Bioaccumulation and health risk assessment of trace elements in Tilapia (Oreochromis mossambicus) from selected inland water bodies.

The presence of toxic trace elements (TEs) has resulted in a worldwide deterioration in freshwater ecosystem quality. This study aimed to analyze the distribution of TEs, including chromium (Cr), nickel (Ni), arsenic (As), mercury (Hg), cadmium (Cd), and lead (Pb), in water, sediment, and organs of Tilapia (Oreochromis mossambicus) collected from selected inland water bodies in Tamil Nadu, India. The water samples exhibited a range of concentrations for TEs: Cr varied from 0.014 to 5.193 µg/L, Ni ranged from 0.283 to 11.133 µg/L, As ranged from 0.503 to 1.519 µg/L, Cd from 0.001 to 0.616 µg/L, and Pb ranged from non-detectable (ND) to 6.103 µg/L. The concentrations of TEs in sediment were found to vary within the following ranges: 5.259 to 32.621 mg/kg for Cr, 1.932 to 30.487 mg/kg for Ni, 0.129 to 0.563 mg/kg for As, 0.003 to 0.011 mg/kg for Cd, ND to 0.003 mg/kg for Hg, and 0.404 to 1.575 mg/kg for Pb. The study found that the accumulation pattern of TE in fishes across all selected areas was liver > bone > gill > muscle. The organs had TE concentrations of Cr (ND-0.769 mg/kg), Ni (ND-1.053 mg/kg), As (0.002-0.080 mg/kg), Pb (ND-0.411 mg/kg), and Hg (ND-0.067 mg/kg), which was below the maximum residual limit prescribed by EC and FSSAI. The bioconcentration factor (BCF) of TEs exhibited a greater magnitude in comparison with the biota-sediment accumulation factor due to the higher concentration of TEs in fish and lower level in water. The assessment of both carcinogenic and non-carcinogenic risks suggests that the consumption of Tilapia from the study region does not pose any significant risks.

Biomonitoring of mercury and selenium in commercially important shellfish: Distribution pattern, health benefit assessment and consumption advisories.

This study aims to explore the concentrations of Se and Hg in shellfish along the Gulf of Mannar (GoM) coast (Southeast India) and to estimate related risks and risk-based consumption limits for children, pregnant women, and adults. Se concentrations in shrimp, crab, and cephalopods ranged from 0.256 to 0.275 mg kg-1, 0.182 to 0.553 mg kg-1, and 0.176 to 0.255 mg kg-1, respectively, whereas Hg concentrations differed from 0.009 to 0.014 mg kg-1, 0.022 to 0.042 mg kg-1 and 0.011 to 0.024 mg kg-1, respectively. Se and Hg content in bamboo shark (C. griseum) was 0.242 mg kg-1 and 0.082 mg kg-1, respectively. The lowest and highest Se concentrations were found in C. indicus (0.176 mg kg-1) and C. natator (0.553 mg kg-1), while Hg was found high in C. griseum (0.082 mg kg-1) and low in P. vannamei (0.009 mg kg-1). Se shellfishes were found in the following order: crabs > shrimp > shark > cephalopods, while that of Hg were shark > crabs > cephalopods > shrimp. Se in shellfish was negatively correlated with trophic level (TL) and size (length and weight), whereas Hg was positively correlated with TL and size. Hg concentrations in shellfish were below the maximum residual limits (MRL) of 0.5 mg kg-1 for crustaceans and cephalopods set by FSSAI, 0.5 mg kg-1 for crustaceans and 1.0 mg kg-1 for cephalopods and sharks prescribed by the European Commission (EC/1881/2006). Se risk-benefit analysis, the AI (actual intake):RDI (recommended daily intake) ratio was > 100%, and the AI:UL (upper limit) ratio was < 100%, indicating that all shellfish have sufficient level of Se to meet daily requirements without exceeding the upper limit (UL). The target hazard quotient (THQ < 1) and hazard index (HI < 1) imply that the consumption of shellfish has no non-carcinogenic health impacts for all age groups. However, despite variations among the examined shellfish, it was consistently observed that they all exhibited a Se:Hg molar ratio > 1. This finding implies that the consumption of shellfish is generally safe in terms of Hg content. The health benefit indexes, Se-HBV and HBVse, consistently showed high positive values across all shellfish, further supporting the protective influence of Se against Hg toxicity and reinforcing the overall safety of shellfish consumption. Enhancing comprehension of food safety analysis, it is crucial to recognize that the elevated Se:Hg ratio in shellfish may be attributed to regular selenoprotein synthesis and the mitigation of Hg toxicity by substituting Se bound to Hg.

Diffusion of organochlorine (OCPs) and cypermethrin pesticides from rohu (Labeo rohita) internal organs to edible tissues during ice storage: a threat to human health.

The migration of organochlorine pesticides (OCPs) and cypermethrin residues from internal organs to edible tissues of ice-held Labeo rohita (rohu) was investigated in this study. The liver (246 µg/kg) had the highest level of ∑OCP residues, followed by the gills (226 µg/kg), intestine (167 µg/kg), and muscle tissue (54 µg/kg). The predominant OCPs in the liver and gut were endosulfan (53-66 µg/kg), endrin (45-53 µg/kg), and dichloro-diphenyl-trichloroethane (DDT; 26-35 µg/kg). The ∑OCP residues in muscle increased to 152 µg/kg when the entire rohu was stored in ice, but they decreased to 129 µg/kg in gill tissues. On days 5 and 9, the total OCPs in the liver increased to 317 µg/kg and 933 µg/kg, respectively. Beyond day 5 of storage, total internal organ disintegration had led to an abnormal increase in OCP residues of liver-like mass. Despite a threefold increase in overall OCP residues by day 9, accumulation of benzene hexachloride (BHC) and heptachlor was sixfold, endrin and DDT were fourfold, aldrin was threefold, and endosulfan and cypermethrin were both twofold. Endosulfan, DDT, endrin, and heptachlor were similarly lost in the gills at a rate of 40%, while aldrin and BHC were also lost at 60 and 30%, respectively. The accumulation of OCP residues in tissues has been attributed to particular types of fatty acid derivatives. The study concluded that while pesticide diffusion to edible tissues can occur during ice storage, the levels observed were well below the allowable limit for endosulfan, endrin, and DDT.

Potentially toxic metals in seawater, sediment and seaweeds: bioaccumulation, ecological and human health risk assessment.

This study assesses the bioaccumulation, ecological, and health risks associated with potentially toxic metals (PTMs), including Pb, Hg, Cd, As, and Cr in Hare Island, Thoothukudi. The results revealed that the concentration of PTMs in sediment, seawater, and S. wightii ranged from 0.095 to 2.81 mg kg-1, 0.017 to 1.515 mg L-1, and 0.076 to 5.713 mg kg-1, respectively. The highest concentrations of PTMs were found in the S. wightii compared to seawater and sediment. The high bioaccumulation of Hg and As in S. wightii suggests that it can be used as a bioindicator for these elements in this region. The ecological risk indices, which include individual, complex, biological, and ecological pollution indices, suggest that Hare Island had moderate contamination with Hg and Cd. However, there are no human health risks associated with PTMs. This study examines the current ecological and health risks associated with PTMs and emphasizes the importance of regular monitoring.

De-novo exposure assessment of heavy metals in commercially important fresh and dried seafood: Safe for human consumption.

The heavy metals (HMs) in seafood are alarming due to their biomagnification in the food chain. The concentrations of As, Cd, Hg, Pb, Cr, and Ni in both fresh and dried fish were quantified, and the potential exposure and safe intake levels for human consumption were assessed by the European Commission (EC) and the Food Safety Standard Authority of India (FSSAI). HMs concentrations ranged from 0.003 mg/kg (Cr) to 2.08 mg/kg for (As) and 0.007 mg/kg (Hg) to 2.76 mg/kg (As). Cd, Hg, and Pb levels in fresh and dried fish were below the maximum residue limits (MRLs) set by the EC and FSSAI, which were 0.1 mg/kg, 0.5 mg/kg, and 0.3 mg/kg, respectively. Cr and As concentrations were also below the MRLs of 12 mg/kg and 76 mg/kg for aquatic products specified by FSSAI. The concentration of HMs in fresh and dried fish was found in the order of As > Cr > Ni > Pb > Cd > Hg and As > Cd > Cr > Ni > Pb > Hg, while the fresh and dried fishes contained HMs in the following order: E. areolatus > S. longiceps > L.lentjen > S. barracuda > E. affinis > S. javus and DA > DS > DR > DB > DSF. The metal pollution index (MPI) validates seafood is HMs free, while the single (Pi) and Nemerow integrated pollution index (Pnw) indicate that concentrations of Cd and As in fresh and dried fish have exceeded the threshold value. The target hazard quotient (THQ<1), hazard index (HI < 1), and target cancer risk (TCR<10-4) indicate that there are no non-carcinogenic and carcinogenic risks through the consumption of seafood and seafood products collected from the Tuticorin coast and marketed at the domestic and international levels. The preliminary findings emphasize the importance of formulating domestic legislation/government initiatives to promote seafood and its consumption. The attainment of this objective shall be facilitated by examining the levels of persistent organic pollutants (POPs) in seafood and evaluating its potential risk to consumers.

Accumulation potential of heavy metals at different growth stages of Pacific white leg shrimp, Penaeus vannamei farmed along the Southeast coast of Peninsular India: A report on ecotoxicology and human health risk assessment.

This study compared the heavy metal concentration in water, sediment, and shrimp at different growth stages of culture and subsequently evaluated the ecotoxicological and human health risk status. Total trace element concentration in the water, sediment and shrimp ranged from not detected (ND) (Hg) to 91.05 (Fe) µg/L, 0.01 (Hg) to 19, 246.33 (Fe) mg/kg, and ND (Hg) to 13.98 (Fe) mg/kg, respectively. Toxic metals such as, Cd, Hg, and Pb in shrimps ranged from ND to 2.11 mg/kg, ND to 0.158 mg/kg, ND to 0.088 mg/kg, and ND to 0.469 mg/kg, respectively. Toxic heavy metals at all the growth stages of shrimps (days of culture (DOC)-01 to DOC-90) were found below the maximum residual limit (MRL) of 0.5 mg/kg set by the European Commission (EC). Similarly, Cu, Zn, and As concentrations in shrimp were also far below the MRLs of 30 mg/kg, 100 mg/kg, and 76 mg/kg set by the World Health Organization and Food Safety and Standard Authority of India, respectively. The concentration of heavy metals increased from DOC-01 to DOC-90 and was positively correlated with the length and weight of the shrimps (p < 0.05). The risk assessment was estimated for both Indians and Americans and found no carcinogenic (lifetime cancer risk (LCR) < 10-4) and non-carcinogenic (THQ and TTHQ<1) health risks through consumption of shrimp cultured in this region. The hazard quotient (HQdermal < 1), hazard index (HI < 1), and LCR (<10-4) values of the heavy metals indicated that the dermal absorption might not be a concern for the local fishermen and marine fish/shrimp farmworkers. Water and sediment quality indices were applied to assess the surface water and sediment quality, and their results were found nil to low levels of heavy metal contamination at all the sampling sites. All heavy metals studied in sediments were < effect range low (ERL) and < threshold effect level (TEL), indicating no adverse biological effects on aquatic organisms. Therefore, regular monitoring of the shrimp aquaculture system throughout the crop will provide evidence of heavy metals bioaccumulation in shrimps. This research will provide baseline data to help farmers establish the optimal aquaculture practices and regulatory authorities to formulate legislation and strategies to reduce heavy metal biomagnification in shrimps from farm to fork.

Effect of additives in the shelflife extension of chilled and frozen stored Indian octopus (Cistopus indicus).

In this study, the effect of commercial additives viz. cafodos and altesa employed to treat Indian octopus (Cistopus indicus) was examined during chilled and frozen storage. Shelf lives of treated and untreated octopus in ice were 6 and 8 days, respectively in ice. Treated and untreated frozen octopus had a shelf life of 40 days. Autolytic and microbiological changes were not controlled by the additives, as evidenced through rapid reduction in non-protein nitrogen (NPN) and α-amino nitrogen (α-AN) compounds; as well as accumulation of water soluble ammoniacal nitrogen and total volatile base- nitrogen (TVB-N) compounds. Loss of texture and colour were the major quality defects noticed in treated octopus as a result of enhanced protein solubility. Therefore, the additives approved for use in octopus neither enhanced the shelf life nor improved the sensory quality.

Bioaccessibility of toxic heavy metals/metalloids in edible seaweeds: Exposure and health risk assessment.

This study assesses the health risk due to heavy metals/metalloids (HMs/Ms) in edible seaweeds (Caulerpa racemosa, Kappaphycus alvarezii, and Ulva lactuca) through an in vitro bioaccessibility study. The percentage of bioabsorbed HMs/Ms in unprocessed and processed C. racemosa, U. lactuca, and K. alvarezii ranged from 3 % to 46 %, 3 % to 42 %, and 3 % to 40 %, respectively. The levels of HMs/Ms in seawater, sediment, and seaweeds were below the levels recommended by the European Commission (EC) and World Health Organization/Food and Agriculture Organization (WHO/FAO). The maximum accumulation of HMs/Ms was found during monsoons and post-monsoon seasons, and Cd, Pb, Hg, Cr, As, and Pb were predominant in all the samples. Tukey's post hoc test and t-test confirmed that thermal processing significantly reduced HMs/Ms in seaweeds. On the basis of the bioabsorption of HMs/Ms, the TTHQ values were found to be < 1, and the LCR values were within the acceptable limit (10-06 to 10-04), indicating no carcinogenic risks through seaweeds.

Selenium and mercury concentration, Se/Hg molar ratio and risk-benefit assessment of marine fish consumption: Human health risks and protective role of Se against Hg toxicity.

This study aimed to explore the concentrations of Se and Hg in marine fish along the Gulf of Mannar (southeast coast of India) and to assess related risks and risk-based consumption limits for children, pregnant women, and adults. Se concentrations in pelagic and benthic fish ranged from 0.278 to 0.470 mg/kg and 0.203 to 0.294 mg/kg, respectively, whereas Hg concentrations ranged from 0.028 to 0.106 mg/kg and 0.026 to 0.097 mg/kg, respectively. Se and Hg contents in demersal fish (Nemipterus japonicus) were 0.282 and 0.039 mg/kg, respectively. The lowest and highest Hg concentrations in pelagic fish were found in Scomberomorus commersoni and Euthynnus affinis whereas the lowest and highest Se concentrations in benthic fish were found in Scarus ghobban and Siganus javus. Se concentrations in marine fishes were found in the following order: pelagic > demersal > benthic whereas Hg concentrations were found in the following order: pelagic > benthic > demersal. The presence of Se in fish was positively correlated with trophic level (TL) and size whereas that of Hg was weakly correlated with TL and habitat and negatively correlated with size. Se risk-benefit analysis, the AI/RDI (actual intake/recommended daily intake) ratio was > 100 % and the AI/UL (upper limit) ratio was < 100 %, indicating that all fish have sufficient levels of Se to meet daily requirements without exceeding the UL. Hg level was below the maximum residual limit (MRL) of 0.5 mg/kg for most fish but it was 1 mg/kg in E. affinis and Lethrinus lentjan. The target hazard quotient (THQ < 1) and hazard index (HI < 1) imply that the consumption of fish poses no noncarcinogenic health risks. However, all examined fish had a mean Se/Hg molar ratio > 1, indicating that human intake of fishwas rather safe relative to Hg content. Health benefit indexes (Se-HBV and HBVse) with high positive values in all fish supported the protective effect of Se against Hg toxicity, suggesting the overall safety of fish consumption. The high Se/Hg ratio in fish could be attributed to the replacement of Se bound to Hg, thereby suppressing Hg toxicity and maintaining normal selenoprotein synthesis. This insight is useful for a better understanding of food safety analysis.

Trace metals in commercial seafood products (canned, pickled and smoked): Comparison, exposure and health risk assessment.

This study used inductively coupled plasma and mass spectrometry, followed by microwave digestion, to assess the concentration of six trace metals (Cr, Ni, As, Cd, Hg, and Pb) in three canned products (tuna in oil [TIO], sardine in oil [SIO], and mackerel in oil [MIO]), two pickled products (prawn pickle [PP] and fish pickle [FP]), and one smoked product (masmin) collected from Tuticorin market, southern India. Trace metal (TM) levels in canned, pickled, and smoked fish varied from 0.01 to 1.48, 0.009 to 0.94, and 0.08 to 4.13 mg/kg, respectively. The concentration of TMs in the seafood was observed in the following order: As > Cr > Pb > Ni > Cd > Hg. Regarding their accumulation in the seafood products, masmin showed the highest levels followed by SIO, MIO, TIO, FP, and PP. The results revealed that smoked products showed higher TM concentrations, followed by canned and pickled products. The level of TMs in seafood products was below the maximum residue limit set by the European Commission (EC/1881/2006) and the Food Safety and Standards Authority of India (FSSAI). Risk assessment for consumer health implied that the evaluated target hazard quotient (THQ < 1), hazard index (HI < 1), and target cancer risk (TCR < 1.E-04) values did not exceed their guideline values and did not cause non-carcinogenic and carcinogenic health impacts through seafood product consumption. The pollution index (Pi) values of TMs in seafood products ranged from 0.01 to 0.7, with As and Cd showing the lowest and highest values, which were below 0.7, indicating their safety for consumption. The metal pollution index (MPI) for TMs in seafood ranged from 9.E-05 to 0.007, with the lowest and highest values associated with PP and TIO products. The MPI value for seafood products was below 1, signifying low TMs accumulation, suggesting the safety of seafood for consumption. The maximum seafood meal consumption limit (CRmm) was calculated, showing that adults can safely consume > 16 meals/month without chronic or acute toxicity. The results of this study suggest that the accumulation of TMs in the analyzed seafood product was below the health guidelines and pollution index values, making it suitable for smooth domestic and international export as well as safe consumption.

Spatial distribution and seasonal variation of pesticide residues in the sediment, seawater, and edible seaweeds: Environmental and human health risk assessment.

This study evaluates the distribution of pesticide residues (PRs) and assesses the ecological and human health risks posed by them from seawater, sediment and edible seaweeds in the Gulf of Mannar. Results showed that hexachlorocyclohexane, heptachlor, aldrin, endrin and endosulfan were the predominant PRs. The maximum concentration of PRs was observed in the monsoon and post-monsoon seasons. Their concentrations in sediment exceeded interim sediment quality guidelines, indicating significant risks to benthic organisms. Except for endrin, the presence of other organochlorine pesticides (OCPs) was below the maximum residual limit. Human health risk assessment revealed that the estimated daily intake of PRs through seaweeds was below the acceptable daily intake. The hazard quotient and hazard ratio were <1, implying that the study seaweeds do not pose significant health risks. The presence of endrin in edible seaweeds, however, indicates safety concerns for consumers and the continued use of banned OCPs in agriculture.

First report on the exposure and health risk assessment of organochlorine pesticide residues in Caulerpa racemosa, and their potential impact on household culinary processes.

Seaweeds are widely consumed as natural seafood in various Asian countries. Chemical contaminants, such as pesticide residues (PRs), can contaminate it due to its high bio-accumulation nature. Limited research exists on the presence of PRs in edible seaweeds, their decrease in levels during cooking processes, and the evaluation of hazard indices and associated health risks to humans. This study investigated the effects of different cooking methods on the levels of organochlorine pesticides in Caulerpa racemosa seaweed. It also assessed the potential health risks associated with consuming seaweed by estimating daily intake, hazard quotient, and hazard index. The PRs were reduced after different cooking methods. The impact of thermal cooking on PRs in C. racemosa was found to be notably beneficial. The PRs decreased following MWC, boiling, and steam cooking. Several PRs were analyzed, and endrin, DDT, endosulfan, and cypermethrin were found to be the most prevalent. The HQ and HI values for raw and cooked seaweeds were found to be below one, suggesting that the PRs in C. racemosa pose no risk to consumers of seaweed. In summary, thermal cooking proves to be an efficient method for minimizing PRs, while the cooking of seaweeds ensures a high level of safety during consumption.

Effect of household culinary processes on organochlorine pesticide residues (OCPs) in the seafood (Penaeus vannamei) and its associated human health risk assessment: Our vision and future scope.

Food safety is crucial in today's competitive trading market, as it directly affects human health and promotes seafood exports. The effects of thermal processing (boiling, frying, grilling, and microwave cooking) on pesticide residues (PR) in P. vannamei were assessed. The PR in raw and processed shrimp ranged from 0.007 to 0.703 µg/kg for uncooked/raw, not detected (ND) to 0.917 µg/kg for boiled, ND to 0.506 µg/kg for fried, ND to 0.573 µg/kg for grilled and ND to 0.514 µg/kg for microwave cooked shrimps. The Endrin, endosulfan sulfate, and heptachlor were predominant PR found in the raw and processed shrimp. The PR content in raw and cooked shrimps were below the maximum residue limits (MRL) set by the Codex Alimentarius Commission (2021) and the European Commission (86/363/1986 and 57/2007). The estimated daily intake (EDI) of PR from raw and processed shrimps were below the ADI prescribed by CAC. The hazard quotient (HQ) and hazard ratio (HR) values were <1, indicating no non-carcinogenic or carcinogenic health implications through shrimp consumption. The estimated maximum allowable shrimp consumption rate (CRlim) suggests an adult can eat >100 shrimp meals/month, which is over the USEPA's (2000)recommendation of >16 meals/month without health issues. The Effect of thermal processing was detected in the following order: boiling < grilling < frying < microwave cooking. The processing factor (PF < 0.7), paired t-test (t < 0.05), Tukey post hoc (p < 0.05) test, Bray-Curtis similarity index, and matrix plot exhibited that all the four thermal processing methods have a considerable impact on pesticides in the processed shrimps. But frying (59.4%) and microwave cooking (60.3%) reduced PR far beyond boiling (48.8%) and grilling (51.3%). Hence, we recommend frying and microwave processing are better methods for minimizing PR in seafood than boiling or grilling.

Prevalence of biofilm forming Salmonella in different seafood contact surfaces of fishing boats, fish landing centres, fish markets and seafood processing plants.

The prevalence of biofilm forming Salmonella on different seafood contact surfaces was investigated. Out of 384 swab samples, 16.14 % and 1 % were confirmed biochemically and molecularly as Salmonella respectively. One out of four isolates was from the boat deck, and three were from the seafood processing plant. Salmonella was more prevalent in January, June, and September months. Different assays investigated the biofilm forming ability of isolates. Two out of four isolates have shown strong biofilms, and the others were moderate biofilm formers by microtitre plate assay. In the CRA assay, three isolates showed 'rdar' morphotype, and one showed 'bdar' morphotype. All isolates were positive for gcpA gene (~1700 bp), a critical gene found in Salmonella biofilms. The microbial load of Salmonella biofilms on different contact surfaces were determined, stainless steel and HDPE were found prone to biofilms. With this, a suitable mechanism shall be formulated to control the biofilms of Salmonella.

Bioaccumulation of organochlorine pesticide residues (OCPs) at different growth stages of pacific white leg shrimp (Penaeus vannamei): First report on ecotoxicological and human health risk assessment.

Pesticide residues (PRs) in farmed shrimps are concerning food safety risks. Globally, India is a major exporter of pacific white leg shrimp (P. vannamei). This study was undertaken to analyze PRs in the water, sediments, shrimps, and feed at different growth stages to evaluate the ecotoxicological and human health risks. PRs in the seawater and sediments ranged from not detected (ND) to 0.027 µg/L and 0.006-12.39 µg/kg, and the concentrations were within the maximum residual limits (MRLs) and sediment quality guidelines prescribed by the World Health Organization and Canadian Environment Guidelines, respectively. PRs in shrimps at three growth stages viz. Postlarvae, juvenile, and adults, ranged from ND to 0.522 µg/kg, below the MRLs set by Codex Alimentarius Commission and European Commission. Most of the PRs in water, sediments, and shrimps did not vary significantly (p > 0.05) from days of culture (DOC-01) to DOC-90. The hazard quotient (HQ) and hazard ratio (HR) were found to be < 1, indicating that consumption of shrimps has no noncarcinogenic and carcinogenic risks. PRs in shrimp feed ranged from ND to 0.777 µg/kg and were found to be below the MRLs set by EC, which confirms that the feed fed is safe for aquaculture practices and does not biomagnify in animals. The risk quotient (RQ) and toxic unit (TU) ranged from insignificant level (ISL) to 0.509 and ISL to 0.022, indicating that PRs do not pose acute and chronic ecotoxicity to aquatic organisms. The study suggested no health risk due to PRs in shrimps cultured in India and exported to the USA, China, and Japan. However, regular monitoring of PRs is recommended to maintain a sustainable ecosystem.

Trace element concentrations in the organs of fish along the southeast coast of India.

Trace element pollution in the marine system is a global concern as the exposure of marine organisms to this pollution results in bioaccumulation and further transfer of the trace elements to humans through food chain. In the present study, the distribution of trace elements, namely chromium, cobalt, nickel, iron, copper, zinc, arsenic, cadmium, mercury, and lead, in gills, bone, liver, and muscle of eight commercially important fish collected along the southeast coast of India was analyzed using an inductively coupled plasma mass spectrometer. The liver was the main organ of accumulation for copper, zinc, arsenic, cadmium, and mercury; bone for chromium, cobalt, and lead; gills for copper; and muscle for arsenic and mercury. The concentration of toxic trace elements such as arsenic, cadmium, mercury, and lead in the edible portion of fish was lower than the recommended International Legislation limits, indicating that the fish of this region are safe for consumption.

Pesticides contamination in the Thamirabarani, a perennial river in peninsular India: The first report on ecotoxicological and human health risk assessment.

This study evaluates the distribution of pesticides and assesses the ecological and human health risks associated with pesticide residues concentration in the Thamirabarani River, the only perennial river in Tamil Nadu, India. Observed a variation in the pesticide concentration in the water (not detected (ND)-31.69 µg/L), sediments (ND-14.77 µg/kg), and fish (0.02-26.05 µg/kg). Endosulfan, aldrin, and endrin were the predominant organochlorine pesticides present in water, sediments, and fish. The average concentration of pesticides (except endosulfan) in water and sediments was found to be below the acceptable threshold as per the water and sediment quality guidelines, posing no ecological hazard to aquatic organisms. The calculated risk quotient and toxic unit (0.1 > TU/RQ ≤ 1) represent low-to-medium acute and chronic toxicity to the aquatic organisms inhabiting the river basin. The average concentration of pesticides in fish (Labeorohita) was also below the maximum residual limits set by the Codex Alimentarius Commission (CAC). However, the calculated daily intakes of endosulfan, aldrin, and endrin were above the CAC-acceptable daily intake guidelines. The human health risk assessment showed that children and adults exposed to pesticides in water and sediments through ingestion and dermal contact could have higher cancer risks (CR > 10-4) than inhalation. This study recommends implementing effective and routine pollution management schemes to avoid pesticide threats to aquatic and human health.

Dietary intake of trace elements from commercially important fish and shellfish of Thoothukudi along the southeast coast of India and implications for human health risk assessment.

The concentrations of eight trace elements (chromium, cobalt, copper, zinc, arsenic, cadmium, mercury and lead) in14 commercially important fish and shellfish collected from Thoothukudi along the southeast coast of India was investigated using inductively coupled plasma mass spectrometry in order to assess the health risks associated with their consumption. The concentration of trace elements ranged from 0.001 to 39.5 µg/g. The estimated weekly intake of cadmium in seven fish and shellfish (0.0081-0.0996 mg/kg body weight) were above the provisional tolerable weekly intake set by the Joint FAO/WHO Expert Committee on Food Additives. The risk assessment analysis indicated that there was non- carcinogenic risk upon lifetime consumption of rock crab, C. natator (TTHQ >1) and carcinogenic risks upon lifetime consumption of S. jello, P. semisulcatus, P. sanguinolentus C. natator, Uroteuthis duvaceli, Sepia pharaonis and Cistopus indicus due to cadmium exposure indicating a potential health risk to the exposed consumers.

Human health risk assessment of heavy metals in aquatic sediments and freshwater fish caught from Thamirabarani River, the Western Ghats of South Tamil Nadu.

Industrialization and advancements in agriculture are increasingly the cause of environmental concerns and need to be addressed. This study was carried out at four sampling sites, viz. Manimuthar (site 1), Tirunelveli (site 2), Srivaikuntam (site 3), and Authoor (site 4), along the Thamirabarani river. The heavy metal concentration in fish and sediments were ranged from 0.001 to 9.505 mg kg-1 and 0.294 to 106.25 mg kg-1. The lifetime cancer risk (LCR) values for children and adults were found to be above the acceptable threshold value (ATV) and children were found to be more susceptible to health risks. For all sites except site 4, the LCR of toxic metals except Cd were within the ATV (10-6-10-4); the value of Cd was above the ATV, and poses a high cancer risk to the downstream inhabitants. This study advises routine heavy metal monitoring on aquatic organisms along these sites to implement regulatory standards.

Concentrations of trace elements in the organs of commercially exploited crustaceans and cephalopods caught in the waters of Thoothukudi, South India.

Concentrations of trace elements (arsenic, cadmium, mercury, and lead) in tissues (muscle, gills, and digestive gland) of three commercially exploited crustaceans (Portunus sanguinolentus, Charybdis natator, and Penaeus semisulcatus) and three cephalopods (Doryteuthis sibogae, Sepia pharaonis, and Cistopus indicus) were examined. The animals were captured in the waters of Thoothukudi, and the tissues of six individuals of each species were analyzed using ICP-MS. The highest concentrations of arsenic (16.5 µg/g) and mercury (0.052 µg/g) were recorded in the digestive gland of C. natator, and cadmium (69.9 µg/g) and lead (0.351 µg/g) in the digestive gland of S. pharaonis. The edible portion of the crustaceans and cephalopods contained lower concentrations of trace elements, and these were below allowable limits set by the European Union. The edible parts of the investigated samples are safe for human consumption, but accidental contamination of the edible tissues with material from the digestive glands could lead to concerns relating to metal toxicity.