Utilizing a nurse practitioner to enhance diabetes management programs in home health.

There are challenges in caring for patients with diabetes. Home health should utilize nurse practitioners who have specialty diabetes programs for homebound individuals within their practice. Agencies benefit from designing specific disease management program models that provide standardized delivery of care with defined outcomes that can be evaluated for program effectiveness. Collaboration between programs like DOS and chronic disease management programs within home health agencies can achieve optimal patient outcomes and potential cost savings.

Microarray analysis for Saccharomyces cerevisiae.

In this protocol, gene expression in yeast (Saccharomyces cerevisiae) is changed after exposure to oxidative stress induced by the addition of hydrogen peroxide (H2O2), an oxidizing agent. In the experiment, yeast is grown for 48 hours in 1/2X YPD broth containing 3X glucose. The culture is split into a control and treated group. The experiment culture is treated with 0.5 mM H2O2 in Hanks Buffered Saline (HBSS) for 1 hour. The control culture is treated with HBSS only. Total RNA is extracted from both cultures and is converted to a biotin-labeled cRNA product through a multistep process. The final synthesis product is taken back to the UVM Microarray Core Facility and hybridized to the Affymetrix yeast GeneChips. The resulting gene expression data are uploaded into bioinformatics data analysis software.