Tagitinin A from Tithonia diversifolia provides resistance to tomato spotted wilt orthotospovirus by inducing systemic resistance.

Tomato spotted wilt orthotospovirus (TSWV) causes devastating losses to agronomic and ornamental crops worldwide. Currently, there is no effective strategy to control this disease. Use of biotic inducers to enhance plant resistance to viruses maybe an effective approach. Our previous study indicated that Tagitinin A (Tag A) has a high curative and protective effect against TSWV. However, the underlying molecular mechanism of Tag A-mediated antiviral activity remains unknown. In this study, Tag A reduced the expression of the NSs, NSm genes was very low in untreated leaves following TSWV infection. In addition, the expression of all TSWV genes in the inoculated and systemic leaves was inhibited in the protective assay, and with an inhibition rate of more than 85% in systemic leaves. Tag A increased phenylalanine ammonia-lyase (PAL) activity in the curative and protective assays. The concentrations of jasmonic acid (JA) and jasmonic acid -isoleucine (JA-Ile) and the expression of its key gene NtCOI1 in Tag A-treated and systemic leaves of treated plants were significantly higher than those of the control plant. Furthermore, Tag A-induced resistance to TSWV could be eliminated by VIGS-mediated silencing of the NtCOI1 gene. These indicated that Tag A acts against TSWV by activating the JA defense signaling pathway.

Leaving no one behind: how women seize control of wheat-maize technologies in Bangladesh.

Bangladesh is strongly committed to the "leave no one behind" principle of the UN's Sustainable Development Goals. However, social norms and institutional biases in agricultural organisations can prevent indigenous peoples and women from participating in wheat-maize innovation processes, as they rarely meet the requisite criteria: sufficient land, social capital or formal education. The GENNOVATE (Enabling Gender Equality in Agricultural and Environmental Innovation) research initiative in Bangladesh shows that indigenous Santal women are obtaining access to and benefiting from wheat-maize innovations, enabling low-income Muslim women to benefit as well.

Le Bangladesh est fortement attaché au principe que personne ne soit « laissé pour compte ¼, l'un des Objectifs de développement durable de l'ONU. Cependant, certaines normes sociales et biais institutionnels au sein de organisations agricoles empêchent parfois les Autochtones et les femmes de prendre part à des processus d'innovations relatives à la culture du maïs et du blé, car ils répondent rarement aux critères requis : terres suffisantes, capital social ou éducation formelle. Au Bangladesh, l'initiative de recherche GENNOVATE (Promouvoir l'égalité de genre dans l'innovation agricole et environnementale) montre que les femmes autochtones du Santal obtiennent accès aux « innovations blé-maïs ¼ et en bénéficient, ce qui permet également aux femmes musulmanes à faible revenu d'en bénéficier.

Population-based survey of the prevalence and types of glaucoma in Bangladesh.

BACKGROUND: To delineate the epidemiological landscape of glaucoma using a population-based sample representative of Bangladesh. METHODS: Using multistage stratified cluster random sampling, households were selected to identify individuals ≥35 years across all 8 divisions of Bangladesh. Sampling frames were derived from the 2011 national census. Fifty-eight study examination sites were set up for comprehensive eye evaluations, including intraocular pressure, gonioscopy and visual field testing when indicated. International Society for Geographic and Epidemiological Ophthalmology definitions were used to define glaucoma and glaucoma suspect cases. RESULTS: One hundred forty clusters (89 rural and 51 urban) were randomly selected, and 13 791 residential households were visited. We invited 17 002 individuals ≥35 years for on-site examination, of which 12 000 (71%) complied, with a male-to-female ratio of 1:1. The prevalence of glaucoma was 3.2% (95% CI 2.79% to 3.64%), and glaucoma suspect was 10.1% (95% CI 9.05% to 11.12%). The majority (78%) had primary open-angle glaucoma (POAG), while angle closure was seen in 16%. Of the POAG, 83% (n=251) were normal-tension glaucoma. Multivariable logistic regression showed increasing age (OR=1.01 for every 5-year increment, 95% CI 1 to 1.01) and male gender (OR=1.43, 95% CI 1.15 to 1.77) to be associated with an increased risk of glaucoma. CONCLUSIONS: The prevalence of glaucoma in Bangladesh is 3.2% in ≥35-year-old individuals with older men most at risk. Extrapolating the results, we estimate about 2 million patients with glaucoma. Though normal-tension variety was the most common type, caution should be exercised in generalising these results to other populations.

Green logistics and environment, economic growth in the context of the Belt and Road Initiative.

Infrastructure development has been a priority area since the Belt and Road Initiative (BRI) was proposed. This paper uses the generalized method of moments (GMM) to analyze the relationship between the green logistics, environment and economic growth based on panel data of countries along the BRI from 2007 to 2018. It is found that fossil fuels are the core of logistics operation activities, and the more fossil fuels are used, the more detrimental to the sustainable development of the environment. "The green logistics is negatively correlated with fossil fuel energy consumption and carbon emissions per capita in countries along the Belt and Road. At the same time, the green logistics can also bring more export opportunities for these countries and increase the national income per capita. The development of the green logistics is of great significance to the countries' environment and sustainable economic development along the Belt and Road.

Diagnostic efficacy of Brucella abortus strain RB51 in experimentally inoculated Sprague-Dawley rats using western blot assay.

BACKGROUND: To investigate the diagnosis and efficacy of Brucella abortus strain RB51 (SRB51) in experimentally inoculated Sprague-Dawley (SD) rat using western blot assay. METHODOLOGY: Female SD rats were orally administered with 1.0 x 10(7) colony forming unit (cfu) suspension of SRB51 and half of these SD rats were challenged at 4 weeks post inoculation with 1.0 x 10(9) cfu suspension of B. abortus biotype 1 isolated in South Korea. Sera of SD rats were monitored at regular intervals by western blot assay using whole cell antigen of B. abortus strain 1119-3 (S1119-3). The bacteriological examination of blood and clinical examination of the rats were also performed. RESULTS: There were several bands at 120, 70, 45, 30, 20 kDa and clear specific bands were found after vaccination (20, 70 kDa) and challenge (15, 20, 45, 70, 120 kDa). The highest immune response was observed in sera 4 weeks post SRB51 vaccination. SRB51 was recovered from the blood of all of SRB51 inoculated rats until one week post vaccination and there were no clinical signs in that inoculated rats. CONCLUSIONS: It is concluded that the SRB51 elicits antigen specific immunity in SD rats based on western blot assay.