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1.
Z Rheumatol ; 77(2): 113-126, 2018 Mar.
Artigo em Alemão | MEDLINE | ID: mdl-28929232

RESUMO

BACKGROUND: Rheumatoid arthritis (RA) has an increased number of comorbidities compared with the general population. OBJECTIVE: Study aim was to collect epidemiological data on prevalence, incidence and comorbidities of RA as well as utilization of outpatient and inpatient care services. MATERIAL AND METHODS: In an age and gender-adjusted case control study, a total of 3.4 million patients insured by the AOK Baden-Württemberg were analysed with respect to visits to physicians, prevalence, incidence and comorbidities of RA. The study was based on out- and inpatient diagnoses from 2013. RESULTS: The RA prevalence was 0.64% (n = 26,919), the incidence was 0.04%. Patients with RA have significant more comorbidities in almost all diagnosis groups, especially in musculoskeletal and cardiovascular diseases, compared to a control group (n = 181,209). 22.8% of RA patients had not contacted an internist rheumatologist, orthopedist or orthopedic surgeon. Biological disease-modifying anti-rheumatic drugs (DMARDs) were almost exclusively prescribed by internist rheumatologists, while conventional DMARDs were equally prescribed by general practitioners and rheumatologists. Of the RA patients 32.6% were hospitalized at least once a year and were nearly twice as frequently inpatient as the control group. CONCLUSION: RA patients need more in- and outpatient healthcare services and suffer significantly more often from comorbidities. The general practitioner is the most frequently visited physician. Other consulted physicians are rheumatologists, ophthalmologists, orthopedists/orthopedic surgeons and internists not specialized in rheumatology. The study highlights the need to create consensus treatment algorithms and maintain a close interdisciplinary and intersectoral cooperation and communication.


Assuntos
Artrite Reumatoide , Pacientes Ambulatoriais , Artrite Reumatoide/tratamento farmacológico , Artrite Reumatoide/epidemiologia , Estudos de Casos e Controles , Comorbidade , Alemanha/epidemiologia , Humanos , Pacientes Internados , Prevalência
2.
Orthopade ; 45(12): 1015-1026, 2016 Dec.
Artigo em Alemão | MEDLINE | ID: mdl-27518117

RESUMO

BACKGROUND: Musculoskeletal illnesses and injuries are among the most common ailments in the Federal Republic of Germany. In 2008 they generated costs of nearly 29 billion euros. Figures about their incidence and prevalence are necessary for a demand-oriented planning of future patient-centred care. METHOD: Pseudonymised data of 3.8 million people insured by AOK Baden-Württemberg between 2008 and 2013 were evaluated. The diagnoses were assigned to nine injury groups. For outpatient care confirmed diagnoses were considered, and for inpatient care both primary and secondary diagnoses were considered. For all patients with structural knee injuries, it was evaluated whether they made use of one of five eligible treatment paradigms either in the quarter in which they were injured or in the following quarter. RESULTS: 418,257 patients were treated in 2013 for at least one new-onset injury (10.9 % of all insurees); 86,783 insurees (2.3 % of all insurees) had a newly occurring knee injury. The vast majority of the patients were treated by specialist doctors. While magnetic resonance imaging clearly increased during the observation period, the incidence of surgical therapy did not change. Striking are the different age distributions regarding the types of injuries, with a high injury incidence amongst young men and a significant increase in injuries between 2008 and 2013, especially amongst women. CONCLUSION: For the first time, the data quantify the knee injury incidences of a large cohort in Germany. They show which inpatient and outpatient health care services have been claimed and that an age- and gender-adapted prevention and an increased awareness are needed.


Assuntos
Assistência Ambulatorial/estatística & dados numéricos , Hospitalização/estatística & dados numéricos , Pacientes Internados/estatística & dados numéricos , Reembolso de Seguro de Saúde/estatística & dados numéricos , Traumatismos do Joelho/epidemiologia , Traumatismos do Joelho/terapia , Adolescente , Adulto , Distribuição por Idade , Idoso , Idoso de 80 Anos ou mais , Criança , Pré-Escolar , Feminino , Alemanha/epidemiologia , Humanos , Incidência , Lactente , Recém-Nascido , Traumatismos do Joelho/diagnóstico , Masculino , Pessoa de Meia-Idade , Fatores de Risco , Distribuição por Sexo , Adulto Jovem
3.
Phys Chem Chem Phys ; 15(20): 7777-84, 2013 May 28.
Artigo em Inglês | MEDLINE | ID: mdl-23598350

RESUMO

Neutron reflectometry is used to study in situ the intercalation of lithium into amorphous silicon electrodes. The experiments are done using a closed three-electrode electrochemical cell setup. As a working electrode, an about 40 nm thick amorphous silicon layer is used that is deposited on a 1 cm thick quartz substrate coated with palladium as a current collector. The counter electrode and the reference electrode are made of lithium metal. Propylene carbonate with 1 M LiClO4 is used as an electrolyte. The utility of the cell is demonstrated during neutron reflectometry measurements where Li is intercalated at a constant current of 100 µA (7.8 µA cm(-2)) for different time steps. The results show (a) that the change in Li content in amorphous silicon and the corresponding volume expansion can be monitored, (b) that the formation of the solid electrolyte interphase becomes visible and (c) that an irreversible capacity loss is present.


Assuntos
Fontes de Energia Elétrica , Lítio/química , Silício/química , Técnicas Eletroquímicas , Eletrodos , Íons/química , Difração de Nêutrons
4.
Phys Rev Lett ; 104(23): 231801, 2010 Jun 11.
Artigo em Inglês | MEDLINE | ID: mdl-20867227

RESUMO

First observations of the B(s)(0) → D(s)(*-) π+, B(s)(0) → D(s)(-) ρ+ and B(s)(0) → D(s)(*-) ρ+ decays are reported together with measurements of their branching fractions: B(B(s)(0) → D(s)(*-) π+) = [2.4(-0.4)(+0.5)(stat) ± 0.3(syst) ± 0.4(f(s))]×10(-3), B(B(s)(0) → D(s)(-) ρ+) = [8.5(-1.2)(+1.3)(stat) ± 1.1(syst) ± 1.3(f(s))]×10(-3) and B(B(s)(0) → D(s)(*-) ρ+) = [11.9(-2.0)(+2.2)(stat) ± 1.7(syst) ± 1.8(f(s))]×10(-3) (f(s) = N(B(s)(*) B(s)(*))/N(bb)). From helicity-angle distributions, we measured the longitudinal polarization fraction in B(s)(0) → D(s)(*-) ρ+ decays to be f(L)(B(s)(0) → D(s)(*-) ρ+) = 1.05(-0.10)(+0.08)(stat)(-0.04)(+0.03)(syst). These results are based on a 23.6 fb(-1) data sample collected at the Υ(5S) resonance with the Belle detector at the KEKB e+ e- collider.


Assuntos
Partículas Elementares , Teoria Quântica
5.
Eur Phys J C Part Fields ; 79(7): 622, 2019.
Artigo em Inglês | MEDLINE | ID: mdl-31402844

RESUMO

A method is proposed to measure the photon polarisation parameter λ γ in b → s γ transitions using an amplitude analysis of B → K π π γ decays. Simplified models of the K π π system are used to simulate B + → K + π - π + γ and B 0 → K + π - π 0 γ decays, validate the amplitude analysis method, and demonstrate the feasibility of a measurement of the λ γ parameter irrespective of the model parameters. Similar sensitivities to λ γ are obtained with both the charged and neutral hadronic systems. In the absence of any background and distortion due to experimental effects, the statistical uncertainty expected from an analysis of B + → K + π - π + γ decays in an LHCb data set corresponding to an integrated luminosity of 9  fb - 1 is estimated to be 0.009. A similar measurement using B 0 → K + π - π 0 γ decays in a Belle II data sample corresponding to an integrated luminosity of 5  ab - 1 would lead to a statistical uncertainty of 0.018.

6.
Cell Signal ; 28(6): 620-30, 2016 Jun.
Artigo em Inglês | MEDLINE | ID: mdl-26976208

RESUMO

Bone marrow mesenchymal stem cells' (BM-MSCs) role in multiple myeloma (MM) pathogenesis is recognized. Recently, we have published that co-culture of MM cell lines with BM-MSCs results in mutual modulation of phenotype and proteome (via translation initiation (TI) factors eIF4E/eIF4GI) and that there are differences between normal donor BM-MSCs (ND-MSCs) and MM BM-MSCs (MM-MSCs) in this crosstalk. Here, we aimed to assess the involvement of soluble BM-MSCs' (ND, MM) components, more easily targeted, in manipulation of MM cell lines phenotype and TI with specific focus on microvesicles (MVs) capable of transferring critical biological material. We applied ND and MM-MSCs 72h secretomes to MM cell lines (U266 and ARP-1) for 12-72h and then assayed the cells' (viability, cell count, cell death, proliferation, cell cycle, autophagy) and TI (factors: eIF4E, teIF4GI; regulators: mTOR, MNK1/2, 4EBP; targets: cyclin D1, NFκB, SMAD5, cMyc, HIF1α). Furthermore, we dissected the secretome into >100kDa and <100kDa fractions and repeated the experiments. Finally, MVs were isolated from the ND and MM-MSCs secretomes and applied to MM cell lines. Phenotype and TI were assessed. Secretomes of BM-MSCs (ND, MM) significantly stimulated MM cell lines' TI, autophagy and proliferation. The dissected secretome yielded different effects on MM cell lines phenotype and TI according to fraction (>100kDa- repressed; <100kDa- stimulated) but with no association to source (ND, MM). Finally, in analyses of MVs extracted from BM-MSCs (ND, MM) we witnessed differences in accordance with source: ND-MSCs MVs inhibited proliferation, autophagy and TI whereas MM-MSCs MVs stimulated them. These observations highlight the very complex communication between MM and BM-MSCs and underscore its significance to major processes in the malignant cells. Studies into the influential MVs cargo are underway and expected to uncover targetable signals in the regulation of the TI/proliferation/autophagy cascade.


Assuntos
Células-Tronco Mesenquimais/metabolismo , Mieloma Múltiplo/genética , Iniciação Traducional da Cadeia Peptídica , Autofagia , Linhagem Celular Tumoral , Proliferação de Células , Células Cultivadas , Fator de Iniciação 4E em Eucariotos/metabolismo , Fator de Iniciação Eucariótico 4G/metabolismo , Humanos , Mieloma Múltiplo/metabolismo , Mieloma Múltiplo/patologia
7.
Dev Comp Immunol ; 29(4): 305-14, 2005.
Artigo em Inglês | MEDLINE | ID: mdl-15859235

RESUMO

Snail hemocytes mobilise ROS-generating enzymes during oxidative burst similar to those of mammalian leukocytes. We report herein the identification of an inducible Cu/Zn superoxide dismutase, which converts O2- to H2O2, in hemocytes of the pond snail Lymnaea stagnalis. The deduced amino acid sequence with all characteristic residues (His44,46,61,69,78 and 118, Asp81, Cys55/144, Arg141 and the Greek Key loop region Glu119-Leu/Val142) includes an open reading frame of 155 AA. Changes in Cu/ZnSOD gene expression induced by stimulation with Zymosan or trematode larvae were examined in a time course. Activated hemocytes significantly up-regulate Cu/ZnSOD expression during 2-48 h upon stimulation with the maximal induction at 45 min during phagocytosis and at 12 h during encapsulations. This increase in Cu/ZnSOD expression paralleled the increasing production of hydrogen peroxide by hemocytes. Thus, intracellular or extracellular targets elicit an induced expression of Cu/ZnSOD and the generation of elevated amounts of hydrogen peroxide by L. stagnalis hemocytes, reflecting a significant activation of their host defense function.


Assuntos
Hemócitos/enzimologia , Peróxido de Hidrogênio/metabolismo , Lymnaea/enzimologia , Superóxido Dismutase/metabolismo , Sequência de Aminoácidos , Animais , Sequência de Bases , Eletroforese em Gel de Poliacrilamida , Hemócitos/metabolismo , Immunoblotting , Lymnaea/genética , Lymnaea/metabolismo , Lymnaea/parasitologia , Dados de Sequência Molecular , Fagocitose/fisiologia , Reação em Cadeia da Polimerase Via Transcriptase Reversa , Schistosoma mansoni , Esquistossomose mansoni/enzimologia , Esquistossomose mansoni/metabolismo , Superóxido Dismutase/genética , Zimosan/metabolismo
8.
J Phys Chem B ; 109(15): 7159-68, 2005 Apr 21.
Artigo em Inglês | MEDLINE | ID: mdl-16851816

RESUMO

The direct electropolymerization of benzene dissolved in the ionic liquid 1-hexyl-3-methylimidazolium tris(pentafluoroethyl)trifluorophosphate was studied at room temperature applying the electrochemical quartz-crystal microbalance technique. Analysis of the damping changes showed that the Sauerbrey equation could be applied for data evaluation. In the polymer, every third to fourth benzene ring carried a positive charge in the oxidized state. During electropolymerization, some ionic liquid was absorbed in the growing polymer. The redox behavior was characterized by wide peaks typical for conducting polymers. Charge neutrality of the polymer during redox cycling was maintained by anion and cation exchange with the ionic liquid. With increasing scan rate, cation exchange became more and more important.


Assuntos
Benzeno/química , Físico-Química/métodos , Eletroquímica/métodos , Troca Iônica , Polímeros/química , Cátions , Íons , Microscopia Eletrônica de Varredura , Modelos Químicos , Oxirredução , Oxigênio/química , Espectrofotometria , Temperatura , Termodinâmica
9.
Endocrinology ; 137(11): 4925-34, 1996 Nov.
Artigo em Inglês | MEDLINE | ID: mdl-8895365

RESUMO

Follistatin (FS) is the specific binding protein of activin; it has a broad tissue distribution and is also found in serum. The ovary has the highest level of FS expression, but ovariectomy does not cause a permanent reduction in the serum FS level. Therefore, the source of FS in serum is still elusive. As a regulatable, nongonadal source of serum FS could influence ovarian and pituitary-derived hormone secretion and thus reproductive function, we searched for a source of extragonadal FS expression that might contribute to the FS protein level in serum. We found that endothelial cells from blood vessels express FS messenger RNA (mRNA) and protein; therefore, we studied the regulation of steady state levels of FS mRNA in porcine endothelial cells from aorta (AEC) and brain microvessels (BMVEC) in tissue culture. For detection of FS mRNA, a specific 32P-radiolabeled antisense probe and a S1-nuclease protection assay were used. FS steady state levels of AEC decreased with time in culture, i.e. postconfluent AEC had lower FS mRNA levels than confluent cultures, which, in turn, had lower FS mRNA levels than subconfluent cell cultures. FS mRNA levels in AEC were induced by increasing concentrations of FCS and stimulated by 30 micrograms/ml endothelial cell growth supplement. FS mRNA levels in AEC and BMVEC increased approximately 20-fold within 4 h during incubation of the cells with 100 nM phorbol 12-myristate, 13-acetate, whereas 0.5 nmol/ml forskolin tested in AEC for between 4-48 h had no significant effect. Furthermore, 0.1 microM ocadaic acid, an inhibitor of serine/threonine phosphatases 1 and 2A, caused a significant increase in FS mRNA levels. FS mRNA levels in AEC were not significantly affected by various concentrations of porcine FSH, epidermal growth factor, or retinoic acid for between 4-48 h. Treatment of the cells with 0.01-10 micrograms/ml bacterial lipopolysaccharides (LPS) caused a dose-dependent increase (up to 10-fold) in FS mRNA steady state level in AEC, whereas 1-1000 nM RU 28362, a synthetic glucocorticoid, inhibited FS mRNA steady state levels in a dose-dependent manner. The induction of FS mRNA with 1 microgram/ml LPS was completely blocked by 100 nM RU 28362, and the stimulatory effects of LPS were only visible after 4 h of treatment, not after 24 or 48 h. The same effects were observed with BMVEC. We, furthermore, analyzed FS protein secretion of AEC by Western blotting and demonstrated that FS proteins were secreted into the culture medium upon stimulation with LPS. None of these treatments had an obvious effect on the ratio of the two different forms of FS mRNA (FS 344:FS 317). Besides the expression of FS mRNA in AEC and BMVEC, FS mRNA is also expressed in uncultured plexus choroideus epithel and meninges, and FS protein is found in human cerebrospinal fluid. From this study it is concluded that 1) endothelial cells from different tissues produce FS mRNA; 2) the FS mRNA levels of AEC and BMVEC are subjected to regulation by FCS, endothelial cell growth supplement, bacterial LPS, and the glucocorticoid RU 28362; 3) phosphatases and the protein kinase C-dependent, but not the protein kinase A-dependent, pathway are involved in regulating the steady state levels of FS mRNA in AEC and BMVEC; and 4) endothelial cells produce and secrete FS protein and are thus a likely source of FS in serum.


Assuntos
Androstanóis/farmacologia , Endotélio Vascular/metabolismo , Glucocorticoides/farmacologia , Glicoproteínas/biossíntese , Lipopolissacarídeos/farmacologia , Transcrição Gênica/efeitos dos fármacos , Animais , Aorta , Encéfalo , Divisão Celular/efeitos dos fármacos , Células Cultivadas , Plexo Corióideo/metabolismo , Endotélio Vascular/citologia , Endotélio Vascular/efeitos dos fármacos , Feminino , Folistatina , Humanos , Inibinas/biossíntese , Cinética , Meninges/metabolismo , Microcirculação , Especificidade de Órgãos , Ovariectomia , Ovário/metabolismo , RNA Mensageiro/biossíntese , Salmonella , Suínos , Acetato de Tetradecanoilforbol/farmacologia
10.
Neurology ; 53(7): 1584-7, 1999 Oct 22.
Artigo em Inglês | MEDLINE | ID: mdl-10534274

RESUMO

Lipoteichoic and teichoic acids are components of the cell wall of Streptococcus pneumoniae. A recently developed enzyme immunoassay was used in patients with pneumococcal meningitis to investigate lipoteichoic and teichoic acid concentrations in CSF at the first lumbar puncture in relation to the clinical outcome determined by the Glasgow Outcome Scale. Lipoteichoic and teichoic acid concentrations in CSF were significantly associated with neurologic sequelae and mortality in S. pneumoniae meningitis.


Assuntos
Lipopolissacarídeos/líquido cefalorraquidiano , Meningite Pneumocócica/líquido cefalorraquidiano , Meningite Pneumocócica/fisiopatologia , Ácidos Teicoicos/líquido cefalorraquidiano , Adolescente , Adulto , Idoso , Idoso de 80 Anos ou mais , Pré-Escolar , Avaliação da Deficiência , Feminino , Escala de Coma de Glasgow , Humanos , Técnicas Imunoenzimáticas/métodos , Lactente , Recém-Nascido , Masculino , Meningite Pneumocócica/complicações , Meningite Pneumocócica/mortalidade , Pessoa de Meia-Idade , Doenças do Sistema Nervoso/etiologia , Concentração Osmolar , Punção Espinal
11.
Eur J Endocrinol ; 142(5): 537-44, 2000 May.
Artigo em Inglês | MEDLINE | ID: mdl-10802535

RESUMO

OBJECTIVE: The relation of activins (dimers of the beta-subunits of inhibin) and follistatin (FS) (their binding protein) affect the growth and differentiation of many cell types. Activin- and FS-mRNAs show a widespread co-expression throughout the organism, indicating an essential role for the FS/activin system in diverse physiological processes. The present study was performed to investigate FS-, activin betaA-, and activin beta B-mRNA expression in porcine tissues and to compare the relative mRNA tissue distribution by a newly developed multiplex S1 nuclease protection assay. METHODS: Twenty micrograms total RNA from different porcine tissues were subjected to multiplex S1 analysis. Specific mRNA expression was determined by measurements of optical densities on autoradiographs. RESULTS: Activin beta A-mRNA expression was abundant in the ovary, adrenal gland, fat, vein, artery and uterus, activin beta B-mRNA was highly expressed in the ovary, pituitary, uterus, placenta, aorta and cerebellum. FS-mRNA showed a widespread expression with high levels in ovary, uterus, cerebellum, placenta and fat. The comparison of relative activin beta A-, activin beta B- and FS-mRNA expression within a certain tissue showed a predominance of activin beta A-mRNA in the adrenal gland, fat, artery, spinal cord, cerebrum and colon and of activin beta B-m RNA in pituitary, testis and placenta, while FS-mRNA levels exceeded those of activin subunits in epididymis, liver, lymphoid tissue, muscle, intestine, cerebellum, ovary and uterus. CONCLUSIONS: The presented data provide an overview of FS-, activin beta A-, and activin beta B-mRNA steady state levels in porcine tissues.


Assuntos
Glicoproteínas/biossíntese , Substâncias de Crescimento/biossíntese , Inibinas/biossíntese , RNA Mensageiro/análise , Endonucleases Específicas para DNA e RNA de Cadeia Simples/análise , Ativinas , Animais , Folistatina , Glicoproteínas/genética , Substâncias de Crescimento/genética , Inibinas/genética , Reação em Cadeia da Polimerase/métodos , Sondas RNA , Suínos , Distribuição Tecidual , Transcrição Gênica , Leveduras
12.
Eur J Endocrinol ; 143(6): 809-16, 2000 Dec.
Artigo em Inglês | MEDLINE | ID: mdl-11124865

RESUMO

OBJECTIVE: Follistatin (FS) is the specific binding protein of activin and expression of both factors is regulated by inflammatory agents. Therefore, FS concentrations were determined in cerebrospinal fluid (CSF) of patients with bacterial and viral meningitis or multiple sclerosis (MS), as well as in the CSF of patients without meningial inflammation or autoimmune diseases. Furthermore, a mouse pneumococcal meningitis model was used to localise the cellular sources of FS in brains of normal and meningitic mice. METHODS: FS concentrations in CSF were determined by ELISA; FS in mice was localised by in situ hybridisation and immunohistochemistry. RESULTS: FS concentrations were > or =0.4 microg/l in 22 of 66 CSF samples of meningitis patients versus 2 of 27 CSF samples from patients with multiple sclerosis (P<0.05) and 2 of 41 CSF specimen from patients without neuroinflammatory diseases (P<0.01). In the CSF of patients with meningitis, the concentration of FS was correlated with total protein (P<0.005) and lactate concentrations (P<0.05), but not with leukocyte counts, interval between onset of disease and CSF analysis, or clinical outcome. The CSF-to-serum ratios of FS and albumin also correlated significantly (P<0.0005). In some patients with meningitis the CSF-to-serum ratios suggested that the elevated FS in CSF did not originate from serum alone. FS was localised in mice brains to neurones of the hippocampus, dentate gyrus, neocortex, and to the choroid plexus. Analyses of brains and other organs from uninfected and infected animals sacrificed 6-36 h after infection did not reveal any obvious differences in the distribution and intensity of FS mRNA and protein expression. CONCLUSIONS: The concentration of FS in humans is elevated during meningitis. In some patients the increase is caused by a release of FS from brain into CSF. Data from the mouse meningitis model suggest that increased CSF concentrations of FS in meningitis appear not to be accompanied by an elevated number of cells containing FS mRNA or protein in the brain.


Assuntos
Glicoproteínas/líquido cefalorraquidiano , Glicoproteínas/genética , Meningites Bacterianas/líquido cefalorraquidiano , Meningite Viral/líquido cefalorraquidiano , Animais , Encéfalo/metabolismo , Encéfalo/patologia , Modelos Animais de Doenças , Ensaio de Imunoadsorção Enzimática , Feminino , Folistatina , Regulação da Expressão Gênica , Glicoproteínas/sangue , Substâncias de Crescimento/sangue , Substâncias de Crescimento/líquido cefalorraquidiano , Substâncias de Crescimento/genética , Humanos , Masculino , Meningites Bacterianas/sangue , Meningite Pneumocócica/sangue , Meningite Pneumocócica/líquido cefalorraquidiano , Meningite Viral/sangue , Camundongos , Camundongos Endogâmicos C57BL , Esclerose Múltipla/sangue , Esclerose Múltipla/líquido cefalorraquidiano , Valores de Referência , Streptococcus pneumoniae
13.
Neurosurgery ; 38(5): 926-33, 1996 May.
Artigo em Inglês | MEDLINE | ID: mdl-8727817

RESUMO

We report 43 consecutive surgically treated patients with pyogenic (37 patients) and tuberculous (6 patients) osteomyelitis of the thoracic and lumbar spine encountered within an 8-year period, including 1 with late recurrence after 15 months. There were 24 men and 18 women, ranging in age from 21 to 83 years. Twenty-six patients were in poor general condition because of associated illnesses, especially diabetes mellitus. Disease occurred at the thoracic level in 19 patients and on the lumbar spine in 24. After diagnosis, five patients were merely treated by posterior decompression; three of them, however, required further surgery for recurrent infection, spinal instability, and secondary neurological impairment. They are included in the 40 patients who underwent combined posterior débridement and internal fixation with transpedicular screw-rod systems. Autologous interbody bone grafting was performed simultaneously in 18 patients and in a second stage operation in 21 patients. One of them (tuberculous) experienced early recurrence and required anterior fusion. In two patients, methylmethacrylate packing was used for spine reconstruction; one of them had a late recurrence. Of the 26 patients with preoperative marked or severe neurological deficit (Frankel Grades A, 2 patients; B, 1 patient; C, 17 patients; and D, 6 patients), 23 (88%) had significant improvement of one grade (15 patients) or more (8 patients). There were no permanent complications. However, intensive care treatment was necessary in 20 of the 26 patients in reduced general condition (mean age, 72 yr). Two patients required further surgery because of postoperative epidural hematoma and pedicle screw malpositioning. In conclusion, most patients with thoracic and lumbar osteomyelitis can be successfully treated by combined débridement and internal fixation using only a posterior approach. Autogenous interbody bone grafting can be simultaneously performed and allows early mobilization of the patient.


Assuntos
Discite/cirurgia , Vértebras Lombares/cirurgia , Osteomielite/cirurgia , Vértebras Torácicas/cirurgia , Tuberculose da Coluna Vertebral/cirurgia , Adulto , Idoso , Idoso de 80 Anos ou mais , Parafusos Ósseos , Transplante Ósseo , Discite/diagnóstico por imagem , Deambulação Precoce , Feminino , Seguimentos , Humanos , Vértebras Lombares/diagnóstico por imagem , Masculino , Pessoa de Meia-Idade , Mielografia , Osteomielite/diagnóstico por imagem , Complicações Pós-Operatórias/diagnóstico por imagem , Complicações Pós-Operatórias/cirurgia , Recidiva , Reoperação , Fusão Vertebral/instrumentação , Vértebras Torácicas/diagnóstico por imagem , Tomografia Computadorizada por Raios X , Resultado do Tratamento , Tuberculose da Coluna Vertebral/diagnóstico por imagem
14.
Arch Dermatol Res ; 262(3): 285-99, 1978 Aug 28.
Artigo em Alemão | MEDLINE | ID: mdl-568919

RESUMO

Thin slices of s.c. implanted B-16 melanomas as well as of human melanomas have been incubated for 5 h with (H3) Uridine and (H3) Thymidine in the presence of different chemotherapeutical agents, whose concentration was equivalent to the tenfold therapeutical daily dose in men. In this short term test model, the sensitivity of a melanoma to a chemotherapeutical agent is indicated by the inhibition of the nucleoside uptake by more than 50%. The in vitro sensitivity rates, each based on 10--30 melanomas, are compared to the in vivo sensitivity rates. Sensitivity is indicated by the increase of life span (greater than 25%) in the melanoma bearing mice respectively by the regression of human melanoma metastases (greater than 50%). -- The in vivo sensitivity of the B-16 melanomas, evaluated by the uridine and/or thymidine uptake, was in line with the in vivo sensitivity to all chemotherapeutical agents with the exeption of Adriamycine and DTIC. The in vitro sensitivity of human melanomas to Dactinomicine, Vincristine, BCNU and DTIC corresponds to the in vivo sensitivity whereas no in vitro/in vivo correspondence could be observed in testing Bleomycine, Procarbacine and 5-Fluorouracile. Comparing the sensitivity of B-16 and human melanomas, similarity was observed in vitro but not in vivo.


Assuntos
Antineoplásicos/uso terapêutico , Melanoma/tratamento farmacológico , Neoplasias Experimentais/tratamento farmacológico , Neoplasias Cutâneas/tratamento farmacológico , Animais , Cricetinae , Resistência a Medicamentos , Humanos , Técnicas In Vitro , Melanoma/metabolismo , Camundongos , Nucleosídeos/metabolismo , Timidina/metabolismo , Uridina/metabolismo
15.
Magn Reson Imaging ; 9(1): 67-72, 1991.
Artigo em Inglês | MEDLINE | ID: mdl-2056853

RESUMO

A flow model was used to study the appearance of poststenotic jets in MRI. Jets in CuSO4-doped water and bovine blood were imaged by spin-echo (SE) and fast-field-echo (FFE) pulse sequences at different degrees of stenosis and various flow rates. On flow-compensated FFE images, the jets were characterized by signal void if the mean flow velocity within the stenosis exceeded a limit, which was independent of the degree of the stenosis and the type of the fluid. On SE images and on FFE images without flow compensation, signal void occurred at significantly lower flow velocity. The extension of the poststenotic signal void on flow-compensated FFE images was increased by either reduction of the pixel diameter or by prolongation of the echo time. However, it was independent of the orientation of the imaging plane relative to the direction of flow. The results have an impact on attempts to use signal void for the assessment of turbulent jets with MRI.


Assuntos
Imageamento por Ressonância Magnética , Doenças Vasculares/fisiopatologia , Animais , Sangue , Velocidade do Fluxo Sanguíneo/fisiologia , Bovinos , Constrição Patológica/patologia , Constrição Patológica/fisiopatologia , Cobre , Sulfato de Cobre , Análise de Fourier , Aumento da Imagem/métodos , Imageamento por Ressonância Magnética/métodos , Modelos Cardiovasculares , Reologia , Doenças Vasculares/patologia , Água
16.
Mutat Res ; 169(3): 159-70, 1986 Mar.
Artigo em Inglês | MEDLINE | ID: mdl-3951468

RESUMO

The cellular uptake, the cytotoxicity and the induction of resistance to 6-thioguanine (6-TG) in Chinese hamster V79 cells exposed to insoluble crystalline trivalent chromium [Cr(III)], Cr2O3, were investigated. Intracytoplasmic Cr2O3 crystalline particle-containing vacuoles were observed by electron microscopy. Concentrations of 50-200 micrograms/ml did not have a marked killing effect but did show a predominantly concentration-dependent inhibitory effect on cell cycle progression with accumulation of cells in G2 phase. Exposure for 18 h to Cr2O3 induced a statistically significant (p less than 0.001) increase in the mutation frequency of up to 10-fold over the controls. Expression time was 6 days for the lowest concentration and 9 days for the highest. Culture of 6-TGr clones in selective media indicated that they were mutants at the hypoxanthine-guanine phosphoribosyl transferase (HGPRT) locus. Examination of growth patterns of Cr2O3-induced mutants showed that, after a delay in reinitiating cell growth, they had varying growth kinetics. The results indicate the ability of a particulate (Cr(III) compound to induce mutation in a mammalian cell system and the usefulness of such systems for detecting genotoxic insoluble metal compounds.


Assuntos
Compostos de Cromo , Cromo/farmacologia , Fibroblastos/efeitos dos fármacos , Animais , Ciclo Celular/efeitos dos fármacos , Divisão Celular/efeitos dos fármacos , Linhagem Celular , Cromo/metabolismo , Cricetinae , Cricetulus , Citoplasma/ultraestrutura , Fibroblastos/metabolismo , Fibroblastos/ultraestrutura , Pulmão , Masculino , Testes de Mutagenicidade , Fagocitose , Solubilidade
17.
Hum Exp Toxicol ; 18(5): 322-6, 1999 May.
Artigo em Inglês | MEDLINE | ID: mdl-10372754

RESUMO

1. Chemical risk assessment integrates the identification of hazards and the human exposure levels which can be established from external and/or internal exposure data. 2. The availability of biomonitoring and metabolism animal data, the skin penetration ability, and the existence of atmospheric threshold limit values were examined for twelve substances of the European first list of priority existing substances. This investigation was focused on workplace exposures and on urinary biomarkers of exposure. Appropriate biomonitoring data appeared to be available for two substances: styrene and trichloroethylene. Some biomonitoring research has been conducted on acrylonitrile, buta-1,3-diene, cyclohexane, 1,4-dichlorobenzene, hydrogen fluoride, 2-(2-methoxyethoxy)ethanol, however additional studies could be usefully carried out. No biomonitoring data are available for alkanes, C10-13, chloro; benzene, C10-13-alkyl derivatives; bis(pentabromophenyl)ether; diphenylether, octabromo-derivative. 3. It was concluded that in some cases, biomonitoring data are either lacking or scarce. This is rather surprising since the selection of the substances of the priority list was based on high tonnage, widespread use, extent of human exposure, and toxicological concern. The development of biomonitoring information could be helpful in assessing individual or population chemical exposure whatever the source and route, and would result in both more realistic and more accurate risk assessments.


Assuntos
Monitoramento Ambiental , Exposição Ocupacional , Medição de Risco/métodos , Acrilonitrila/análise , Estudos de Avaliação como Assunto , Humanos , Absorção Cutânea , Estireno/análise
18.
Comput Aided Surg ; 6(6): 340-51, 2001.
Artigo em Inglês | MEDLINE | ID: mdl-11954065

RESUMO

The purpose of Computer-Assisted Surgery (CAS) is to help physicians and surgeons plan and execute optimal strategies from multimodal image data. The execution of such planned strategies may be assisted by guidance systems. Some of these systems, called synergistic systems, are based on the cooperation of a robotic device with a human operator. We have developed such a synergistic device: PADyC (Passive Arm with Dynamic Constraints). The basic principle of PADyC is to have a manually actuated arm that dynamically constrains the authorized motions of the surgical tool held by the human operator during a planned task. Dynamic constraints are computed from the task definition, and are implemented by a patented mechanical system. In this paper, we first introduce synergistic systems and then focus on modeling and algorithmic issues related to the dynamic constraints. Finally, we describe a 6-degree-of-freedom prototype robot designed for a clinical application (cardiac surgery) and report on preliminary experiments to date. The experimental results are then discussed, and future work is proposed.


Assuntos
Procedimentos Cirúrgicos Cardíacos/instrumentação , Sistemas Homem-Máquina , Robótica/instrumentação , Cirurgia Assistida por Computador/instrumentação , Algoritmos , Desenho de Equipamento , Humanos , Projetos de Pesquisa , Cirurgia Assistida por Computador/métodos , Interface Usuário-Computador
19.
Chirurg ; 65(1): 74-6, 1994 Jan.
Artigo em Alemão | MEDLINE | ID: mdl-8149806

RESUMO

This report presents one case of rare benign intraosseus lipoma of the os calcis, which is analysed under the clinical, histopathologic and radiologic view. Not only the radiographic but also the computed tomographic findings has shown a typical picture of an intraosseous lipoma including a central calcification. In order to exclude a malignant transformation and as a prophylaxis of a pathological fracture the operative removal and bone graft transplantation is considered as therapy of choice.


Assuntos
Neoplasias Ósseas/cirurgia , Calcâneo/cirurgia , Lipoma/cirurgia , Neoplasias Ósseas/patologia , Calcâneo/patologia , Diagnóstico Diferencial , Humanos , Lipoma/patologia , Masculino , Pessoa de Meia-Idade , Estadiamento de Neoplasias
20.
Int J Comput Dent ; 6(3): 283-92, 2003 Jul.
Artigo em Inglês, Alemão | MEDLINE | ID: mdl-14601189

RESUMO

The Cerec method offers experienced users the possibility of accomplishing veneers chairside which are capable of meeting the highest functional and esthetic requirements. "Esthetic shaping" directly on the patient requires a good eye for color and form, and this requires training. The present report outlines the clinical procedure and presents three clinical cases step by step, specifically discussing the external staining technique with Pro-CAD blocks.


Assuntos
Cerâmica , Desenho Assistido por Computador , Facetas Dentárias , Adolescente , Adulto , Anodontia/terapia , Traumatismos em Atletas/terapia , Ciclismo/lesões , Cor , Porcelana Dentária/química , Planejamento de Prótese Dentária , Estética Dentária , Feminino , Humanos , Incisivo/anormalidades , Incisivo/lesões , Masculino , Coroa do Dente/lesões , Fraturas dos Dentes/terapia
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