. The accessory corpus luteum (aCL) formation rate was 52.5% for G-hCG. There was interaction (p > 0.05) for treatment (G-hCG and G-Control), days (7.5, 10.5, 13.5 and 21.5) and PD (Pregnant and Non-pregnant) for the variables of biometric characteristics of the corpus luteum B-Mode and Color Doppler on days 7.5, 10.5, 13.5 and 21.5. There was no difference (p > 0.05) for pregnancy rates and mean fetuses per ewe between the treatment groups. It is concluded that the application of hCG 7.5 days after the hormonal protocol in Morada Nova ewes in a breeding season is efficient in inducing aCL formation and increasing luteal tissue biometry. However, there was no effect on pregnancy rate.


Assuntos
Sincronização do Estro , Luteína , Gravidez , Feminino , Ovinos , Animais , Sincronização do Estro/métodos , Estações do Ano , Luteína/farmacologia , Corpo Lúteo/diagnóstico por imagem , Progesterona/farmacologia , Gonadotropina Coriônica/farmacologia , Ensaios Clínicos Veterinários como Assunto
11.
Foods ; 12(20)2023 Oct 19.
Artigo em Inglês | MEDLINE | ID: mdl-37893727

RESUMO

Citrus consumption translates into large amounts of residue, the disposal of which is associated with environmental issues and high costs. Current trends in citrus waste focus on the extraction of highly valued bioactive compounds via single-compound extraction. There is a lack of knowledge on how these methodologies can be introduced into extraction schemes of bioactive compounds, maximizing the residue potential and reducing its amount. The present work aimed to address this issue by designing a consecutive extraction of pectin and hesperidin from orange peel waste. A novel method for extraction and precipitation of hesperidin with an eco-friendly approach is also presented. After neutral pretreatment, pectin extraction was conducted under acidic conditions, followed by hesperidin extraction with a drastic pH change. Pectin had a high AUA content (66.20 ± 1.25%), meeting the criteria for use in the food industry. The best-tested conditions for hesperidin extraction (30 min, 70 °C, 1:10 (w/v)) provided a yield of 1% and a purity of 84%. The designed extraction scheme shows the potential of citrus waste as a source of bioactive compounds of good quality and high interest in the food industry while following the principles of green chemistry and circular economy.

12.
Ecol Lett ; 15(12): 1423-9, 2012 Dec.
Artigo em Inglês | MEDLINE | ID: mdl-22958079

RESUMO

Scatter-hoarding animals spread out cached seeds to reduce density-dependent theft of their food reserves. This behaviour could lead to directed dispersal into areas with lower densities of conspecific trees, where seed and seedling survival are higher, and could profoundly affect the spatial structure of plant communities. We tested this hypothesis with Central American agoutis and Astrocaryum standleyanum palm seeds on Barro Colorado Island, Panama. We radio-tracked seeds as they were cached and re-cached by agoutis, calculated the density of adult Astrocaryum trees surrounding each cache, and tested whether the observed number of trees around seed caches declined more than expected under random dispersal. Seedling establishment success was negatively dependent on seed density, and agoutis carried seeds towards locations with lower conspecific tree densities, thus facilitating the escape of seeds from natural enemies. This behaviour may be a widespread mechanism leading to highly effective seed dispersal by scatter-hoarding animals.


Assuntos
Comportamento Alimentar/fisiologia , Roedores/fisiologia , Dispersão de Sementes/fisiologia , Árvores/fisiologia , Animais , Ecossistema , Densidade Demográfica
13.
J Pediatr (Rio J) ; 98(6): 641-647, 2022.
Artigo em Inglês | MEDLINE | ID: mdl-35598677

RESUMO

OBJECTIVE: The present study aims to analyze the validity of evidence and internal consistency of an inventory for assessing parenting practices during early childhood. METHOD: Participants were 857 mothers of one-to-42-months children recruited in three cities in the Southeast region and one city in the Midwest region of Brazil. The participants answered a sociodemographic questionnaire with maternal and child data, and the Parenting Styles Inventory for Mothers of Babies (IEPMB). The IEPMB includes 25 questions about positive and negative parenting practices that mothers use to raise their children. An exploratory and confirmatory factor analysis was conducted using the following criteria to indicate adequate model fit: root-mean-square error of approximation (RMSEA) < .08; standardized root mean square residual (SRMR) < .09; comparative fit index (CFI) > 0.90. For the hypothesis-testing method, a comparison between groups using a student's t-test based on the child's age (infants vs. toddlers) and mother's age (adolescents vs. adults) was carried out. RESULTS: The final 11 items model of the measure revealed an adequate overall model fit (RMSEA = 0.04; SRMR = 0.04; CFI = 0.94). The items were grouped into three constructs: Aggressiveness and Emotional Dysregulation, Relaxed Discipline, and Positive Monitoring. Adolescent mothers reported less positive monitoring than adult mothers (p < 0.001). Mothers of toddlers reported more aggressiveness/emotional dysregulation (p < 0.001) and relaxed discipline (p = 0.05) than mothers of infants. CONCLUSIONS: The instrument named from this study as the Parenting Practices Inventory for Mothers of Babies showed evidence for measuring mothers' parenting practices in early childhood and allows the identification of parents who need support.


Assuntos
Educação Infantil , Poder Familiar , Adulto , Adolescente , Feminino , Lactente , Criança , Pré-Escolar , Humanos , Poder Familiar/psicologia , Brasil , Mães/psicologia , Pais , Inquéritos e Questionários
14.
ACS Appl Mater Interfaces ; 14(39): 45005-45012, 2022 Oct 05.
Artigo em Inglês | MEDLINE | ID: mdl-36162132

RESUMO

A liquid-liquid interfacial reaction combines reactants with large polarity disparity to achieve greener and more efficient chemistry that is otherwise challenging in traditional single-phase systems. However, current interfacial approaches suffer from the need for a large amount of solvent/reactant/emulsifier and poor reaction performance arising from intrinsic thermodynamic constraints. Herein, we achieve an efficient interfacial reaction by creating a magnetic-responsive, microscale liquid-liquid interface and exploit its dynamic spinning motion to generate vortex-like hydrodynamic flows that rapidly converge biphasic reactants to the point-of-reaction. Notably, the spinning of this functional interface at 800 rpm boosts the reaction efficiency and its apparent equilibrium constant by > 500-fold and 105-fold, respectively, higher than conventional methods that utilize bulk and/or non-dynamic liquid interfaces, even with external mechanical stirring. By driving reaction equilibrium toward favorable product formation, our unique design offers enormous opportunities to realize efficient multiphasic reactions crucial for diverse applications in chemical synthesis, environmental remediation, and even molecular recycling.

15.
J Appl Anim Welf Sci ; : 1-17, 2022 Nov 07.
Artigo em Inglês | MEDLINE | ID: mdl-36342446

RESUMO

Cervical relaxation (CR) was performed in ewes during diestrus, to prospect a feasible protocol for non-surgical embryo transfer (NSET). In Trial 1, naturally mated ewes (n=13) received CR protocols with estradiol benzoate (EB, 1 mg on D6) and oxytocin (OT, 50 IU on D7) only (G-EB+OT) or associated with human chorionic gonadotropin (hCG, 300 IU on D7, G-EB+OT+hCG) and were compared to non-hormonally treated (G-control) ewes. Estradiol concentration increased (P<0.05) from D6 to D7 in G-EB+OT and G-EB+OT+hCG, then decreased (P<0.05) on D8. The G-EB+OT had reduced (P<0.05) progesterone on D8, and 0% (0/5) pregnancy, while G-control had 100% (3/3) conception rate. The treatment with hCG mitigated the progesterone drop on D8 and resulted in 50% (2/4) conception rate. In Trial 2, cervical transposing success after treatment with EB, OT, and hCG on D6 and D7 was assessed in nulliparous (n=25) and multiparous (n=30) ewes. The EB+OT+hCG protocol allowed efficient cervical transposition (~78% of cervical transposition success into ~5 min) in nulliparous and multiparous females and did not alter total and blood perfusion areas of corpora lutea (CL). The use of EB+OT+hCG was efficient to ensure effective cervical transposing in nulliparous and multiparous ewes, with no apparent deleterious effect on CL function, and may contribute to animal welfare, allowing a less invasive NSET procedure.

16.
Animals (Basel) ; 13(1)2022 Dec 27.
Artigo em Inglês | MEDLINE | ID: mdl-36611712

RESUMO

The objective of the study was to evaluate energy or energy/protein supplementation in the ewe diet, in the last third of gestation, on maternal placental and endocrine characteristics, as well as its effects on the behavior of neonatal lambs and productive performance until weaning. A total of 128 ewes were used, and the experimental diet was fed from 100 days gestation until lambing, with the birth of 172 lambs. The ewes were distributed into three treatments: CTL (control, n = 43) with metabolizable energy (ME) and crude protein (CP) intake according to NRC (1985); ME (energy supplementation, n = 44) plus 21% ME; and MECP (energy/protein supplementation, n = 41) plus 26% ME and CP. Body weight, body condition score, serum hormone concentrations, placental characteristics, lamb performance and behavior, and production efficiency of the ewe from lambing to weaning were measured. ME and MECP ewes were heavier before (p = 0.006) and just after lambing (p = 0.002) and had higher serum triiodothyronine (p = 0.001) and cortisol (p = 0.004) concentrations on the day of lambing. ME ewes had higher placental efficiency (p = 0.036) and lower total cotyledon weight (p = 0.011). ME and MECP diets increased both lamb birth weight (p = 0.015) and weaning weight (p = 0.009). Production efficiency at birth and at weaning was not influenced (p > 0.05) by treatments. Lamb behavior was influenced by the ME and MECP diets, reducing the time to kneel (p ≤ 0.05), to try to stand (p ≤ 0.05), and the latency to stand (p ≤ 0.005). It is concluded that overnutrition in the last third of gestation was positive for the ovine production system, with improved production rates, hormonal profile, placental characteristics, and neonatal behavior.

17.
Biopreserv Biobank ; 19(5): 360-368, 2021 Oct.
Artigo em Inglês | MEDLINE | ID: mdl-33769085

RESUMO

This study assessed the feasibility of in vivo embryo production and nonsurgical embryo recovery (NSER) in Morada Nova ewes (an endangered native Brazilian breed of sheep) subjected to different estrus synchronization and/or superovulation protocols. Ewes received intravaginal sponges soaked with 60 mg medroxyprogesterone acetate (MAP), which were kept in place for six (G6; n = 12), nine (G9; n = 12), or 12 (G12; n = 12) days. Half of the ewes in each group remained estrus synchronized only (SYNCH) and the other half was superovulated (SOV) with 133 mg porcine follicle-stimulating hormone (pFSH). There were no differences (p > 0.05) in antral follicle counts determined with ultrasonography 60 hours before MAP sponge removal (or at the time of the first pFSH dose) among G6 (6.4 ± 0.9), G9 (6.2 ± 0.7), and G12 (5.5 ± 0.6). Estrus responses and NSER success rates did not vary (p > 0.05) among the three progestin-treatment groups of ewes for either estrus-induced or superovulated animals. The onset of estrus occurred 10-12 hours later (p < 0.01) in G9SYNCH ewes compared with G6SYNCH and G12SYNCH, and the duration of estrus was ∼19 hours greater (p < 0.01) in G9SOV than in G6SOV. The average duration of the NSER procedure was 32.6 ± 1.3 minutes. At least one structure was recovered in 85.7% of synchronized and in 87.5% of superovulated ewes. Viable embryo recovery rates were also similar (p > 0.05) for G6 (1.0 ± 0.3 and 2.5 ± 1.5), G9 (1.3 ± 0.5 and 4.8 ± 2.0), and G12 groups (1.0 ± 0.3 and 4.8 ± 2.3; estrus-synchronized and superovulated ewes, respectively). In conclusion, progestogen pretreatment of different durations and NSER can be employed in Morada Nova ewes, resulting in reasonable viable embryo recovery rates in both estrus-synchronized and superovulated animals. Therefore, both techniques are suitable for use in commercial settings as well as small ruminant conservation programs.


Assuntos
Sincronização do Estro , Estro , Animais , Colo do Útero , Feminino , Hormônio Foliculoestimulante , Ovinos , Superovulação
18.
Anim Reprod Sci ; 223: 106644, 2020 Dec.
Artigo em Inglês | MEDLINE | ID: mdl-33197710

RESUMO

This study was conducted in ewes to assess effects of human chorionic gonadotropin (hCG) administration after imposing an estrous induction treatment regimen. Ewes (n = 115) were treated with a 60 mg medroxyprogesterone-intravaginal-sponge for 6 d plus 200 IU of equine chorionic gonadotropin (eCG) im and 37.5 µg d-cloprostenol im 36 h before sponge removal (Day 0). After natural mating, ewes having at least one corpus luteum (CL; n = 108) were administered either 1 mL of saline (G-Control; n = 53) or 300 IU of hCG (G-hCG; n = 55) on Day 7.5 after sponge removal (Day 0). Ovarian ultrasonography and blood collection were performed on Days 7.5, 13.5, 17.5, 21.5, and 30.5. Accessory CL (aCL) were observed in 81.5 % (G-hCG) and 0.0 % (G-Control) of ewes (P = 0.0001). Diameter, area, and volume of luteal tissue were greater (P < 0.05) in G-hCG from Day 13.5 to 30.5. Progesterone (P4) concentrations were greater (P < 0.05) on Days 13.5, 17.5, 21.5 and 30.5 for ewes of the G-hCG group. Pregnancy percentage was similar (P = 0.25) between groups [47.1 % (G-control) compared with 60.0 % (G-hCG)], although total number of lambs produced by estrous synchronized ewes was greater (P = 0.005) in ewes of the G-hCG group (90.9 % compared with 66.0 %). In conclusion, hCG administration 7.5 days after sponge removal from Morada Nova ewes during the non-breeding season is an effective treatment to induce aCL formation, improve luteal tissue biometry and P4 concentrations, and to enhance the total number of lambs born.


Assuntos
Gonadotropina Coriônica/farmacologia , Corpo Lúteo/efeitos dos fármacos , Sincronização do Estro/efeitos dos fármacos , Ovinos , Animais , Gonadotropina Coriônica/administração & dosagem , Cloprostenol/farmacologia , Anticoncepcionais Orais Hormonais/farmacologia , Esquema de Medicação , Feminino , Humanos , Luteolíticos/farmacologia , Medroxiprogesterona/administração & dosagem , Medroxiprogesterona/farmacologia , Gravidez , Progesterona/sangue , Substâncias para o Controle da Reprodução/administração & dosagem , Substâncias para o Controle da Reprodução/farmacologia
19.
Estud. Psicol. (Campinas, Online) ; 40: e210011, 2023. tab
Artigo em Inglês | LILACS, INDEXPSI | ID: biblio-1506234

RESUMO

Objective We compared parental educational practices and emotional health of 62 mothers of students from nuclear (G1) and non-nuclear families (G2). Method We used an inventory of parenting practices and scales for assessment of anxiety, stress and depression. Results G1 showed significantly higher means for Moral Behavior. Positive correlations between negative parenting educational practices and clinical indicators for maternal emotional health were perceived in both groups and more significantly for G2. Younger mothers in G1 indicated more signs of state anxiety and for G2 the lower socioeconomic level indicated more signs of stress and trait anxiety and less depression. Conclusion The data can contribute to the planning of interventions.


Objetivo Comparar e relacionar práticas educativas parentais e saúde emocional de 62 mães de escolares de famílias nucleares (G1) e não nucleares (G2). Método Utilizou-se um inventário de práticas parentais e escalas para avaliação da ansiedade, estresse e depressão. Resultados O G1 apresentou significativamente médias superiores para Comportamento Moral. Correlações positivas entre as práticas educativas parentais negativas e indicadores clínicos para saúde emocional materna foram percebidos em ambos os grupos e de forma mais expressiva para G2. Mães mais jovens em G1 indicaram mais sinais de ansiedade-estado. Já para G2, o nível socioeconômico inferior indicou mais sinais de estresse e ansiedade-traço e menos depressão. Conclusão Os dados podem contribuir para o planejamento de intervenções.


Assuntos
Família , Saúde Mental , Comportamento Materno
20.
Psicol. teor. prát ; 24(2): 12525, 14.06.2022.
Artigo em Inglês, Português | LILACS | ID: biblio-1434961

RESUMO

Apesar do crescente avanço no uso de instrumentos para a avaliação da saúde emocional nos períodos perinatal e puerperal, destaca-se que a maioria das ferramentas utilizadas não possui evidências psicométricas para a população de gestantes. A Escala de Depressão, Ansiedade e Estresse (EDAE-21) possibilita a mensuração simultânea de níveis de ansiedade, estresse e depressão. O estudo objetivou avaliar a consistência interna da EDAE-21, a validade convergente da escala em relação ao IDATE, à EEP e ao BDI-II, e a estabilidade das avaliações em um intervalo de até 12 semanas. Participaram da pesquisa 53 gestantes (primeira avaliação) e 35 gestantes (segunda avaliação). A escala apresentou índices de consistência interna, estabilidade e convergência adequados à população investigada. A EDAE-21 pode auxiliar profissionais e pesquisadores da saúde, proporcionando agilidade no diagnóstico de indicadores emocionais desfavoráveis em diferentes períodos gestacionais.


Despite the increasing progress in the use of instruments to assess emotional health in the perinatal and puerperal periods, it is emphasized that most of the tools used do not have psychometric evidence for the population of pregnant women. The Depression, Anxiety and Stress Scale (DASS-21) enables the measurement of anxiety, stress, and depression levels, simultaneously. The study aimed to assess the internal consistency of the DASS-21, the convergent validity of the scale in relation to the State-Trait Anxiety Inventory (STAI), the Beck Depression Inventory ­ second edition (BDI-II), and the Perceived Stress Scale (PSS) and the stability of the evaluations in a range of up to 12 weeks. Fifty-three (first evaluation) and 35 pregnant women (second evaluation) participated in the research. The scale showed adequate internal consistency, stability, and convergence rates for the investigated population. The DASS-21 can help practitioners and researchers of health, providing agility in the diagnosis of unfavorable emotional indicators in different gestational periods


A pesar del creciente progreso en el uso de instrumentos para evaluar la salud emocional en el período perinatal y puerperal, se destaca que la mayoría de las herramientas utilizadas no tienen evidencia psicométrica para mujeres embarazadas. La Escala de Depressión, Ansiedad y Estrés (EDAE-21) mide simultáneamente los niveles de ansiedad, estrés y depresión. El estudio evaluó la consistencia interna del EDAE-21, la validez convergente de la escala en relación con el STAI, el BDI-II y el EEP y la estabilidad de las evaluaciones en un intervalo de hasta 12 semanas. 53 mujeres embarazadas (primera evaluación) y 35 mujeres embarazadas (segunda evaluación) participaron en la investigación. La escala presentó valores de consistencia interna, estabilidad y convergencia adecuados para la población investigada. El EDAE-21 puede ayudar a los profesionales de la salud y a los investigadores, proporcionando agilidad en el diagnóstico de indicadores emocionales desfavorables en diferentes períodos de gestación.


Assuntos
Humanos , Feminino , Ansiedade , Estresse Fisiológico , Gestantes , Depressão , Psicometria , Brasil , Assistência Perinatal , Período Pós-Parto , Saúde Materna
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