Traumatic rupture of the pectoralis major muscle with associated thrombosis of the cephalic vein as part of a seat belt injury following a motor vehicle accident: A case report.

Traumatic rupture of the pectoralis major muscle is a rare concomitant injury in polytrauma patients often resulting in delayed diagnosis. We present the case of a young male patient who, among other injuries, suffered a complete rupture of the right-sided pectoralis major muscle at the humeral insertion point following a motor vehicle accident. Duplex sonography demonstrated an associated thrombosis of the cephalic vein, which was treated initially with intravenous heparin, and long-term with low-molecular weight heparin according to current guidelines. An open refixation of the muscle belly at the humeral insertion point was performed two weeks after the initial trauma. Post-operative follow-up presented a good overall outcome in terms of function and aesthetics. The authors emphasize the need of continuous clinical re-evaluation in the treatment of severely injured patients in order not to overlook relevant injuries.

Development and field validation of a Mycoplasma iowae real-time polymerase chain reaction assay.

A Mycoplasma iowae real-time polymerase chain reaction (PCR) assay using primers and probes targeting the 16S rRNA gene was developed and field-validated in this study. The assay specifically identified M. iowae with a detection limit of 80 colony-forming units (cfu) per turkey cloacal swab sample (3.2 cfu per PCR reaction). It was validated by testing 154 field turkey cloacal swab samples in parallel with culture isolation. The diagnostic sensitivity of the PCR was 97.6%, and the specificity was 95.5%. The real-time PCR developed in this study is a rapid, sensitive, and cost-effective alternative to culture isolation for detecting M. iowae from cloacal swab samples.