Relationships between diatom metrics based on species nutrient traits and agricultural land use.

We assessed how diatom metrics were related to different ranges of agricultural land use. Diatom assemblage composition, nutrients, and landscape characteristics were determined at 232 sites in eight agriculturally dominated study areas of the continental United States. Two regional groups based on differences in diatom relations to human disturbance were determined. Changes in diatom species composition were related to nutrients, pH, and conductivity in the eastern study areas (due to more wetlands) and more exclusively to nutrients in the west-central study areas. Homogenization of diatom flora among streams was related to high agricultural disturbance at this transcontinental scale. Species traits were developed separately for the east and west-central study groups and calculated two ways: indicator species analysis for taxa in low and high TN or TP conditions and weighted average partial least squares models of TN and TP concentration. These diatom metrics were significantly related to many indicators of agricultural land use in watersheds, especially percent row crops. Further analysis was conducted on only the west-central region due to its larger sample size. Overall, diatom metrics using species responses to N gradients were better related to agricultural land use than were species responses to P gradients. Most nutrient-based diatom metrics changed greatly in response to low ranges of percent row crops, but only a few high N diatom metrics responded to high row crop conditions. The greater response of diatoms to changes in low agriculture conditions may be due to past diatom evolution occurring when most waters had low nutrient conditions.

The green alga, Cladophora, promotes Escherichia coli growth and contamination of recreational waters in Lake Michigan.

A linkage between Cladophora mats and exceedances of recreational water quality criteria has been suggested, but not directly studied. This study investigates the spatial and temporal association between Escherichia coli concentrations within and near Cladophora mats at two northwestern Lake Michigan beaches in Door County, Wisconsin. Escherichia coli concentrations in water underlying mats were significantly greater than surrounding water (p < 0.001). Below mat E. coli increased as the stranded mats persisted at the beach swash zone. Water adjacent to Cladophora mats had lower E. coli concentrations, but surpassed EPA swimming criteria the majority of sampling days. A significant positive association was found between E. coli concentrations attached to Cladophora and in underlying water (p < 0.001). The attached E. coli likely acted as a reservoir for populating water underlying the mat. Fecal bacterial pathogens, however, could not be detected by microbiological culture methods either attached to mat biomass or in underlying water. Removal of Cladophora mats from beach areas may improve aesthetic and microbial water quality at affected beaches. These associations and potential natural growth of E. coli in bathing waters call into question the efficacy of using E. coli as a recreational water quality indicator of fecal contaminations.

Effects of excess copper on growth of wild rice (Zizania palustris) seedlings tested in reconstituted and natural waters.

Dissolved copper was toxic to wild rice (Zizania palustris) seedlings when exposed in water from the seed collection site of Swamp Creek, Crandon, Wisconsin, USA, and in laboratory-prepared artificial or reconstituted water. Seeds for the study were harvested, then held through a portion of dormancy, in Swamp Creek. After 60 days they were shipped to a laboratory, chilled, and tested with copper after germination. The end point of the tests was net gain in wet weight of the seedlings; additionally, a pronounced reduction in root development was observed. Using measured concentrations, the lowest no-observable-effect concentration (NOEC) in our study was 37 microg/liter in Swamp Creek water and the lowest-observable-effect concentration (LOEC) was 59 microg/liter. However, it appeared that there was a point at which concentrations of copper above 400 microg/liter did not result in any measurable effect or exhibit a definitive dose-response. Because the results in Swamp Creek water were more relevant to the possibility of local metals additions and the association of reduced seedling growth by copper was more powerful in this water, we derived an equation to express the relationship between copper concentration and toxicity for Swamp Creek water. As an example, we would expect a 3.0% reduction in seedling growth at 5.0 microg/liter copper. Seedling roots were particularly affected and the resultant plants may be less well anchored and more susceptible to dislodging than plants not exposed to copper. Further refinement of the methodology may be used to address effects of other contaminants impacting rice beds in North America.