SO2 and copper tolerance exhibit an evolutionary trade-off in Saccharomyces cerevisiae.

Copper tolerance and SO2 tolerance are two well-studied phenotypic traits of Saccharomyces cerevisiae. The genetic bases of these traits are the allelic expansion at the CUP1 locus and reciprocal translocation at the SSU1 locus, respectively. Previous work identified a negative association between SO2 and copper tolerance in S. cerevisiae wine yeasts. Here we probe the relationship between SO2 and copper tolerance and show that an increase in CUP1 copy number does not always impart copper tolerance in S. cerevisiae wine yeast. Bulk-segregant QTL analysis was used to identify variance at SSU1 as a causative factor in copper sensitivity, which was verified by reciprocal hemizygosity analysis in a strain carrying 20 copies of CUP1. Transcriptional and proteomic analysis demonstrated that SSU1 over-expression did not suppress CUP1 transcription or constrain protein production and provided evidence that SSU1 over-expression induced sulfur limitation during exposure to copper. Finally, an SSU1 over-expressing strain exhibited increased sensitivity to moderately elevated copper concentrations in sulfur-limited medium, demonstrating that SSU1 over-expression burdens the sulfate assimilation pathway. Over-expression of MET 3/14/16, genes upstream of H2S production in the sulfate assimilation pathway increased the production of SO2 and H2S but did not improve copper sensitivity in an SSU1 over-expressing background. We conclude that copper and SO2 tolerance are conditional traits in S. cerevisiae and provide evidence of the metabolic basis for their mutual exclusivity. These findings suggest an evolutionary driver for the extreme amplification of CUP1 observed in some yeasts.

Universal modes: Calibration-free time-interleaved acquisition of modes.

PURPOSE: To introduce universal modes by applying the universal pulse concept to time-interleaved acquisition of modes (TIAMO), thereby achieving calibration-free B 1 + $$ {B}_1^{+} $$ inhomogeneity mitigation for body imaging at ultra-high fields. METHODS: Two databases of different RF arrays were used to demonstrate the feasibility of universal modes. The first comprised 31 cardiac in vivo data sets acquired at 7T while the second consisted of 6 simulated 10.5T pelvic data sets. Subject-specific solutions and universal modes were computed and subsequently evaluated alongside predefined default modes. For the cardiac database, subdivision into subpopulations was investigated. The optimization was performed using least-squares (LS) TIAMO and acquisition modes optimized for refocused echoes (AMORE). Finally, universal modes based on simulated pelvis data were applied in vivo at 10.5T. RESULTS: In all studied cases, the universal modes yield improvements over the predefined default modes of up to 51% (cardiac) and 30% (pelvic) in terms of median excitation error when using two modes. The subpopulation-specific cardiac solutions revealed a further improvement of universal modes at the expense of increased errors when applied outside the appropriate subpopulation. Direct application of simulation-based universal modes in vivo resulted in up to a 14% reduction in excitation error compared to default modes and up to a 34% reduction in peak 10 g local specific absorption rate (SAR) compared to subject-specific solutions. CONCLUSIONS: Universal modes are feasible for calibration-free B 1 + $$ {B}_1^{+} $$ inhomogeneity mitigation at ultra-high fields. In addition, simulation-based solutions can be applied directly in vivo, eliminating the need for large in vivo databases.

Improved 1 H body imaging at 10.5 T: Validation and VOP-enabled imaging in vivo with a 16-channel transceiver dipole array.

PURPOSE: To increase the RF coil performance and RF management for body imaging at 10.5 T by validating and evaluating a high-density 16-channel transceiver array, implementing virtual observation points (VOPs), and demonstrating specific absorption rate (SAR) constrained imaging in vivo. METHODS: The inaccuracy of the electromagnetic model of the array was quantified based on B1 + and SAR data. Inter-subject variability was estimated using a new approach based on the relative SAR deviation of different RF shims between human body models. The pTx performance of the 16-channel array was assessed in simulation by comparison to a previously demonstrated 10-channel array. In vivo imaging of the prostate was performed demonstrating SAR-constrained static RF shimming and acquisition modes optimized for refocused echoes (AMORE). RESULTS: The model inaccuracy of 29% and the inter-subject variability of 85% resulted in a total safety factor of 1.91 for pelvis studies. For renal and cardiac imaging, inter-subject variabilities of 121% and 141% lead to total safety factors of 2.25 and 2.45, respectively. The shorter wavelength at 10.5 T supported the increased element density of the 16-channel array which in turn outperformed the 10-channel version for all investigated metrics. Peak 10 g local SAR reduction of more than 25% without a loss of image quality was achieved in vivo, allowing a theoretical improvement in measurement efficiency of up to 66%. CONCLUSIONS: By validating and characterizing a 16-channel dipole transceiver array, this work demonstrates, for the first time, a VOP-enabled RF coil for human torso imaging enabling increased pTx performance at 10.5 T.

Toward accurate and fast velocity quantification with 3D ultrashort TE phase-contrast imaging.

PURPOSE: Traditional phase-contrast MRI is affected by displacement artifacts caused by non-synchronized spatial- and velocity-encoding time points. The resulting inaccurate velocity maps can affect the accuracy of derived hemodynamic parameters. This study proposes and characterizes a 3D radial phase-contrast UTE (PC-UTE) sequence to reduce displacement artifacts. Furthermore, it investigates the displacement of a standard Cartesian flow sequence by utilizing a displacement-free synchronized-single-point-imaging MR sequence (SYNC-SPI) that requires clinically prohibitively long acquisition times. METHODS: 3D flow data was acquired at 3T at three different constant flow rates and varying spatial resolutions in a stenotic aorta phantom using the proposed PC-UTE, a Cartesian flow sequence, and a SYNC-SPI sequence as reference. Expected displacement artifacts were calculated from gradient timing waveforms and compared to displacement values measured in the in vitro flow experiments. RESULTS: The PC-UTE sequence reduces displacement and intravoxel dephasing, leading to decreased geometric distortions and signal cancellations in magnitude images, and more spatially accurate velocity quantification compared to the Cartesian flow acquisitions; errors increase with velocity and higher spatial resolution. CONCLUSION: PC-UTE MRI can measure velocity vector fields with greater accuracy than Cartesian acquisitions (although pulsatile fields were not studied) and shorter scan times than SYNC-SPI. As such, this approach is superior to traditional Cartesian 3D and 4D flow MRI when spatial misrepresentations cannot be tolerated, for example, when computational fluid dynamics simulations are compared to or combined with in vitro or in vivo measurements, or regional parameters such as wall shear stress are of interest.

Arduino-Based Readout Electronics for Nuclear and Particle Physics.

Open Hardware-based microcontrollers, especially the Arduino platform, have become a comparably easy-to-use tool for rapid prototyping and implementing creative solutions. Such devices in combination with dedicated front-end electronics can offer low-cost alternatives for student projects, slow control and independently operating small-scale instrumentation. The capabilities can be extended to data taking and signal analysis at mid-level rates. Two detector realizations are presented, which cover the readouts of proportional counter tubes and of scintillators or wavelength-shifting fibers with silicon photomultipliers (SiPMs). The SiPMTrigger realizes a small-scale design for coincidence readout of SiPMs as a trigger or veto detector. It consists of a custom mixed signal front-end board featuring signal amplification, discrimination and a coincidence unit for rates of up to 200 kHz. The nCatcher transforms an Arduino Nano to a proportional counter readout with pulse shape analysis: time over threshold measurement and a 10-bit analog-to-digital converter for pulse heights. The device is suitable for low-to-medium-rate environments up to 5 kHz, where a good signal-to-noise ratio is crucial. We showcase the monitoring of thermal neutrons. For data taking and slow control, a logger board is presented that features an SD card and GSM/LoRa interface.

On-the-Fly Mass Spectrometry in Digital Microfluidics Enabled by a Microspray Hole: Toward Multidimensional Reaction Monitoring in Automated Synthesis Platforms.

We report an approach for the online coupling of digital microfluidics (DMF) with mass spectrometry (MS) using a chip-integrated microspray hole (µSH). The technique uses an adapted electrostatic spray ionization (ESTASI) method to spray a portion of a sample droplet through a microhole in the cover plate, allowing its chemical content to be analyzed by MS. This eliminates the need for chip disassembly or the introduction of capillary emitters for MS analysis, as required by state-of-the-art. For the first time, this allows the essential advantage of a DMF device─free droplet movement─to be retained during MS analysis. The broad applicability of the developed seamless coupling of DMF and mass spectrometry was successfully applied to the study of various on-chip organic syntheses as well as protein and peptide analysis. In the case of a Hantzsch synthesis, we were able to show that the method is very well suited for monitoring even rapid chemical reactions that are completed in a few seconds. In addition, the strength of the low resource consumption in such on-chip microsyntheses was demonstrated by the example of enzymatic brominations, for which only a minute amount of a special haloperoxidase is required in the droplet. The unique selling point of this approach is that the analyzed droplet remains completely movable after the MS measurement and is available for subsequent on-DMF chip processes. This is illustrated here for the example of MS analysis of the starting materials in the corresponding droplets before they are combined to investigate the reaction progress by DMF-MS further. This technology enables the ongoing and almost unlimited tracking of multistep chemical processes in a DMF chip and offers exciting prospects for transforming digital microfluidics into automated synthesis platforms.

Improved TSE imaging at ultrahigh field using nonlocalized efficiency RF shimming and acquisition modes optimized for refocused echoes (AMORE).

PURPOSE: To develop and evaluate a novel RF shimming optimization strategy tailored to improve the transmit efficiency in turbo spin echo imaging when performing time-interleaved acquisition of modes (TIAMO) at ultrahigh fields. THEORY AND METHODS: A nonlocalized efficiency shimming cost function is proposed and extended to perform TIAMO using acquisition modes optimized for refocused echoes (AMORE). The nonlocalized efficiency shimming was demonstrated in brain and knee imaging at 7 Tesla. Phantom and in vivo torso imaging studies were performed to compare the performance between AMORE and previously proposed TIAMO mode optimizations with and without localized constraints in turbo spin echo and gradient echo acquisitions. RESULTS: The proposed nonlocalized efficiency RF shimming produced a circularly polarized-like field with fewer signal dropouts in the brain and knee. For larger targets, AMORE was used and required a significantly lower transmitter voltage to produce a similar contrast to existing TIAMO mode design approaches for turbo spin echo as well as gradient echo acquisitions. In vivo, AMORE effectively reduced signal dropout in the interior torso while providing more uniform contrast with reduced transmit power. A local constraint further improved performance for a target region while maintaining performance in the larger FOV. CONCLUSION: AMORE based on the presented nonlocalized efficiency shimming cost function demonstrated improved contrast and SNR uniformity as well as increased transmit efficiency for both gradient echo and turbo spin echo acquisitions.

Performance and safety assessment of an integrated transmit array for body imaging at 7 T under consideration of specific absorption rate, tissue temperature, and thermal dose.

In this study, the performance of an integrated body-imaging array for 7 T with 32 radiofrequency (RF) channels under consideration of local specific absorption rate (SAR), tissue temperature, and thermal dose limits was evaluated and the imaging performance was compared with a clinical 3 T body coil. Thirty-two transmit elements were placed in three rings between the bore liner and RF shield of the gradient coil. Slice-selective RF pulse optimizations for B1 shimming and spokes were performed for differently oriented slices in the body under consideration of realistic constraints for power and local SAR. To improve the B1+ homogeneity, safety assessments based on temperature and thermal dose were performed to possibly allow for higher input power for the pulse optimization than permissible with SAR limits. The results showed that using two spokes, the 7 T array outperformed the 3 T birdcage in all the considered regions of interest. However, a significantly higher SAR or lower duty cycle at 7 T is necessary in some cases to achieve similar B1+ homogeneity as at 3 T. The homogeneity in up to 50 cm-long coronal slices can particularly benefit from the high RF shim performance provided by the 32 RF channels. The thermal dose approach increases the allowable input power and the corresponding local SAR, in one example up to 100 W/kg, without limiting the exposure time necessary for an MR examination. In conclusion, the integrated antenna array at 7 T enables a clinical workflow for body imaging and comparable imaging performance to a conventional 3 T clinical body coil.

The impact of 4D flow displacement artifacts on wall shear stress estimation.

PURPOSE: To investigate the amplitude and spatial distribution of errors in wall shear stress (WSS) values derived from 4D flow measurements caused by displacement artifacts intrinsic to the 4D flow acquisition. METHODS: Phase-contrast MRI velocimetry was performed in a model of a stenotic aorta using two different timing schemes, both of which are commonly applied in vivo but differ in their resulting displacement artifacts. Whereas one scheme is optimized to minimize the duration of the encoding gradients (herein called FAST), the other aims to specifically minimize displacement artifacts by synchronizing all three spatial-encoding time points (called ECHO). WSS estimates were calculated and compared to unbiased WSS values obtained by a 5-hour single-point imaging acquisition. In addition, MRI simulations based on computational fluid dynamics data were carried out to investigate the impact of gradient timings corresponding to different spatial resolutions. RESULTS: 4D flow displacement artifacts were found to have an impact on the quantified WSS peak values, spatial location, and overall WSS pattern. FAST leads to the underestimation of local WSS values in the phantom arch by up to 90%. Moreover, the corresponding WSS estimates depend on the image orientation. This effect was avoided using ECHO, which, however, results in biased WSS values within the stenosis, yielding an underestimation of peak WSS by up to 17%. Computational fluid dynamics-based simulation results show that the bias in WSS due to displacement artifacts increases with increasing spatial resolution, thus counteracting the resolution benefit for WSS due to reduced partial volume effects and segmentation errors. CONCLUSIONS: 4D flow displacement artifacts can significantly impact the WSS estimates and depend on the timing scheme as well as potentially the image orientation. Whereas FAST might allow correct WSS estimation for lower resolutions, ECHO is recommended especially when spatial resolutions of 1 mm and smaller are used. Users need to be aware of this nonnegligible effect, particularly when conducting inter-site studies or studies between vendors. The timing scheme should thus be explicitly mentioned in publications.

Phase-contrast acceleration mapping with synchronized encoding.

PURPOSE: To develop a phase-contrast (PC) -based method for direct and unbiased quantification of the acceleration vector field by synchronization of the spatial and acceleration encoding time points. The proposed method explicitly aims at in-vitro applications, requiring high measurement accuracy, as well as the validation of clinically relevant acceleration-encoded sequences. METHODS: A velocity-encoded sequence with synchronized encoding (SYNC SPI) was modified to allow direct acceleration mapping by replacing the bipolar encoding gradients with tripolar gradient waveforms. The proposed method was validated in two in-vitro flow cases: a rotation and a stenosis phantom. The thereby obtained velocity and acceleration vector fields were quantitatively compared to those acquired with conventional PC methods, as well as to theoretical data. RESULTS: The rotation phantom study revealed a systematic bias of the conventional PC acceleration mapping method that resulted in an average pixel-wise relative angle between the measured and theoretical vector field of (7.8 ± 3.2)°, which was reduced to (-0.4 ± 2.7)° for the proposed SYNC SPI method. Furthermore, flow features in the stenosis phantom were displaced by up to 10 mm in the conventional PC data compared with the acceleration-encoded SYNC SPI data. CONCLUSIONS: This work successfully demonstrates a highly accurate method for direct acceleration mapping. It thus complements the existing velocity-encoded SYNC SPI method to enable the direct and unbiased quantification of both the velocity and acceleration vector field for in vitro studies. Hence, this method can be used for the validation of conventional acceleration-encoded PC methods applicable in-vivo.

3D Free-breathing multichannel absolute B1+ Mapping in the human body at 7T.

PURPOSE: To introduce and investigate a method for free-breathing three-dimensional (3D) B1+ mapping of the human body at ultrahigh field (UHF), which can be used to generate homogenous flip angle (FA) distributions in the human body at UHF. METHODS: A 3D relative B1+ mapping sequence with a radial phase-encoding (RPE) k-space trajectory was developed and applied in 11 healthy subjects at 7T. An RPE-based actual flip angle mapping method was applied with a dedicated B1+ shim setting to calibrate the relative B1+ maps yielding absolute B1+ maps of the individual transmit channels. The method was evaluated in a motion phantom and by multidimensional in vivo measurements. Additionally, 3D gradient echo scans with and without static phase-only B1+ shims were used to qualitatively validate B1+ shim predictions. RESULTS: The phantom validation revealed good agreement for B1+ maps between dynamic measurement and static reference acquisition. The proposed 3D method was successfully validated in vivo by comparing magnitude and phase distributions with a 2D Cartesian reference. 3D B1+ maps free from visible motion artifacts were successfully acquired for 11 subjects with body mass indexes ranging from 19 kg/m2 to 34 kg/m2 . 3D respiration-resolved absolute B1+ maps indicated FA differences between inhalation and exhalation up to 15% for one channel and up to 24% for combined channels for shallow breathing. CONCLUSION: The proposed method provides respiration-resolved absolute 3D B1+ maps of the human body at UHF, which enables the investigation and development of 3D B1+ shimming and parallel transmission methods to further enhance body imaging at UHF.

Population sequencing reveals clonal diversity and ancestral inbreeding in the grapevine cultivar Chardonnay.

Chardonnay is the basis of some of the world's most iconic wines and its success is underpinned by a historic program of clonal selection. There are numerous clones of Chardonnay available that exhibit differences in key viticultural and oenological traits that have arisen from the accumulation of somatic mutations during centuries of asexual propagation. However, the genetic variation that underlies these differences remains largely unknown. To address this knowledge gap, a high-quality, diploid-phased Chardonnay genome assembly was produced from single-molecule real time sequencing, and combined with re-sequencing data from 15 different Chardonnay clones. There were 1620 markers identified that distinguish the 15 clones. These markers were reliably used for clonal identification of independently sourced genomic material, as well as in identifying a potential genetic basis for some clonal phenotypic differences. The predicted parentage of the Chardonnay haplomes was elucidated by mapping sequence data from the predicted parents of Chardonnay (Gouais blanc and Pinot noir) against the Chardonnay reference genome. This enabled the detection of instances of heterosis, with differentially-expanded gene families being inherited from the parents of Chardonnay. Most surprisingly however, the patterns of nucleotide variation present in the Chardonnay genome indicate that Pinot noir and Gouais blanc share an extremely high degree of kinship that has resulted in the Chardonnay genome displaying characteristics that are indicative of inbreeding.

A Sensor Fault Detection Scheme as a Functional Safety Feature for DC-DC Converters.

DC-DC converters are widely used in a large number of power conversion applications. As in many other systems, they are designed to automatically prevent dangerous failures or control them when they arise; this is called functional safety. Therefore, random hardware failures such as sensor faults have to be detected and handled properly. This proper handling means achieving or maintaining a safe state according to ISO 26262. However, to achieve or maintain a safe state, a fault has to be detected first. Sensor faults within DC-DC converters are generally detected with hardware-redundant sensors, despite all their drawbacks. Within this article, this redundancy is addressed using observer-based techniques utilizing Extended Kalman Filters (EKFs). Moreover, the paper proposes a fault detection and isolation scheme to guarantee functional safety. For this, a cross-EKF structure is implemented to work in cross-parallel to the real sensors and to replace the sensors in case of a fault. This ensures the continuity of the service in case of sensor faults. This idea is based on the concept of the virtual sensor which replaces the sensor in case of fault. Moreover, the concept of the virtual sensor is broader. In fact, if a system is observable, the observer offers a better performance than the sensor. In this context, this paper gives a contribution in this area. The effectiveness of this approach is tested with measurements on a buck converter prototype.

Configurable Sensor Model Architecture for the Development of Automated Driving Systems.

Sensor models provide the required environmental perception information for the development and testing of automated driving systems in virtual vehicle environments. In this article, a configurable sensor model architecture is introduced. Based on methods of model-based systems engineering (MBSE) and functional decomposition, this approach supports a flexible and continuous way to use sensor models in automotive development. Modeled sensor effects, representing single-sensor properties, are combined to an overall sensor behavior. This improves reusability and enables adaptation to specific requirements of the development. Finally, a first practical application of the configurable sensor model architecture is demonstrated, using two exemplary sensor effects: the geometric field of view (FoV) and the object-dependent FoV.

Novel Methods to Manipulate Autolysis in Sparkling Wine: Effects on Yeast.

Sparkling wine made by the traditional method (Méthode Traditionelle) develops a distinct and desirable flavour and aroma profile attributed to proteolytic processes during prolonged ageing on lees. Microwave, ultrasound and addition of ß-glucanase enzymes were applied to accelerate the disruption of Saccharomyces cerevisiae, and added to the tirage solution for secondary fermentation in traditional sparkling winemaking. Scanning electron microscopy and flow cytometry analyses were used to observe and describe yeast whole-cell anatomy, and cell integrity and structure via propidium iodide (PI) permeability after 6-, 12- and 18-months post-tirage. Treatments applied produced features on lees that were distinct from that of the untreated control yeast. Whilst control yeast displayed budding cells (growth features) with smooth, cavitated and flat external cell appearances; microwave treated yeast cells exhibited modifications like 'doughnut' shapes immediately after treatment (time 0). Similar 'doughnut'-shaped and 'pitted/porous' cell features were observed on progressively older lees from the control. Flow cytometry was used to discriminate yeast populations; features consistent with cell disruption were observed in the microwave, ultrasound and enzyme treatments, as evidenced by up to 4-fold increase in PI signal in the microwave treatment. Forward and side scatter signals reflected changes in size and structure of yeast cells, in all treatments applied. When flow cytometry was interpreted alongside the scanning electron microscopy images, bimodal populations of yeast cells with low and high PI intensities were revealed and distinctive 'doughnut'-shaped cell features observed in association with the microwave treatment only at tirage, that were not observed until 12 months wine ageing in older lees from the control. This work offers both a rapid approach to visualise alterations to yeast cell surfaces and a better understanding of the mechanisms of yeast lysis. Microwave, ultrasound or ß-glucanase enzymes are tools that could potentially initiate the release of yeast cell compounds into wine. Further investigation into the impact of such treatments on the flavour and aroma profiles of the wines through sensory evaluation is warranted.

Aromatic Higher Alcohols in Wine: Implication on Aroma and Palate Attributes during Chardonnay Aging.

The higher alcohols 2-phenylethanol, tryptophol, and tyrosol are a group of yeast-derived compounds that have been shown to affect the aroma and flavour of fermented beverages. Five variants of the industrial wine strain AWRI796, previously isolated due to their elevated production of the 'rose-like aroma' compound 2-phenylethanol, were characterised during pilot-scale fermentation of a Chardonnay juice. We show that these variants not only increase the concentration of 2-phenylethanol but also modulate the formation of the higher alcohols tryptophol, tyrosol, and methionol, as well as other volatile sulfur compounds derived from methionine, highlighting the connections between yeast nitrogen and sulfur metabolism during fermentation. We also investigate the development of these compounds during wine storage, focusing on the sulfonation of tryptophol. Finally, the sensory properties of wines produced using these strains were quantified at two time points, unravelling differences produced by biologically modulating higher alcohols and the dynamic changes in wine flavour over aging.

Development of a rotation phantom for phase contrast MRI sequence validation and quality control.

PURPOSE: To develop a reliable, consistent, and reproducible reference phantom for error quantification of phase-contrast MRI so it can be used for validation and quality control. METHODS: An air-driven rotation phantom consisting of a steadily rotating cylinder surrounded by a static ring both filled with agarose gel was developed. Rotational speed was measured and controlled in real time using an optical counter and a closed-loop controller. Consistency of the phantom was assessed by recording variations in rotational speed. The phantom was imaged with 2D phase-contrast MRI, and the velocity at each point was compared with analytically predicted velocity. Additionally, to examine reproducibility, the phantom was run with the same rotational speed on 2 different days and imaged using the same phase-contrast MRI protocol. RESULTS: The rotation phantom provided consistent rotational speed with 2 revolutions per minute SD from the set value for 20 min. Comparison between predicted and measured velocities demonstrated excellent agreement (intraclass correlation coefficient of 0.99). The RMS error in velocity components were less than 1% of maximum value. The scan-rescan experiment showed that the phantom can reproduce the same velocity distributions (intraclass correlation coefficient of 0.99) using the same rotational speed and MRI settings. CONCLUSION: The developed rotation phantom provided well-defined and reproducible linear velocity distributions, which can be used for systematic and quantitative error analysis of phase-contrast MRI for a range of known velocities.

Comparative genome analysis proposes three new Aureobasidium species isolated from grape juice.

Aureobasidium pullulans is the most abundant and ubiquitous species within the genus and is also considered a core component of the grape juice microflora. So far, a small number of other Aureobasidium species have been reported, that in contrast to A. pullulans, appear far more constrained to specific habitats. It is unknown whether grape juice is a reservoir of novel Aureobasidium species, overlooked in the course of conventional morphological and meta-barcoding analyses. In this study, eight isolates from grape juice taxonomically classified as Aureobasidium through ITS sequencing were subjected to whole-genome phylogenetic, synteny and nucleotide identity analyses, which revealed three isolates to likely represent newly discovered Aureobasidium species. Analyses of ITS and metagenomic sequencing datasets show that these species can be present in grape juice samples from different locations and vintages. Functional annotation revealed the Aureobasidium isolates possess the genetic potential to support growth on the surface of plants and grapes. However, the loss of several genes associated with tolerance to diverse environmental stresses suggest a more constrained ecological range than A. pullulans.

Investigating the effects of Aureobasidium pullulans on grape juice composition and fermentation.

Aureobasidium pullulans has been observed as one of the most abundant species in freshly pressed grape juice. Despite this, little is known about the consequences for the wine-making process associated with the presence and proliferation of this fungus, including its interaction with other ferment-derived microorganisms and impact on the composition of the resulting wine. In this study, the physiology of abundant A. pullulans grape juice isolates was investigated through lab scale fermentation trials, demonstrating the ability of this species to survive in grape juice while producing polysaccharides, polymers of malic acid (poly ß-malic acid) and enzymes with pectinase, ß - glucosidase and tannase activity. A possible antagonistic effect against yeast through competition for metals including Fe and Zn was also observed. Overall, the data suggests this abundant species could have important implications for wine production and quality.

Flow MR fingerprinting.

PURPOSE: To investigate the feasibility to quantify blood velocities within the magnetic resonance fingerprinting framework, while providing relaxometric maps of static tissue. METHODS: Bipolar gradients are inserted into an SSFP-based MRF sequence to achieve velocity-dependent signal phases, allowing tri-directional time-resolved velocity component quantification. The accuracy of both relaxometric mapping and velocity quantification was validated in vivo and in phantom studies. RESULTS: Simulations determined that even for strong cardiac cycle length variations (700-1400 ms) Flow-MRF determines accurate velocity maps deviating <0.1% from the ground truth on average. The cardiac cycle length variability only results in reduced velocity-to-noise ratios. Good agreement in the velocity quantification between a standard phase-contrast cine and the Flow-MRF sequence was reached in phantom experiments. Relaxometric phantom experiments determined mean deviations between Flow-MRF and spin-echo-based reference measurements of 89 ± 25 ms / 0.8 ± 2.5 ms over the range of 630-2630 ms / 49-145 ms for T1 / T2 , respectively. The in vivo study of a human knee determined mean T1 / T2 values of 1383 ± 75 ms / 26 ± 4 ms for the gastrocnemius muscle that agree with literature values. CONCLUSION: Flow-MRF presents a novel way of quantifying velocities while simultaneously providing relaxometric maps of static tissue and it can potentially be a viable method to accelerate the inherently long acquisition times of time-resolved velocity quantification.