Next-to-Next-to-Leading Order QCD Corrections to Semi-Inclusive Deep-Inelastic Scattering.

We present the first results for the next-to-next-to leading order (NNLO) corrections to the semi-inclusive deep-inelastic scattering process in perturbative quantum chromodynamics. We consider the quark initiated flavor nonsinglet process and obtain the complete contributions analytically at leading color. All relevant virtual and real emission Feynman diagrams have been computed using integration-by-parts reduction to master integrals and two approaches for their subsequent evaluation (parametric phase-space integration and method of differential equations). The numerical analysis demonstrates the significance of the NNLO corrections and their great impact on the reduction of the residual scale dependence.

Dietary fat lowers ileal endogenous amino acid losses in broiler chickens.

1. An experiment was conducted to determine the effect of the source of fat (soybean oil or tallow) on the ileal endogenous amino acid (EAA) losses in broilers.2. Three nitrogen (N)-free diets; a control diet with no added fat and test diets with 60 g/kg of either soybean oil or tallow were formulated. Titanium dioxide (5 g/kg) was added to all diets as an indigestible marker. Each diet was assigned to six replicate cages (eight birds per cage) from d 18 to 21 post-hatch. On d 21, the digesta were collected from the lower half of the ileum.3. The endogenous losses of nitrogen and amino acids (AA) were lower (p = 0.08; p = 0.001) in broilers fed diets with soybean oil or tallow, respectively, compared to those fed the diet with no fat. Source of fat had no influence (p > 0.05) on EAA losses.4. The most abundant AA in the ileal endogenous protein was glutamic acid, followed by aspartic acid, threonine, leucine, serine, valine and proline. In general, the concentrations of AA in the endogenous protein were lower (p < 0.05) with added fat. The exceptions were methionine, cysteine, proline and serine, which were unaffected. The effect of fat source on the AA contents of endogenous protein were inconsistent and differed depending on the AA.5. The inclusion of fats decreased EAA losses which implied they have beneficial effects beyond direct energy contribution. It can be proposed that the reduction of EAA flow may be an additional mechanism contributing to the extra-caloric effect of dietary fats.

The apparent metabolisable energy and ileal amino digestibility of black soldier fly (Hermetia illucens) larvae meal for broiler chickens.

1. Two experiments were conducted to determine the apparent metabolisable energy (AME) and standardised amino acid digestibility coefficients (SIDC) of black soldier fly larvae (BSFL) for broiler chickens.2. The BSFL contained, on a g/kg dry matter basis: crude protein, 486; crude fat, 320; ash, 58.5; neutral detergent fibre, 181; calcium, 6.8 and phosphorus, 9.1.3. In Experiment 1, an AME assay was performed wherein broilers were fed two experimental diets (a maize-soy basal diet and a test diet containing 250 g/kg BSFL) for 4 d from d 18 posthatch. The AME of BSFL was calculated based on the difference between the AME values of basal and test diets. The AME and nitrogen-corrected AME were determined to be 19.1and 18.0 MJ/kg of dry matter, respectively.4. In Experiment 2, the ileal amino acid (AA) digestibility of BSFL was determined using 22-d-old broilers by the direct method and the digesta was collected on d 25. The ratios between the AA and titanium in the diet and digesta were used to calculate the apparent digestibility and then standardised using previously published endogenous losses to estimate the SIDC of AA. The SIDC of Lys, Met, Thr, Val and average SIDC of AA in our BSFL sample were 0.85, 0.90, 0.91, 0.87 and 0.84, respectively.5. The findings showed that the BSFL meal is a good source of available energy and digestible AA, and could be a potential substitute for soybean meal in broiler diets.

Composition of chicken gallbladder bile.

1. The proximate composition and, amino acid, fatty acid and mineral profiles of gallbladder bile of 35 d old broiler chickens were determined.2. The dry matter (DM) content of the bile was determined to be 19.8 g/100 g. Fat, protein and ash contents in the bile were 24.9, 23.0 and 6.6 g/100 g DM, respectively.3. The content of total fatty acids (FA) in the bile was determined to be 17.68 g/100 g DM. Unsaturated FA dominated the profile in the bile, with an unsaturated: saturated FA ratio of 1.36. Oleic, linoleic, and arachidonic acids were the main unsaturated FA, whereas palmitic and stearic acids were the major saturated FA.4. A major finding was that the data established taurine as the primary amino acid in chicken bile and not glycine, as previously assumed. Taurine was the dominant amino acid, constituting 62% of bile protein.5. Among the major minerals, sodium (2.56 g/100 g DM) was in the greatest concentration. The concentrations of other major minerals were relatively low.6. The present work provided, for the first time, preliminary data on reference values for nutrients in the chicken gallbladder bile.

Comparison of the apparent ileal calcium digestibility of limestone in broilers and layers.

1. The apparent ileal calcium (Ca) digestibility coefficients of two limestone sources in growing broilers and layers were determined in two separate experiments.2. In each experiment, two maize-based diets were developed with two limestone sources (A, experiment 1 and B, experiment 2) to contain either 8.0 g/kg Ca for broilers or 40 g/kg Ca for layers. The two sources differed in particle size, with limestone A being finer and limestone B being coarser. Each experimental diet was randomly allotted to six replicate cages and offered for 3 days from 19 to 21-day post-hatch to broilers and during 40 weeks of age to layers. The total tract Ca retention was also measured using the indicator ratios in the diet and excreta.3. In both experiments, the apparent ileal Ca digestibility, gizzard pH and gizzard Ca concentration were higher (P < 0.05) in layers than in broilers. The apparent ileal digestibility coefficient of limestone A for broilers and layers was 0.50 and 0.62, respectively. The corresponding values for limestone B were 0.43 and 0.70, respectively. The apparent total tract retention of Ca was similar (P > 0.05) between broilers and layers in both experiments, and between the two sources.4. The data show that the layers are more efficient in absorbing Ca from limestone than broilers.

True ileal calcium digestibility in soybean meal and canola meal, and true ileal phosphorous digestibility in maize-soybean meal and maize-canola meal diets, without and with microbial phytase, for broiler growers and finishers.

1. Published data on the ileal Ca digestibility in soybean meal (SBM) and canola meal (CM), and the effect of microbial phytase on the Ca digestibility of these ingredients are limited. Therefore, two experiments were conducted, with the primary objective of determining the true ileal digestibility of calcium (Ca) in SBM and CM, without and with microbial phytase, during broiler grower (Experiment 1) and finisher (Experiment 2) periods. A secondary objective was to investigate the influence of microbial phytase on the true ileal digestibility of phosphorus (P), apparent digestibility of nitrogen (N) and minerals, and phytate disappearance in maize-SBM and maize-CM diets. Six experimental diets based on SBM and CM, with three phytase doses (0, 500 and 2000 FTU/kg), were fed to broilers from day 18 to 21 (Experiment 1) or 39 to 42 (Experiment 2) post-hatch. A Ca- and P-free diet, with no added phytase, was also developed to determine the endogenous Ca and P losses. Titanium dioxide was incorporated in all diets as an indigestible indicator. Each experimental diet was randomly allocated to six replicate cages (eight birds per cage). Apparent ileal digestibility was calculated using the indicator method and the true ileal digestibility was calculated by correcting for endogenous losses. Apparent total tract retention (ATTR) of Ca and P was also measured.2. Ileal endogenous losses of Ca and P were determined to be 236 and 310 mg/kg of dry matter intake (DMI), respectively, in broiler growers and 29 and 130 mg/kg of DMI, respectively, in broiler finishers. True ileal Ca digestibility coefficients of SBM and CM, without added phytase, were determined to be 0.51 and 0.53, respectively, in broiler growers and 0.33 and 0.22, respectively, in broiler finishers. Increasing phytase doses increased (P < 0.05) the true ileal Ca digestibility of CM in both broiler growers and finishers, but Ca digestibility of SBM increased (P < 0.05) only at the superdose (2000 FTU/kg) in broiler finishers. The ATTR of Ca (P < 0.001) in growers was higher in CM than in SBM and was increased in both ingredients by increasing phytase doses. In finishers, the ATTR of Ca was increased (P < 0.001) by both phytase doses in CM, but only by the superdose in SBM, resulting in an ingredient × phytase interaction (P < 0.001).3. True ileal P digestibility coefficients of maize-SBM and maize-CM diets, without added phytase, were determined to be 0.89 and 0.66, respectively, in broiler growers and 0.82 and 0.57, respectively, in broiler finishers. Supplemental phytase increased (P < 0.05) the true ileal P digestibility of the maize-CM diet in both broiler growers and finishers. However, the P digestibility of the maize-SBM diet was increased (P < 0.05) in broiler finishers only at the superdose (2000 FTU/kg). The ATTR of P was higher (P < 0.001) in the maize-SBM diet during both periods.4. The apparent ileal digestibility of N, Mg, K and Mn was higher (P < 0.001) in the maize-SBM diet for broiler growers and finishers. Phytase addition had no effect (P > 0.05) on the apparent digestibility of N and minerals in growers and finishers.5. Increasing phytase doses increased IP6 disappearance in the maize-CM diet, but not in the maize-SBM diet, resulting in an ingredient × phytase interaction (P < 0.001) for growers and finishers.6. In conclusion, true ileal Ca digestibility coefficients of SBM and CM for broilers were determined in this study. The findings confirmed the influence of broiler age of Ca digestibility. Superdosing of phytase increased the digestibility and ATTR of Ca in CM and SBM by two-fold compared to the normal phytase dose.

The effect of graded inclusions of waxy starch hull-less barley and a multi-component exogenous carbohydrase on the growth performance, nutrient digestibility and intestinal morphometry of broiler chickens.

1. A 21-d experiment was conducted to investigate the effect of graded inclusions of waxy starch hull-less (WSHL) barley and a multi-component exogenous carbohydrase on the growth performance, nutrient digestibility and intestinal morphometry of broiler chickens. Five levels of WSHL barley inclusion (0, 65, 130, 195 and 260 g/kg) in a wheat-based diet, and two levels of enzyme supplementation (0 and 150 g/tonne of feed) were evaluated in a 5 × 2 factorial arrangement of 10 dietary treatments. All diets were equivalent in metabolisable energy and digestible amino acid content. A total of 400, one-d-old male broilers (five cages/treatment; eight birds/cage) were used in the experiment. 2. Regardless of enzyme supplementation, feed intake declined (P < 0.001) with increasing inclusion of WSHL barley. Increasing levels of WSHL barley (P < 0.001) and supplemental enzyme (P < 0.01) increased gain to feed ratio. 3. Birds fed diets with 0 g/kg WSHL barley showed the lowest (P < 0.001 to 0.01) digestibility for all nutrients except starch. Only starch digestibility was improved (P < 0.05) by enzyme supplementation. The nitrogen-corrected apparent metabolisable energy improved with increasing inclusion of WSHL barley (P < 0.001) and supplemental enzyme (P < 0.001). Increasing inclusion of WSHL barley increased the relative weight of gizzard (P < 0.001) and reduced jejunal digesta viscosity (P < 0.01). Supplemental enzyme (P < 0.001) reduced digesta viscosity. 4. All levels of WSHL barley inclusion improved digestibility of dry matter, nitrogen and fat, whilst energy utilisation improved at inclusions of 130 g/kg WSHL and above, probably due to lowered digesta viscosity and better development of the gizzard. Gain to feed ratio, starch digestibility, energy utilisation and jejunal digesta viscosity can benefit from carbohydrase supplementation in wheat-based diets, regardless of barley inclusion level.

Nutritional evaluation of two barley cultivars, without and with carbohydrase supplementation, for broilers: metabolisable energy and standardised amino acid digestibility.

1. Two experiments were conducted to assess the nitrogen-corrected apparent metabolisable energy (AMEn; Exp. 1; 288 Ross 308 male broilers at d 14; 36 cages with eight birds each) and coefficient of standardised ileal digestibility (CSID) of amino acids (AA; Exp. 2; 336 Ross 308 male broilers at d 21; 42 cages with eight birds each) of two barley cultivars for broilers in comparison to wheat, without or with a multi-component non-starch polysaccharide (NSP) degrading enzyme. A 3 × 2 factorial arrangement of treatments was used in both experiments with three types of grains (normal starch hulled barley [NSH], waxy starch hull-less barley [WSHL], and wheat) and two levels of enzyme supplementation (0 and 200 g/tonne of feed). Enzyme supplemented diets contained 406 and 128 of endo-1, 4-ß-xylanase and endo-1, 3 (4)-ß-glucanase units per kg of feed, respectively. 2. Analysis showed that the starch content was higher in NSH (610 g/kg) than in wheat (537 g/kg) and WSHL (554 g/kg), and the composition of starch differed markedly among the grain types. The ß-glucan content was considerably higher in WSHL (68.6 g/kg) compared to NSH (38.5 g/kg) and wheat (7.74 g/kg). The contribution of soluble fraction to the total non-starch polysaccharides was higher in WSHL (38.2%) compared to NSH and wheat (17.1% and 13.3%, respectively). 3. A significant (P < 0.01) interaction was observed between the grain type and enzyme supplementation for AMEn. The WSHL, with the highest content of ß-glucan, showed the greatest response to enzyme supplementation for AMEn. 4. Birds fed wheat- and WSHL-based diets had the highest and lowest CSID of nitrogen and most of AA, respectively, with NSH diets being intermediate. Regardless of grain type, enzyme supplementation increased (P < 0.05) the CSID of nitrogen. 5. These data suggest that ß-glucan content plays an important role in determining the digestibility of nutrients in barley for broilers, resulting in a better feeding value for NSH over WSHL. Supplementation of a multi-component NSP-degrading enzyme can improve the feeding value of barley in broiler diets by increasing the digestibility with the effect being more pronounced in WSHL barley.

Influence of inclusion level of barley in wheat-based diets and supplementation of carbohydrase on growth performance, nutrient utilisation and gut morphometry in broiler starters.

1. The influence of barley inclusion level and supplementation of a multi-component non-starch polysaccharide degrading enzyme on performance and nutrient utilisation in broilers was investigated. Normal-starch hulled barley was evaluated with five levels of inclusion (0, 141, 283, 424 and 565 g/kg) in a wheat-based diet and two levels of enzyme supplementation (0 and 150 g/tonne of feed; a 5 × 2 factorial arrangement of 10 dietary treatments). All diets were equivalent in metabolisable energy and digestible amino acid contents. A total of 400, one-d old male broilers (five cages/treatment; eight birds/cage) were used in the experiment.2. Regardless of enzyme supplementation, weight gain (WG) increased up to 283 g/kg of barley and was reduced afterwards (P < 0.01). Increasing levels of barley resulted in greater (P < 0.001) gain per feed (G/F). Enzyme addition increased WG (P < 0.05) and G/F (P < 0.001) at each barley inclusion level.3. Birds fed diets with 0 and 565 g/kg barley showed the lowest and highest (P < 0.001to 0.05) digestibility for all nutrients measured, respectively. Digestibility of all nutrients was improved by enzyme supplementation at each barley inclusion level (P < 0.05). The nitrogen-corrected apparent metabolisable energy improved with increasing inclusion of barley (P < 0.001) and supplemental enzyme (P < 0.01). Increasing inclusion of barley increased the relative weight of gizzard (P < 0.001) and reduced jejunal digesta viscosity (P < 0.001). Supplemental enzyme (P < 0.001) reduced digesta viscosity.4. The optimum inclusion level of barley, with respect to growth performance, was 283 g/kg of diet. Increasing barley inclusion improved nutrient and energy utilisation, possibly through lowered digesta viscosity and better function of the gizzard. Feed efficiency and nutrient and energy utilisation can benefit from carbohydrase supplementation in barley-based diets, regardless of barley inclusion level.

Measurement of true ileal calcium digestibility in meat and bone meal for broiler chickens using the direct method.

The objective of the study that is presented herein was to determine the true ileal calcium (Ca) digestibility in meat and bone meal (MBM) for broiler chickens using the direct method. Four MBM samples (coded as MBM-1, MBM-2, MBM-3 and MBM-4) were obtained and analyzed for nutrient composition, particle size distribution and bone to soft tissue ratio. The Ca concentrations of MBM-1, MBM-2, MBM-3 and MBM-4 were determined to be 71, 118, 114 and 81 g/kg, respectively. The corresponding geometric mean particle diameters and bone to soft tissue ratios were 0.866, 0.622, 0.875 and 0.781 mm, and 1:1.49, 1:0.98, 1:0.92 and 1:1.35, respectively. Five experimental diets, including four diets with similar Ca concentration (8.3 g/kg) from each MBM and a Ca and phosphorus-free diet, were developed. Meat and bone meal served as the sole source of Ca in the MBM diets. Titanium dioxide (3 g/kg) was incorporated in all diets as an indigestible marker. Each experimental diet was randomly allotted to six replicate cages (eight birds per cage) and offered from d 28 to 31 post-hatch. Apparent ileal Ca digestibility was calculated by the indicator method and corrected for ileal endogenous Ca losses to determine the true ileal Ca digestibility. Ileal endogenous Ca losses were determined to be 88 mg/kg dry matter intake. True ileal Ca digestibility coefficients of MBM-1, MBM-2, MBM-3 and MBM-4 were determined to be 0.560, 0.446, 0.517 and 0.413, respectively. True Ca digestibility of MBM-1 was higher (P < 0.05) than MBM-2 and MBM-4 but similar (P > 0.05) to that of MBM-3. True Ca digestibility of MBM-2 was similar (P > 0.05) to MBM-3 and MBM-4, while that of MBM-3 was higher (P < 0.05) than MBM-4. These results demonstrated that the direct method can be used for the determination of true Ca digestibility in feed ingredients and that Ca in MBM is not highly available as often assumed. The variability in true Ca digestibility of MBM samples could not be attributed to Ca content, percentage bones or particle size.

Effect of limestone particle size and calcium to non-phytate phosphorus ratio on true ileal calcium digestibility of limestone for broiler chickens.

The purpose of this study was to determine the effect of limestone particle size and calcium (Ca) to non-phytate phosphorus (P) ratio on the true ileal Ca digestibility of limestone for broiler chickens. A limestone sample was passed through a set of sieves and separated into fine (<0.5 mm) and coarse (1-2 mm) particles. The analysed Ca concentration of both particle sizes was similar (420 g/kg). Six experimental diets were developed using each particle size with Ca:non-phytate P ratios of 1.5:1, 2.0:1 and 2.5:1, with ratios being adjusted by manipulating the dietary Ca concentrations. A Ca-free diet was also developed to determine the basal ileal endogenous Ca losses. Titanium dioxide (3 g/kg) was incorporated in all diets as an indigestible marker. Each experimental diet was randomly allotted to 6 replicate cages (8 birds per cage) and fed from d 21 to 24 post hatch. Apparent ileal digestibility of Ca was calculated using the indicator method and corrected for basal endogenous losses to determine the true Ca digestibility. The basal ileal endogenous Ca losses were determined to be 127 mg/kg of dry matter intake. Increasing Ca:non-phytate P ratios reduced the true Ca digestibility of limestone. The true Ca digestibility coefficients of limestone with Ca:non-phytate P ratios of 1.5, 2.0 and 2.5 were 0.65, 0.57 and 0.49, respectively. Particle size of limestone had a marked effect on the Ca digestibility, with the digestibility being higher in coarse particles (0.71 vs. 0.43).

Influence of partial replacement of ground wheat with whole wheat and exogenous enzyme supplementation on growth performance, nutrient digestibility and energy utilization in young broilers.

This study investigated the influence of pre-pelleting inclusion of whole wheat (WW) and exogenous enzyme supplementation on growth performance, coefficient of apparent ileal nutrient digestibility (CAID) and apparent metabolizable energy (AME) in broilers fed wheat-based pelleted diets. A 2 × 3 factorial arrangement of treatments was used with two methods of wheat inclusion [622 g/kg ground wheat (GW) and 250 g/kg WW replaced GW (wt/wt) pre-pelleting (PWW)] and three enzymes (xylanase, phytase and xylanase plus phytase). A total of 288, one-day-old male broilers (Ross 308) were individually weighed and allocated to 36 cages (8 broilers/cage), and the cages were randomly assigned to the six dietary treatments. Birds fed PWW diets gained more (p < 0.05) weight than those fed GW diets. There was no effect (p > 0.05) of WW inclusion on feed intake (FI). Phytase alone increased (p < 0.05) FI compared to xylanase or the combination. Whole wheat inclusion increased (p < 0.05) the gain-to-feed ratio (G:F). Feeding xylanase plus phytase and phytase-added diets resulted in the greatest and lowest G:F, respectively, with xylanase supplemented diets being intermediate. Birds fed PWW diets had greater (p < 0.05) relative gizzard weights than those fed GW diets. There was no effect (p > 0.05) of WW inclusion on the CAID of nitrogen (N), starch and fat. Combination of xylanase and phytase resulted in greater (p < 0.05) digestibility of N, starch and fat than that of individual additions. Feeding PWW diets resulted in greater (p < 0.05) AME values than GW diets. Combination of xylanase and phytase increased (p < 0.05) the AME compared to the diets with individual additions of xylanase or phytase. The current results suggest that the influence of pre-pelleting WW inclusion and exogenous enzymes on nutrient digestibility and broiler performance is not additive.

Influence of Palm Kernel Meal Inclusion and Exogenous Enzyme Supplementation on Growth Performance, Energy Utilization, and Nutrient Digestibility in Young Broilers.

The objective of the present study was to investigate the influence of palm kernel meal (PKM) inclusion and exogenous enzyme supplementation on growth performance, nitrogen-corrected apparent metabolizable energy (AMEn), coefficient of apparent ileal digestibility (CAID) and total tract retention of nutrients in young broilers fed corn-based diets. Four inclusion levels of PKM (no PKM [PKM0], 8% [PKM8], 16% [PKM16], and 24% [PKM24]) and two enzyme additions were evaluated in a 4×2 factorial arrangement of treatments. A total of 384, one-d-old male broilers (Ross 308) were individually weighed and allocated to 48 cages (eight broilers/cage), and cages were randomly assigned to eight dietary treatments. Results indicated that the inclusion of 8% and 16% PKM increased (p<0.05) the weight gain compared to the PKM0 diet. Birds fed the PKM8 diets had the highest (p<0.05) feed intake. Weight gain and feed intake were severely reduced (p<0.05) by feeding the PKM24 diet. Enzyme supplementation increased weight gain (p<0.05), independent of PKM inclusion level. In PKM0 and PKM8 diets, enzyme addition significantly (p<0.05) lowered feed conversion ratio (FCR); whereas enzyme addition had no effect on FCR of birds fed PKM16 and PKM24 diets. In PKM0 and PKM16 diets, enzyme addition significantly (p<0.05) increased CAID of nitrogen and energy but had no effect in the PKM8 and PKM24 diets. Inclusion of PKM into the basal diet, irrespective of inclusion level, enhanced (p<0.05) starch and fat digestibility. Inclusion of PKM at 16% and 24% resulted in similar CAID of neutral detergent fiber (NDF) but higher (p<0.05) than that of the PKM0 and PKM8 diets. Enzyme addition, regardless of the level of PKM inclusion, significantly (p<0.05) increased CAID of NDF. There was a significant (p<0.05) decrease in AMEn with PKM inclusion of 24%. The present data suggest that inclusion of PKM in broiler diets could be optimized if PKM-containing diets are formulated based on digestible amino acid contents and supplemented with exogenous enzymes. If amino acid digestibility and AME of PKM considered in the formulation, it can be included in broiler diets up to 16% with no deleterious effects on growth performance.

Effect of coccidia challenge and natural betaine supplementation on performance, nutrient utilization, and intestinal lesion scores of broiler chickens fed suboptimal level of dietary methionine.

The aim of the present experiment was to examine the effect of coccidia challenge and natural betaine supplementation on performance, nutrient utilization, and intestinal lesion scores of broiler chickens fed suboptimal level of dietary methionine. The experimental design was a 2×2 factorial arrangement of treatments evaluating two levels of betaine supplementation (0 and 960 g betaine/t of feed) without or with coccidia challenge. Each treatment was fed to 8 cages of 8 male broilers (Ross 308) for 1 to 21d. On d 14, birds in the 2 challenged groups received mixed inocula of Eimeria species from a recent field isolate, containing approximately 180,000 E. acervulina, 6,000 E. maxima, and 18,000 E. tenella oocysts. At 21d, digesta from the terminal ileum was collected for the determination of dry matter, energy, nitrogen, amino acids, starch, fat, and ash digestibilities. Lesion scores in the different segments of the small intestine were also measured on d 21. Performance and nutrient digestibility data were analyzed by two-way ANOVA. Lesion score data were analyzed using Pearson chi-square test to identify significant differences between treatments. Orthogonal polynomial contrasts were used to assess the significance of linear or quadratic models to describe the response in the dependent variable to total lesion scores. Coccidia challenge reduced (P<0.0001) the weight gain and feed intake, and increased (P<0.0001) the feed conversion ratio. Betaine supplementation had no effect (P>0.05) on the weight gain or feed intake, but lowered (P<0.05) the feed conversion ratio. No interaction (P>0.05) between coccidia challenge and betaine supplementation was observed for performance parameters. Betaine supplementation increased (P<0.05) the digestibility of dry matter, nitrogen, energy, fat, and amino acids only in birds challenged with coccidia as indicated by the significant interaction (P<0.0001) between betaine supplementation and coccidia challenge. The main effect of coccidia challenge reduced (P<0.05) starch digestibility. Betaine supplementation improved (P<0.05) starch digestibility regardless of the coccidia challenge. For each unit increase in the total lesion score, there was a linear (P<0.001) decrease in digestibility of mean amino acids, starch, and fat by 3.8, 3.4 and 16%, respectively. Increasing total lesion scores resulted in a quadratic (P<0.05) decrease in dry matter digestibility and ileal digestible energy. No lesions were found in the intestine or ceca of the unchallenged treatments. In the challenged treatments, betaine supplementation reduced (P<0.01) the lesion scores at the duodenum, lower jejunum, and total lesion scores compared to the treatment without supplements. In conclusion, coccidia challenge lowered the digestibility of energy and nutrients and increased the feed conversion ratio of broilers. However, betaine supplementation reduced the impact of coccidia challenge and positively affected nutrient digestibility and the feed conversion ratio.

Measurement of true ileal phosphorus digestibility in meat and bone meal for broiler chickens.

An experiment was conducted to estimate true ileal phosphorus (P:) digestibility of 3 meat and bone meal samples (MBM-1, MBM-2: , and MBM-3:) for broiler chickens. Four semipurified diets were formulated from each sample to contain graded concentrations of P. The experiment was conducted as a completely randomized design with 6 replicates (6 birds per replicate) per dietary treatment. A total of 432 Ross 308 broilers were assigned at 21 d of age to the 12 test diets. The apparent ileal digestibility coefficient of P was determined by the indicator method, and the linear regression method was used to determine the true P digestibility coefficient. The apparent ileal digestibility coefficient of P in birds fed diets containing MBM-1 and MBM-2 was unaffected by increasing dietary concentrations of P (P > 0.05). The apparent ileal digestibility coefficient of P in birds fed the MBM-3 diets decreased with increasing P concentrations (linear, P < 0.001; quadratic, P < 0. 01). In birds fed the MBM-1 and MBM-2 diets, ileal endogenous P losses were estimated to be 0.049 and 0.142 g/kg DM intake (DMI:), respectively. In birds fed the MBM-3 diets, endogenous P loss was estimated to be negative (-0.370 g/kg DMI). True ileal P digestibility of MBM-1, MBM-2, and MBM-3 was determined to be 0.693, 0.608, and 0.420, respectively. True ileal P digestibility coefficients determined for MBM-1 and MBM-2 were similar (P < 0.05), but were higher (P < 0.05) than that for MBM-3. Total P and true digestible P contents of MBM-1, MBM-2, and MBM-3 were determined to be 37.5 and 26.0; 60.2 and 36.6; and 59.8 and 25.1 g/kg, respectively, on an as-fed basis.

Influence of high dose of phytase and an emulsifier on performance, apparent metabolisable energy and nitrogen retention in broilers fed on diets containing soy oil or tallow.

The effects of high dose of microbial phytase and an emulsifier on the performance, apparent metabolisable energy (AME) and nitrogen (N) retention in broilers fed on diets containing different fat sources were examined in a 5-week trial. Two fat sources (soy oil and tallow), two inclusion levels of E. coli phytase (500 or 1000 phytase units (FTU)/kg diet) and two inclusion levels of lysolecithin emulsifier (0 or 3.5 g/kg of diet) were evaluated in a 2 × 2 × 2 factorial arrangement of treatments. Throughout the 5-week trial, soy oil supplementation improved weight gain and feed per gain compared with tallow, but had no effect on feed intake. The high dose of phytase increased the weight gain and feed intake and lowered the feed per gain during d 1-21, but had no effect on performance parameters over the whole trial period. An effect of emulsifier was observed for feed intake during d 1-21 and over the whole trial period. Addition of emulsifier increased feed intake compared with diets without emulsifier. During weeks 1, 2, 3 and 5, birds fed on soy oil-based diets had higher nitrogen-corrected AME (AMEN) compared with those fed on tallow-based diets. During weeks 2, 3 and 5, the effect of phytase was significant for AMEN, with the high dose increasing the AMEN. During week 2, AMEN was increased with emulsifier addition. During weeks 1, 2, 3 and 5, birds fed on soy oil-based diets had higher fat retention compared with those fed on tallow-based diets. The high dose of phytase improved the retention of fat during week 5 and the addition of emulsifier resulted in higher fat retention during week 1. During weeks 2, 3 and 5, an interaction between fat source × phytase × emulsifier was observed for N retention. In soy oil-based diets, emulsifier plus 1000 FTU/kg phytase increased N retention compared with other groups, while in tallow-based diets, emulsifier addition increased N retention in diets with 500 FTU/kg, but not in 1000 FTU/kg diet. Overall, the present data suggest that the dietary fat source influenced performance, AMEN and fat retention in broiler chickens. There is opportunity to improve bird performance during d 1-21, AMEN and fat retention with higher doses of microbial phytase. Addition of the emulsifier increased the AMEN during week 2 and tract retention of fat during week 1, but this effect was not translated into improvements in performance.

Drell-Yan production at threshold to third order in QCD.

The recent computation on the full threshold contributions to Higgs boson production at next to next to next to leading order (N^{3}LO) in QCD contains valuable information on the soft gluons resulting from virtual and real emission partonic subprocesses. We use those from the real emissions to obtain the corresponding soft gluon contributions to Drell-Yan production and determine the missing δ(1-z) part of the N^{3}LO. The numerical impact of threshold effects demonstrates the importance of our results in the precision study with the Drell-Yan process at the LHC.

Rapidity distributions in Drell-Yan and Higgs productions at threshold to third order in QCD.

We present the threshold N(3)LO perturbative QCD corrections to the rapidity distributions of dileptons in the Drell-Yan process and Higgs boson in gluon fusion. Sudakov resummation of QCD amplitudes, renormalization group invariance, and the mass factorization theorem provide useful guidelines to obtain them in an elegant manner. We use various state of the art three loop results that have been recently available to obtain these distributions. For the Higgs boson, we demonstrate numerically the importance of these corrections at the LHC.

Influence of tallow and calcium concentrations on the performance and energy and nutrient utilization in broiler starters.

The influence of tallow and Ca concentrations on the performance, apparent ileal digestibility, and total tract retention of N, Ca, P, and AME in broiler starter diets fed corn-soy-based diets was examined. The experimental design was a 3 × 3 factorial arrangement of treatments evaluating 3 inclusion levels of tallow (0, 40, and 80 g/kg) and 3 dietary concentrations of Ca (7, 10, and 13 g/kg). Nine treatment diets were formulated to meet the requirements for major nutrients for broiler starters, except for AME and Ca concentrations. The results showed that increasing tallow inclusion increased (P < 0.001) the weight gain and lowered (P < 0.001) the feed to gain. Increasing dietary Ca concentrations decreased (P < 0.001) the weight gain. Birds fed diets containing 7 g/kg of Ca had similar (P > 0.05) feed to gain to 10 g/kg of Ca but lower (P < 0.05) than that of 13 g/kg of Ca. In diets with no tallow, increasing Ca concentrations decreased (P < 0.05) feed intake, whereas diets with 40 and 80 g/kg of tallow containing 13 g/kg of Ca showed lower (P < 0.05) feed intake than those of 7 and 10 g/kg of Ca. Diets supplemented with 40 and 80 g/kg of tallow containing 7 g/kg of Ca showed the lowest (P < 0.05) excreta soap content. Total tract retention of fat was higher (P < 0.001) in diets with 40 g/kg of tallow compared with those with 0 and 80 g/kg of tallow. Birds fed diets containing 7 g/kg of Ca had similar (P > 0.05) fat retention to that of 10 g/kg of Ca, but higher (P < 0.05) than that of 13 g/kg of Ca. Calcium retention decreased (P < 0.001) with increasing Ca concentrations. Diets containing 13 g/kg of Ca had the lowest (P < 0.01) P retention. Diets with no inclusion of tallow containing 7 g/kg of Ca had higher (P < 0.05) N retention than that of 13 g/kg of Ca, but similar to 10 g/kg of Ca, whereas in 40 g/kg of tallow diets, 7 g/kg of Ca had the highest (P < 0.05) N retention. Increasing fat inclusion increased (P < 0.001) the soap content in ileal digesta. Diets with 40 g/kg of tallow had the highest ileal digestibility of fat (P < 0.001) and N (P < 0.01). Increasing inclusion of fat decreased (P < 0.001) ileal Ca digestibility. Diets containing 7 g/kg of Ca showed the highest ileal digestibility of fat (P < 0.01), N (P < 0.01), and P (P < 0.001). Birds fed diets containing 13 g/kg of Ca had higher ileal digestibility of Ca (P < 0.001) than those fed 7 and 10 g/kg of Ca diets. Overall, present data showed that the total tract retention and ileal digestibility of fat were higher with supplementation of 40 g/kg of tallow compared with those of 0 and 80 g/kg of tallow. High dietary Ca concentrations adversely affected the performance and the utilization of energy, N, Ca, and P in broiler starters.