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1.
Microb Pathog ; 158: 105057, 2021 Sep.
Artigo em Inglês | MEDLINE | ID: mdl-34153419

RESUMO

The current pandemic due to the fast spreading of SARS-CoV-2 infection has caused severe impairment in health, social, economic, scientific, and medical sectors across the globe. Owing to the not so well understood mechanism of disease pathogenesis in terms of variations in immune responses, there remains obscure why some of the patients who are infected by the novel SARS-CoV-2 develop an unpredictable clinical course that rapidly causes severe and deadly complications/manifestations. Currently, several assays are available for the confirmation of SARS-CoV-2 infection at the point of care. However, none of these assays can predict the severity of the COVID-19 disease. Thus, the identification of a prognostic biomarker that forecasts the condition of SARS-CoV-2 patients to develop a severe form of the disease could enable the clinicians for more efficient patient triage and treatment. In this regard, the present review describes the role of selected biomolecules that are crucially involved in the immune-pathogenesis of SARS-CoV-2 infection such as hyper-immune responsiveness, bradykinin storm and vascular leakage assuming these may serve as an effective prognostic biomarker in COVID-19 to understand the outcome of the disease. Based on the review, we also propose the development of a cost-effective SERS-based prognostic biosensor for the detection and quantification of biomolecules for use as a point-of-care system during a disease outbreak.


Assuntos
COVID-19 , SARS-CoV-2 , Biomarcadores , Humanos , Pandemias
2.
Br J Cancer ; 116(2): 227-236, 2017 Jan 17.
Artigo em Inglês | MEDLINE | ID: mdl-28006818

RESUMO

BACKGROUND: Recent evidence suggests that bone-related parameters are the main prognostic factors for overall survival in advanced prostate cancer (PCa), with elevated circulating levels of alkaline phosphatase (ALP) thought to reflect the dysregulated bone formation accompanying distant metastases. We have identified that PCa cells express ALPL, the gene that encodes for tissue nonspecific ALP, and hypothesised that tumour-derived ALPL may contribute to disease progression. METHODS: Functional effects of ALPL inhibition were investigated in metastatic PCa cell lines. ALPL gene expression was analysed from published PCa data sets, and correlated with disease-free survival and metastasis. RESULTS: ALPL expression was increased in PCa cells from metastatic sites. A reduction in tumour-derived ALPL expression or ALP activity increased cell death, mesenchymal-to-epithelial transition and reduced migration. Alkaline phosphatase activity was decreased by the EMT repressor Snail. In men with PCa, tumour-derived ALPL correlated with EMT markers, and high ALPL expression was associated with a significant reduction in disease-free survival. CONCLUSIONS: Our studies reveal the function of tumour-derived ALPL in regulating cell death and epithelial plasticity, and demonstrate a strong association between ALPL expression in PCa cells and metastasis or disease-free survival, thus identifying tumour-derived ALPL as a major contributor to the pathogenesis of PCa progression.


Assuntos
Fosfatase Alcalina/fisiologia , Proliferação de Células/genética , Transição Epitelial-Mesenquimal/genética , Neoplasias da Próstata/patologia , Neoplasias da Próstata/terapia , Fosfatase Alcalina/genética , Animais , Morte Celular/genética , Movimento Celular/genética , Células Cultivadas , Progressão da Doença , Intervalo Livre de Doença , Células HEK293 , Humanos , Masculino , Camundongos , Metástase Neoplásica , Neoplasias da Próstata/genética
3.
J Biol Regul Homeost Agents ; 31(1): 77-80, 2017.
Artigo em Inglês | MEDLINE | ID: mdl-28337873

RESUMO

Cyclosporine-A induces gingival overgrowth with disturbance in the homeostasis of cells and connective tissue proteins. Human gingival fibroblasts were cultured with cyclosporine A, and the expression of two vital endoplasmic stress markers and two prime matrix proteins (connective tissue growth factor (CTGF and periostin) were assessed by RT-PCR. We found that expression of Glucose-Regulated Protein 78 (GRP78/BIP) and CCAAT/enhancer binding protein, C/EBP homologous protein (CHOP) were significantly increased, along with CTGF and periostin, suggesting a role for these factors in gingival overgrowth.


Assuntos
Moléculas de Adesão Celular/genética , Fator de Crescimento do Tecido Conjuntivo/genética , Ciclosporina/efeitos adversos , Estresse do Retículo Endoplasmático/efeitos dos fármacos , Proteínas de Choque Térmico/genética , Imunossupressores/efeitos adversos , Fator de Transcrição CHOP/genética , Biomarcadores/metabolismo , Moléculas de Adesão Celular/agonistas , Moléculas de Adesão Celular/metabolismo , Fator de Crescimento do Tecido Conjuntivo/agonistas , Fator de Crescimento do Tecido Conjuntivo/metabolismo , Retículo Endoplasmático/efeitos dos fármacos , Retículo Endoplasmático/metabolismo , Chaperona BiP do Retículo Endoplasmático , Estresse do Retículo Endoplasmático/genética , Fibroblastos/citologia , Fibroblastos/efeitos dos fármacos , Fibroblastos/metabolismo , Regulação da Expressão Gênica , Gengiva/citologia , Gengiva/efeitos dos fármacos , Gengiva/metabolismo , Proteínas de Choque Térmico/agonistas , Proteínas de Choque Térmico/metabolismo , Humanos , Cultura Primária de Células , Transdução de Sinais , Fator de Transcrição CHOP/agonistas , Fator de Transcrição CHOP/metabolismo
4.
Tsitol Genet ; 49(6): 50-60, 2015.
Artigo em Inglês | MEDLINE | ID: mdl-26841494

RESUMO

Karyomorphological studies have been carried out in nine species and five varieties of the genus Cucumis representing Indian gene pool. The present investigations reveal the occurrence of two somatic chromosome numbers 2n = 14, 24 in the genus. C. ritchiei and C. indicus the two new species, were found to be having somatic chromosome numbers of 2n = 24 and 2n = 20 respectively. The wild species viz. C. hystrix, C. setosus, C. prophetarum, C. dipsaceus, C. indicus have very less number of median-centromeric chromosomes, high asymmetry indices, while melon groups have intermediate number of median -centromeric chromosomes. C. sativus, C. callosus, C. ritchiei show lesser number median-cen-tromeric chromosomes and very less asymmetry indices. The importance of karyotypic variation with respect to speciation within the genus Cucumis have been discussed.


Assuntos
Estruturas Cromossômicas , Cromossomos de Plantas/genética , Cucumis/genética , Mapeamento Cromossômico , Cromossomos de Plantas/diagnóstico por imagem , Cucumis/citologia , Cucumis/crescimento & desenvolvimento , Índia , Cariotipagem , Especificidade da Espécie , Ultrassonografia
5.
Hum Cell ; 35(6): 1633-1639, 2022 Nov.
Artigo em Inglês | MEDLINE | ID: mdl-36068397

RESUMO

Endothelial dysfunction is one of the key cornerstone complications of emerging and re-emerging viruses which lead to vascular leakage and a high mortality rate. The mechanism that regulates the origin of endothelial dysregulation is not completely elucidated. Currently, there are no potential pharmacological treatments and curable management for such diseases. In this sense, mesenchymal stromal/stem cells (MSCs) has been emerging to be a promising therapeutic strategy in restoring endothelial barrier function in various lung disease, including ALI and ARDS. The mechanism of the role of MSCs in restoring endothelial integrity among single-strand RNA (ssRNA) viruses that target endothelial cells remains elusive. Thus, we have discussed the therapeutic role of MSCs in restoring vascular integrity by (i) inhibiting the metalloprotease activity thereby preventing the cleavage of tight junction proteins, which are essential for maintaining membrane integrity (ii) possessing antioxidant properties which neutralize the excessive ROS production due to virus infection and its associated hyper host immune response (iii) modulating micro RNAs that regulate the endothelial activation and its integrity by downregulating the inflammatory response during ssRNA infection.


Assuntos
Transplante de Células-Tronco Mesenquimais , Células-Tronco Mesenquimais , Viroses , Antioxidantes/metabolismo , Células Endoteliais/metabolismo , Humanos , Células-Tronco Mesenquimais/fisiologia , Metaloproteases/metabolismo , RNA , Espécies Reativas de Oxigênio/metabolismo , Proteínas de Junções Íntimas/metabolismo , Viroses/metabolismo
6.
Biochimie ; 201: 139-147, 2022 Oct.
Artigo em Inglês | MEDLINE | ID: mdl-35724946

RESUMO

SARS-CoV-2 uses membrane bound Angiotensin-Converting Enzyme 2 (ACE2) as a key host receptor for its entry. However, inconsistent results are available in terms of shedding of membrane ACE2 and circulating levels of soluble ACE2 during SARS-CoV-2. To ascertain soluble ACE2 as an effective biomarker for the prediction of COVID-19 outcome, in the present study, we investigated the levels of plasma ACE2 during the early phase of infection in COVID-19 patients. The study involved a total of 42 COVID-19 patients along with 10 healthy controls. Plasma levels of ACE2 was determined using ELISA at the time of admission and on day 7 post admission. The association of sACE2 with D-dimer a marker for hyper-coagulation was performed using a dependence test. Compared to healthy controls, SARS-CoV-2 cases has shown a huge increase in the sACE2 at the time of admission. During the course of infection, we found a significant increase (P ≤ 0.001) in sACE2 in severe cases compared to moderate. There was a strong increase in sACE2 in cases with hypertension and diabetes mellitus. Interestingly, a strong positive correlation (P ≤ 0.001) was obtained between sACE2 and D-dimer. Thus, an excessive shedding of ACE2 during the early phase is a common phenomenon in severe form of the SARS-CoV-2. Along with D-dimer, the sACE2 levels could serve as a clinical biomarker for the prediction of disease outcome. However further studies are needed to ascertain its role in host-virus interplay.


Assuntos
Enzima de Conversão de Angiotensina 2 , COVID-19 , Biomarcadores , Humanos , Peptidil Dipeptidase A/metabolismo , SARS-CoV-2 , Índice de Gravidade de Doença
7.
Plants (Basel) ; 11(19)2022 Sep 27.
Artigo em Inglês | MEDLINE | ID: mdl-36235403

RESUMO

Genome- or gene-editing (abbreviated here as 'GEd') presents great opportunities for crop improvement. This is especially so for the countries in the Asia-Pacific region, which is home to more than half of the world's growing population. A brief description of the science of gene-editing is provided with examples of GEd products. For the benefits of GEd technologies to be realized, international policy and regulatory environments must be clarified, otherwise non-tariff trade barriers will result. The status of regulations that relate to GEd crop products in Asian countries and Australasia are described, together with relevant definitions and responsible regulatory bodies. The regulatory landscape is changing rapidly: in some countries, the regulations are clear, in others they are developing, and some countries have yet to develop appropriate policies. There is clearly a need for the harmonization or alignment of GEd regulations in the region: this will promote the path-to-market and enable the benefits of GEd technologies to reach the end-users.

8.
J Postgrad Med ; 57(1): 9-15, 2011.
Artigo em Inglês | MEDLINE | ID: mdl-21206128

RESUMO

BACKGROUND: Cardiovascular diseases are one of the leading causes of death in India. There is high prevalence of cardiovascular risk factors in urban Tamil Nadu. There are limited data on the prevalence of behavioral risk factors and overweight in rural Tamil Nadu. AIM: We estimated prevalence of behavioral risk factors, overweight and central obesity in a rural population in Tamil Nadu, India. SETTING AND DESIGN: We conducted a cross-sectional survey in 11 villages in Kancheepuram/Thiruvallur districts, Tamil Nadu. MATERIALS AND METHODS: Study population included 10,500 subjects aged 25-64 years. We collected data on behavioral risk factors and anthropometric measurements. Body mass index (BMI) was categorized using the classification recommended for Asians. Central obesity was defined as waist circumference ≥90 cm for men and ≥80 cm for women. We computed proportions for all risk factors and used trend chi-square to examine trend. RESULTS: Among the 10,500 subjects, 4927 (47%) were males. Among males, 1852 (37.6%) were current smokers and 3073 (62.4%) were current alcohol users. Among females, 840 (15.1%) were smokeless tobacco users. BMI was ≥23.0 kg/m 2 for 1618 (32.8%) males and 2126 (38.2%) females. 867 (17.6%) males and 1323 (23.7%) females were centrally obese. Most commonly used edible oil was palm oil followed by sunflower oil and groundnut oil. CONCLUSION: We observed high prevalence of tobacco use, alcohol use and central obesity in the rural population in Tamil Nadu. There is need for health promotion programs to encourage adoption of healthy lifestyle and policy interventions to create enabling environment.


Assuntos
Alcoolismo/epidemiologia , Sobrepeso/epidemiologia , Tabagismo/epidemiologia , Adulto , Distribuição por Idade , Índice de Massa Corporal , Doenças Cardiovasculares/epidemiologia , Estudos Transversais , Feminino , Humanos , Índia/epidemiologia , Estilo de Vida , Masculino , Pessoa de Meia-Idade , Prevalência , Fatores de Risco , População Rural/estatística & dados numéricos , Distribuição por Sexo , Fumar/efeitos adversos , Fumar/epidemiologia
9.
Front Public Health ; 9: 673536, 2021.
Artigo em Inglês | MEDLINE | ID: mdl-34178928

RESUMO

The key challenges to any health care setup during emergency situations, such as that of the COVID-19 pandemic would be to rapidly address hospital preparedness and response tailored to the local population, societal influences, political factors within the existing infrastructure, and workforce. Second, to adopt and moderate policies, standard operating procedures (SOPs) and guidelines issued by national and international agencies, such as WHO, CDC, and the Indian Council for Medical Research (ICMR) were tailor-made to the local conditions of the hospital and community. In this publication, we have discussed the challenges and experiences in preparation and responses to the ongoing COVID-19 pandemic at a tertiary teaching hospital situated at a suburban locale in a small union territory. Puducherry is located in the South Eastern Coromandel Coast of India. The core processes, such as hospital preparedness, adoption, and amendments to SOPs based on dynamic changes in guidelines released by the central and local government, training given to health care workers, setting up the in-house diagnostic facility, surge capacity, management of supplies during the lockdown, infection prevention, and control and patient care are discussed. We have also reinforced our experiences in translating COVID-related opportunities for research and innovation in the form of awards and research proposals for the faculty and students of our institute. The lessons learned in terms of strength and limitations on the ground level of public health during this process is worth sharing as it would provide guidance in preparing the health care setups for pre- and post-pandemic.


Assuntos
COVID-19 , Pandemias , Controle de Doenças Transmissíveis , Hospitais de Ensino , Humanos , Índia/epidemiologia , SARS-CoV-2 , Atenção Terciária à Saúde
10.
Indian J Exp Biol ; 47(2): 140-6, 2009 Feb.
Artigo em Inglês | MEDLINE | ID: mdl-19374170

RESUMO

A protocol has been devised for enhanced in vitro regeneration of critically endangered Mantisia spathulata Schult. and Mantisia wengeri Fischer. Highest Bud Forming Capacity (BFC) of 6.10 +/- 0.55 with an average of 19.93 +/- 3.19 roots was obtained for M. spathulata within 5-6 weeks in Murashige and Skoogs (MS) medium supplemented with a combination of 10.0 microM of N6-benzyladenine (BA) and 2.5 microM of alpha-naphtalene acetic acid (NAA). For M. wengeri, BFC of 7.82 +/- 0.73 and 20.86 +/- 1.65 roots was achieved in MS media supplemented with a combination of 5.0 microM BA and 2.5 microM of NAA RAPD markers were used to evaluate the genetic stability of in vitro raised hardened plantlets. Similarity coefficient among the regenerated plants ranged between 0.85-0.98 for M. spathulata and 0.83-0.98 for M. wengeri. Maximum of 88 and 90% genetic similarity were obtained between in vitro raised hardened plantlets and mother stock of M. spathulata and M. wengeri, respectively through RAPD analysis. The hardened plantlets after RAPD analysis on being transferred to soil of experimental garden showed no marked phenotypic variations in vegetative or floral characteristics.


Assuntos
Agricultura/métodos , Técnica de Amplificação ao Acaso de DNA Polimórfico/métodos , Regeneração , Rizoma/fisiologia , Zingiberaceae/fisiologia , DNA de Plantas/análise , Regulação da Expressão Gênica de Plantas , Marcadores Genéticos , Rizoma/anatomia & histologia , Zingiberaceae/anatomia & histologia
12.
Genet Mol Res ; 5(4): 816-27, 2006 Dec 12.
Artigo em Inglês | MEDLINE | ID: mdl-17183489

RESUMO

In order to survive at high temperatures, thermophilic prokaryotes (Archaea and Eubacteria) adopt different strategies. Among several important contributing factors for stability of proteins are CG-rich codons, the ratio of charged amino acids compared to uncharged amino acids, ionic interactions, amino acid preferences and their distribution, post-translational modifications, and solute accumulation. However, these factors may differ from taxon to taxon, both within and between species depending upon the composition of proteins found in these organisms. This is exemplified in the case of differences in strategies adopted by soluble proteins and membrane proteins. Therefore, it appears that no single factor or combination of factors together can be universally attributed to the provision of thermal stability in proteins.


Assuntos
Archaea/metabolismo , Proteínas Arqueais/metabolismo , Bactérias/metabolismo , Proteínas de Bactérias/metabolismo , Temperatura Alta , Termodinâmica , Adaptação Biológica , Aminoácidos/metabolismo , Archaea/genética , Bactérias/genética , Relação Estrutura-Atividade
13.
Waste Manag ; 26(5): 504-8, 2006.
Artigo em Inglês | MEDLINE | ID: mdl-15975784

RESUMO

Resource recovery from two metallurgical residues by flotation was investigated applying an electrostatic model to select initial conditions. The first, a sulphation roast/water leach residue, was processed to float lead sulphate, comparing dodecylamine and xanthate collectors. From the second, a neutralization residue, gypsum, was recovered by reverse flotation of ferric hydroxide, comparing oleate and sulphonate collectors. In both cases, further upgrading by acid leaching was considered.


Assuntos
Sulfato de Cálcio/isolamento & purificação , Resíduos Industriais , Chumbo/isolamento & purificação , Modelos Teóricos , Eliminação de Resíduos/métodos , Aminas/química , Cálcio/análise , Sulfato de Cálcio/química , Compostos Férricos/química , Ferro/análise , Chumbo/análise , Chumbo/química , Metalurgia , Eletricidade Estática , Ácidos Sulfúricos/química , Zinco/análise
14.
Gynecol Oncol Rep ; 12: 52-4, 2015 Apr.
Artigo em Inglês | MEDLINE | ID: mdl-26076159

RESUMO

•We report a rare case of solitary fibrous tumor of vulva.•The lesion is usually benign.•Needs workup for malignant features and other close differentials.•Early aggressive resection ensuring clear margins is recommended.•Tendency for recurrence warrants long term follow-up.

15.
Biotechnol Adv ; 20(2): 101-53, 2002 May.
Artigo em Inglês | MEDLINE | ID: mdl-14538059

RESUMO

This review deals with the production of high-value secondary metabolites including pharmaceuticals and food additives through plant cell cultures, shoot cultures, root cultures and transgenic roots obtained through biotechnological means. Plant cell and transgenic hairy root cultures are promising potential alternative sources for the production of high-value secondary metabolites of industrial importance. Recent developments in transgenic research have opened up the possibility of the metabolic engineering of biosynthetic pathways to produce high-value secondary metabolites. The production of the pungent food additive capsaicin, the natural colour anthocyanin and the natural flavour vanillin is described in detail.

16.
Am J Cardiol ; 64(5): 363-8, 1989 Aug 01.
Artigo em Inglês | MEDLINE | ID: mdl-2502903

RESUMO

Indium-111 platelet scintigraphy accurately detects acute deep venous thrombosis in asymptomatic high-risk patients and may be used as a surveillance test. However, its value in symptomatic patients and its accuracy early after platelet injection are not satisfactorily established. The latter is important for timely institution of therapy. Accordingly, 65 patients (67 limbs) with suspected deep venous thrombosis (symptom duration 8 +/- 10 days, mean +/- standard deviation) were prospectively studied with platelet scintigraphy and contrast venography. Platelets were labeled with 405 +/- 101 mCi indium-111 oxine. The labeling efficiency was 80 +/- 10%. All images were acquired within 120 minutes after intravenous administration of the platelet suspension. Both platelet scintigraphy and venography were interpreted independently by 2 blinded observers (for each technique). Five separate analyses were performed. Each scintigraphic reader was compared to each venographic reader. A fifth analysis--consisting of readings with blinded agreement of both readings of the platelet scans and both readings of the venograms--was performed. Interobserver agreement was 92% for venography and 79% for scintigraphy. Excluding anticoagulated patients, the sensitivity of platelet scintigraphy was between 38 and 46% and the specificity was between 92 and 100%. Thus, early imaging of labeled platelets for the diagnosis of symptomatic deep venous thrombosis carries a high specificity but a much lower sensitivity. It is speculated that the low sensitivity is related to the inactivity of the thrombus. This may suggest that early imaging will only be useful in patients whose symptoms are of recent onset.


Assuntos
Plaquetas , Radioisótopos de Índio , Tromboflebite/diagnóstico por imagem , Reações Falso-Negativas , Reações Falso-Positivas , Feminino , Humanos , Masculino , Pessoa de Meia-Idade , Compostos Organometálicos , Oxiquinolina/análogos & derivados , Flebografia , Estudos Prospectivos , Cintilografia , Fatores de Tempo
17.
Cancer Genet Cytogenet ; 66(1): 43-6, 1993 Mar.
Artigo em Inglês | MEDLINE | ID: mdl-8467474

RESUMO

Chromosomal analysis was performed on the tumor tissue in a case of ocular rhabdomyosarcoma. Cytogenetic analysis revealed multiple clonal abnormalities. Derivative(5)t(1;5)(q21;q35) and t(2;11) (q21;q23) were present in all the metaphases analyzed (100%). Translocation(1;19)(q21;q13) was observed in 61% of the metaphases. Other clonal abnormalities present included +i(6p)(85%),i(17q)(38%),18q+(57%), 4p+ (38%), loss of the Y chromosome (33%), and presence of double minutes (12.8%).


Assuntos
Aberrações Cromossômicas , Neoplasias Oculares/genética , Rabdomiossarcoma/genética , Pré-Escolar , Cromossomos Humanos Par 1 , Humanos , Cariotipagem , Masculino , Translocação Genética
18.
Urology ; 15(6): 577-80, 1980 Jun.
Artigo em Inglês | MEDLINE | ID: mdl-7394984

RESUMO

A review of the literature reveals that patients with juvenile onset diabetes with azotemia, associated vascular disease, and proteinuria, are at great risk of sustaining acute renal failure following intravenous pyelography. Predisposing factors are identified, but the exact mechanism of acute renal failure remains obscure. Since no prophylactic measures or effective treatment exist, the benefits and risk must be weighed prior to the procedure.


Assuntos
Injúria Renal Aguda/etiologia , Diabetes Mellitus Tipo 1/complicações , Urografia/efeitos adversos , Meios de Contraste/efeitos adversos , Desidratação/complicações , Complicações do Diabetes , Angiopatias Diabéticas/complicações , Nefropatias Diabéticas/complicações , Humanos
19.
J Biotechnol ; 76(2-3): 137-46, 2000 Jan 21.
Artigo em Inglês | MEDLINE | ID: mdl-10656328

RESUMO

Freely suspended cells and immobilized cell cultures of Capsicum frutescens Mill. were treated with phenylpropanoid intermediates--protocatechuic aldehyde and caffeic acid to study their biotransformation ability. It was found that externally fed protocatechuic aldehyde and caffeic acids were biotransformed to vanillin and capsaicin. It was noted that this culture biotransformed externally fed protocatechuic aldehyde to vanillin more than its conversion to capsaicin, whereas, caffeic acid-treated cultures accumulated more capsaicin than vanillin. The maximum accumulation of vanillin (5.63 mg l(-1)) and capsaicin (3.83 mg l(-1)) was recorded on the 6th and 15th day, respectively in immobilized C. frutescens cell cultures treated with protocatechuic aldehyde, which was 1.8 and 1.4 times higher than in protocatechuic aldehyde-treated freely suspended cell cultures. Caffeic acid-treated immobilized C. frutescens cell cultures accumulated maximum vanillin and capsaicin at 2.68 and 3.03 mg l(-1) culture, respectively, on the 9th and 12th day, which was 1.65 and 1.33 times over freely suspended cultures treated with caffeic acid. The addition of S-adenosyl-L-methionine, a methyl donor, to protocatechuic aldehyde-treated immobilized C. frutescens cell cultures, resulted in accumulation of vanillin (14.08 mg l(-1)) on the 4th day, which was 2.5-fold higher than that in cultures treated with protocatechuic aldehyde alone, suggesting the influence of S-adenosyl-L-methionine on O-methylation of protocatechuic aldehyde, resulting in more vanillin accumulation. The increase in vanillin accumulation was well correlated with an increase in specific activity of caffeic acid O-methyltransferase in protocatechuic aldehyde and S-adenosyl-L-methionine-treated immobilized C. frutescens cell cultures. This study also provides an example for an alternative route to formation of vanillin by C. frutescens cell cultures.


Assuntos
Benzaldeídos/metabolismo , Benzaldeídos/farmacocinética , Ácidos Cafeicos/farmacocinética , Capsaicina/metabolismo , Capsicum/citologia , Capsicum/metabolismo , Catecóis/farmacocinética , Plantas Medicinais , Biotransformação , Capsicum/efeitos dos fármacos , Técnicas de Cultura de Células/métodos , Células Cultivadas , Metiltransferases/efeitos dos fármacos , Metiltransferases/metabolismo , S-Adenosilmetionina/farmacologia
20.
Mutat Res ; 282(2): 113-7, 1992 Jun.
Artigo em Inglês | MEDLINE | ID: mdl-1377349

RESUMO

The pattern of titrated thymidine (3H-TdR), a direct precursor of DNA, induced aberrations on the X chromosome of Gryllotalpa fossor was examined. 3H-TdR produced aberrations randomly distributed over the entire length of the X chromosome; breaks were observed in both the eu- and the heterochromatic arms of the X chromosome in both the sexes. Since the eu- and the heterochromatic arms cannot be distinguished cytologically in this insect, the presence of aberrations on both arms of the same X chromosome in the male and damage to both X chromosomes in the female indicate that both euchromatic and heterochromatic regions (facultative or constitutive) are equally liable to aberrations induced by H-TdR. This is in contrast to the non-random induction of aberrations by 3H-UdR, which causes chromosome damage due to the proximity of the labeled RNA to the DNA template during transcription.


Assuntos
Aberrações Cromossômicas , Mutagênese , Timidina/toxicidade , Trítio/toxicidade , Cromossomo X/efeitos da radiação , Animais , Feminino , Masculino , Ortópteros
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