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1.
J Exp Bot ; 64(8): 2423-34, 2013 May.
Artigo em Inglês | MEDLINE | ID: mdl-23606363

RESUMO

S-RNase-based gametophytic self-incompatibility evolved once before the split of the Asteridae and Rosidae. In Prunus (tribe Amygdaloideae of Rosaceae), the self-incompatibility S-pollen is a single F-box gene that presents the expected evolutionary signatures. In Malus and Pyrus (subtribe Pyrinae of Rosaceae), however, clusters of F-box genes (called SFBBs) have been described that are expressed in pollen only and are linked to the S-RNase gene. Although polymorphic, SFBB genes present levels of diversity lower than those of the S-RNase gene. They have been suggested as putative S-pollen genes, in a system of non-self recognition by multiple factors. Subsets of allelic products of the different SFBB genes interact with non-self S-RNases, marking them for degradation, and allowing compatible pollinations. This study performed a detailed characterization of SFBB genes in Sorbus aucuparia (Pyrinae) to address three predictions of the non-self recognition by multiple factors model. As predicted, the number of SFBB genes was large to account for the many S-RNase specificities. Secondly, like the S-RNase gene, the SFBB genes were old. Thirdly, amino acids under positive selection-those that could be involved in specificity determination-were identified when intra-haplotype SFBB genes were analysed using codon models. Overall, the findings reported here support the non-self recognition by multiple factors model.


Assuntos
Genes de Plantas/genética , Pólen/genética , Autoincompatibilidade em Angiospermas/genética , Sorbus/fisiologia , Sequência de Bases , Evolução Biológica , Genes de Plantas/fisiologia , Haplótipos/genética , Modelos Genéticos , Dados de Sequência Molecular , Filogenia , Pólen/fisiologia , Autoincompatibilidade em Angiospermas/fisiologia , Sorbus/genética
2.
PLoS One ; 8(5): e63747, 2013.
Artigo em Inglês | MEDLINE | ID: mdl-23696853

RESUMO

The Drosophila melanogaster G protein-coupled receptor gene, methuselah (mth), has been described as a novel gene that is less than 10 million years old. Nevertheless, it shows a highly specific expression pattern in embryos, larvae, and adults, and has been implicated in larval development, stress resistance, and in the setting of adult lifespan, among others. Although mth belongs to a gene subfamily with 16 members in D. melanogaster, there is no evidence for functional redundancy in this subfamily. Therefore, it is surprising that a novel gene influences so many traits. Here, we explore the alternative hypothesis that mth is an old gene. Under this hypothesis, in species distantly related to D. melanogaster, there should be a gene with features similar to those of mth. By performing detailed phylogenetic, synteny, protein structure, and gene expression analyses we show that the D. virilis GJ12490 gene is the orthologous of mth in species distantly related to D. melanogaster. We also show that, in D. americana (a species of the virilis group of Drosophila), a common amino acid polymorphism at the GJ12490 orthologous gene is significantly associated with developmental time, size, and lifespan differences. Our results imply that GJ12490 orthologous genes are candidates for developmental time and lifespan differences in Drosophila in general.


Assuntos
Proteínas de Drosophila/genética , Receptores Acoplados a Proteínas G/genética , Animais , Proteínas de Drosophila/classificação , Drosophila melanogaster , Evolução Molecular , Filogenia , Receptores Acoplados a Proteínas G/classificação
3.
J Integr Bioinform ; 9(3): 200, 2012 Jul 24.
Artigo em Inglês | MEDLINE | ID: mdl-22829571

RESUMO

Maximum-likelihood methods based on models of codon substitution have been widely used to infer positively selected amino acid sites that are responsible for adaptive changes. Nevertheless, in order to use such an approach, software applications are required to align protein and DNA sequences, infer a phylogenetic tree and run the maximum-likelihood models. Therefore, a significant effort is made in order to prepare input files for the different software applications and in the analysis of the output of every analysis. In this paper we present the ADOPS (Automatic Detection Of Positively Selected Sites) software. It was developed with the goal of providing an automatic and flexible tool for detecting positively selected sites given a set of unaligned nucleotide sequence data. An example of the usefulness of such a pipeline is given by showing, under different conditions, positively selected amino acid sites in a set of 54 Coffea putative S-RNase sequences. ADOPS software is freely available and can be downloaded from http://sing.ei.uvigo.es/ADOPS.


Assuntos
Aminoácidos/genética , Automação , Biologia Computacional/métodos , Seleção Genética , Software , Sequência de Aminoácidos , Sequência de Bases , Coffea/enzimologia , Coffea/genética , Dados de Sequência Molecular , Filogenia , Ribonucleases/química , Ribonucleases/genética , Alinhamento de Sequência , Interface Usuário-Computador
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