Welfare Assessment: Correspondence Analysis of Welfare Score and Hematological and Biochemical Profiles of Dairy Cows in Sardinia, Italy.

The need for animal welfare definition and assessment is increasing worldwide, and several studies have been conducted to help fill the knowledge gaps regarding the welfare of cattle. However, further studies are needed to provide valid synthetized measures for welfare evaluation. The aim of this study was to assess the welfare status of 16 Sardinian dairy cattle farms, based on the developed Animal Welfare and Biosecurity Evaluation checklist (AWB-EF) and the corresponding hematological, biochemical, and electrophoretic profiles of these animals. Considering the AWB-EF as gold standard, blood samples were collected from 230 Holstein breed dairy cattle, aged between 3 and 8 years, out of the periparturient period, and with no clinical signs of specific pathologies. Principal Component (PC) and correlation analyses were performed to simplify phenomena interpretation and assess positive/negative associations. Four PCs were able to explain 76% of the total variability, and six laboratory parameters were strongly associated with the AWB-EF score (Spearman's correlation coefficient ≥ 0.40, p-Value < 0.05), reflecting the real health status of the animals. Given the complexity of animal welfare as a multidimensional concept and the need to include both animal-based and non-based measures in welfare evaluation, the present work represents a sound basis for future evaluation and veterinary health planning.

First Isolation of Brucella canis from a breeding kennel in Italy.

Brucella canis has been isolated for the first time in Italy in a commercial breeding kennel. It was diagnosed after a deep investigation related to the onset of reproductive disorders. Animals were tested with direct and indirect techniques. The agent was first detected in two Chihuahua aborted foetuses by direct culture. Further, it was also isolated from blood samples of dogs hosted in the kennel, which also showed reaction to conventional serological tests (microplate serum agglutination test). The isolates were identified as B. canis by standard microbiological methods and a Bruce­ladder multiplex PCR. To investigate the genomic diversity, whole genome sequencing was used, applying the core genome Multilocus Sequence Typing (cgMLST ). In a first round of serological testing performed on 598 animals, 269 (46.1%) tested positive. In the second round of laboratory testing carried out 4­5 weeks apart, the number of serologically positive dogs was 241 out of 683 tested (35.3%), while the number of dogs positive to isolation was 68 out of 683 tested (10.0%). The PCR showed a lack of sensitivity when compared to direct isolation. The epidemiological investigation did not identify the source of the infection, given the time elapsed from the onset of abortions to the definitive diagnosis of B. canis infection in the kennel. The genomic analyses featured the strains as ST21 and, according to the cgMLST, revealed the presence of a tight cluster with a maximum diversity of four allelic differences. The observed limited genomic variation, largely within the known outbreak cut­offs, suggests that the outbreak herein described was likely caused by a single introduction. Moreover, in a broader scale comparison using the public available genomes, we found that the closest genome, isolated in China, differed by more than 50 alleles making not possible to find out the likely origin of the outbreak. The lack of updated data on B. canis genome sequences in the public databases, together with the limited information retrieved from the epidemiological investigations on the outbreak, hampered identification of the source of B. canis infection.

The national information system for the notification of animal diseases in Italy.

This paper describes the national system for the notification and management of outbreaks of animal diseases in Italy (Sistema Informativo Nazionale Malattie Animali: SIMAN). The main objective of the system is to provide a tool for the management of epidemic emergencies and to fulfil the information obligations towards international organisations, such as the European Commission and World Organisation for Animal Health (Office International des Épizooties: OIE). SIMAN was thus designed to collect all relevant information on outbreaks of animal diseases and to provide a useful tool for the management of activities to be implemented in emergencies. SIMAN is able to collect and report information concerning suspected or confirmed animal outbreaks in a consistent way and allows veterinary services to enter data electronically, instead of using paperwork, within the framework of the process of e-government and dematerialisation of the administrative acts. Data are immediately accessible for local and national authorities. The system provides the relevant national authorities with information relative to the planning of control measures in case epidemic emergencies. SIMAN is part of the e-government process that involves all public administrations of the European Union (EU) and refers to the use of information and communication technologies for the digital processing of documents so as to simplify the system and to make administrative procedures on the Internet much easier to follow.

Prevalence of thermotolerant Campylobacter in broiler flocks and broiler carcasses in Italy.

In accordance with European Union regulations, from 5 February until 15 December 2008, sampling and analysis activities were conducted in Italy to assess the extent of contamination caused by thermotolerant Campylobacter in broiler chickens farmed nationwide. The survey involved 48 poultry slaughterhouses distributed across eleven regions of Italy, where the caeca and carcasses of 393 slaughter batches were sampled. A total of 284 batches (72.3%) gave positive results for Campylobacter spp. as follows: 52.1% were contaminated by C. jejuni, 55.6% by C. coli and 1.1% by C. lari. C. jejuni and C. coli were isolated together in 37 batches (13% of positive results). Campylobacter spp. was isolated only from the caeca in 251 slaughter batches (63.9%) including caecal isolates of C. jejuni (48.2%), C. coli (50.6%), and C. lari (1.2%). Carcasses from 182 batches (46.3%) were contaminated by C. jejuni in 40.7% of cases, C. coli in 57.7% and the absence of C. lari from all batches examined. The contamination level observed in the carcasses ranged between 10 and 1.6 × 10(7) cfu/g.

Implementation of an integrated information system for the management of swine vesicular disease surveillance activities in Italy.

In Europe in the last decade, swine vesicular disease (SVD) outbreaks have been steadily reported in Italy where surveillance and eradication activities are in place. To collect and analyse data gathered during SVD surveillance activities, the Italian reference centre for vesicular diseases (CERVES) has implemented an integrated information system for the management of the national surveillance plan. The system was developed using Web-based technology and open source software. It was designed to gather, integrate and manage data generated by the activities of the Veterinary Services, the laboratory information system, herd and animal registers and the relevant technical and scientific information. A geographic information system provides an interface for the system, which facilitates the planning, implementation and evaluation of disease control actions. Access to the system is feasible through a second level domain.

A Web-based geographic information system for the management of animal disease epidemics.

Outbreaks of highly contagious animal diseases severely affect the global economy, seriously disrupting domestic economies and the international trade of livestock and animal commodities. Italy has a series of contingency plans for the management of outbreaks of all major animal diseases. Disease control measures are mainly based on a traditional approach and eradication policies range from culling and stamping-out to movement restrictions of animals and commodities. Given the marked terrain differences in Italy, the uneven distribution of farms and animal population, the presence of potential disease hotspots constituted by high-density clusters of farms and animals, etc., maps have always been a common working tool for the Italian veterinary services to identify location of premises, define the perimeter of the infected and control zones, etc. The Italian Ministry of Health has requested an integrated system for the management of veterinary emergencies. One of the main features of the system is an interactive, customisable and user-friendly Web-based geographic information system (GIS). The Istituto Zooprofilattico Sperimentale dell'Abruzzo e del Molise 'G. Caporale' has been entrusted with the development of a system that provides different administrative levels with a spatial decisional and management tool to assist them in case of epidemics. A Web-based GIS has been designed to integrate epidemiological information with the corresponding geographic component. The system works on a disease basis (disease-specific maps) for fifteen of the most contagious diseases and is accessible through the Web, allowing for real-time recording of new outbreaks.