Detalhe da pesquisa
1.
The calcium-binding photoprotein clytin II: Expression of the preferred human codon-optimized clytin II gene in Chinese hamster ovary-K1 cells and its use in the G-protein-coupled receptor assays.
Protein Expr Purif
; 220: 106481, 2024 Aug.
Artigo
em Inglês
| MEDLINE | ID: mdl-38583788
2.
Expression, purification, and characterization of recombinant apoPholasin.
Protein Expr Purif
; 171: 105615, 2020 07.
Artigo
em Inglês
| MEDLINE | ID: mdl-32114101
3.
A fusion protein of the synthetic IgG-binding domain and aequorin: Expression and purification from E. coli cells and its application.
Protein Expr Purif
; 137: 58-63, 2017 Sep.
Artigo
em Inglês
| MEDLINE | ID: mdl-28668497
4.
Identification of amino acid residues responsible for high initial luminescence intensity in a calcium-binding photoprotein, clytin-II.
Biochem Biophys Res Commun
; 469(2): 300-5, 2016 Jan 08.
Artigo
em Inglês
| MEDLINE | ID: mdl-26626073
5.
Codon optimization of genes for efficient protein expression in mammalian cells by selection of only preferred human codons.
Protein Expr Purif
; 109: 47-54, 2015 May.
Artigo
em Inglês
| MEDLINE | ID: mdl-25665506
6.
Unconventional secretion of the mutated 19 kDa protein of Oplophorus luciferase (nanoKAZ) in mammalian cells.
Biochem Biophys Res Commun
; 450(4): 1313-9, 2014 Aug 08.
Artigo
em Inglês
| MEDLINE | ID: mdl-25019994
7.
Luminescence enhancement of the catalytic 19 kDa protein (KAZ) of Oplophorus luciferase by three amino acid substitutions.
Biochem Biophys Res Commun
; 445(1): 157-62, 2014 Feb 28.
Artigo
em Inglês
| MEDLINE | ID: mdl-24491536
8.
C6-Deoxy coelenterazine analogues as an efficient substrate for glow luminescence reaction of nanoKAZ: the mutated catalytic 19 kDa component of Oplophorus luciferase.
Biochem Biophys Res Commun
; 437(1): 23-8, 2013 Jul 19.
Artigo
em Inglês
| MEDLINE | ID: mdl-23792095
9.
Expression, purification and luminescence properties of coelenterazine-utilizing luciferases from Renilla, Oplophorus and Gaussia: comparison of substrate specificity for C2-modified coelenterazines.
Protein Expr Purif
; 88(1): 150-6, 2013 Mar.
Artigo
em Inglês
| MEDLINE | ID: mdl-23274053
10.
Reverse mutants of the catalytic 19 kDa mutant protein (nanoKAZ/nanoLuc) from Oplophorus luciferase with coelenterazine as preferred substrate.
PLoS One
; 17(9): e0272992, 2022.
Artigo
em Inglês
| MEDLINE | ID: mdl-36129943
11.
Recombinant Gaussia luciferase with a reactive cysteine residue for chemical conjugation: expression, purification and its application for bioluminescent immunoassays.
Biochem Biophys Res Commun
; 410(4): 792-7, 2011 Jul 15.
Artigo
em Inglês
| MEDLINE | ID: mdl-21703234
12.
Expression and characterization of EF-hand I loop mutants of aequorin replaced with other loop sequences of Ca2+-binding proteins: an approach to studying the EF-hand motif of proteins.
J Biochem
; 160(1): 59-68, 2016 Jul.
Artigo
em Inglês
| MEDLINE | ID: mdl-26896488
13.
Concise Synthesis of v-Coelenterazines.
Org Lett
; 17(15): 3888-91, 2015 Aug 07.
Artigo
em Inglês
| MEDLINE | ID: mdl-26194904
14.
A Novel Catalytic Function of Synthetic IgG-Binding Domain (Z Domain) from Staphylococcal Protein A: Light Emission with Coelenterazine.
Photochem Photobiol
; 90(1): 137-44, 2014 01.
Artigo
em Inglês
| MEDLINE | ID: mdl-24138575