RESUMO
Following reports of West Nile neuroinvasive disease in the north-eastern area of Italy in 2009, all blood donations dating from the period between 1 August and 31 October 2009 in the Rovigo province of the Veneto region were routinely checked to exclude those with a positive nucleic acid test for West Nile virus (WNV). Only one of 5,726 blood donations was positive (17.5 per 100,000 donations; 95% confidence interval (CI): 0.497.3). In addition, a selection of 2,507 blood donations collected during the period from 20 July to 15 November 2009 were screened by ELISA for IgG and IgM antibodies against WNV. A positive result was received for 94 of them. The positive sera were further evaluated using immunofluorescence and plaque reduction neutralisation test (PRNT), in which only 17 sera were confirmed positive. This corresponds to a prevalence of 6.8 per 1,000 sera (95% CI: 4.010.9). In a case-control study that matched each of the 17 PRNT-positive sera with four negative sera with the same date of donation and same donation centre, we did not find a significant association with age and sex of the donor; donors who worked mainly outdoors were significantly more at risk to have a positive PRNT for WNV.
Assuntos
Anticorpos Antivirais/sangue , Doadores de Sangue , Imunoglobulina G/sangue , Imunoglobulina M/sangue , Febre do Nilo Ocidental/epidemiologia , Vírus do Nilo Ocidental/isolamento & purificação , Adulto , Idoso , Anticorpos Antivirais/imunologia , Ensaio de Imunoadsorção Enzimática , Feminino , Imunofluorescência , Técnica de Placa Hemolítica , Humanos , Imunoglobulina G/imunologia , Imunoglobulina M/imunologia , Incidência , Itália/epidemiologia , Masculino , Pessoa de Meia-Idade , Testes de Neutralização , Prevalência , Estudos Retrospectivos , Sensibilidade e Especificidade , Estudos Soroepidemiológicos , Febre do Nilo Ocidental/diagnóstico , Febre do Nilo Ocidental/virologia , Vírus do Nilo Ocidental/imunologiaAssuntos
Bioprótese , Artéria Ilíaca/transplante , Ureter/cirurgia , Animais , Cães , Feminino , MasculinoRESUMO
Two cases of children treated for malignant tumor and who developed a severe lacticacidosis are reported. A 2 year-old girl and a 8 year-old boy were treated with chemotherapy, irradiation and multiple surgical procedures for nephroblastoma and lymphoma respectively. These two malnourished patients, under exclusive parenteral nutrition for two weeks without vitamin intakes, suddenly developed neurological, cardiovascular and digestive symptoms, associated with cytopenia and lacticacidosis. Injection of vitamin B1 only corrected these abnormalities within a few hours, proving the role of thiamine deficiency as the cause of the symptoms.