Male moths bearing transplanted female antennae express characteristically female behaviour and central neural activity.

The primary olfactory centres of the sphinx moth Manduca sexta, the antennal lobes, contain a small number of sexually dimorphic glomeruli: the male-specific macroglomerular complex and the large female glomeruli. These glomeruli play important roles in sex-specific behaviours, such as the location of conspecific females and the selection of appropriate host plants for oviposition. The development of sexually dimorphic glomeruli depends strictly on the ingrowth of sex-specific olfactory receptor cell afferents. In the present study we tested the role of female-specific olfactory receptor cells (ORCs) in mediating female-specific host plant approach behaviour and in determining the response of downstream antennal lobe neurons. We generated male gynandromorphs by excising one imaginal disc from a male larva and replacing it with the antennal imaginal disc from a female donor. Most male gynandromorphs had an apparently normal female antenna and a feminised antennal lobe. These gynandromorphs were tested for flight responses in a wind tunnel towards tomato plants, a preferred host plant for oviposition in M. sexta. Male gynandromorphs landed on host plants as often as normal females, demonstrating that the presence of the induced female-specific glomeruli was necessary and sufficient to produce female-like, odour-oriented behaviour, i.e. orientation towards host plants. We also characterised the physiological and morphological properties of antennal lobe neurons of male gynandromorphs. We found that projection neurons with arborisations in the induced female-specific glomeruli showed physiological responses akin to those of female-specific projection neurons in normal females. These results therefore indicate that ORCs confer specific odour tuning to their glomerular targets and, furthermore, instruct odour-specific behaviour.

Triatoma infestans can be captured under natural climatic conditions using yeast-baited traps.

The effectiveness of a trap for triatomines baited with yeast cultures has been previously demonstrated in laboratory assays. We report results from assays testing yeast-traps for Triatoma infestans performed under natural climatic conditions. All assays were conducted at experimental chicken-coops colonised by bugs, situated at an endemic area for Chagas disease in Argentina. Two different models of traps were tested, for use either on the floor, or attached to walls. The results obtained clearly demonstrate that yeast-baited traps are effective for capturing triatomine bugs. Floor traps, when baited, captured significantly more bugs than their controls (t-test, P = 0.0008, k = 10). In addition, wall traps were also more effective, when compared to their controls (t-test, P = 0.011, k = 10). The maximum capture by a single yeast-trap was 39 bugs in one night, while for the same period the maximum capture by a control-trap was two bugs. Traps captured significantly more bugs at nights when maximum temperature was above 18 degrees C (t-test, P = 0.0002). Results indicate that yeast traps are capable of capturing significant numbers of free insects in one night. The potential use of yeast- traps for the detection of triatomine bugs is discussed.

Interaction of visual and olfactory cues in the aggregation behaviour of the haematophagous bug Triatoma infestans.

We analysed how the assembling behaviour of Triatoma infestans is modulated by the convergence of chemical cues released by their faeces and the spectral quality of the light associated with refuges. Second-instar larvae were confronted with refuges associated with a visual stimulus (either blue, green or red lights having the same intensity, or darkness) and a chemical cue (presence or absence of faeces). In this context, faeces constitute a major attractant for bugs. In the absence of faeces, bugs always assembled in dark places. Green light was always rejected despite the presence of faeces, i.e. the assembling behaviour was controlled by a photonegative reaction to this light. In the presence of red light, orientation towards the chemical cue dominated over the bugs' photonegative reaction to this light. Such a light was avoided in the absence of faeces but not in their presence. Also, negative phototaxis to blue light could be counteracted by the presence of the chemical cue. Thus, a concrete interaction between visual and olfactory cues occurred in these experiments: the bugs' response changed depending on the specific combination of spectral light and faeces. Finally, bugs responded differentially to blue, red and green lights of the same intensity. They assembled preferentially on red, followed by blue and then by green. Thus, discrimination between lights of different spectral quality is possible, probably through an achromatic mechanism.