Histomonas meleagridis in turkeys: dissemination kinetics in host tissues after cloacal infection.

Histomonas meleagridis is a flagellated protozoa causing histomoniasis, a disease of gallinaceous fowl. This disease is characterized by necrotic typhlitis, hepatitis, and high mortality, especially in turkeys. In an attempt to detect the progression of H. meleagridis in the turkey, birds were infected via the cloaca. Between d 0 and 19, a group of 4 turkeys was killed and autopsied every 3 d. Cecal and hepatic lesion scores were used to measure severity of infection. For each turkey, 15 tissue samples were taken. Another group of 3 infected turkeys were placed separately, and samples of cecal and intestinal stool were collected every 3 d. Samples were analyzed by PCR. For samples of cecal droppings, cecum, cecal content, rectum, proventriculus, and bursa of Fabricius, the number of birds detected as positive by PCR followed the evolution of the lesion scores. Within the liver, the parasite DNA was detected only in some severe lesions. The parasite DNA was also detected in duodenum, jejuno-ileum, spleen, heart, lungs, and brain samples. The parasite DNA was not detected in the blood, kidneys, pancreas, or muscle of the thigh. Results of the PCR were in agreement with the evolution of the clinical signs and of the cecal and liver lesions.

Fungal isolation and identification in 21 cases of guttural pouch mycosis in horses (1998-2002).

This aetiological study of guttural pouch mycosis (GPM) in the horse was based on the retrospective study of 21 horses brought into the National Veterinary School of Lyon (France) between 1998 and 2002. Biopsies were taken from the lesions caused by GPM during endoscopic examination. In 87% of the cases, direct examination gave positive results, whereas 43% of the cultures were found to be negative. The main fungi observed were Aspergillus fumigatus (in three cases), A. versicolor (in two cases, together with other fungi), and A. nidulans and A. niger (one case each). In six cases, the Aspergillus species could not be identified. In two cases, cleistothecia and/or Hulle cells were observed. In three cases, fungi other than Aspergillus were seen, mixed or not with Aspergillus. These results underline the importance of Aspergillus fumigatus in the development of GPM in horses.

Efficacy of selamectin administered topically in the treatment of feline otoacariosis.

The efficacy of a novel avermectin, selamectin (Stronghold, Pfizer), was evaluated against naturally acquired aural infestations of Otodectes cynotis. Selamectin was administered topically in a single spot to the skin of each animal's back at the base of the neck in front of the scapulae at a minimum dosage of 6mgkg(-1). Thirty cats of a cattery of 120 animals with an endemic infestation were treated on days 0 and 30. Including untreated control cats was therefore not possible because of animal welfare consideration. Clinical examination and visualization of mites by otoscopic examination of the external ear canal and microscopic examination of aural debris/exudate were performed twice weekly from days 0 to 30. On day 0 (and day 28 if the ears canals were erythematous), a swab was taken from each canal and sent to bacteriology and mycology units for detection and identification of bacteria (Staphylococcus mainly) and yeast (Malassezia pachydermatis only). Selamectin was safe, parasites were killed before day 3 and eliminated before day 17. Selamectin was 100% effective against natural aural infestations, even if erythema (26-33% of cats) and scratch reflex (23-40% of cats) persisted for 2 weeks after the cats tested negative for mites.

Administration of toltrazuril during experimental coccidiosis in mule ducks: comparison of the efficacy of a single administration at two different endogenous stages.

An experimental Eimeria mulardi coccidiosis was reproduced in mule ducks. A single dose of toltrazuril (7 mg per kg) was administered at two different endogenous stages: Day 2 (first meronts) or Day 5 (last meronts and gamonts). The efficacy of the drug was assessed on four criteria (body weight, oocyst counts, macroscopic lesions, and presence of the meronts and gamonts). To have a curative effect, a single dose of toltrazuril should be administered early on. However, a single treatment on Day 5 had a prophylactic effect.

[Description of Eimeria mulardi n. sp. from the hybrid duck. Study of the endogenous phase of its developmental cycle with demonstration of intranuclear development]. / Description d'Eimeria mulardi n. sp. chez le canard mulard. Etude de la phase endogène de son cycle évolutif avec mise en évidence du développement intranucléaire.

A new species of coccidium. Eimeria mulardi n. sp., has been described from the mule duck. The endogenous cycle is studied in experimentally infected ducks. A least two generations of meronts were observed, mainly in the lamina propria of jejunum and ileum and the gametogony occurred principally in glandular cells of the intestinal tract, except in duodenum. All the endogenous stages were localized in the nucleus of the cell.

Infectivity of Histomonas meleagridis in ducks.

The susceptibility of mule and muscovy ducks to "blackhead" disease caused by Histomonas meleagridis was studied, using an experimental intracloacal inoculation. Turkeys were used as controls. Morbidity, mortality and body weight gain were recorded regularly during the experiments. A direct examination of the caecal content was made to determine the absence or presence of the parasite. Gross and microscopic lesions were observed on days 7, 14, 21, 28 and 35 post infection to evaluate any clinical histomoniosis in ducks and to appraise the histomonad's carriage. A scoring system was developed both for gross and histological lesions of the caecum and liver. Infected mule and muscovy ducks (n = 83) never developed any clinical signs of histomoniasis. Weight gains of infected mule and muscovy ducks were similar to those of uninfected ducks. In 67% of the ducks (56/83), it was possible to demonstrate the parasite in the caecal content throughout the experiment. Typical macroscopic caecal lesions were observed in five of the ducks between days 7 and 21 post infection, with a caecal necropsy main lesion score (MLS = 1.6) less severe than that in turkeys (MLS = 2.9). Only caecal histological lesions occurred in six of the cases. Therefore, ducks do not seem to be a susceptible host for "blackhead" but may act as carrier animals for H. meleagridis. The virulence was apparently not changed, since 67% of turkeys (10/15) infected with the caecal content of positive ducks displayed classical signs of blackhead disease. Even if H. meleagridis alone does not represent a substantial danger in the duck production, its infectivity should to be taken into account in the transmission to more susceptible species.

[Coccidia of mule ducks: preliminary survey in three farms of the south-west region of France]. / Les coccidies du canard mulard. Bilan d'une premiere enquete realisee dans trois elevages du sud-ouest de la France.

Coccidia of mule ducks: preliminary survey in three farms of the south-west region of France A coprological survey carried out on mule duck farms, has revealed seven different coccidian species: Cryptosporidium (1), Tyzzeria (2) and Eimeria (4). Tyzzeria was the most frequent. The four Eimeria species have never been previously noted in France and one of them could be a new species.

Electron microscopic study on the endogenous development of Eimeria mulardi, Chauve, Reynaud and Gounel, 1994: a coccidium from the mule duck.

An electron microscopic study of the endogenous development of Eimeria mulardi Chauve, Reynaud and Gounel, 1994 was carried out in mule ducks which are hybrids of the domestic duck (Anas platyrhynchos) and the muscovy duck (Cairina moschata). All of the endogenous stages were seen within the nucleus of the host cell. Merozoites arose from ectomerogony and three mutually similar merogonies were noted. The asexual stages were found in leukocyte-like cells in the lamina propria of the jejunum, ileum and caecum, while the gamonts developed in glandular epithelial cells in the same part of the intestine.

[Pathogenicity of Eimeria mulardi Chauve et al., 1994 in experimentally infected mule-ducks. Precision on pathological stages]. / Pathogenicite d'Eimeria mulardi Chauve et al., 1994 chez le canard mulard apres infection experimentale. Precision des stades pathogenes.

Pathogenicity of Eimeria mulardi Chauve et al., 1994 in experimentally infected mule-ducks. Precision on pathological stages The pathogenicity of Eimeria mulardi has been studied in mule-ducklings orally inoculated with sporulated oocysts. With two dose levels (10(5) or 10(6) oocysts per bird), a significant loss of body-weight was observed, whereas no loss of weight was observed with a dose level of 10(4). With a dose of 10(6) oocysts per bird, inflammatory and haemorrhagic changes associated with obstruction mainly involving the end of the jejunum, the ileum and the caeca were seen from day 5 post-inoculation. Pathogenicity of Eimeria mulardi is due to the last generation of meronts and essentially to gamonts.

[Isolation of 2 coccidia of the genus Isospora from ducks in France]. / Isolement de 2 coccidies du genre Isospora chez des canards en France.

The authors isolated 2 Isospora species from ducks: Isospora mandari and Isospora sp. This was the first report of the genus Isospora from the duck in France. One of the 2 isolated species (Isospora sp) has not been described in the duck before.

Isospora sp from ducks. Infectivity for the goose, four anatids and the domestic fowl.

A coccidium was isolated from mule duck farms, the sporulated oocysts of which most closely resemble that of Isospora anseris, a goose coccidium. By experimental infection, these oocysts were proved to be able to develop in the goose and in several duck species, but unable to develop in the chicken. No pathogenicity was noted in any species.