An Unusual and Rare Case of Food-dependent Exercise-induced Anaphylaxis Caused by Ingestion of Potatoes.

Food-dependent exercise-induced anaphylaxis (FDEIA) is an unusual and under-recognized form of exercise induced anaphylaxis, which usually occurs if exercise takes place within a few hours after ingestion of sensitizing food, but in some cases may also arise if food follows the exercise. We report a case of a 31-year-old woman who presented to our Department with a history of repeated episodes of acute allergic reactions, triggered by physical activity, after consumption of various vegetables and legumes. This is the first described case of FDEIA in Bulgaria. We believe that there are many other undiagnosed cases, because for the correct recognition of this condition, it is essential to be familiar with the symptoms and combination of factors.

The effectiveness of levocetirizine and desloratadine in up to 4 times conventional doses in difficult-to-treat urticaria.

BACKGROUND: H(1)-antihistamines are first line treatment of chronic urticaria, but many patients do not get satisfactory relief with recommended doses. European guidelines recommend increased antihistamine doses of up to 4-fold. OBJECTIVE: To provide supportive evidence for the European guidelines. METHODS: Eighty tertiary referral patients with chronic urticaria (age range, 19-67 years) were randomized for double-blind treatment with levocetirizine or desloratadine (40/40). Treatment started at the conventional daily dose of 5 mg and then increased weekly to 10 mg, 20 mg, or 20 mg of the opposite drug if relief of symptoms was incomplete. Wheal and pruritus scores, quality of life, patient discomfort, somnolence, and safety were assessed. RESULTS: Thirteen patients became symptom-free at 5 mg (9 levocetirizine vs 4 desloratadine), compared with 28 subjects on the higher doses of 10 mg (8/7) and 20 mg (5/1). Of the 28 patients nonresponsive to 20 mg desloratadine, 7 became symptom-free with 20 mg levocetirizine. None of the 18 levocetirizine nonresponders benefited with 20 mg desloratadine. Increasing antihistamine doses improved quality of life but did not increase somnolence. Analysis of the effect of treatment on discomfort caused by urticaria showed great individual heterogeneity of antihistamine responsiveness: approximately 15% of patients were good responders, approximately 10% were nonresponders, and approximately 75% were responders to higher than conventional antihistamine doses. No serious or severe adverse effects warranting discontinuation of treatment occurred with either drug. CONCLUSION: Increasing the dosage of levocetirizine and desloratadine up to 4-fold improves chronic urticaria symptoms without compromising safety in approximately three quarters of patients with difficult-to-treat chronic urticaria.

Significance of molecular-cytogenetic aberrations for the achievement of first remission in de novo acute myeloid leukemia.

OBJECTIVE: The majority of adults diagnosed with acute myeloid leukemia (AML) display acquired cytogenetic aberrations at presentation. In this article, we present the major cytogenetic findings regarding AML and review their clinical significance for achievement of the first complete remission. METHODS: We studied 71 adult patients with de novo AML, without previous myelodysplasia or alkylating therapy. Conventional cytogenetics and FISH were performed on bone marrow cells. The patients with AML were assigned to 12 subgroups according to established data for cytogenetic, molecular and general laboratory results. The selection of the analyzed parameters is consistent with internationally accepted "prognostic factors" in adult AML. RESULTS: Complete remission upon induction therapy was achieved in 40% of cases (in a mean period of 2.3 months from therapy initiation). The patients with t(15;17) PML-RARA and inv(16)/CBFbeta-MYH11ë demonstrated the highest frequency of complete remission. Patients with hypodiploidy, t(9;22)/bcr-abl and complex karyotypes were therapy-resistant or died within the first three months after AML diagnosis. CONCLUSION: Molecular-cytogenetic findings have an important significance for achievement of first complete remission. However, laboratory and biologic features (age, WBC and LDH) and type of AML have a large influence on the disease outcome.

IFN-γ Attenuates Spontaneous Lymphocyte Proliferation by Fuelling Regulatory T Cells in HIV-1-Infected Patients.

HIV infection is characterized by a high degree of immune activation. It has an impact on CD4 cell count and populations' distribution and function. T regulatory cells (Tregs) were found to play a controversial role in the course of infection because of their beneficial effect on the degree of immune activation and unfavorable influence on the antigen-specific responses. The goal of the present work was to study the relationship among interferon-γ (IFN-γ), spontaneous lymphocyte proliferation, and regulatory T cells in HIV patients receiving therapy. Three lymphocyte populations, isolated after a stepwise magnetic separation from 17 individuals, were investigated-peripheral blood lymphocytes, CD4+CD45RA- (CD4+TM), and CD4+CD45RA-CD25- (TMCD25depl.) cells. The spontaneous, phytohemagglutinin (PHA) and HIV-1p24Ag-stimulated IFN-γ production and the spontaneous lymphocyte proliferation were evaluated. The potential of Tregs to establish a productive infection was determined by measurement of free HIV-1p24 antigen. Two types of constellations among subsets were found. In the first one (in 11 subjects), the spontaneous INF-γ inversely correlated with the spontaneous proliferation in all fractions (r = -0.9, p < 0.001). Conversely, in the second group (six subjects), no associations between the selected parameters were observed. The overall increase in p24-stimulated IFN-γ from TMCD25depl. cells was weak. Four samples: one in Tregs and three in TMCD25depl. cells were positive for the free p24 antigen. No association with the CD4+ T cell count, percentage of Tregs, and stage of infection was determined. In conclusion, our results demonstrate that IFN-γ could impact the proliferative capacity of non-Treg cells by fuelling Tregs. Furthermore, Tregs may control the spontaneous lymphocyte proliferation, but are less powerful in the suppression of Ag-specific IFN-γ production from non-Treg lymphocytes. The direct viral influence on Treg functions should be also considered.

Molecular-cytogenetic aberrations in B-cell adult acute lymphoblastic leukemia (B-ALL) - frequency and correlation with immunophenotype.

B-cell acute lymphoblastic leukemia (B-ALL) accounts for 20-30% of acute leukemias in adults. Combined application of data from immunophenotyping, karyotyping and molecular analyses allows a better understanding of this heterogeneous disease. We studied 30 adult patients with newly diagnosed B-ALL by conventional cytogenetics, fluorescent in situ hybridization (FISH) and immunophenotyping analyses. We report statistically significant prevalence of structural aberrations (43%) over numerical changes (17%) (p=0.02). The most frequent structural changes were t(9;22)(q34;q11)/bcr-abl-17%, t(8q24)/C-MYC-10%, t(11q23)/MLL-6%, del 4p-6%, del12p-3%, and t(1;19)-3%. Complex karyotype was found in 17% and normal karyotype in 30%. The most frequent immunophenotype was of common B-ALL (43%), and cytogenetic and/or molecular abnormalities were found in 78% of them. We distinguished a relatively high incidence (17%) of mature B-ALL and 60% of them were associated with t(8;14)/C-MYC. We established association of cytogenetic aberrations with immunophenotype only in mature B-ALL. The other immunophenotypes are characterized by genetic heterogeneity and the presence of cytogenetic abnormalities unusual for adult B-ALL - trisomy 8 and t(1;19)(q23;p13).