Effects of replacing dietary fish meal with enzyme-treated soybean meal on growth performance, intestinal microbiota, immunity and mTOR pathway in abalone Haliotis discus hannai.

In addition to eliminating most of the anti-nutritional factors in soybean meal, enzyme-treated soybean meal (ESBM) can also increase the proportion of small peptides. It was found that ESBM can replace fish meal (FM) either partially or completely in diets for some fish and shrimp species. In the present study, the effects of replacing dietary FM with ESBM on growth performance, intestinal microbiota, immunity and mTOR pathway in abalone Haliotis discus hannai (initial weight: 16.75 ± 0.09 g) were investigated after a 100-day feeding trial. Five experimental diets were designed to replace 0%, 25%, 50%, 75% and 100% of dietary FM by ESBM, which were named as ESBM0 (control), ESBM25, ESBM50, ESBM75 and ESBM100, respectively. Results showed that ESBM could replace up to 75% of FM in the diet without significant effect on the weight gain rate (WGR, 118.05%-124.16%) of abalone. The increasing dietary ESBM levels significantly decreased the trypsin activity from 418.52 to 286.52 U/mg protein in the digestive gland. No significant differences in the contents of total cholesterol (T-CHO), ammonia (BLA) and malondialdehyde (MDA) in cell-free hemolymph were observed among the groups with replacement levels of dietary FM by ESBM from 0% to 75%. Excessive replacement level of FM with ESBM (ESBM100) significantly increased the MDA content (2.33 nmol/mg prot.) and pro-inflammatory-related gene expression in digestive gland. Compared with the control group, the mTOR pathway in muscle was significantly upregulated in the ESBM75 group. The digestive gland in the ESBM100 group contained more golden refractile spherules than those in the other groups. The abundance of intestinal microbes such as Halomonas, Zobellella and Bacillus was decreased in the ESBM100 group. In conclusion, up to 75% of replacement of dietary FM by ESBM had no negative effects on the growth performance, intestinal microbiota, immunity and mTOR pathway of abalone.

Ascorbic Acid Regulates the Immunity, Anti-Oxidation and Apoptosis in Abalone Haliotis discus hannai Ino.

The present study was conducted to investigate the roles of ascorbic acid (AA) in immune response, anti-oxidation and apoptosis in abalone (Haliotis discus hannai Ino). Seven semi-purified diets with graded levels of AA (0, 50, 100, 200, 500, 1000 and 5000 mg/kg) were fed to abalone (initial weight: 12.01 ± 0.001 g, initial shell length: 48.44 ± 0.069 mm) for 100 days. The survival, weight gain rate and daily increment in shell length were not affected by dietary AA. The AA content in the gill, muscle and digestive glands of abalone was significantly increased by dietary AA. In terms of immunity, dietary AA significantly improved the total hemocyte count, respiratory burst and phagocytic activity in hemolymph, and lysozyme activity in cell-free hemolymph (CFH). In the digestive gland, the TLR-MyD88-dependent and TLR-MyD88-independent signaling pathways were suppressed by dietary AA supplementation. The mRNA levels of ß-defensin and arginase-I in the digestive gland were significantly increased by dietary AA. In the gill, only the TLR-MyD88-dependent signaling pathway was depressed by dietary AA to reduce inflammation in abalone. The level of mytimacin 6 in the gill was significantly upregulated by dietary AA. After Vibrio parahaemolyticus infection, the TLR signaling pathway in the digestive gland was suppressed by dietary AA, which reduced inflammation in the abalone. In terms of anti-oxidation, superoxide dismutase, glutathione peroxidase and catalase activities, as well as total anti-oxidative capacity and reduced glutathione content in CFH, were all significantly upregulated. The malondialdehyde content was significantly downregulated by dietary AA. The anti-oxidative capacity was improved by triggering the Keap1-Nrf2 pathway in abalone. In terms of apoptosis, dietary AA could enhance the anti-apoptosis ability via the JNK-Bcl-2/Bax signaling cascade in abalone. To conclude, dietary AA was involved in regulating immunity, anti-oxidation and apoptosis in abalone.