Work, family, and personal characteristics explain occupational and gender differences in work-family conflict among Japanese civil servants.

OBJECTIVES: A high level of work-family conflict (WFC) is an important risk factor for physical and mental health problems. Although individual work-related factors for WFC have been extensively studied, relatively little is known about whether occupation and gender affect WFC and how such effects might be generated. STUDY DESIGN: Cross-sectional study. METHODS: This study surveyed 3053 civil servants aged 20-65 years working in a local government in the west coast of Japan in 2003. Logistic regression analyses were used to examine whether there are occupational and gender differences in WFC and to clarify the factors underlying these differences. RESULTS: WFC was higher in professional and technical workers compared with other occupations for both men and women, with age-adjusted odds ratios (ORs) for these workers of 1.29 in men and 2.58 in women. In men, occupational differences in WFC disappeared after adjusting for work and family characteristics (OR = 1.15). In women, significant occupational differences remained in the final model, but after adjusting for work characteristics the adjusted OR for professional and technical workers was reduced to 1.69. Women were more likely than men to experience high WFC (OR = 2.52). After controlling for work characteristics, the gender difference was considerably reduced (OR = 1.68). CONCLUSIONS: Work characteristics play a fundamental role in the difference in WFC between not only occupational but also gender differences. Stressful work characteristics among professional and technical workers and among women in all work roles should be addressed to reduce occupational and gender differences in WFC in Japan.

Country specific associations between social contact and mental health: evidence from civil servant studies across Great Britain, Japan and Finland.

OBJECTIVES: Little is known about which component, such as social contact of social networks is associated with mental health or whether such an association can be observed across countries. This study examined whether the association between frequent social contact and mental health differs by composition (relatives or friends) and whether the associations are similar across three occupational cohorts from Great Britain, Japan, and Finland. STUDY DESIGN: Cross-sectional analysis of data from three prospective cohort studies. METHODS: Participants were civil servants of a prospective cohort study based in London (Men: n = 4519; Women: n = 1756), in the West Coast of Japan (Men: n = 2571; Women: n = 1102), and in Helsinki, Finland (Men: n = 1181; Women: n = 5633); we included the information on study variables which is complete. Mental health function was the study outcome, indicated by the total score from the Mental Health Component on the Short Form Health Survey36. Participants reported frequencies of contacts with their relatives or friends via a questionnaire. Age, marital status, and occupational position were treated as confounders in this study. RESULTS: Findings from multiple regression showed that the associations between social contact and mental health function were different depending on country of origin and gender. Among British or Japanese men, frequent contact with both friends and relatives was positively associated with their mental health function, while only social contact with friends was significantly associated with mental health of Finnish men. In women, the patterns of the associations between social contact and mental health were more distinctive: friends for Great Britain, relatives for Japan, and friends and relatives for Finland. These significant associations were independent of the confounders. CONCLUSIONS: Social contact was related to mental health of working people; however, culture and gender are likely to be tapped into.

Outdoor physical activity and its relation with self-reported health in Japanese children: results from the Toyama birth cohort study.

BACKGROUND: Few studies have examined trends in engagement in outdoor physical activity as children grow and whether changes in physical activity at different ages affect children's health. This study determined the preference for and frequency of physical activity among Japanese children from ages 6 to 12 years and investigated the effect of physical activity and of change in physical activity on children's self-reported health. METHODS: Data were from the prospective, longitudinal Toyama Birth Cohort Study, a total of 5238 children were followed at their age of 12 years. Preference for and frequency of outdoor physical activity were from the self-administered questionnaire. Self-reported health was from the Japanese version of Dartmouth Primary Care Co-operative project charts. RESULTS: Reporting liking and participating in outdoor physical activity at both ages 6 and 12 years were associated with higher likelihood of good self-reported health (Odds ratio 1.24 [95% CI: 1.03-1.50] for liking activity and OR = 1.27[1.08, 1.50] for participating in activity) compared with those who did not like or participate in this at only one or at neither age, after adjustment for lifestyle factors and body pain. The adjusted OR was 1.23 (95% CI: 0.97-1.56) for girls whose preference for liking outdoor physical activity was not changed at both ages compared with those whose preference changed. The OR was 1.47 (95% CI: 1.14-1.89) for boys who persisted in participating in the outdoor physical activity than those who did not persist. CONCLUSIONS: There is an association between a persistent expression of liking outdoor physical activity and self- reported health.

Conflicts between work and family life and subsequent sleep problems among employees from Finland, Britain, and Japan.

PURPOSE: Research on the association between family-to-work and work-to-family conflicts and sleep problems is sparse and mostly cross-sectional. We examined these associations prospectively in three occupational cohorts. METHODS: Data were derived from the Finnish Helsinki Health Study (n = 3,881), the British Whitehall II Study (n = 3,998), and the Japanese Civil Servants Study (n = 1,834). Sleep problems were assessed using the Jenkins sleep questionnaire in the Finnish and British cohorts and the Pittsburgh Sleep Quality Index in the Japanese cohort. Family-to-work and work-to-family conflicts measured whether family life interfered with work or vice versa. Age, baseline sleep problems, job strain, and self-rated health were adjusted for in logistic regression analyses. RESULTS: Adjusted for age and baseline sleep, strong family-to-work conflicts were associated with subsequent sleep problems among Finnish women (OR, 1.33 (95 % CI, 1.02-1.73)) and Japanese employees of both sexes (OR, 7.61 (95 % CI, 1.01-57.2) for women; OR, 1.97 (95 % CI, 1.06-3.66) for men). Strong work-to-family conflicts were associated with subsequent sleep problems in British, Finnish, and Japanese women (OR, 2.36 (95 % CI, 1.42-3.93), 1.62 (95 % CI, 1.20-2.18), and 5.35 (95 % CI, 1.00-28.55), respectively) adjusted for age and baseline sleep problems. In men, this association was seen only in the British cohort (OR, 2.02 (95 % CI, 1.42-2.88)). Adjustments for job strain and self-rated health produced no significant attenuation of these associations. CONCLUSION: Family-to-work and work-to-family conflicts predicted subsequent sleep problems among the majority of employees in three occupational cohorts.

Inactivation of bacteriophages by high levels of dissolved CO2.

We developed a system with high levels of dissolved CO2 for water disinfection. Bacteriophages MS2, Qbeta and phiX174 were selected as the inactivation targets. A relatively mild inactivation effect was observed on MS2 and Qbeta at different initial concentrations of dissolved CO2 at 0.3 MPa in 20-30 min. When the pressure was increased to 0.6 MPa, the inactivation of MS2 and Qbeta was differentially improved. However, this system was less effective for the inactivation of phiX174. The capsid surface property is a probable reason for the low inactivation of phiX174. The pH was not a key factor in the inactivation of bacteriophages; moreover, the results obtained using alternative gases (pressurized air and O2) indicated that only CO2 inactivated these bacteriophages. A comparison between the results of real time polymerase chain reaction (RT-PCR) and plaque assay showed that some RNA moved out from the capsid after treatment. Capsid damage by CO2 expansion was the likely mechanism of inactivation with our method.

Removal of hydrogen sulfide using palygorskite in a fixed bed adsorber.

This work describes the use of a novel palygorskite, a type of magnesium aluminium silicate clay possessing a high specific surface area and pore surface activity, as a low cost and highly efficient adsorbent for hydrogen sulfide (H(2)S) removal. Adsorption of H(2)S on palygorskite pretreated with acid or base was investigated in a fixed bed adsorber. The samples after base pretreatment had better dynamic adsorption performances than raw material and samples pretreated with acid. The H(2)S adsorption capacity decreased with an increase in inlet H(2)S concentration. This can be interpreted by the fact that H(2)S adsorption on the surface of palygorskite is chemisorption. The adsorption capacity increased from 25 to 50 °C, then decreased from 50 to 100 °C, which indicates that chemisorption took place and its better reaction temperature was around 50 °C.

Decolorization and mineralization of Oolong tea polyphenols in colored soft drink wastewater by photo Fenton reaction.

The decolorization and the mineralization of the colored soft drink wastewater including Oolong tea polyphenols by the photo Fenton reaction have been investigated. The decolorization of the colored soft drink wastewater including Oolong tea polyphenols by the photo Fenton reaction could be divided into 3 phases. Just after H2O2 was added to the solution, the color of the solution immediately increased from absorbance of 0.247 to 0.711 at the wavelength of 400 nm, which was defined as the 1st phase. Subsequently the significant decolorization by the photo Fenton reaction occurred at the 2nd phase. Finally, complete decolorization (the color attributed to the color of Fe3+) could be achieved in 180 min at the 3rd phase. The instantaneous and considerable color increase at the 1st phase could be attributed to the formation of intermediate colored compounds like quinones and soluble iron complexes produced by the Fenton reaction. About 95% mineralization of model colored soft drink wastewater with 229 mg L(-1) initial TOC concentration was achieved after 165 min.

Work-family conflicts and health behaviors among British, Finnish, and Japanese employees.

BACKGROUND: Work-family conflicts are associated with poor health. However, work-family conflicts and health behaviors have been little studied. PURPOSE: This study examined the associations of conflicts between paid work and family life with unhealthy behaviors among British, Finnish, and Japanese employees. METHOD: Data were derived from postal questionnaire surveys among 40 to 60 years old employees from three cohorts, the British Whitehall II Study (n = 3,397), the Finnish Helsinki Health Study (n = 4,958), and the Japanese Civil Servants Study (n = 2,901). Outcomes were current smoking, heavy drinking, physical inactivity, and unhealthy food habits. Work-family conflicts were measured with eight items. Age, marital status, and occupational class were adjusted for in logistic regression analyses. RESULTS: Work-family conflicts had few and inconsistent associations with unhealthy behaviors in all three cohorts. In the Finnish cohort, strong work-family conflicts were associated with current smoking among men. Women with strong conflicts had more often unhealthy food habits and were more often heavy drinkers than women with weaker conflicts. Likewise, British women with strong work-family conflicts were more often heavy drinkers. CONCLUSION: Although work-family conflicts were fairly prevalent in the examined cohorts, these conflicts had but few associations with the studied key health behaviors.

Independent histological risk factors for lymph node metastasis of superficial esophageal squamous cell carcinoma; implication of claudin-5 immunohistochemistry for expanding the indications of endoscopic resection.

Endoscopic resection is curative for superficial esophageal squamous cell carcinoma (ESCC) limited to the lamina propria. Endoscopic resection is not recommended for superficial ESCC invading muscularis mucosa or submucosa, however, because of the high frequency of lymph node metastasis (LNM) in such patients. Methods to more accurately predict LNM by analysis of endoscopically resected specimens are needed. Patients with superficial ESCC who underwent surgery without prior chemoradiotherapy (n= 110) were retrospectively examined to determine whether LNM correlated with immunohistochemical parameters and conventional histological parameters, including depth of invasion and vascular permeation. Cancer cell expression of claudins-1, 5, and 7, E-cadherin, beta-catenin, and matrix metalloproteinase 7 was evaluated. Univariate analysis revealed that LNM correlated with claudin-5 expression, but not any other immunohistochemical parameter examined. Multivariate analysis revealed three independent risk factors for LNM: aberrant claudin-5 expression in cancer cells (odds ratio; OR [95% confidence interval]= 4.61[1.44-14.77]), depth of submucosal invasion greater than 200 microm (3.55 [1.02-13.17]), and positive lymphatic permeation (3.34 [1.22-9.15]). LNM was found in one of 29 (3.4%) patients with none of these three risk factors, and in 32 of 81 (39.5%) patients with one or more of these risk factors. In superficial ESCC, routine analysis of claudin-5 expression in cancer cells together with depth of invasion and lymphatic permeation may be useful for predicting LNM and thereby reducing the number of patients undergoing additional surgery after successful endoscopic resection.

Mother employment status and nutritional patterns in Japanese junior high schoolchildren.

AIMS: To investigate associations between mothers' employment (full, part time and no employment) and nutrition habits (regularity of breakfast, snack, dinner, meal speed and portion size) in a sample of Japanese junior high schoolchildren, 12-13 years of age. METHODS: A total of 10 453 children aged 12-13 years from the Toyama birth cohort study (fourth phase) participated, of whom 8906 children (89% response rate) responded to all questions related to the examined variables. Nutrition habits consisted of breakfast, snack, dinner, meal speed and meal portion. Children's obesity/overweight was measured by body mass index (BMI). RESULTS: Fathers' employment had no effect on their children's nutrition patterns. Children of full-time employed mothers were the most likely to snack and to skip dinner. Children of part-time employed mothers ate larger meal portions, and those of non-employed mothers reported faster meal speeds. BMI was significantly (P<0.001) higher among children of full-time employed mothers (19.3), and lowest among non-employed mothers (19.00). Children of full-time employed mothers are more likely to be overweight, but not obese compared with other children. CONCLUSION: There was a strong relationship between mother's employment and nutrition patterns in this cohort of Japanese schoolchildren; special programs focused on children's nutrition patterns should take into account the mothers' employment status.

Application of bead array technology to simultaneous detection of human leucocyte antigen and human platelet antigen antibodies.

BACKGROUND: Detection of antibodies against human leucocyte antigens (HLA) and human platelet antigens (HPA) is crucial for patients refractory to platelet transfusion therapy. However, a reliable and high-throughput method for HLA cross-matching and detecting HPA antibodies has not yet been described. STUDY DESIGN AND METHODS: Immunocomplex capture fluorescence analysis (ICFA) was developed for high-throughput, simultaneous detection of HLA and HPA antibodies. Microarray beads were separately coupled with monoclonal antibodies specific for CD36, CD41, CD42b, CD49b, CD61 and HLA class I antigens. Platelets reacting with patient serum were lysed and the lysates were incubated with the bead mixture to specifically capture antigen-antibody complexes via the epitopes on platelet glycoproteins or HLA antigens. The beads capturing immunocomplexes were then subjected to flow cytometric analysis. RESULTS: Immunocomplex capture fluorescence analysis was validated using 50 serum samples containing HLA antibodies and 20 serum samples containing HPA antibodies. The method enabled the detection of all the HLA antibodies with a sensitivity comparable to that of the purified HLA antigen-coated pooled-bead assay (FlowPRA, One Lambda, Canoga Park, CA, USA). The method also enabled the detection of all the HPA antibodies with a sensitivity higher than that of the mixed passive haemagglutination. CONCLUSION: In this study, we developed a rapid, simple and reliable method for the simultaneous analysis of HLA and HPA antibodies. ICFA can also be used as an alternative to the lymphocyte cytotoxicity test for HLA cross-matching.

Analysis of body responses to an accelerating platform by the largest-Lyapunov-exponent method.

Various disciplines have benefited from the advent of high-performance computing in achieving practical solutions to their problems, and the area of health care is no exception to this. Non-linear signal-processing tools have been developed to understand the hidden complexity of the time series, and these will help clinicians in diagnosis and treatment. Postural study helps the elderly and people with a balancing problem due to various pathological conditions. In elderly subjects, falls are common and may result in injury. Correct postural balance is basic to well-being and it influences our daily life significantly. These postural signals are non-stationary; they may appear to be random in the time scale and it is difficult to observe the subtle changes for the human observer. Hence, more hidden information can be obtained from the signal using non-linear parameters. In this paper, ten young normal subjects are subjected to the balancing platform whose acceleration is gradually increased from 1 m/s2 to 5 m/s2 to study the postural response. The ankle front-back acceleration and ankle pitch angular velocity sensor data were studied using the largest Lyapunov exponent (LLE). The results show that for higher acceleration of the platform the ankle movement follows a particular rhythm, resulting in a lower Lyapunov exponent. During lower acceleration of the balancing platform, this value is higher because of the random movement of the ankle. In this work, the pattern of the body response was studied using LLE values for different accelerations using ankle data as the base signal for the normal subjects.

Hyperactive mutants of mouse D-aspartate oxidase: mutagenesis of the active site residue serine 308.

The role of Ser-308 of murine D-aspartate oxidase (mDASPO), particularly its side chain hydroxyl group, was investigated through the use of site-specific mutational analysis of Ser-308. Recombinant mDASPO carrying a substitution of Gly, Ala, or Tyr for Ser-308 was generated, and fused to either His (His-mDASPO), or glutathione S-transferase, His, and S (GHS-mDASPO) at its N-terminus. Wild-type His-mDASPO or GHS-mDASPO or their mutant derivatives were expressed in Escherichia coli and purified by affinity chromatography. All purified recombinant proteins had functional DASPO activity. The Gly-308 and Ala-308 mutants had significantly higher catalytic efficiency towards D-Asp and N-methyl-D-Asp, and a higher affinity for flavin adenine dinucleotide (FAD) compared to the wild-type enzyme. The Tyr-308 mutant had lower catalytic efficiency and binding capacity. These results suggest that the side chain hydroxyl group of a critical residue of mDASPO, Ser-308, down-regulates enzymatic activity, substrate binding, and FAD binding. This study provides information on the active site of DASPO that will considerably enhance our understanding of the biological significance of this enzyme.

Improvement of oxygen transfer efficiency in aerated ponds using liquid-film-assisted approach.

In aerated ponds, oxygen is generally supplied through either diffused or mechanical aeration means. Surface transfer and bubble transfer both contribute significantly to oxygen transfer in a diffused aeration system. In the present study, a liquid-film-forming apparatus (LFFA) is successfully developed on a laboratory scale to improve considerably the surface transfer via the unique liquid film transfer technique. The experimental results show that the volumetric mass transfer coefficient for LFFA alone is found to be as much as 5.3 times higher than that for water surface and that the total volumetric mass transfer coefficient for the liquid film aeration system increases by 37% in comparison with a conventional aeration system. Additionally, by tuning finely the structural parameters of the LFFA, it can also lead to high dissolved oxygen (DO) water with the DO percent saturation greater than 90%. More importantly, this result is accomplished by simply offering a single-pass aeration at a depth as shallow as 26 cm. As a result, the objective of economical energy consumption in aerated ponds can be realized by lowering the aeration depth without sacrificing the aeration efficiency. It is noteworthy that the data presented in this study are acquired either numerically or experimentally.

Enhancement of oxygen transfer efficiency in diffused aeration systems using liquid-film-forming apparatus.

Surface transfer and bubble transfer both contribute significantly to oxygen transfer in a diffused aeration system. In the present study, liquid-film-forming apparatus is successfully developed on a laboratory scale to improve considerably the surface transfer via the unique liquid film transfer technique. The experimental results show that the volumetric mass transfer coefficient for liquid-film-forming apparatus alone is found to be as much as 5.3 times higher than that for water surface and that the total volumetric mass transfer coefficient for liquid film aeration system increases by 37 % in comparison with conventional aeration system. Additionally, by tuning finely the structural parameters of the liquid-film-forming apparatus, it can also lead to high dissolved oxygen water with the dissolved oxygen percent saturation greater than 90 %. More importantly, this result is accomplished by simply offering a single-pass aeration at the depth as shallow as 26 cm. As a result, the objective of economical energy consumption in diffused aeration systems can be realized by lowering the aeration depth without sacrificing the aeration efficiency.

Can sleep disturbance influence changes in mental health status? Longitudinal research evidence from ageing studies in England and Japan.

BACKGROUND: Little is known about the role of sleep disturbance in relation to changes in depressive states. We used data obtained from the participants aged 65 and over in the English Longitudinal Study of Ageing (ELSA, waves four and five, N = 3108) and the Japan Gerontological Evaluation Study (JAGES, 2010 and 2013 sweeps, N = 7527) to examine whether sleep disturbance is longitudinally associated with older adults' patterns of depressive states. METHODS: We created four patterns of depressive states (non-case, recovered, onset, repeatedly depressive) by combining responses to the measures (scoring four or more on seven items from the Center for Epidemiological Studies Depression Scale for the ELSA participants and scoring five or more for the Geriatric Depression Scale-15 for the JAGES participants) obtained at the baseline and follow-up. Sleep disturbance was assessed through responses to three questions on sleep problems. Age, sex, partnership status, household equivalised income, alcohol and cigarette use, and physical function were treated as confounders in this study. Additionally, information on sleep medication was available in JAGES and was included in the statistical models. RESULTS: More ELSA participants were non-depressive cases and reported no sleep disturbances compared with the JAGES participants. Findings from multinomial logistic regression analysis showed that more sleep disturbance was associated with the onset group in ELSA (RRR = 2.37, 95% CI = 1.44-3.90) and JAGES (RRR = 2.41, 95% CI = 1.79-3.25) as well as the recovery (RRR = 3.42, 95% CI = 1.98-5.90, RRR = 2.71, 95% CI = 1.95-3.75) and repeatedly depressed group (RRR = 7.24, 95% CI = 3.91-13.40, RRR = 5.16, 95% CI = 3.82-6.98). CONCLUSIONS: Findings suggest that the association between sleep disturbance and depression in older adults is complex.