Phenotypic and Genetic Characterization of Botrytis cinerea Population from Kiwifruit in Sichuan Province, China.

Botrytis cinerea (anamorph of Botryotinia fuckeliana) causes gray mold on numerous plants, including kiwifruit. The primary aim of this study was to investigate the phenotypic and genetic characteristics of the Botrytis cinerea population from kiwifruit in Sichuan Province, China. In all, 176 isolates were collected from kiwifruit orchards from eight geographic regions in Sichuan. All isolates were identified as B. cinerea sensu stricto based on the combined datasets, including morphological criteria, determination of the Bc-hch allele, and phylogenetic analysis of the genes RPB2, G3PDH, and HSP60. Three colony types (i.e., sclerotial, mycelial, and conidial) were observed on potato dextrose agar after 2 weeks, with sclerotial isolates, the predominant category, accounting for 40.91%. No obvious differences in microscopic characteristics were observed among the three types. Three genotypes of transposable elements were identified in the B. cinerea population: boty, flipper, and transposa types. The most prevalent genotype from different geographic populations of B. cinerea was transposa; in contrast, the flipper genotype accounted for only 3.98% of the total population, whereas the vacuma genotype was absent. According to MAT locus amplification, 87 and 89 isolates are MAT1-1 and MAT1-2 type, respectively, and the two mating types were found to be balanced overall in the population. Forty-eight representative isolates were all able to cause gray mold to some extent, and disease severities were significantly different between the cultivars Hongyang and Hort16A (P < 0.01). Disease severity was significantly greater on young leaves than on mature leaves (P < 0.01). No significant relationship was found between pathogenicity and geographical region, colony type, or transposon distribution. The results obtained in the present study suggest a relatively uniform species diversity of Botrytis but rich phenotypic and genetic differentiation within the B. cinerea population on kiwifruit in China. Utilizing resistant cultivars and rain-shelter cultivation instead of fungicides may be an effective approach to delaying pathogen variability.

Treatment of pertrochanteric fractures with a proximal femur locking compression plate.

BACKGROUND: Pertrochanteric femoral fractures are one of the most common fractures in old patients. However, fixing pertrochanteric fractures properly is clinically challenging. There are also no routine treatments for this fracture. Here, we report the clinical trial of pertrochanteric fracture treatment with a proximal femur locking compression plate (PFLCP). By recording and analysing the radiographic and clinical results from patients treated with PFLCP, we found that PFLCP could provide three-dimensional fixation mechanical advantages compared with conventional treatments, even in the case of unstable fractures in the osteoporotic bone. METHODS: The report included a total of 110 patients (72 females and 38 males) with pertrochanteric femoral fractures who were subjected to PFLCP treatment. The mean age of the patients was 75 (48-93) years. Pertrochanteric fracture includes both intertrochanteric and subtrochanteric femoral fractures: intertrochanteric fractures were classified according to Jensen (1980), whereas subtrochanteric fractures were classified according to Zickel (1980). Detailed clinical conditions of all patients, including blood loss, drainage and length of incision, were recorded individually. The duration of image intensification was also monitored. Patients were revisited at 6 weeks, 3 months, 6 months and 1 year after the operation. The progress of healing, as well as the occurrence of complications, was recorded. RESULTS: Amongst the 110 patients, 108 (98%) were available for follow-up check-up at 6 weeks, 104 (95%) at 3 months, 100 (91%) at 6 months and 94 (85%) at 1 year. The other patients were lost to follow-up because of death. The union rate was 95% (99/104), 98% (98/100) and 100% (94/94) at the 3-month, 6-month and 1-year period during the follow-up check-up, respectively. The patients healed satisfactorily and had no complications, such as cut-out in most cases. However, there was one case of breakage of the implant and one case of non-union at the 3-month period during the follow-up check-up. Amongst all patients, 77 cases were successfully reduced with traction on a fracture table under fluoroscopy; the others were opened to correct the displacement. The average operation time was 35.5 min, and the mean bleeding amount was 150 ml (including operative blood loss and wound drainage). The mean image intensifier time was 5 min and the mean length of incision was 9 cm. CONCLUSION: The PFLCP can be a feasible alternative to the treatment of pertrochanteric fractures. Treatment with a PFLCP can provide good-to-excellent healing for pertrochanteric fractures, with a limited occurrence of complications.