Studying Force Patterns in an Alpine Ski Boot and Their Relation to Riding Styles and Falling Mechanisms.

In skiing, performance and safety can depend on small details. Consequently, the measurement of forces within the ski boots, which represent the essential form-fitting and force transmitting interface during skiing, will lead to enhanced performance and more importantly safety. This study presents a methodology to measure force patterns (continuous data acquisition) under laboratory as well as realistic slope conditions. The force measurements will be analyzed to gain insights of the skiing style, skiing technique, specific falling mechanisms (i.e., boot induced anterior drawer, phantom foot, hyperextension of the knee joint, and valgus-external rotation). Furthermore, the locations of force sensors in a overlap designed ski boot are discussed in terms of practicability and applicability. These insights are of particular interest to derive release conditions for predictive binding systems and furthermore provide data to improve the style of skiing (e.g., turn release action or center of gravity behavior). For that purpose, a ski boot was instrumented with seven force (piezoresistive) sensors while the basic structure of the boot and the binding remained unchanged. Three sensors were placed on the insole to measure ground reaction forces as well as the contact forces between the skier's foot and the boot. The other four sensors were positioned at spoiler/shaft and toecap (front sole) regions of the ski boot. The locations of the force sensors within the ski-boot are defined with regard to the main body movement while skiing (body-related planes). In addition, a commercially available ski and body mount measuring system were utilized to correlate speed, inclination and body position with the force patterns occurring during skiing on the slope as well as simulating specific body positions on an inclined ramp under laboratory conditions. The measured force revealed that the toecap (upper) sensors provide insufficient even non-conclusive data to deduce significant patterns. However, the insole sensors (heel and front sole area) as well as the spoiler/shaft (back) sensors are more reliable and show characteristic patterns indicating forward or backward lean. These results will have an important impact to the development of predictiveelectro-mechanical bindings to prevent knee-related injuries, which, from a statistical point of view, concerns largely women and young athletes.

Don't trust an(t)ybody - Pitfalls during investigation of candidate proteins for methylmercury transport at the placental interface.

While investigating placental mercury transport, we validated specificity of commercial antibodies against four candidate transporters (Large neutral amino acids transporter (LAT)1, LAT2, 4F2 cell-surface antigen heavy chain (4F2hc), and multidrug resistance-associated protein (MRP)2) by immunoblotting and small interfering RNA (siRNA)-mediated protein knockdown. An anti-4F2hc- and one anti-LAT1-antibody were specific. Another anti-LAT1-antibody reacted with LAT2. Two anti-LAT2-antibodies detected mainly albumin in placental lysates. A specific anti-MRP2-antibody hardly detected MRP2 in human placentas, contradicting published data. We recommend testing any unknown antibody by western blotting for 1/specificity for the protein of interest using e.g. siRNA knockdown and 2/cross-reactivity with albumin.