Diverse groups of fungi are associated with plastics in the surface waters of the Western South Atlantic and the Antarctic Peninsula.

Marine plastic pollution has a range of negative impacts for biota and the colonization of plastics in the marine environment by microorganisms may have significant ecological impacts. However, data on epiplastic organisms, particularly fungi, is still lacking for many ocean regions. To evaluate plastic associated fungi and their geographic distribution, we characterised plastics sampled from surface waters of the western South Atlantic (WSA) and Antarctic Peninsula (AP), using DNA metabarcoding of three molecular markers (ITS2, 18S rRNA V4 and V9 regions). Numerous taxa from eight fungal phyla and a total of 64 orders were detected, including groups that had not yet been described associated with plastics. There was a varied phylogenetic assemblage of predominantly known saprotrophic taxa within the Ascomycota and Basidiomycota. We found a range of marine cosmopolitan genera present on plastics in both locations, i.e., Aspergillus, Cladosporium, Wallemia and a number of taxa unique to each region, as well as a high variation of taxa such as Chytridiomycota and Aphelidomycota between locations. Within these basal fungal groups we identified a number of phylogenetically novel taxa. This is the first description of fungi from the Plastisphere within the Southern Hemisphere, and highlights the need to further investigate the potential impacts of plastic associated fungi on other organisms and marine ecosystems.

Understanding the interactions between cephalopods and marine litter: A research evaluation with identification of gaps and future perspectives.

Litter is known to negatively affect numerous marine organisms, but the extent of such impacts is not well known for several groups, including cephalopods. Considering the ecological, behavioral and economic importance of these animals, we reviewed the types of interactions between cephalopods and litter in the scientific literature, to evaluate impacts and knowledge gaps. We found 30 papers, which included records of microplastic ingestion and the transfer of synthetic microfibers along the food web. The largest number of records involved litter use as shelter, and the common octopus was the most frequent species. At first sight, litter use as shelter could appear to be a potential positive effect, but it is necessary to clarify the implications of this choice and its long-term consequences. Regarding ingestion and trophic transfer, further research is needed to elucidate its occurrence and impacts on cephalopods and their predators, including humans.

Plastisphere composition in a subtropical estuary: Influence of season, incubation time and polymer type on plastic biofouling.

Plastics are abundant artificial substrates in aquatic systems that host a wide variety of organisms (the plastisphere), including potential pathogens and invasive species. Plastisphere communities have many complex, but not well-understood ecological interactions. It is pivotal to investigate how these communities are influenced by the natural fluctuations in aquatic ecosystems, especially in transitional environments such as estuaries. Further study is needed in subtropical regions in the Southern Hemisphere, where plastic pollution is ever increasing. Here we applied DNA-metabarcoding (16S, 18S and ITS-2) as well Scanning Electron Microscopy (SEM) to assess the diversity of the plastisphere in the Patos Lagoon estuary (PLE), South Brazil. Through a one-year in situ colonization experiment, polyethylene (PE) and polypropylene (PP) plates were placed in shallow waters, and sampled after 30 and 90 days within each season. Over 50 taxa including bacteria, fungi and other eukaryotes were found through DNA analysis. Overall, the polymer type did not influence the plastisphere community composition. However, seasonality significantly affected community composition for bacteria, fungi and general eukaryotes. Among the microbiota, we found Acinetobacter sp., Bacillus sp., and Wallemia mellicola that are putative pathogens of aquatic organisms, such as algae, shrimp and fish, including commercial species. In addition, we identified organisms within genera that can potentially degrade hydrocarbons (e.g. Pseudomonas and Cladosporium spp). This study is the first to assess the full diversity and variation of the plastisphere on different polymers within a subtropical Southern Hemisphere estuary, significantly expanding knowledge on plastic pollution and the plastisphere in estuarine regions.

In an octopus's garden in the shade: Underwater image analysis of litter use by benthic octopuses.

Benthic octopuses have been widely documented in artificial shelters for decades, and this use is apparently increasing. Despite any possible positive effects, the use of litter as shelter could have negative implications. In this work, we aimed to elucidate the interactions of octopuses with marine litter, identifying types of interactions and affected species and regions. To achieve this, we obtained 261 underwater images from 'citizen science' records, and identified 8 genera and 24 species of benthic octopuses interacting with litter. Glass objects were present in 41.6% of interactions, and plastic in 24.7%. Asia presented the highest number of images, and most records were from 2018 to 2021. Citizen science provided important evidence on octopus/marine litter interactions, highlighting its value and the need for more investigations on the subject. This information is fundamental to help prevent and mitigate the impacts of litter on octopuses, and identify knowledge gaps that require attention.

Floating plastics and their associated biota in the Western South Atlantic.

The lack of information about plastic pollution in many marine regions hinders firm actions to manage human activities and mitigate their impacts. This study conducted for the first time a quali-quantitative evaluation of floating plastics and their associated biota from coastal and oceanic waters in South Brazil. Plastics were collected using a manta net, and were categorized according to their shape, size, malleability and polymer composition. Multi-marker DNA metabarcoding (16S, and 18S V4 and V9 rRNA regions) was performed to identify prokaryotes and eukaryotes associated to plastics. We found 371 likely plastic particles of several sizes, shapes and polymers, and the average concentration of plastics at the region was 4461 items.km-2 (SD ± 3914). Microplastics (0.5 - 5 mm) were dominant in most sampling stations, with fragments and lines representing the most common shapes. Diverse groups of prokaryotes (20 bacteria phyla) and eukaryotes (41 groups) were associated with plastics. Both the community composition and richness of epiplastic organisms were highly variable between individual plastics but, in general, were not influenced by plastic categories. Organisms with potential pathogenicity (e.g. Vibrio species. and Alexandrium tamarense), as well as potential plastic degraders (e.g. Ralstonia, Pseudomonas, and Alcanivorax species), were found. The information generated here is pivotal to support strategies to prevent the input and mitigate the impacts of plastics and their associated organisms on marine environments.

Ingestion of plastic debris by commercially important marine fish in southeast-south Brazil.

Rising concentrations of plastics in the oceans are leading to increasing negative interactions with marine biota, including ingestion by endangered and/or economically important seafood species such as fish. In this paper, we visually evaluated plastic debris ingestion by 965 specimens of eight commercially exploited fish species from different marine habitats off the southeast-south coast of Brazil. All species ingested plastics, with pelagic animals having higher amounts, frequency of occurrence, diversity and sizes of ingested items than demersal-pelagic and demersal animals. Highest frequency of occurrence (FO%) of plastic ingestion (25.8%) was observed for the pelagic skipjack tuna Katsuwonus pelamis (Scombridae), and lowest (5%) for the demersal bluewing searobin Prionotus punctatus (Triglidae). Microplastics predominated in all species, and fibers/lines and fragments were the main items found, possibly derived from fishing materials. The most abundant plastic colors were transparent, black and blue, and the most common polymers were polyamide and polyurethane. With the available data, no relationship between the size of the individuals and amount of ingested plastics was observed. Considering the negative impacts of plastic ingestion on marine fish, and potentially on human health due to their consumption, understanding ingestion patterns is critical for better evaluating their origin and possible causes, and consequently for helping define prevention strategies for this problem.

Ingestion of plastic marine litter by sea turtles in southern Brazil: abundance, characteristics and potential selectivity.

The ingestion of plastic marine litter (PML) by sea turtles is widespread and concerning, and the five species that occur in the southwestern Atlantic - green, loggerhead, olive ridley, leatherback and hawksbill - are vulnerable to this pollution. Here, we quantified and characterized PML ingested by these species in southern Brazil, and observed PML ingestion in 49 of 86 sampled individuals (~57.0%). Green turtles presented the highest rates and variety of ingested plastics, and such ingestion has been high at least since 1997. Omnivorous turtles presented higher PML ingestion than carnivorous ones. Loggerheads displayed a negative correlation between body size and number of ingested items. Green turtles ingested mostly flexible transparent and flexible/hard white plastics; loggerheads ate mainly flexible, hard and foam fragments, in white and black/brown colors. These results help us better understand PML ingestion by sea turtles, highlighting the seriousness of this threat and providing information for prevention and mitigation strategies.

Plastics in sea surface waters around the Antarctic Peninsula.

Although marine plastic pollution has been the focus of several studies, there are still many gaps in our understanding of the concentrations, characteristics and impacts of plastics in the oceans. This study aimed to quantify and characterize plastic debris in oceanic surface waters of the Antarctic Peninsula. Sampling was done through surface trawls, and mean debris concentration was estimated at 1,794 items.km-2 with an average weight of 27.8 g.km-2. No statistical difference was found between the amount of mesoplastics (46%) and microplastics (54%). We found hard and flexible fragments, spheres and lines, in nine colors, composed mostly of polyurethane, polyamide, and polyethylene. An oceanographic dispersal model showed that, for at least seven years, sampled plastics likely did not originate from latitudes lower than 58°S. Analysis of epiplastic community diversity revealed bacteria, microalgae, and invertebrate groups adhered to debris. Paint fragments were present at all sampling stations and were approximately 30 times more abundant than plastics. Although paint particles were not included in plastic concentration estimates, we highlight that they could have similar impacts as marine plastics. We call for urgent action to avoid and mitigate plastic and paint fragment inputs to the Southern Ocean.

Genetic structure and natal origins of immature hawksbill turtles (Eretmochelys imbricata) in Brazilian waters.

Understanding the connections between sea turtle populations is fundamental for their effective conservation. Brazil hosts important hawksbill feeding areas, but few studies have focused on how they connect with nesting populations in the Atlantic. Here, we (1) characterized mitochondrial DNA control region haplotypes of immature hawksbills feeding along the coast of Brazil (five areas ranging from equatorial to temperate latitudes, 157 skin samples), (2) analyzed genetic structure among Atlantic hawksbill feeding populations, and (3) inferred natal origins of hawksbills in Brazilian waters using genetic, oceanographic, and population size information. We report ten haplotypes for the sampled Brazilian sites, most of which were previously observed at other Atlantic feeding grounds and rookeries. Genetic profiles of Brazilian feeding areas were significantly different from those in other regions (Caribbean and Africa), and a significant structure was observed between Brazilian feeding grounds grouped into areas influenced by the South Equatorial/North Brazil Current and those influenced by the Brazil Current. Our genetic analysis estimates that the studied Brazilian feeding aggregations are mostly composed of animals originating from the domestic rookeries Bahia and Pipa, but some contributions from African and Caribbean rookeries were also observed. Oceanographic data corroborated the local origins, but showed higher connection with West Africa and none with the Caribbean. High correlation was observed between origins estimated through genetics/rookery size and oceanographic/rookery size data, demonstrating that ocean currents and population sizes influence haplotype distribution of Brazil's hawksbill populations. The information presented here highlights the importance of national conservation strategies and international cooperation for the recovery of endangered hawksbill turtle populations.

Hawksbill × loggerhead sea turtle hybrids at Bahia, Brazil: where do their offspring go?

Hybridization between hawksbill (Eretmochelys imbricata) and loggerhead (Caretta caretta) breeding groups is unusually common in Bahia state, Brazil. Such hybridization is possible because hawksbill and loggerhead nesting activities overlap temporally and spatially along the coast of this state. Nevertheless, the destinations of their offspring are not yet known. This study is the first to identify immature hawksbill × loggerhead hybrids (n = 4) from this rookery by analyzing the mitochondrial DNA (mtDNA) of 157 immature turtles morphologically identified as hawksbills. We also compare for the first time modeled dispersal patterns of hawksbill, loggerhead, and hybrid offspring considering hatching season and oceanic phase duration of turtles. Particle movements varied according to season, with a higher proportion of particles dispersing southwards throughout loggerhead and hybrid hatching seasons, and northwards during hawksbill season. Hybrids from Bahia were not present in important hawksbill feeding grounds of Brazil, being detected only at areas more common for loggerheads. The genetic and oceanographic findings of this work indicate that these immature hybrids, which are morphologically similar to hawksbills, could be adopting behavioral traits typical of loggerheads, such as feeding in temperate waters of the western South Atlantic. Understanding the distribution, ecology, and migrations of these hybrids is essential for the development of adequate conservation and management plans.