[Effects of high-sucrose/high-fat diet feeding time on establishment of a mouse model of gestational diabetes mellitus].

OBJECTIVE: The present study focuses on the effects of different highsucrose/high-fat diet feeding time on body weight, glucose and lipid metabolism parameters and pathological changes of pancreas and liver in pregnant mice. METHODS: Female C57 BL/6 J mice were divided into three groups randomly: normal control group( C) and two different high-sucrose/high-fat diet feeding time groups( H1 and H2). Body weight, food and energy intakes and blood glucose were measured at multiple time points before and during pregnancy. At the 17 thday of pregnancy, the mice were killed after blood collection. The parameters about glucose and lipid metabolism and serum insulin level were studied. Meanwhile, pancreas and liver tissues were collected for pathological examination. RESULTS: The body weight of group H1 and group H2 were respectivelyhigher than that of group C at multiple time points before pregnancy. With the increase of high-sucrose/high-fat diet feeding time, the tendency of body weight during pregnancy was showed as: group H2 > group H1 > group C. At the 10 thday of pregnancy, the tendency of blood glucose level was showed as: group H2 > group H1 > group C. At the 17 thday of pregnancy, there was no significant difference in blood glucose and insulin levels among the three groups. However, with the increase of high-sucrose/high-fat diet feeding time, the score of ß-cell function had a decreasing trend. Furthermore, the LDL-C level in group H1 and group H2 was significantly higher than that in group C respectively. Histopathological observations showed that the morphological structures of pancreas and liver in group C were normal. However, hypertrophy of pancreatic islets and fatty deposition of liver were existed in the other two groups, and the pathological changes in group H2 were more serious than those in group H1. CONCLUSION: By controlling the feeding time of high-sucrose/high-fat diet, the progressive development of gestational diabetes mellitus in C57 BL/6 J mice could be observed roughly.

[Validation of an online dietary assessment tool].

OBJECTIVE: To assess the validity of eat-right assistant( ERA) as an online dietary assessment tool. METHODS: Women employees in child-bearing age in a company in Shanghai and pregnant women who received regular antenatal care, lactating women whose children had physical examination in a village hospital in Suzhou were recruited into this study. They recorded their food and drink intakes on the ERA and completed 24-hour dietary recalls for three days consecutively. The energy, nutrient and food group intakes of the two methods were calculated for further comparisons. RESULTS: A total of ninety participants completed the study. Except for fruit intake, no significant differences were found between the two methods for mean intakes of energy and nutrients as well as other food intakes. Correlation coefficients ranged from 0. 61- 0. 93( mean0. 75). Bland-Altman plots showed agreement between the two methods. CONCLUSION: ERA is a relatively reliable tool for dietary assessment and is potential to be applied in target population.