Control of mesenchymal stem cell phenotype and differentiation depending on cell adhesion mechanism.

Control of cell-matrix adhesion has become an important issue in the regulation of stem cell function. In this study, a maltose-binding protein (MBP)-linked basic fibroblast growth factor (FGF2)-immobilised polystyrene surface (PS-MBP-FGF2) was applied as an artificial matrix to regulate integrin-mediated signalling. We sought to characterise human mesenchymal-stem cell (hMSC) behaviour in response to two different mechanisms of cell adhesion; (i) FGF2-heparan sulphate proteoglycan (HSPG)-mediated adhesion vs. (ii) fibronectin (FN)-integrin-mediated adhesion. Heparin inhibited hMSC adhesion to PS-MBP-FGF2 but not to FN-coated surface. The phosphorylation of focal adhesion kinase, cytoskeletal re-organisation, and cell proliferation were restricted in hMSCs adhering to PS-MBP-FGF2 compared to FN-coated surface. Expression of MSC markers, such as CD105, CD90 and CD166, decreased in hMSCs expanded on PS-MBP-FGF2 compared to expression in cells expanded on FN-coated surface. hMSCs that were expanded on FN-coated surface differentiated into osteogenic and adipogenic cells more readily than those that were expanded on PS-MBP-FGF2. Furthermore, we characterised the N-linked glycan structures of hMSCs depending on the cell adhesion mechanism using mass spectrometry (MS)-based quantitative techniques. MS analysis revealed that 2,3-sialylated glycans, a potential marker of stem cell function, were more abundant on hMSCs expanded on FN-coated surface than on those expanded on PS-MBP-FGF2. Thus, the differentiation potential of hMSCs is controlled by the type of adhesion substrate that might provide an idea for the design of biomaterials to control stem cell fate. Elucidation of the glycan structure on the cell membrane may help characterise hMSC function.

Interfacial reaction and its influence on the mechanical properties of the ASZ short fibre-reinforced Al alloy composite.

The effect of interfacial reaction on the mechanical properties of the AC8A Al alloy reinforced with ASZ short fibres (ASZ/AC8A composite) was studied. In the ASZ/AC8A composite, the interfacial reaction was observed to proceed between the SiO2 binder layer and Mg of the matrix to form MgAl2O4 at the interface. Formation of MgAl2O4 was believed to enhance the interfacial bonding strength, resulting in the improved composite strength. However, the interfacial reaction in the ASZ/AC8A composite always took place at the expense of Mg in the matrix, resulting in the composite devoid of the Mg bearing precipitates such as Al2CuMg and Mg2Si. Interfacial reaction mechanisms were investigated for composites containing various Mg contents. The resultant mechanical properties of the composite were measured to determine the adequate amount of Mg within the composite. Microstructural changes of the composite were observed using transmission electron microscopy and differential scanning calorimetry to provide qualitative analyses on the experimental observations.