Negative effects of winter and spring warming on the regeneration of forest spring geophytes.

The climate is changing rapidly, provoking species to shift their ranges poleward and upslope. We currently lack a mechanistic understanding of the effect of warmer temperatures on plants, especially for seasonally distinct patterns. Spring geophytes are emblematic forest plants that have a short aboveground lifecycle in the first half of the year and are thus particularly sensitive to winter and spring warming. We set up a warming experiment with separate and combined winter and spring warming on seedlings of three European spring geophytes: Anemone nemorosa, Hyacinthoides non-scripta and Ornithogalum pyrenaicum. Seedling emergence and plant height were recorded at the end of winter and spring treatment, when also biomass of the root, shoot and storage organ was determined. We found negative effects of combined winter and spring warming on seedling emergence. The weight of the storage organ proved to be the best indicator of seedling performance and was negatively affected by separate winter warming in Anemone and by spring warming in Hyacinthoides. Successful seedling emergence was jeopardized by the absence of a cold period, while seedling performance seemed to be negatively influenced directly by higher temperatures through a phenological shift. Our findings confirm that warmer winter and spring temperatures could hamper regeneration of spring geophytes.

Different effects of warming treatments in forests versus hedgerows on the understorey plant Geum urbanum.

The effectiveness of hedgerows as functional corridors in the face of climate warming has been little researched. Here we investigated the effects of warming temperatures on plant performance and population growth of Geum urbanum in forests versus hedgerows in two European temperate regions. Adult individuals were transplanted in three forest-hedgerow pairs in each of two different latitudes, and an experimental warming treatment using open-top chambers was used in a full factorial design. Plant performance was analysed using mixed models and population performance was analysed using Integral Projection Models and elasticity analyses. Temperature increases due to open-top chamber installation were higher in forests than in hedgerows. In forests, the warming treatment had a significant negative effect on the population growth rate of G. urbanum. In contrast, no significant effect of the warming treatment on population dynamics was detected in hedgerows. Overall, the highest population growth rates were found in the forest control sites, which was driven by a higher fecundity rather than a higher survival probability. Effects of warming treatments on G. urbanum population growth rates differed between forests and hedgerows. In forests, warming treatments negatively affected population growth, but not in hedgerows. This could be a consequence of the overall lower warming achieved in hedgerows. We conclude that maintenance of cooler forest microclimates coul, at least temporarily, moderate the species response to climate warming.

Initial oak regeneration responses to experimental warming along microclimatic and macroclimatic gradients.

Quercus spp. are one of the most important tree genera in temperate deciduous forests in terms of biodiversity, economic and cultural perspectives. However, natural regeneration of oaks, depending on specific environmental conditions, is still not sufficiently understood. Oak regeneration dynamics are impacted by climate change, but these climate impacts will depend on local forest management and light and temperature conditions. Here, we studied germination, survival and seedling performance (i.e. aboveground biomass, height, root collar diameter and specific leaf area) of four oak species (Q. cerris, Q. ilex, Q. robur and Q. petraea). Acorns were sown across a wide latitudinal gradient, from Italy to Sweden, and across several microclimatic gradients located within and beyond the species' natural ranges. Microclimatic gradients were applied in terms of forest structure, distance to the forest edge and experimental warming. We found strong interactions between species and latitude, as well as between microclimate and latitude or species. The species thus reacted differently to local and regional changes in light and temperature ; in southern regions the temperate Q. robur and Q. petraea performed best in plots with a complex structure, whereas the Mediterranean Q. ilex and Q. cerris performed better in simply structured forests with a reduced microclimatic buffering capacity. The experimental warming treatment only enhanced height and aboveground biomass of Mediterranean species. Our results show that local microclimatic gradients play a key role in the initial stages of oak regeneration; however, one needs to consider the species-specific responses to forest structure and the macroclimatic context.