Amustaline (S-303) treatment inactivates high levels of Chikungunya virus in red-blood-cell components.

BACKGROUND AND OBJECTIVES: Chikungunya virus (CHIKV) infections have been reported in all continents, and the potential risk for CHIKV transfusion-transmitted infections (TTIs) was demonstrated by the detection of CHIKV RNA-positive donations in several countries. TTIs can be reduced by pathogen inactivation (PI) of blood products. In this study, we evaluated the efficacy of amustaline and glutathione (S-303/GSH) to inactivate CHIKV in red-blood-cell concentrates (RBCs). MATERIAL AND METHODS: Red-blood-cells were spiked with high level of CHIKV. Infectious titres and RNA loads were measured before and after PI treatment. Residual CHIKV infectivity was also assessed after five successive cell culture passages. RESULTS: The mean CHIKV titres in RBCs before inactivation was 5·81 ± 0·18 log10 50% tissue culture infectious dose (TCID50 )/mL, and the mean viral RNA load was 10·49 ± 0·15 log10 genome equivalent (GEq)/mL. No CHIKV TCID was detected after S-303 treatment nor was replicative CHIKV particles and viral RNA present after five cell culture passages of samples obtained immediately after S-303 treatment. CONCLUSION: Chikungunya virus was previously shown to be inactivated by the PI technology using amotosalen and ultraviolet A light for the treatment of plasma and platelets. This new study demonstrates that S-303/GSH can inactivate high titres of CHIKV in RBCs.

Evaluación del proceso de reintegro socio-laboral en personas que han sufrido accidentes del trabajo que fueron atendidas en terapia ocupacional del Hospital del Trabajador Santiago / Evaluation of the social and work reincorporation proces of people who have sufered labour acidents and who were treated at the Ocupational Therapy unit of the Workers Hospital in Santiago

El presente artículo corresponde a una síntesis de los resultados más relevantes obtenidos a partir del estudio denominado Nivel de participación sociolaboral y satisfacción con aspectos de su vida en personas con disminución de su capacidad de ganancia, como consecuencia de un accidente del trabajo, que han recibido apoyos personales y contextuales como parte de un proceso de rehabilitación en el Hospital del Trabajador Santiago. Estudio de Seguimiento 2002-2004.Corresponde a un diseño descriptivo no experimental, de carácter cuantitativo, que incorpora una perspectiva cualitativa. La población estudiada corresponde a 82 personas atendidas en Terapia Ocupacional, evaluadas entre el año 2002 y 2004 con una pérdida de ganancia sobre un 32.5 por ciento. Los resultados muestran que el 89 por ciento de la población ha vuelto a trabajar, encontrándose el 66,7 por ciento plena o medianamente satisfecho con su proceso de reintegro laboral. En el 72.4 por ciento de quienes se reintegran, su jefatura señala que su desempeño se ha mantenido o mejorado. Cabe destacar que el 92 por ciento de trabajadores en los que se efectuó Visitas a Empresas, ya sea para evaluar el puesto de trabajo o efectuar una evaluación en situación real, tiene resultados exitosos, lo que permite avalar técnicas propias del modelo de intervención. Los aspectos relevantes que impactan en la percepción de calidad de vida son las posibilidades para ejercer roles y las interferencias de las secuelas en las relaciones interpersonales.

This item is a summary of the most relevant results obtained from the study called Level of social and labor participation and satisfaction with aspects of life in people with reduced earning capacity as a result of an labor accident, that have received personal and contextual support as part of a rehabilitation process in the Workers’ Hospital of Santiago. Monitoring Survey 2002-2004. It represents a descriptive non-experimental design, quantitative and incorporating a qualitative perspective. The population studied 82people treated in Occupational Therapy, evaluated between 2002 and 2004 with a loss of profit on 32.5 percent. The results show that 89 percent of the population has returned to work, being 66.7 percent fully or fairly satisfied with their job reinstatement process. In 72.4 percent of those returning, their boss pointed out that his performance has been maintained or improved. It is noteworthy that 92 percent of workers that took place in the Company Visits, to assess whether the job position or making a real situation assessment, had successful results, allowing endorse own techniques of the intervention model. Relevant aspects that impact on the perception of quality of life are the chance to practice roles and interference in the aftermath of interpersonal relationships.