[Virulence of Sporothrix globosa in murine models]. / Virulencia de Sporothrix globosa en modelos murinos.

INTRODUCTION: The sporothricosis disease is an infection caused by species included in Sporothrix schenkii complex. OBJECTIVE: Verify the virulence of a strain of S. globosa using two different concentrations of inoculum by intraperitoneally and subcutaneously, into a mouse model. MATERIALS AND METHODS: Nonrandomized pilot study, in murine inoculated with a strain of S. globosa (CBS 14.076M) by intraperitoneally and subcutaneously with inoculum concentrations of 0.5 and 4 McFarland. For this purpose 18 rodents CF-1 (ISP, Santiago, Chile) were used. RESULTS: The studied strain did not induce illness or injury on animals, they all survived and neither the tissue culture nor the histopathological analysis showed fungal growth or suggestive infection by organ abnormalities. CONCLUSIONS: The S. globosa strain did not present any virulence enough to cause disease at 0.5 and 4.0 McFarland concentration inoculum when inoculated in both intraperitoneally and subcutaneously, in murine models.

Autopsias realizadas por el Dr. Salvador Allende en el Hospital Carlos van Buren de Valparaíso, Chile / Autopsies performed by Dr. Salvador Allende at Carlos van Buren Hospital of Valparaíso, Chile

Introducción: entre los años 1933 y 1937, el Dr. Salvador Allende trabajó con regularidad en el servicio de Anatomía Patológica del Hospital Carlos van Buren de Valparaíso (HCVB), realizando autopsias en los casos de muertes de causa médica. Materiales y método: se revisaron los libros del servicio de Anatomía Patológica del HCVB del año 1937 y se seleccionaron las autopsias realizadas por el Dr. Allende. Los libros y autopsias de los años 1933 a 1936 no pudieron ser encontrados. Los datos demográficos de los pacientes se traspasaron a una planilla Excel. Los resultados se expresaron en números y porcentajes. Resultados: el Dr. Allende realizó 54 autopsias durante un periodo de 5 meses, lo que correspondió al 12,6% de las realizadas ese año. De los 54 pacientes, 48 (88,8%) correspondieron al sexo masculino, con un promedio de edad de 46 (9-97) años. La mayoría tenían nacionalidad chilena y estaban cesantes. El 53,7% de los casos falleció por una causa infecciosa y las más frecuentes fueron la neumonía y la tuberculosis. La concordancia entre los diagnósticos clínicos y de Anatomía Patológica fue de un 70,3%. Conclusiones: se encontró solo una parte de las autopsias realizadas por el Dr. Allende en el HCVB. La mayoría de los pacientes fallecidos fueron jóvenes del sexo masculino y cesantes. Las principales causas de muerte fueron la neumonía y la tuberculosis. (AU)

Introduction: Dr. Salvador Allende often worked between the years 1933 and 1937 for the pathology department in the Carlos van Buren Hospital, performing autopsies in the medical cause death cases. Materials and Methods: the pathology department books from 1937 were reviewed searching for the autopsies performed by Dr. Allende and those were selected. The books and autopsies of the years 1933-1936 could not be found. The demographic data about patients were transfered into an excel spreadsheet. The results were expressed as numbers and percentages. Results: Dr. Allende performed 54 autopsies over a period of 5 months, which corresponded to 12.6% of all the autopsies performed that year. Of the 54 patients, 48 were male (88.8%), with an average age of 46 (9-97) years. Most of them had Chilean nationality and unemployed. 53.7% of studied cases died by an infectious cause being the most common pneumonia and tuberculosis. The concordance between clinical diagnoses and pathology department were 70.3%. Conclusions: Only a small part of the autopsies performed by Dr. Salvador Allende in HCVB was found. Most deceased patients were young men and unemployed. The main causes of death were pneumonia and tuberculosis.(AU)

Virulencia de Sporothrix globosa en modelos murinos / Virulence of Sporothrix globosa in murine models

Introducción: La esporotricosis es una infección causada por especies pertenecientes al complejo Sporothrix schenckii. Dependiendo de la especie, estos organismos pueden tener virulencia y formas clínicas diferentes. Objetivo: Verificar la virulencia de una cepa de Sporothrix globosa en un modelo murino usando 2 concentraciones de inóculo, en aplicación intraperitoneal o subcutánea. Materiales y métodos: Estudio experimental no aleatorizado en murinos inoculados con una cepa de S. globosa (CBS 14.076 M), por vía intraperitoneal y subcutánea, a 0,5 y 4 de concentración McFarland. Se utilizaron 18 roedores CF-1 (ISP, Santiago, Chile). Resultados: La cepa estudiada no provocó enfermedad ni lesiones, todos los animales sobrevivieron, no hubo desarrollo de hongos en el cultivo de tejidos y el análisis histopatológico de órganos no mostró alteraciones sugerentes de infección. Conclusiones: La cepa de S. globosa estudiada no presentó virulencia en modelos murinos y no provocó enfermedad al ser inoculada a concentración McFarland 0,5 y 4, tanto por vía intraperitoneal como subcutánea.

Introduction: The sporothricosis disease is an infection caused by species included in Sporothrix schenkii complex. Objective: Verify the virulence of a strain of S. globosa using two different concentrations of inoculum by intraperitoneally and subcutaneously, into a mouse model. Materials and methods: Nonrandomized pilot study, in murine inoculated with a strain of S. globosa (CBS 14.076 M) by intraperitoneally and subcutaneously with inoculum concentrations of 0.5 and 4 McFarland. For this purpose 18 rodents CF-1 (ISP, Santiago, Chile) were used. Results: The studied strain did not induce illness or injury on animals, they all survived and neither the tissue culture nor the histopathological analysis showed fungal growth or suggestive infection by organ abnormalities. Conclusions: The S. globosa strain did not present any virulence enough to cause disease at 0.5 and 4.0 McFarland concentration inoculum when inoculated in both intraperitoneally and subcutaneously, in murine models.