1.
Nucleosides Nucleotides Nucleic Acids
; 32(12): 670-81, 2013.
Artigo
em Inglês
| MEDLINE
| ID: mdl-24328564
RESUMO
5'-deoxy-5'-hydrazinylguanosine was incorporated into the 5'-termini of RNA transcripts using T7 RNA polymerase. Transcriptions provided 5'-hydrazinyl-RNA that was readily labeled and purified. The use of fluorophore-labeled material was validated in an endoribonuclease activity assay.
Assuntos
Bacteriófago T7/enzimologia , RNA Polimerases Dirigidas por DNA/metabolismo , Guanosina/análogos & derivados , Hidrazinas/química , RNA/química , Proteínas Virais/metabolismo , Sequência de Bases , Fluoresceína-5-Isotiocianato/química , Corantes Fluorescentes/química , Guanosina/metabolismo , Hidrazinas/metabolismo , Dados de Sequência Molecular , RNA/genética , RNA/metabolismo , Transcrição Gênica
2.
Chem Commun (Camb)
; (33): 4980-1, 2009 Sep 07.
Artigo
em Inglês
| MEDLINE
| ID: mdl-19668822
RESUMO
We have developed simple, aqueous strategies, that avoid the use of protecting groups and chromatography, for the preparation of a series of 5'-substituted guanosine derivatives.