RESUMO
Exfoliative toxin B (ETB) encoded by some large plasmids plays a crucial role in epidermolytic diseases caused by Staphylococcus aureus. We have found as yet unknown types of etb gene-positive plasmids isolated from a set of impetigo strains implicated in outbreaks of pemphigus neonatorum in Czech maternity hospitals. Plasmids from the strains of clonal complex CC121 were related to archetypal plasmid pETBTY4. Sharing a 33-kb core sequence including virulence genes for ETB, EDIN C, and lantibiotics, they were assigned to a stand-alone lineage, named pETBTY4-based plasmids. Differing from each other in the content of variable DNA regions, they formed four sequence types. In addition to them, a novel unique plasmid pETB608 isolated from a strain of ST130 was described. Carrying conjugative cluster genes, as well as new variants of etb and edinA genes, pETB608 could be regarded as a source of a new lineage of ETB plasmids. We have designed a helpful detection assay, which facilitates the precise identification of the all described types of ETB plasmids.
Assuntos
Dermotoxinas/genética , Exfoliatinas/genética , Impetigo/microbiologia , Plasmídeos/genética , Staphylococcus aureus/genética , Proteínas de Bactérias/genética , Bacteriocinas/genética , República Tcheca/epidemiologia , DNA Bacteriano/genética , Humanos , Impetigo/epidemiologia , Pênfigo/epidemiologia , Pênfigo/microbiologia , Filogenia , Plasmídeos/isolamento & purificação , Staphylococcus aureus/classificação , Virulência/genética , Sequenciamento Completo do GenomaRESUMO
Exfoliative toxin A (ETA)-coding temperate bacteriophages are leading contributors to the toxic phenotype of impetigo strains of Staphylococcus aureus. Two distinct eta gene-positive bacteriophages isolated from S. aureus strains which recently caused massive outbreaks of pemphigus neonatorum in Czech maternity hospitals were characterized. The phages, designated ÏB166 and ÏB236, were able to transfer the eta gene into a prophageless S. aureus strain which afterwards converted into an ETA producer. Complete phage genome sequences were determined, and a comparative analysis of five designed genomic regions revealed major variances between them. They differed in the genome size, number of open reading frames, genome architecture, and virion protein patterns. Their high mutual sequence similarity was detected only in the terminal regions of the genome. When compared with the so far described eta phage genomes, noticeable differences were found. Thus, both phages represent two new lineages of as yet not characterized bacteriophages of the Siphoviridae family having impact on pathogenicity of impetigo strains of S. aureus.
Assuntos
Vírus de DNA/genética , DNA Viral/química , DNA Viral/genética , Genoma Viral , Fagos de Staphylococcus/classificação , Fagos de Staphylococcus/isolamento & purificação , Staphylococcus aureus/virologia , Análise por Conglomerados , Infecção Hospitalar/epidemiologia , República Tcheca/epidemiologia , Vírus de DNA/isolamento & purificação , Surtos de Doenças , Exfoliatinas/genética , Ordem dos Genes , Transferência Genética Horizontal , Maternidades , Humanos , Impetigo/epidemiologia , Impetigo/microbiologia , Recém-Nascido , Dados de Sequência Molecular , Fases de Leitura Aberta , Filogenia , Polimorfismo de Fragmento de Restrição , Prófagos/classificação , Prófagos/genética , Prófagos/isolamento & purificação , Análise de Sequência de DNA , Homologia de Sequência , Infecções Estafilocócicas/epidemiologia , Infecções Estafilocócicas/microbiologia , Fagos de Staphylococcus/genética , Staphylococcus aureus/isolamento & purificação , Sintenia , Transdução GenéticaRESUMO
One hundred and twenty-seven exfoliative toxin-producing (ET-positive) strains of Staphylococcus aureus collected in 23 Czech and one Slovak maternity hospitals from 1998 to 2011 were genotypically characterized by multilocus sequence typing (MLST), pulsed-field gel electrophoresis (PFGE) profiling, spa gene polymorphism analysis, and ETA-converting prophage carriage, which resulted in the identification of 21 genotypes grouped into 4 clonal complexes (CC). Ninety-one isolates carried the eta gene alone whilst 12 isolates harboured only the etb gene. Two new, to date not defined, spa types (t6644 and t6645) and 2 novel sequence types (ST2194 and ST2195) were identified in the set of strains under study. The predominant CC121 occurred in 13 Czech hospitals. CC15, CC9, and ST88 (CC88) exclusively included eta gene-positive strains while the strains belonging to ST121 harboured the eta and/or etb genes. This study highlights not only significant genomic diversity among impetigo strains and the distribution of major genotypes disseminated in the Czech and Slovak maternity hospitals, but also reveals their impact in epidermolytic infections.
Assuntos
Genes Bacterianos , Maternidades , Impetigo/microbiologia , Staphylococcus aureus/isolamento & purificação , Técnicas de Tipagem Bacteriana/métodos , Sequência de Bases , Portador Sadio/microbiologia , Tchecoslováquia , Eletroforese em Gel de Campo Pulsado , Equipamentos e Provisões Hospitalares/microbiologia , Genótipo , Humanos , Recém-Nascido , Tipagem de Sequências Multilocus , Fenótipo , Polimorfismo Genético , Infecções Estafilocócicas/microbiologia , Staphylococcus aureus/classificação , Staphylococcus aureus/genéticaRESUMO
The aim of this study was to compare the plasmid contents of methicillin-resistant Staphylococcus aureus (MRSA) strains classified into different clonal clusters (CCs). The isolates were collected from 15 Czech hospitals in 2000-2008. Plasmid DNA was detected in 65 (89%) strains, and 33 of them harbored more than one plasmid type. Altogether 24 different types of plasmids were identified, ranging in size from 1.3 to 55 kb. Restriction endonuclease analysis, plasmid elimination, DNA hybridization, and sequencing were used for their further characterization. It has been found that the conjugative, erythromycin resistance and enterotoxin D encoding plasmids are harbored by strains from different CCs. On the other hand, chloramphenicol and tetracycline resistance plasmids, and most of the penicillinase and cryptic plasmids were only detected in certain CCs. Especially, the pUSA300-like plasmids were found exclusively in the USA300 clone strains. The high diversity in plasmid content detected in the study strains implies that plasmids play a major role in evolution of MRSA clonal lineages.
Assuntos
Variação Genética , Staphylococcus aureus Resistente à Meticilina/genética , Plasmídeos/genética , Células Clonais , Farmacorresistência Bacteriana/genética , Genótipo , Dados de Sequência Molecular , Penicilinase/genéticaRESUMO
Given the great biological importance and high diversity of temperate Staphylococcus aureus bacteriophages, a method is needed for the description of their genomic structure. Here we have updated a multiplex PCR strategy for the complex characterization of S. aureus phages of the family Siphoviridae. Based on the comparative genomic analysis of the available phage sequences, a multilocus PCR strategy for typing the major modules of the phage genome was designed. The genomic modules were classified on the basis of the genes for integrase (10 types), anti-repressor (five types), replication proteins polA, dnaC and dnaD (four types), dUTPase (four types), portal protein (eight types), tail appendices (four types) and endolysin (four types) corresponding to the integrase locus, lysogeny control region, and modules for DNA replication, transcription regulation, packaging, tail appendices and lysis respectively. The nine PCR assays designed for the above sequences were shown to be capable to identify the bacteriophage gene pool present both in the phage and bacterial genomes and their extensive mosaic structure. The established multiplex PCR-based multilocus diagnostic scheme is convenient for rapid and reliable phage and prophage classification and for the study of bacteriophage evolution.
Assuntos
Genoma Viral , Siphoviridae/genética , Staphylococcus aureus/virologia , Hibridização Genômica Comparativa , DNA Viral/genética , Tipagem de Sequências Multilocus , Reação em Cadeia da Polimerase/métodos , Prófagos/classificação , Prófagos/genética , Siphoviridae/classificação , Staphylococcus aureus/genética , Proteínas Virais/genéticaRESUMO
Bacteriophages of the significant veterinary pathogen Staphylococcus pseudintermedius are rarely described morphologically and genomically in detail, and mostly include phages of the Siphoviridae family. There is currently no taxonomical classification for phages of this bacterial species. Here we describe a new phage designated vB_SpsS_QT1, which is related to phage 2638A originally described as a Staphylococcus aureus phage. Propagating strain S. aureus 2854 of the latter was reclassified by rpoB gene sequencing as S. pseudintermedius 2854 in this work. Both phages have a narrow but different host range determined on 54 strains. Morphologically, both of them belong to the family Siphoviridae, share the B1 morphotype, and differ from other staphylococcal phage genera by a single long fibre at the terminus of the tail. The complete genome of phage vB_SpsS_QT1 was sequenced with the IonTorrent platform and expertly annotated. Its linear genome with cohesive ends is 43,029 bp long and encodes 60 predicted genes with the typical modular structure of staphylococcal siphophages. A global alignment found the genomes of vB_SpsS_QT1 and 2638A to share 84% nucleotide identity, but they have no significant similarity of nucleotide sequences with other phage genomes available in public databases. Based on the morphological, phylogenetic, and genomic analyses, a novel genus Fibralongavirus in the family Siphoviridae is described with phage species vB_SpsS_QT1 and 2638A.
Assuntos
Siphoviridae/classificação , Staphylococcus/virologia , Genes Virais , Genoma Viral , Genômica/métodos , Especificidade de Hospedeiro , Filogenia , Siphoviridae/ultraestrutura , Vírion/ultraestrutura , Replicação ViralRESUMO
Lytic bacteriophages are valuable therapeutic agents against bacterial infections. There is continual effort to obtain new phages to increase the effectivity of phage preparations against emerging phage-resistant strains. Here we described the genomic diversity of spontaneous host-range mutants of kayvirus 812. Five mutant phages were isolated as rare plaques on phage-resistant Staphylococcus aureus strains. The host range of phage 812-derived mutants was 42% higher than the wild type, determined on a set of 186 methicillin-resistant S. aureus strains representing the globally circulating human and livestock-associated clones. Comparative genomics revealed that single-nucleotide polymorphisms from the parental phage 812 population were fixed in next-step mutants, mostly in genes for tail and baseplate components, and the acquired point mutations led to diverse receptor binding proteins in the phage mutants. Numerous genome changes associated with rearrangements between direct repeat motifs or intron loss were found. Alterations occurred in host-takeover and terminal genomic regions or the endolysin gene of mutants that exhibited the highest lytic activity, which implied various mechanisms of overcoming bacterial resistance. The genomic data revealed that Kayvirus spontaneous mutants are free from undesirable genes and their lytic properties proved their suitability for rapidly updating phage therapeutics.
Assuntos
Bacteriófagos/genética , Meticilina/farmacologia , Mutação , Staphylococcus aureus/crescimento & desenvolvimento , Composição de Bases , Farmacorresistência Bacteriana , Tamanho do Genoma , Genoma Viral , Genômica , Polimorfismo de Nucleotídeo Único , Staphylococcus aureus/virologiaRESUMO
Twenty-eight enterotoxin H-positive Staphylococcus aureus strains isolated from food samples collected in eleven districts of the Czech Republic between 2000 and 2005 were genotypically characterized by pulsed-field gel electrophoresis (PFGE) profiling, spa gene polymorphism analysis, enterobacterial repetitive intergenic consensus sequence-based PCR (ERIC-PCR) fingerprinting and prophage carriage detection. These strains accounted for about 21% of the food-derived, staphylococcal enterotoxin (SE)-positive isolates. One strain, detected in feta cheese, was implicated in a case of enterotoxinosis. Sixteen of the twenty-eight isolates carried the seh gene alone. The remaining twelve strains harbored the seh gene in combination with other enterotoxin genes, most often the seg and sei genes, followed by the sea, seb, sec and sed genes. Comparison of various genomic profiles resulted in the determination of twenty genotypes designated G-1 to G-20. Two new, to date not defined, spa types (t2000 and t2002) were identified in one strain isolated from raw meat and two strains obtained from prepacked pizza. Evidence has been given that the seh-positive S. aureus isolates from foodstuffs did not originate from a single source or a common ancestor.
Assuntos
Enterotoxinas/genética , Contaminação de Alimentos/análise , Microbiologia de Alimentos , Staphylococcus aureus/genética , República Tcheca , Impressões Digitais de DNA , DNA Bacteriano/análise , Eletroforese em Gel de Campo Pulsado , Enterotoxinas/classificação , Enterotoxinas/isolamento & purificação , Genótipo , Humanos , Polimorfismo Genético , Intoxicação Alimentar Estafilocócica/microbiologia , Intoxicação Alimentar Estafilocócica/prevenção & controle , Staphylococcus aureus/classificação , Staphylococcus aureus/isolamento & purificaçãoRESUMO
The genus Macrococcus is a close relative of the genus Staphylococcus. Whilst staphylococci are widespread as human pathogens, macrococci have not yet been reported from human clinical specimens. Here we investigated Gram-positive and catalase-positive cocci recovered from human clinical material and identified as Macrococcus sp. by a polyphasic taxonomic approach and by comparative genomics. Relevant phenotypic, genotypic and chemotaxonomic methods divided the analyzed strains into two separate clusters within the genus Macrococcus. Comparative genomics of four representative strains revealed enormous genome structural plasticity among the studied isolates. We hypothesize that high genomic variability is due to the presence of a com operon, which plays a key role in the natural transformation of bacilli and streptococci. The possible uptake of exogenous DNA by macrococci can contribute to a different mechanism of evolution from staphylococci, where phage-mediated horizontal gene transfer predominates. The described macrococcal genomes harbor novel plasmids, genomic islands and islets, as well as prophages. Capsule gene clusters, intracellular protease, and a fibronectin-binding protein enabling opportunistic pathogenesis were found in all four strains. Furthermore, the presence of a CRISPR-Cas system with 90 spacers in one of the sequenced genomes corresponds with the need to limit the burden of foreign DNA. The highly dynamic genomes could serve as a platform for the exchange of virulence and resistance factors, as was described for the methicillin resistance gene, which was found on the novel composite SCCmec-like element containing a unique mec gene complex that is considered to be one of the missing links in SCC evolution. The phenotypic, genotypic, chemotaxonomic and genomic results demonstrated that the analyzed strains represent one novel subspecies and three novel species of the genus Macrococcus, for which the names Macrococcus caseolyticus subsp. hominis subsp. nov. (type strain CCM 7927T = DSM 103682T), Macrococcus goetzii sp. nov. (type strain CCM 4927T = DSM 103683T), Macrococcus epidermidis sp. nov. (type strain CCM 7099T = DSM 103681T), and Macrococcus bohemicus sp. nov. (type strain CCM 7100T = DSM 103680T) are proposed. Moreover, a formal description of Macrococcus caseolyticus subsp. caseolyticus subsp. nov. and an emended description of the genus Macrococcus are provided.
RESUMO
Between July 1, 2002 and December 31, 2003, rectal swabs from both hospitalized patients and community subjects in the Czech Republic were taken to ascertain the prevalence of vancomycin-resistant enterococci (VRE). The swabs were used for isolating and identifying enterococci and their susceptibility to antibiotics. Vancomycin resistance phenotypes were verified by PCR detection of vanA, vanB, vanC1 and vanC2 genes. A molecular biology analysis was performed in Enterococcus faecium VanA strains. During the observed period, 2691 rectal swabs from the hospitalized patients and 6529 rectal swabs from the subjects in community setting were examined. In total, 31 VRE of hospital origin and 13 community-population strains were isolated. The prevalence of VRE in the gastrointestinal tract was 1.9% in the hospitalized patients and 0.4% in the community subjects. The prevailing strains were Enterococcus faecium VanA (61.3%) in the VRE of hospital origin and Enterococcus gallinarum VanC (46.2%) in the community VRE. Mutual comparison between the hospital and community Enterococcus faecium VanA strains showed no similarity.
Assuntos
Infecção Hospitalar/microbiologia , Enterococcus faecalis/efeitos dos fármacos , Enterococcus faecium/efeitos dos fármacos , Infecções por Bactérias Gram-Positivas/microbiologia , Resistência a Vancomicina , Infecção Hospitalar/epidemiologia , República Tcheca/epidemiologia , Enterococcus faecalis/isolamento & purificação , Enterococcus faecium/isolamento & purificação , Infecções por Bactérias Gram-Positivas/epidemiologia , Humanos , Testes de Sensibilidade Microbiana , Prevalência , Características de ResidênciaRESUMO
Staphylococcus aureus is one of the most frequent pathogens infecting the respiratory tract of patients with cystic fibrosis (CF). This study was the first to examine S. aureus isolates from CF patients in the Czech Republic. Among 100 S. aureus isolates from 92 of 107 observed patients, we found a high prevalence of resistance to macrolide-lincosamide-streptogramin B (MLS(B)) antibiotics (56%). More than half of the resistant strains (29 of 56) carried a mutation in the MLS(B) target site. The emergence of MLS(B) resistance and mutations conferring resistance to MLS(B) antibiotics was associated with azithromycin treatment (p=0.000000184 and p=0.000681, respectively). Methicillin resistance was only detected in 3% of isolates and the rate of resistance to other antibiotics did not exceed 12%. The prevalence of small-colony variant (SCV) strains was relatively low (9%) and eight of nine isolates with the SCV phenotype were thymidine dependent. The study population of S. aureus was heterogeneous in structure and both the most prevalent community-associated and hospital-acquired clonal lineages were represented. Of the virulence genes, enterotoxin genes seg (n=52), sei (n=49), and sec (n=16) were the most frequently detected among the isolates. The PVL genes (lukS-PV and lukF-PV) have not been revealed in any of the isolates.
Assuntos
Antibacterianos/farmacologia , Antibacterianos/uso terapêutico , Azitromicina/uso terapêutico , Fibrose Cística/microbiologia , Farmacorresistência Bacteriana/genética , Ribossomos/genética , Staphylococcus aureus/efeitos dos fármacos , Staphylococcus aureus/genética , Infecção Hospitalar/microbiologia , República Tcheca , Humanos , Assistência de Longa Duração , Staphylococcus aureus Resistente à Meticilina/efeitos dos fármacos , Testes de Sensibilidade Microbiana , Mutação/genética , Infecções Estafilocócicas/tratamento farmacológico , Infecções Estafilocócicas/microbiologia , Timidina/genéticaRESUMO
Staphylococcus aureus is a serious human and veterinary pathogen in which new strains with increasing virulence and antimicrobial resistance occur due to acquiring new genes by horizontal transfer. It is generally accepted that temperate bacteriophages play a major role in gene transfer. In this study, we proved the presence of various bacterial genes of the S. aureus COL strain directly within the phage particles via qPCR and quantified their packaging frequency. Non-parametric statistical analysis showed that transducing bacteriophages φ11, φ80 and φ80α of serogroup B, in contrast to serogroup A bacteriophage φ81, efficiently package selected chromosomal genes localized in 4 various loci of the chromosome and 8 genes carried on variable elements, such as staphylococcal cassette chromosome SCCmec, staphylococcal pathogenicity island SaPI1, genomic islands vSaα and vSaß, and plasmids with various frequency. Bacterial gene copy number per ng of DNA isolated from phage particles ranged between 1.05 × 10(2) for the tetK plasmid gene and 3.86 × 10(5) for the SaPI1 integrase gene. The new and crucial finding that serogroup B bacteriophages can package concurrently ccrA1 (1.16 × 10(4)) and mecA (1.26 × 10(4)) located at SCCmec type I into their capsids indicates that generalized transduction plays an important role in the evolution and emergence of new methicillin-resistant clones.
Assuntos
Bacteriófagos/genética , Cromossomos Bacterianos/genética , Genes Bacterianos , Sequências Repetitivas Dispersas , Staphylococcus aureus/virologia , Proteínas de Bactérias/genética , Bacteriófagos/metabolismo , Clonagem Molecular , DNA Bacteriano/genética , Frequência do Gene , Transferência Genética Horizontal , Loci Gênicos , Resistência a Meticilina/genética , Proteínas de Ligação às Penicilinas , Penicilinase/genética , Plasmídeos/genética , Reação em Cadeia da Polimerase , Análise de Sequência de DNA , Staphylococcus aureus/genética , Staphylococcus aureus/fisiologia , Montagem de VírusRESUMO
Thirteen coagulase-negative, oxidase-negative, and novobiocin-susceptible staphylococci were isolated from human clinical specimens. The isolates were differentiated from known staphylococcal species on the basis of 16S rRNA, hsp60, rpoB, dnaJ, tuf, and gap gene sequencing, automated ribotyping, (GTG)5-PCR fingerprinting, and MALDI-TOF MS analysis. Phylogenetic analysis based on the 16S rRNA gene sequence indicated phylogenetic relatedness of the analyzed strains to Staphylococcus haemolyticus, Staphylococcus hominis, Staphylococcus devriesei, and Staphylococcus lugdunensis. DNA-DNA hybridization experiments between representative strains CCM 8418(T), CCM 8421(T), and the closest phylogenetic neighbors confirmed that the isolates represent novel Staphylococcus species, for which the name Staphylococcus petrasii sp. nov. is proposed. Genotypic and phenotypic analyses unambiguously split the strains into two closely related subclusters. Based on the results, two novel subspecies S. petrasii subsp. petrasii subsp. nov. and S. petrasii subsp. croceilyticus subsp. nov. are proposed, with type strains CCM 8418(T) (=CCUG 62727(T)) and CCM 8421(T) (=CCUG 62728(T)), respectively.
Assuntos
Orelha/microbiologia , Infecções Estafilocócicas/microbiologia , Staphylococcus/classificação , Staphylococcus/isolamento & purificação , Antibacterianos/metabolismo , Proteínas de Bactérias/genética , Técnicas de Tipagem Bacteriana , Análise por Conglomerados , Coagulase/metabolismo , DNA Bacteriano/química , DNA Bacteriano/genética , DNA Ribossômico/química , DNA Ribossômico/genética , Humanos , Dados de Sequência Molecular , Novobiocina/metabolismo , Hibridização de Ácido Nucleico , Oxirredutases/metabolismo , Filogenia , RNA Ribossômico 16S/genética , Análise de Sequência de DNA , Staphylococcus/genética , Staphylococcus/metabolismoRESUMO
The epidemic community-associated methicillin-resistant clone Staphylococcus aureus USA300 is a major source of skin and soft tissue infections and involves strains with a diverse set of resistance genes. In this study, we report efficient transduction of penicillinase and tetracycline resistance plasmids by bacteriophages φ80α and φJB between clinical isolates belonging to the USA300 clone. High transduction frequencies (10(-5) - 10(-6) CFU/PFU) were observed using phages propagated on donor strains as well as prophages induced from donors by ultraviolet light. Quantitative real-time PCR was employed to detect penicillinase plasmids in transducing phage particles and determine the ratio of transducing particles in phage lysates to infectious phage particles (determined as approximately 1 : 1700). Successful transfer of plasmids between strains in USA300 clone proves transduction is an effective mechanism for spreading plasmids within the clone. Such events contribute to its evolution and to emergence of new multiple drug-resistant strains of this successful clone.
Assuntos
Bacteriófagos/genética , Farmacorresistência Bacteriana , Transferência Genética Horizontal , Staphylococcus aureus Resistente à Meticilina/genética , Staphylococcus aureus Resistente à Meticilina/virologia , Plasmídeos , Transdução Genética , Humanos , Staphylococcus aureus Resistente à Meticilina/isolamento & purificação , Prófagos/genética , Infecções Estafilocócicas/microbiologiaRESUMO
We have isolated and characterized two distinct types of exfoliative toxin A (ETA)-converting bacteriophages originating from Staphylococcus aureus strains responsible for massive outbreaks of pemphigus neonatorum in the Czech Republic. Three induced phages designated as ph iB531, phi B557 and phi B122 were found to be capable of transferring the eta gene into the prophageless non-toxigenic S. aureus strain and converting it into an ETA producer. Comparisons of the phage sequences derived from 12 selected genes and 2 genomic segments (polymorphic P2 and conserved C4) revealed that phi B531 and phi B557 were identical each other, but phi B122 differed from them in 5 gene sequences, the xis gene content and the virion protein profile. Thus, phi B122 represents a new type of still undescribed ETA-converting phage. This study highlights not only the conclusive genomic diversity of eta gene-positive phages, but also their virulence implications in impetigo S. aureus strains.
Assuntos
Exfoliatinas/genética , Variação Genética , Pênfigo/microbiologia , Infecções Estafilocócicas/microbiologia , Fagos de Staphylococcus/genética , Fagos de Staphylococcus/isolamento & purificação , Staphylococcus aureus/virologia , República Tcheca/epidemiologia , Surtos de Doenças , Exfoliatinas/metabolismo , Transferência Genética Horizontal , Humanos , Dados de Sequência Molecular , Pênfigo/epidemiologia , Infecções Estafilocócicas/epidemiologia , Fagos de Staphylococcus/metabolismo , Staphylococcus aureus/genética , Staphylococcus aureus/metabolismoRESUMO
The exfoliative toxin A (ETA) is encoded by the gene located on Staphylococcus aureus prophages. We have developed a single-reaction multiplex polymerase chain reaction (PCR) assay for rapid and specific detection of various phiETA prophages of serogroup B responsible for dissemination of eta gene and ETA production in clinical strains. This PCR strategy enabled to classify the ETA-positive strains into 6 groups designated ETA-B1, ETA-B2, ETA-B3, ETA-B4, ETA-B5, and ETA-B6. The method was tested on a diverse set of 101 ETA and/or ETB-positive S. aureus strains isolated in 22 Czech maternity hospitals and 1 Slovak maternity hospital between 1998 and 2009. This novel PCR strategy is reliable in the rapid identification of yet undescribed ETA-converting B prophages and differentiation of the closely related ETA-positive strains, and it is a convenient tool for hospital epidermolytic infection control.
Assuntos
Exfoliatinas/genética , Reação em Cadeia da Polimerase/métodos , Prófagos/genética , Infecções Estafilocócicas/microbiologia , Staphylococcus aureus/classificação , Técnicas de Tipagem Bacteriana/métodos , Tchecoslováquia , Feminino , Maternidades , Humanos , Recém-Nascido , Pênfigo/microbiologia , Gravidez , Pele/microbiologia , Staphylococcus aureus/genéticaRESUMO
Phage 812 is a polyvalent phage with a very broad host range in the genus Staphylococcus, which makes it a suitable candidate for phage therapy of staphylococcal infections. This proteomic study, combining the results of both 1-DE and 2-DE followed by PMF, led to the identification of 24 virion proteins. Twenty new proteins, not yet identified by proteome analysis of closely related staphylococcal phages K and G1 were identified using this approach. Fifteen proteins were assigned unambiguously to the head-tail genome module; the remaining nine proteins are encoded by genes of the left or right arms of the phage genome. As expected, the most abundant proteins in the electrophoretic patterns are the major capsid protein, the major tail sheath protein and proteins identical to ORF 50 and ORF 95 of phage K, although their function is only putative. Identification of these 20 new proteins contributes substantially to a detailed characterization of phage virions, knowledge of which is necessary for rational phage therapy.
Assuntos
Proteoma/metabolismo , Fagos de Staphylococcus/metabolismo , Proteínas Virais/metabolismo , Eletroforese em Gel de Poliacrilamida , Espectrometria de Massas por Ionização e Dessorção a Laser Assistida por MatrizRESUMO
Genotyping based on pulsed-field gel electrophoresis (PFGE), PCR ribotyping, enterobacterial repetitive intergenic consensus motif (ERIC2)-PCR, and prophage content analysis was carried out in 45 toxic shock syndrome toxin-1 (TSST-1)-positive Staphylococcus aureus strains isolated in 19 cities of the Czech Republic between 1986 and 2004. Twenty-four wild-type TSST-1-positive isolates of which 18 strains were the causative agents of toxic shock syndrome (TSS) had the same genotype profiles as the type strain FRI 1169 and were classified into genotype T1. The remaining 21 strains of which 12 caused TSS were distinct from one another and differed from the T1 strains mainly in PFGE pattern and also in one or more other genetic characteristics. While the strains of genotype T1 are most prevalent in the Czech population and account for the majority of TSS cases, TSS-related S. aureus strains of different genotypes were also found and cannot be excluded as the causative agents of TSS cases in this country.
Assuntos
Toxinas Bacterianas/biossíntese , Enterotoxinas/biossíntese , Choque Séptico/microbiologia , Infecções Estafilocócicas/microbiologia , Staphylococcus aureus/genética , Superantígenos/biossíntese , Toxinas Bacterianas/genética , República Tcheca , Impressões Digitais de DNA , DNA Bacteriano/química , DNA Bacteriano/genética , DNA Intergênico/química , DNA Intergênico/genética , Enterotoxinas/genética , Genótipo , Humanos , Masculino , Reação em Cadeia da Polimerase , Staphylococcus aureus/isolamento & purificação , Staphylococcus aureus/metabolismo , Superantígenos/genéticaRESUMO
Eight coagulase-negative, oxidase-negative and novobiocin-susceptible staphylococcal strains were isolated from the gastrointestinal tracts of South American squirrel monkeys (Saimiri sciureus L.). These strains were differentiated from known staphylococcal species on the basis of 16S rRNA gene and hsp60 gene sequencing, and from the most closely related species by using DNA-DNA hybridization, ribotyping, whole-cell protein profiles and biotyping. Phylogenetic analysis based on 16S rRNA gene sequences confirmed that these strains are members of the Staphylococcus aureus species group (99% similarity) but are biochemically similar to Staphylococcus piscifermentans, from which they can be phenotypically distinguished by resistance to polymyxin B, acid production from D-mannitol, the inability to hydrolyse aesculin and DNA and the absence of alpha-glucosidase. On the basis of these analyses, a novel species of the genus Staphylococcus is described, for which the name Staphylococcus simiae sp. nov. is proposed, with CCM 7213(T) (=LMG 22723(T)) as the type strain.
Assuntos
Trato Gastrointestinal/microbiologia , Saimiri/microbiologia , Staphylococcus/classificação , Animais , Proteínas de Bactérias/análise , Técnicas de Tipagem Bacteriana , Chaperonina 60/genética , DNA Bacteriano/análise , Genes de RNAr , Dados de Sequência Molecular , Hibridização de Ácido Nucleico , Filogenia , RNA Ribossômico 16S/genética , Ribotipagem , Análise de Sequência de DNA , América do Sul , Staphylococcus/genética , Staphylococcus/isolamento & purificação , Staphylococcus/fisiologiaRESUMO
Genotyping of sixteen exfoliative toxin-producing (ET-positive) strains of Staphylococcus aureus isolated in maternity units of two distant hospitals was accomplished by PFGE, ribotyping, PCR ribotyping, and prophage carriage. Three strains secreted combined ETA + ETB, and the remaining produced ETA and enterotoxin C, or TSST-1. The comparison of various genomic profiles resulted in the identification of nine genotypes. The presence of one prevailing genotype was demonstrated in each hospital. Evidence was given that the outbreak-related ET-positive strains causing the skin disease pemphigus neonatorum disseminated in both the hospitals did not originate from a single source or a common ancestor.