RESUMO
Consumer health risk assessment for feed additives is based on the estimated human exposure to the additive that may occur in livestock edible tissues compared to its hazard. We present an approach using alternative methods for consumer health risk assessment. The aim was to use the fewest possible number of animals to estimate its hazard and human exposure without jeopardizing the safety upon use. As an example we selected the feed flavoring substance piperine and applied in silico modeling for residue estimation, results from literature surveys, and Read-Across to assess metabolism in different species. Results were compared to experimental in vitro metabolism data in rat and chicken, and to quantitative analysis of residues' levels from the in vivo situation in livestock. In silico residue modeling showed to be a worst case: the modeled residual levels were considerably higher than the measured residual levels. The in vitro evaluation of livestock versus rodent metabolism revealed no major differences in metabolism between the species. We successfully performed a consumer health risk assessment without performing additional animal experiments. As shown, the use and combination of different alternative methods supports animal welfare consideration and provides future perspective to reducing the number of animals.
Assuntos
Alcaloides/efeitos adversos , Ração Animal/efeitos adversos , Benzodioxóis/efeitos adversos , Aromatizantes/efeitos adversos , Piperidinas/efeitos adversos , Alcamidas Poli-Insaturadas/efeitos adversos , Animais , Galinhas , Simulação por Computador , Qualidade de Produtos para o Consumidor , Estudos de Viabilidade , Feminino , Masculino , Ratos , Ratos Wistar , Medição de Risco/métodos , SegurançaRESUMO
Transgenic Drosophila that expressed either luciferase or green fluorescent protein driven from the promoter of the clock gene period were used to monitor the circadian clock in explanted head, thorax, and abdominal tissues. The tissues (including sensory bristles in the leg and wing) showed rhythmic bioluminescence, and the rhythms could be reset by light. The photoreceptive properties of the explanted tissues indicate that unidentified photoreceptors are likely to contribute to photic signal transduction to the clock. These results show that autonomous circadian oscillators are present throughout the body, and they suggest that individual cells in Drosophila are capable of supporting their own independent clocks.
Assuntos
Relógios Biológicos/fisiologia , Ritmo Circadiano/fisiologia , Drosophila/fisiologia , Proteínas Nucleares/fisiologia , Células Fotorreceptoras de Invertebrados/fisiologia , Animais , Animais Geneticamente Modificados , Encéfalo/fisiologia , Células Quimiorreceptoras/fisiologia , Escuridão , Drosophila/genética , Proteínas de Drosophila , Regulação da Expressão Gênica , Genes de Insetos , Proteínas de Fluorescência Verde , Luz , Transdução de Sinal Luminoso , Luciferases/genética , Luminescência , Proteínas Luminescentes/genética , Proteínas Nucleares/genética , Proteínas Circadianas Period , Regiões Promotoras Genéticas , Receptores de Superfície Celular , Proteínas Recombinantes de FusãoRESUMO
The rapid turnover of luciferase and the sensitive, non-invasive nature of its assay make this reporter gene uniquely situated for temporal gene expression studies. To determine the in vivo regulatory pattern of the Drosophila clock gene period (per), we generated transgenic strains carrying a luciferase cDNA fused to the promoter region of the per gene. This has allowed us to monitor circadian rhythms of bioluminescence from pacemaker cells within the head for several days in individual living adults. These high time-resolution experiments permitted neuronal per transcription and opens the door to vastly simplified experiments in general chronobiology and studies of temporally regulated transcription in a wide range of experimental systems.
Assuntos
Drosophila/genética , Luciferases/genética , Neurônios/metabolismo , Proteínas Nucleares/genética , Transcrição Gênica , Animais , Animais Geneticamente Modificados , Ritmo Circadiano , DNA Complementar , Proteínas de Drosophila , Genes Reporter , Luciferases/metabolismo , Medições Luminescentes , Proteínas Circadianas Period , Regiões Promotoras Genéticas , RNA Mensageiro/metabolismo , Proteínas Recombinantes de FusãoRESUMO
To determine the in vivo regulatory pattern of the clock gene period (per), the authors recently developed transgenic Drosophila carrying a luciferase cDNA fused to the promoter region of per. They have now carried out noninvasive, high time-resolution experiments allowing high-throughput monitoring of circadian bioluminescence rhythms in individual living adults for several days. This immediately solved several problems (resulting directly from individual asynchrony within a population) that have accompanied previous biochemical experiments in which groups of animals were sacrificed at each time point. Furthermore, the authors have developed numerical analysis methods for automatically determining rhythmicity associated with bioluminescence records from single flies. This has revealed some features of per gene transcription that were previously unappreciated and provides a general strategy for the analysis of rhythmic time series in the study of molecular rhythms.
Assuntos
Ritmo Circadiano , Drosophila/fisiologia , Genes de Insetos/fisiologia , Transcrição Gênica/fisiologia , AnimaisRESUMO
We have investigated the utility of the green fluorescent protein (GFP) as a marker for gene expression in living adult Drosophila melanogaster (Dm) and cultured plant and mammalian cells. Using Dm, we generated transgenic flies bearing a glass-responsive gfp fusion gene to test the utility of GFP as a spatial reporter. In the adult living fly, GFP is clearly visible in the ocelli and the eye. We have optimized the use of filters for distinguishing the GFP signal from abundant autofluorescence in living Dm. In addition, we have used GFP to identify photoreceptor cells in pupal eye cultures that have been fixed and stained according to standard histological procedures. GFP was also detected in individual living plant cells following transient transfection of soybean suspension cultures, demonstrating that GFP is an effective transformation marker in plant cells. Similarly, transient transfection of mammalian cells with a modified form of GFP, S65T, allowed detection of single living cells expressing the reporter. This modified form of GFP gave a robust signal that was resistant to photobleaching. We then used a CellScan system exhaustive photon reassignment (EPR) deconvolution algorithm to generate high-resolution three-dimensional images of GFP fluorescence in the living cell.
Assuntos
Proteínas de Drosophila , Drosophila melanogaster/genética , Expressão Gênica , Genes Reporter , Proteínas Luminescentes/genética , Animais , Animais Geneticamente Modificados , Células Cultivadas , Citomegalovirus/genética , Proteínas de Ligação a DNA/genética , Proteínas de Ligação a DNA/metabolismo , Proteínas de Fluorescência Verde , Humanos , Proteínas Luminescentes/metabolismo , Ratos , Cifozoários , Glycine max/citologia , Células Tumorais CultivadasRESUMO
Subcutaneous injections of 40 mumol/kg of CdCl2 given to rats on day 18 of pregnancy produced a high incidence of fetal death and placental necrosis. Fetuses directly injected with CdCl2 in utero were resistant to cadmium levels far in excess of fetal levels associated with fetal death following maternal injection. Thus cadmium-induced fetal death was not the result of a direct effect of cadmium on the fetus. Similarly, exposure of fetuses or dams to Cd-metallothionein did not produce fetal death. Placental histological changes and high placental accumulations of cadmium suggested placental mechanisms for the toxicity. Histological changes were observed as early as 12 hours after injection and were characteristic of local circulatory responses. Blood flow measurements with radiolabelled microspheres indicated that uteroplacental blood flow was decreased 40 per cent and 75 per cent at 12-16 hours and 18-24 hours after injection. Studies on the initial responses of the placenta to cadmium exposure revealed that biochemical and ultrastructural changes could be observed in the placenta prior to alterations in blood flow and fetal death. No ultrastructural changes were observed in the uterine vascular endothelium. Thus cadmium-induced fetal death was not the result of direct effects of cadmium but may be the result of a placental effect of the heavy metal. A proposed mechanism for the induction of fetal death is that high placental accumulations of cadmium result in trophoblastic damage which leads to a local circulatory response to the injured tissues and a decrease in uteroplacental blood flow. It is the decrease in nutrient and oxygen transport to the fetus that results from trophoblastic damage and blood flow alterations that ultimately induce fetal death.
Assuntos
Cádmio/toxicidade , Feto/efeitos dos fármacos , Placenta/efeitos dos fármacos , Animais , Feminino , Metalotioneína/farmacologia , Placenta/irrigação sanguínea , Placenta/patologia , Placenta/ultraestrutura , Gravidez , Ratos , Ratos Endogâmicos , Útero/irrigação sanguíneaRESUMO
The presence of structurally diverse chemicals as contaminants in the environment has led to concerns regarding their possible endocrine disturbing effects. Recently, some ultraviolet absorbing components of sunscreen preparations have given positive responses in assays monitoring estrogen-like activity both in vitro and in vivo. Consequently, two recently developed sunscreen components, Tinosorb M-active and Tinosorb S, were evaluated using the in vitro estrogen and androgen receptor competitive binding assays. Neither compound gave a positive response in either of the assays, consistent with the large molecular dimensions of each chemical disfavoring binding to the hormone receptors. Both of the chemicals were inactive in immature rat uterotrophic assays conducted using the subcutaneous route of administration. It is concluded that neither of these agents possess intrinsic estrogenic/antiestrogenic or androgenic/antiandrogenic activity. The several positive control chemicals evaluated gave the expected positive responses in the assays used.
Assuntos
Fenóis/toxicidade , Receptores Androgênicos/efeitos dos fármacos , Receptores de Estrogênio/efeitos dos fármacos , Protetores Solares/toxicidade , Triazinas/toxicidade , Útero/efeitos dos fármacos , Animais , Ligação Competitiva/efeitos dos fármacos , Glândulas Endócrinas/efeitos dos fármacos , Exposição Ambiental , Estradiol/metabolismo , Feminino , Injeções Subcutâneas , Masculino , Fenóis/química , Ratos , Ratos Endogâmicos , Receptores Androgênicos/metabolismo , Receptores de Estrogênio/metabolismo , Protetores Solares/química , Testosterona/metabolismo , Triazinas/química , Raios Ultravioleta , Útero/metabolismoRESUMO
A new regulatory element necessary for the correct temporal expression of the period (per) gene was identified by monitoring real-time per expression in living individual flies carrying two different period-luciferase transgenes. luciferase RNA driven from only the per promoter was not sufficient to replicate the normal pattern of per RNA cycling; however, a per-luc fusion RNA driven from a transgene containing additional per sequences cycled identically to endogenous per. The results indicate the existence of at least two circadian-regulated elements--one within the promoter and one within the transcribed portion of the per gene. Phase and amplitude analysis of both per-luc transgenes revealed that normal per expression requires the regulation of these elements at distinct phases and suggests a mechanism by which biological clocks sustain high-amplitude feedback oscillations.