Linking chemical exposure to lipid homeostasis: A municipal waste water treatment plant influent is obesogenic for zebrafish larvae.

Obesity, a risk factor for the development of type-2 diabetes, hypertension, cardiovascular disease, hepatic steatosis and some cancers, has been ranked in the top 10 health risk in the world by the World Health Organization. Despite the growing body of literature evidencing an association between the obesity epidemic and specific chemical exposure across a wide range of animal taxa, very few studies assessed the effects of chemical mixtures and environmental samples on lipid homeostasis. Additionally, the mode of action of several chemicals reported to alter lipid homeostasis is still poorly understood. Aiming to fill some of these gaps, we combined an in vivo assay with the model species zebrafish (Danio rerio) to screen lipid accumulation and evaluate expression changes of key genes involved in lipid homeostasis, alongside with an in vitro transactivation assay using human and zebrafish nuclear receptors, retinoid X receptor α and peroxisome proliferator-activated receptor γ. Zebrafish larvae were exposed from 4 th day post-fertilization until the end of the experiment (day 18), to six different treatments: experimental control, solvent control, tributyltin at 100 ng/L Sn and 200 ng/L Sn (positive control), and wastewater treatment plant influent at 1.25% and 2.5%. Exposure to tributyltin and to 2.5% influent led to a significant accumulation of lipids, with white adipose tissue deposits concentrating in the perivisceral area. The highest in vitro tested influent concentration (10%) was able to significantly transactivate the human heterodimer PPARγ/RXRα, thus suggesting the presence in the influent of HsPPARγ/RXRα agonists. Our results demonstrate, for the first time, the ability of complex environmental samples from a municipal waste water treatment plant influent to induce lipid accumulation in zebrafish larvae.

Calvarial autogenous bone graft for maxillary ridge and sinus reconstruction for rehabilitation with dental implants.

Autogenous bone grafting is the gold-standard technique for bone augmentation procedures prior to implant placement. If the amount of available intraoral donor bone is insufficient, it is necessary to harvest bone graft from extraoral sites, such as calvaria. Although this technique is well established, only a few case reports show the histological analysis of the grafted bone at the moment of implant placement. This article reports the case of a 48-year-old female patient with a critical atrophic maxillary ridge reconstructed using autogenous calvarial bone graft prior to implant placement, with clinical and histological evaluation. Bone was collected under general anesthesia from the parietal bone. The outer cortical originated the bone blocks, and the medullar bone layer between was collected to be used in the sinus augmentation procedure, together with 5 of the bone blocks triturated. Six months after bone augmentation, 8 implants were placed in the grafted area and 2 biopsies were retrieved (anterior and the posterior regions), allowing the visualization of the bone-remodeling process in the grafted areas. The patient had a stable recovery. Our results showed that although necrotic bone could still be seen in the outer layer of the grafted area, the interface between this necrotic bone and the already remodeled bone was consistent with biocompatibility. Two-year radiographic evaluation showed success of the grafts and the implants in supporting an esthetic and functionally stable prosthesis. Summarizing, calvarial bone grafts are a viable alternative for the attainment of adequate bone volume prior to implant placement.

Membrane Physiology Symposium April 22nd-23rd, 2024, Napa California, USA.

The Membrane Physiology Symposium was created with the goal of joining basic research with technology companies, where questions and conversations are open and welcomed in a universal language. For many years, academic physiology research areas have been naturally siloed into their own niche communities, which can surely be beneficial. Linking different technological application areas with varied research sectors is an integral formula for successful scientific breakthroughs. The meeting covers a wide variety of topics related to channelopathies, neurological and cardiac disease, drug development, and therapeutic applications, with research programs represented by core academic facilities, medical science institutions, small and large pharmaceutical enterprises, as well as novel cell-based and reagent providers. For this reason, gathering the brightest minds of all relevant fields in one integrative forum is essential for new avenues of discovery, development, and process optimization to occur.

Elucidating the Mechanisms of Pulsed Radiofrequency for Pain Treatment.

Pulsed radiofrequency is a well-documented treatment option for multiple painful conditions where pulses of energy are delivered close to neural elements. Since its earliest adoption, this technique has gained increasing acceptance as a minimally invasive procedure, and new applications are evolving. Studies have shown microscopic and biochemical changes that reflect beneficial effects; however, the exact mechanism of action is not yet completely understood. To redress this paucity, 11,476 articles of scientific relevance published between 1980 and November 2022 were mined through a search of the PubMed database, arriving at 49 studies both in animals and humans. In general, the experimental studies examined have shown that pulsed radiofrequency induces multiple changes with antinociceptive and neuromodulatory effects. These modifications include changes in neural and glial cells, synaptic transmission, and perineural space. Studies also reveal that pulsed radiofrequency regulates inflammatory responses, cellular signaling proteins, and the expression of genes related to pain transmission, acting in biological processes in structures such as myelin, mitochondria, axons, glial cells, connective tissue, regulation of proteins, ion channels, and neurotransmitters.

Human non-decalcified histology of three dental implants 45 months under function-a case report.

BACKGROUND: Fracture of an implant is a quite rare event but represents an important opportunity to evaluate the peri-implant bone tissue response to implant overload in human beings. This study aimed to evaluate bone tissue around three fractured titanium implants retrieved from a human maxilla, by histomorphometric and birefringence analyses. CASE REPORT: For this, the implants and the surrounding bone were removed after having been united to a tooth in function for 45 months, by a 4-mm internal diameter trephine bur, following an undecalcified section was obtained. The results showed a rate of 77.3% of bone-to-implant contact (BIC) and 80.3% of bone area filling within the limits of the implant threads. Under circularly polarized light microscopy investigation, the amount of the transverse collagen fibers was of 48.11%, and the amount of the longitudinal collagen fibers was of 51.89%. CONCLUSION: Within the limitation of this study, the possible cause of the implant fracture could be the association of overload, inadequate implant diameter, and fragile internal hexagon connection.

Histomorphometric evaluation of human sinus floor augmentation healing responses to placement of calcium phosphate or Ricinus communis polymer associated with autogenous bone.

BACKGROUND: Prosthetic rehabilitation of the posterior maxilla with dental implants is often difficult because of proximity to the maxillary sinus and insufficient bone height. Maxillary sinus floor augmentation procedures aim to obtain enough bone with an association between biomaterials and autogenous bone. PURPOSE: The purpose of this study was to evaluate histomorphometrically two grafting materials (calcium phosphate and Ricinus communis polymer) used in maxillary sinus floor augmentation associated with autogenous bone. MATERIALS AND METHODS: Biopsies were taken from 10 consecutive subjects (mean age 45 years) 10 months after maxillary sinus floor augmentation. The sinus lift was performed with a mixture of autogenous bone and R. communis polymer or calcium phosphate in a 1:2 proportion. Routine histologic processing and staining with hematoxylin and eosin were performed. RESULTS: The histomorphometric analysis indicated satisfactory regenerative results in both groups for a mean of bone tissue in the grafted area (44.24 +/- 13.79% for the calcium phosphate group and 38.77 +/- 12.85% for the polymer group). Histologic evaluation revealed the presence of an inflammatory infiltrate of mononuclear prevalence that, on average, was nonsignificant. The histologic sections depicted mature bone with compact and cancellous areas in both groups. CONCLUSION: The results indicated that both graft materials associated with the autogenous bone were biocompatible, although both were still present after 10 months.

Histomorphometrical analysis of bone formed after maxillary sinus floor augmentation by grafting with a combination of autogenous bone and demineralized freeze-dried bone allograft or hydroxyapatite.

BACKGROUND: Maxillary sinus floor augmentation procedures are currently the treatment of choice when the alveolar crest of the posterior maxilla is insufficient for dental implant anchorage. This procedure aims to obtain enough bone with biomaterial association with the autogenous bone graft to create volume and allow osteoconduction. The objective of this study was to histologically and histometrically evaluate the bone formed after maxillary sinus floor augmentation by grafting with a combination of autogenous bone, from the symphyseal area mixed with DFDBA or hydroxyapatite. METHODS: Ten biopsies were taken from 10 patients 10 months after sinus floor augmentation using a combination of 50% autogenous bone plus 50% demineralized freeze-dried bone allograft (DFDBA group) or 50% autogenous bone plus 50% hydroxyapatite (HA group). Routine histological processing and staining with hematoxylin and eosin and Masson's trichrome were performed. RESULTS: The histomorphometrical analysis indicated good regenerative results in both groups for the bone tissue mean in the grafted area (50.46+/-16.29% for the DFDBA group and 46.79+/-8.56% for the HA group). Histological evaluation revealed the presence of mature bone with compact and cancellous areas in both groups. The inflammatory infiltrate was on average nonsignificant and of mononuclear prevalence. Some biopsies showed blocks of the biomaterial in the medullary spaces close to the bone wall, with absence of osteogenic activity. CONCLUSIONS: The results indicated that both DFDBA and HA associated with an autogenous bone graft were biocompatible and promoted osteoconduction, acting as a matrix for bone formation. However, both materials were still present after 10 months.

Biological markers for evaluating therapeutic efficacy in Chagas disease, a systematic review.

The most neglected aspects of Chagas disease (CD) have been patient care and treatment. Despite recent progress in the development of potentially improved drugs, there is no consensus among different research groups on the lack of therapeutic response markers to evaluate efficacy of newly proposed drugs early after treatment. A systematic review of current evidence regarding molecules which are potential biomarkers for therapeutic response has been conducted using quality assessment and target responses as primary criteria. The review provides a panorama of the cumulative evidence and specific needs for development of a battery of complementary biomarkers which together fulfill ideal or acceptable criteria to evaluate early responses to treatment for chronic CD. There are several marker candidates which together may fulfill acceptable criteria to indicate the efficacy of a trypanocidal treatment. Data from ongoing studies are considered essential to improve assessment of existing markers and to identify those for early follow-up of treated patients.

In vitro evaluation of the antimicrobial activity of Basil ( Ocimum baslicum L. ) and Coriander ( Coriandrum satirum L. ) oil extracts on Streptococus mutans

Plant extracts and essential oils show efficiency on growth control in a wide variation of microorganisms, including filamentous fungi, yeasts and bacteria. To evaluate antimicrobial activity of plant extracts, determine the lower quantity of substance to inhibit the microorganism test growth is necessary. This value is known as Minimum Inhibitory Concentration (MIC). This study had as aim to verify the antimicrobial action and the Minimum Inhibitory Concentration (MIC) of basil (Ocimum basilicum L.) and coriander (Coriandrum sativum L.) oil extract before S. mutans (ATCC 25175) strains. Antimicrobial activity determination was carried out by microdilution method and performed according to recommendations of CLSI (previously known as NCCLS), standard M7-A6 (NCCLS, 2003) for bacteria, and standard M27-A2 (NCCLS, 2002). All the experiments were carried out in triplicate. Results showed the Minimum Inhibitory Concentration (MIC) determination by microdilution method in broth showed Ocimum basilicum L. and Coriandrum sativum L. extract oils presented inhibitory activity before S. mutans strain. Basil in 1:4 concentration is bacteriostatic and in 1:3 concentration is bactericide. Coriander in 1:2 concentration is bacteriostatic and in 1:1 concentration is bactericide. We concluded that basil presented higher inhibitory activity regarding to the coriander. We also observed as bigger the extract dilution, lower their effectivity.o assess the attitude and practice of dental professionals towards using of advance Radiographic technique.

In situ evaluation of basil oil essential oil cytotoxicity ( Ocimum basilicum L. )

Essential oils derived from plants used as condiments represent a large group of natural antimicrobials. Extracts and essential oils from plants have been shown to be efficient in controlling the growth of a wide variety of microorganisms, including filamentous fungi, yeasts and bacteria. Practical uses of these activities are suggested in humans and animals, as well as in the food industry. This study aimed to evaluate the basal basil essential oil (Ocimum basilicum L.) cytotoxicity in NCTC Clone 929 cell line, mouse connective tissue cells (CCIAL 020), which were seeded in Petri dishes and incubated for 48 hours To to form the cell monolayer. For the this test accomplishment of this test, we counted with the collaboration of the Nucleus of Cell Cultures - NCC of the Adolfo Lutz Institute, of the city of São Paulo-SP. The culture medium used is the solid coating medium, and fragments of the samples fragments are were placed on this coating medium and the plates were again incubated for 24 hours. The Biological Reactivity Degree (GR) index, which is the area not stained by the vital dye, is were observed macro and microscopically. Samples were tested in quadruplicates on separate plates. ThusThereunto, the test result showed that the essential oil was cytotoxic in the cell line studied, and that the future work in pharmacology and toxicology needs to be performed to better standardize the therapeutic dose, which is not cytotoxic as the pure oil behaved.o assess the attitude and practice of dental professionals towards using of advance Radiographic technique.

Occurrence of skeletal malocclusions in Brazilian patients with dentofacial deformities.

In this study, a survey was conducted on the occurrence of skeletal malocclusions presented by patients of the Center for Research and Treatment of Buccofacial Deformities (CEDEFACE) in the city of Araraquara, SP, Brazil. The clinical charts of 381 patients with dentoskeletal deformities, who underwent combined orthodontic-surgical treatment in the period between 2000 and 2006, were reviewed. After sample selection (convenience method), based on the data of the pre- and post-surgical documentation, the number of patients was reduced to 171. For classification of the survey, the anteroposterior discrepancy (Class I, II and III), race, age, gender, absence or presence of asymmetry, vertical maxillary excess and maxillary biprotrusion were considered, in addition to determining in which bony base the surgical procedure was performed. Patients' documentations were analyzed by one examiner previously calibrated by repetition of the process until the method was considered adequate (intraclass correlation coefficient > 0.94). Patients' mean age was 23.59 (SD 6.93) years, the majority (102 patients) were women, and Caucasians (160 patients). Class III malocclusion was the most prevalent (81 patients). Asymmetry, vertical maxillary excess and biprotrusion were present in 54, 33, and 7 patients, respectively. The majority of surgeries for correction of dentoskeletal deformities were combined, involving the maxilla and mandible. In conclusion, Class III was the most prevalent skeletal deformity and Class I was the least prevalent; in general, the prevalence of skeletal deformities was higher in women; in the majority of patients with skeletal malocclusions there was a combination of maxillary and mandibular problems, which interferes directly in the decision regarding the most adequate treatment plan, and a higher incidence of asymmetry was observed in skeletal Class III; vertical excess occurred in a similar manner in Class II and III and there was a low incidence of biprotrusion among the malocclusions evaluated.

Insertion angle of orthodontic mini-implants and their biomechanical performance: finite element analysis / Ângulo de inserção de mini-implantes ortodônticos e seu desempenho biomecânico: análise de elementos finitos

Objective: The aim of this study was to assess the stresses and strains generated after the application of two types of forces (traction of 200 gf and torsion of 20 N.cm) in two types of orthodontic mini-implants inserted at different (45° and 90° to the cortical bone) angles. Material and method: three-dimensional models of two brands of mini-implant (SIN - Sao Paulo, Brazil, and RMO - South Korea) were exported and analyzed by finite element analysis (FEA). Analyses were performed on simulations of cortical bone, cancellous bone and the screw. Result: FEA analysis showed that RMO mini-implants had greater elastic deformation when subjected to tensile and torsional forces when compared with SIN mini-implants. For both trademarks and insertion angles tested, there was greater cortical bone deformation, but with the greatest strain located on the mini-implant. Tension on the mini-implant was located in its transmucosal profile region. Conclusion: When comparing the two brands of mini-implants by FEA, it is fair to conclude that that the larger number of threads and their greater angle of inclination resulted in less resistance to deformation and induced a higher level of tension in the mini-implant and cortical bone when subjected to forces, especially when inserted at an angle of 45º to the cortical bone.

Objetivo: O objetivo deste estudo foi avaliar as tensões e deformações de duas marcas comerciais de mini-implantes ortodônticos geradas após a aplicação de dois tipos de forças (de tração de 200 gf e torção de 20 N.cm) inseridos em duas angulações (45° e 90° em relação ao osso cortical). Material e método: Modelos tridimensionais das duas marcas de mini-implantes (SIN - Sao Paulo, Brasil, e RMO - Coréia do Sul) foram construídos e analisados por análise de elementos finitos (FEA). As análises foram realizadas em simulações no osso cortical, osso esponjoso e no parafuso. Resultado: A análise FEA mostrou que os mini-implantes da marca RMO apresentaram maior deformação elástica quando submetidos à tração e as forças de torção quando comparado aos mini-implantes da marca SIN. Em ambas as marcas testadas, e para os diferentes ângulos de inserção, houve uma maior deformação do osso cortical, com maior tensão localizado no mini-implante. A tensão no mini-implante foi localizado na região do perfil transmucoso. Conclusão: Ao comparar as análises de elementos finitos das duas marcas comerciais de mini-implantes, concluiu-se que um maior número de roscas e maior inclinação resultam em menor resistência à deformação e induzem uma maior tensão no osso cortical quando submetidos à forças de torção e tração, especialmente quando inserido em um ângulo de 45º com o osso cortical.

In vitro evaluation of anti-adherence activity of the Ocimum basilicum l. ( basil) extract and of the Coriandrum sativum l. ( coriander) in acrylic surface of removable orthodontic appliances

AIM: The aim of this study is evaluate the antimicrobial activity of the Ocimum basilicum L. (basil) extract and of the Coriandrum sativum L. (coriander) extract in biofilm of Streptococcus mutans colonized in specimens confectioned in acrylic used to confection removable orthodontic appliances. MATERIAL AND METHODS: To perform this work, specimens were confectioned (spherical discs in sterile acrylic) which were immersed in extracts pure and in series dilutions (1:2 a 1:128) by 24, 48 and 72 hours. For each time of exposition, the disintegration of bacterial films was performed by sonication in saline solution. RESULTS: The results obtained were satisfactory for the extracts studied on the inhibition of biofilm formation. CONCLUSION: We conclude that polyphenols present in the extracts interfered on the glucan synthesis engine, possibly inhibiting the enzymes (glucosyl- and fructosyl transferase) which synthesize the extracellular polymers, also acting as antioxidant, and therewith they presented antimicrobial activity.

Effect of angled installation of orthodontic mini-implants on primary stability

AIM: The aim of this work was evaluate the insertion and removal torque for orthodontic mini-implants inserted in different inclination. Materials and methods: Ten self[drilling mini-implants from the brand SIN (Sistema de Implantes Nacional, São Paulo/SP, Brazil), and the surgical kit for their insertion were used. Two plaques of synthetic bone of 120mm x 170mm x 41,5mm were used (Sawbones, Pacific Research Laboratories Inc, Vashon, Wash), with 1,5mm height, simulating the cortical bone (density 40 pcf) and 40 mm simulating the medullary bone (density 15 pcf). In each block, five areas were demarcated for each mark, totalizing ten areas. The ten mini-implants were inserted by the same operator, previously calibrated; five of them at 900 and five at 600, using the manual key kit. After the insertion of all the mini-implants, the final threading and the reading of insertion torque value were carried out with a manual torque wrench digital Lutron TQ[8800 (Lutron Electronic Enterprise Co., Ltd, Taipei, Taiwan) until the trans-mucosal profile achieve the cortical bone. The maximum insertion torque value was registered in N/cm. After all the implants inserted, the measurement of removal torque was started, performed in the same way of insertion, but in the opposite anticlockwise. The results were submitted to the T test (parametric) and to a Mann-Whitney test (non-parametric). Results: The results demonstrated that the insertion torque was lower than the removal one in both insertion degrees, with statistically significance. Despite insertion torque at 90 degrees had been lightly higher than that inserted at 60 degrees, they were not statistically significant. Conclusion: In view of the results, it was possible conclude that insertion at 60º angulation does not offer advantages to the primary stability for orthodontic mini-implants.

Success rate of immediate nonfunctional loaded single-tooth implants: immediate versus delayed implantation.

PURPOSE: The aim of this study was to evaluate the success rate of maxillary immediate nonfunctional single-tooth loaded implants used into fresh extraction sites (immediate placement condition) or healed ridge (delayed placement condition). MATERIALS AND METHODS: Eighty-two dental implants were placed in the maxilla of 64 consecutive patients from private practice office and from a specialization course in Implantology. Forty-six implants were inserted under immediate placement condition, and 36 were inserted under delayed placement condition. The criteria used to evaluate success rate were those previously described by Albrektsson and Zarb (Int J Prosthodont. 1993;6:95-105), and follow-up period ranged from 18.0 to 39.7 months. RESULTS: Seventy-nine implants fulfilled the success rate criteria (96.3%). Moreover, differences concerning implantation condition were not significant (P = 0.33, Qui-square test): three of the failed implants were from immediate placement group (success rate of 93.5%), and none was from delayed placement group (success rate of 100.0%). CONCLUSION: In the present sample, no statistically significant differences were detected for immediate nonfunctional single-tooth loaded implants under immediate placement condition in comparison with those inserted under delayed placement condition; both protocols had high success rate in maxillary incisors, canines, and premolars areas.

Occurrence of skeletal malocclusions in Brazilian patients with dentofacial deformities

In this study, a survey was conducted on the occurrence of skeletal malocclusions presented by patients of the Center for Research and Treatment of Buccofacial Deformities (CEDEFACE) in the city of Araraquara, SP, Brazil. The clinical charts of 381 patients with dentoskeletal deformities, who underwent combined orthodontic-surgical treatment in the period between 2000 and 2006, were reviewed. After sample selection (convenience method), based on the data of the pre- and post-surgical documentation, the number of patients was reduced to 171. For classification of the survey, the anteroposterior discrepancy (Class I, II and III), race, age, gender, absence or presence of asymmetry, vertical maxillary excess and maxillary biprotrusion were considered, in addition to determining in which bony base the surgical procedure was performed. Patients' documentations were analyzed by one examiner previously calibrated by repetition of the process until the method was considered adequate (intraclass correlation coefficient > 0.94). Patients' mean age was 23.59 (SD 6.93) years, the majority (102 patients) were women, and Caucasians (160 patients). Class III malocclusion was the most prevalent (81 patients). Asymmetry, vertical maxillary excess and biprotrusion were present in 54, 33, and 7 patients, respectively. The majority of surgeries for correction of dentoskeletal deformities were combined, involving the maxilla and mandible. In conclusion, Class III was the most prevalent skeletal deformity and Class I was the least prevalent; in general, the prevalence of skeletal deformities was higher in women; in the majority of patients with skeletal malocclusions there was a combination of maxillary and mandibular problems, which interferes directly in the decision regarding the most adequate treatment plan, and a higher incidence of asymmetry was observed in skeletal Class III; vertical excess occurred in a similar manner in Class II and III and there was a low incidence of biprotrusion among the malocclusions evaluated.

Este estudo avaliou a ocorrência de más oclusões esqueléticas apresentadas pelos pacientes do Centro de Pesquisa e Tratamento das Deformidades Bucofaciais (CEDEFACE), na cidade de Araraquara, SP, Brasil. Foram avaliados prontuários de 381 pacientes com deformidades dentoesqueléticas, que fizeram tratamento combinado ortodôntico-cirúrgico no período entre 2000 e 2006. Após a seleção da amostra (método de conveniência), baseado nos dados da documentação pré e pós-cirúrgica, o número de pacientes foi reduzido para 171. Para classificação do levantamento, considerou-se a discrepância ântero-posterior (Classe I, II e III), raça, idade, gênero, ausência ou presença de assimetria, excesso vertical maxilar e biprotrusão maxilar, além de determinar em qual base óssea o procedimento cirúrgico foi realizado. As documentações dos pacientes foram analisadas por um examinador previamente calibrado pelo processo de repetição até que o método fosse considerado adequado (correlação intraclasse >0,94). A idade média dos pacientes foi de 23,59 anos (DP 6,93), a maioria do gênero feminino (102 pacientes) e leucoderma (160 pacientes). A má oclusão mais prevalente foi a Classe III (81 pacientes). A assimetria, o excesso maxilar vertical e biprotrusão maxilar estavam presentes em 54, 33, e 7 pacientes, respectivamente. Na maioria dos casos, as cirurgias para correção de deformidades dentoesqueléticas foram combinadas, envolvendo os dois maxilares. Com base nos resultados, conclui-se que a Classe III foi a deformidade esquelética mais prevalente e a Classe I a menos prevalente. Em geral, a prevalência de deformidades esqueléticas foi maior entre as mulheres e a maioria dos pacientes apresentou uma combinação de problemas maxilares e mandibulares, o que interfere diretamente na decisão sobre o plano de tratamento mais adequado. Houve uma maior incidência de assimetria na Classe III esquelética; o excesso vertical ocorreu de forma semelhante na Classe II e III e a biprotrusão teve baixa incidência entre as más oclusões avaliadas.

Influência do formato do implante para reabilitação de casos com limitação de espaço mesiodistal / Influence of the format of the implant on rehabilitation in cases of mesio-distal space limitation

Apesar do grande desenvolvimento da implantodontia, seu sucesso depende de um planejamento rigoroso e em casos com limitação de espaço mesiodistal a seleção do formato do implante é muito importante. Objetivo e relato do caso clínico: Este artigo relata o caso clínico de um paciente com agenesia bilateral dos incisivos laterais superiores e ausência de espaço satisfatório no sentido mesiodistal, impedindo a utilização de implantes em proporções convencionais, apesar de ter realizado um tratamento ortodôntico prévio. Após a realização de exames radiográficos e estudo de modelos articulados em articulador semiajustável, optou-se pela realização de dois implantes de corpo único com carga imediata, da marca SIN®-Unident, de diâmetro reduzido para a solução do caso. Considerações finais: O correto planejamento, diagnóstico e execução da técnica apresentou resultado satisfatório imediato e a longo prazo.

Tratamento multidisciplinar: relato de casos clínicos / Multidisciplinary treatment: clinical cases report

Opaciente adulto apresenta maior predisposição para doença periodontal, decorrente, principalmente, do mal controle de placa. Nesses pacientes, a movimentação ortodôntica não é contraindicada, porém, é necessária uma avaliação da condição periodontal para que se possa estabelecer o plano de tratamento apropriado. / Objetivo / Descrever e discutir casos clínicos de indivíduos com necessidade de reabilitação bucal e com periodonto severamente comprometido. / Métodos / Os métodos consistiram no tratamento ortodôntico de dois casos com comprometimento periodontal. Após exames clínicos e radiográficos, os casos foram analisados de forma multidisciplinar, envolvendo as especialidades de Ortodontia, Periodontia e Prótese, com objetivo de proporcionar ao paciente os melhores resultados estético, funcional e estabilidade. O tratamento periodontal consistiu de raspagem supra- e subgengival, previamente ao tratamento ortodôntico, e programa de manutenção periódica trimestral durante toda movimentação ortodôntica. As ativações foram realizadas em intervalos de 45 a 50 dias, com a utilização de forças leves, sendo utilizadas contenções em ambas as arcadas, mesmo após a finalização da reabilitação. / Conclusão / O tratamento reabilitador bucal, quando feito de forma multidisciplinar, traz resultados bastante satisfatórios. A interação da Ortodontia e da Periodontia comprovou que pacientes com periodonto reduzido, porém saudável, podem receber tratamento ortodôntico, desde que as forças não excedam o limite biológico desse.

Uso de um distrator ósseo dentoimplantossuportado, feito a partir de tornos expansores convencionais, na reabilitação oral / Use of a tooth-implant supported bone distractor, made from standard orthodontic expansion-screws, in oral rehabilitation. Case report

A osseodistração é uma realidade clínica disponível já há algumas décadas para a resolução de deficiências ósseas extensas, em casos em que existam dentes ou implantes osseointegrados, porém mal posicionados. O objetivo deste artigo é apresentar um caso clínico na qual uma nova possibilidade de distração óssea, baseada em um distrator dentoimplantossuportado (feito com tornos expansores ortodônticos convencionais), foi utilizada em uma área onde havia necessidade extensa de recuperação estética e de tecido ósseo, aliada ao mau posicionamento dentário e de um implante previamente instalado na região. A técnica utilizada apresentou bons resultados clínicos, associado à sua simplicidade e baixo custo, contribuindo como solução clínica viável para ganho tecidual e otimização estética. Como conclusão tem-se que a técnica sugerida alcançou os resultados esperados, melhorando a posição do implante e o perfil de tecidos duro e mole, permitindo a finalização de sua reabilitação oral.

Osteodistraction is a clinical reality, available in the last decades for the resolution of large bone deficiencies, in cases that there are pre-existing, but misplaced implants and teeth. The aims of thearticle is to present a case report in which a new possibility for bone distraction, based on tooth-implant bone distractors, made from standard orthodontic expansion-screws, was used in an area where there was an extensive need of alveolar bone and aesthetical recovery, allied to teeth and dental implant misplacement. This technique presented good clinical results, associated to effective simplicity and low cost, becoming viable clinical solution for bone tissue augmentation and aesthetical optimization. In resume, it is possible to say that the suggested technique achieved its objectives, improving the hard and soft tissue profile, whilst allowing completion of oral rehabilitation.

Avaliação do comportamento de quatro biomateriais associados a enxertos ósseos autógenos nos procedimentos cirúrgicos de elevação de seio maxilar. Estudo histomorfométrico em humanos / Evaluation of the behavior of 4 different biomaterials associated with autogenous bone graft in the sinus procedures. A histomorphometric study in humans

O objetivo deste trabalho foi estudar, histológica e histométricamente, o comportamento de enxertos ósseos e biomateriais, utilizados para aumento de altura óssea em cavidade sinusal de humanos. Para tanto, foram selecionados 20 pacientes e divididos em quatro grupos que após obtenção do material de enxerto da região de sínfise mentoniana, receberam o seguinte tratamento: Grupo GI, após elevação do assoalho da cavidade sinusal receberam associação de osso autógeno e osso desmineralizado, seco e congelado (DemboneÒ), Grupo GII, receberam após elevação do assoalho da cavidade sinusal associação de osso autógeno e hidroxiapatita (OsteogenÒ), Grupo GIII, receberam associação do osso autógeno e polímero vegetal osteointegrável (Poliquilâ), após elevação da cavidade sinusal e Grupo GIV, após elevação do assoalho do seio maxilar receberam associação do osso autógeno cimento de fosfato de cálcio (Bone Source®). Os pacientes de cada condição experimental permaneceram, durante o período de 10 meses, sem receber carga mastigatória na região citada e após esse período, os pacientes receberam implantes osseointegrados, sendo nesse momento coletado material para exame histológico e histométrico. Os resultados mostraram que houve ganho de massa óssea em todos os grupos, onde o osso novo formado, mostrou características habituais da região. Além disso as substâncias utilizadas apresentaram compatibilidade biológica, pois foram integradas total ou parcialmente aos tecidos ao longo do processo de regeneração óssea. Os resultados histométricos mostraram um índice médio de formação óssea de 60,86% para o Grupo GI, 54,29% para o grupo GII, 38,50% para o grupo GIII e 44,35% para o grupo GIV, verificando-se diferenças entre os grupos de tratamento, no entanto não se pode apontar superioridade de um dos materiais

The aim of this study was to evaluate, by histological and histometric analysis, the behavior of the association of autogenous bone graft with biomaterials used in sinus lift procedures. Out of 20 patients were used in this study, and they were equally distributed among 4 different groups: GI: Autogenous bone graft with DFDBA (Dembone®); GII: Autogenous bone graft with hidroxilapatite (Osteogen)®; GIII: Autogenous bone graft with castor oil polimer (Poliquil®); and GIV: Autogenous bone graft calcium phosphate cement (Bone Source ®).After the subantral augmentation was performed the autogenous bone graft was collected from the chin and associated with the biomaterial, according to the group the patient was previously located, and subantral cavity was filled. The groups were allowed to repair without load during 10 months. After this period biopsies were taken, during implant placement sugery for analysis. The results showed an improvement in bone area in all groups; The biomaterials were total or partial absorved thought out the bone regeneration process. The results of histomorfometrical analysis were GI 60,86%, GII 54,29% ; GIII 38,50% and GIV 44,35%, in although there were differences among the groups none of the materials showed a better result when compared to another one