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1.
J Asian Nat Prod Res ; 23(2): 103-109, 2021 Feb.
Artigo em Inglês | MEDLINE | ID: mdl-32024382

RESUMO

Two new benzoic acid derivatives (1-2), together with four known compounds (3-6) have been isolated from the n-BuOH soluble fraction of ethanolic extract from Ailanthus altissima. The gross structures of the new compounds were deduced by detailed spectroscopic analysis including HRESIMS and 1D/2D NMR spectroscopy. The stereochemistry of 1 was determined by modified Mosher's method. All compounds were evaluated for their neuroprotective effects against H2O2-induced oxidative stress in human neuroblastoma SH-SY5Y cells and none of them displayed obvious neuroprotective activities. [Formula: see text].


Assuntos
Ailanthus , Ácido Benzoico , Peróxido de Hidrogênio/farmacologia , Estrutura Molecular , Extratos Vegetais
2.
Bioorg Chem ; 95: 103545, 2020 01.
Artigo em Inglês | MEDLINE | ID: mdl-31927316

RESUMO

A phytochemical study on the roots of Daphne genkwa yielded seven new guaiane-type sesquiterpenoids (1-7), daphne A-G. Their structures were elucidated through comprehensive spectroscopic analyses. The absolute configurations were determined by comparison between experimental electronic circular dichroism (ECD) and calculated ECD spectra via time-dependent density functional theory (TDDFT) and the modified Mosher's method. Furthermore, all isolates were evaluated for their neuroprotective activities against H2O2-induced injury in human neuroblastoma SH-SY5Y cells. Among them, compounds 1 (78.42%) and 4 (79.34%) exhibited potent neuroprotective effects against H2O2-induced neurotoxicity at 25 µM. Further Annexin V-FITC/propidium iodide (PI) doubling staining exhibited that the neuroprotective effects of compounds 1 and 4 appeared to be mediated via suppressing cell apoptosis. Flow cytometry assays also proved that compounds 1 and 4 could attenuate mitochondrial dysfunction in SH-SY5Y cells.


Assuntos
Daphne/química , Descoberta de Drogas , Fármacos Neuroprotetores/farmacologia , Raízes de Plantas/química , Sesquiterpenos/farmacologia , Linhagem Celular Tumoral , Humanos , Mitocôndrias/efeitos dos fármacos , Fármacos Neuroprotetores/química , Fármacos Neuroprotetores/isolamento & purificação , Sesquiterpenos/química , Sesquiterpenos/isolamento & purificação
3.
J Nat Prod ; 82(6): 1510-1517, 2019 06 28.
Artigo em Inglês | MEDLINE | ID: mdl-31150241

RESUMO

Chromatographic purification of the roots of Daphne genkwa led to 11 new guaiane-type sesquiterpenoids (1-11), named genkwanoids A-K, and six known analogues (12-17). A comprehensive set of spectroscopic methods including IR, UV, HRESIMS, and 1D/2D NMR were used to elucidate the structures and relative configurations of 1-11. The absolute configurations were determined by the optical rotation calculations and the modified Mosher's method. The possible biosynthetic pathways for 1-11 are proposed. Furthermore, the neuroprotective effects of 1-17 on H2O2-induced damage in human neuroblastoma SH-SY5Y cells were screened using an MTT assay. Compounds 9, 10, and 16 exhibited moderate neuroprotective effects, with survival rates of 79.34%, 79.94%, and 75.64% after treatment with 12.5 µM, values that were comparable to the positive control, Trolox (78.65%). Furthermore, annexin V-FITC/PI staining of cells treated with 9, 10, and 16 showed that the neuroprotective effects of these compounds may arise from inhibiting cell apoptosis.


Assuntos
Morte Celular/efeitos dos fármacos , Daphne/química , Fármacos Neuroprotetores/farmacologia , Raízes de Plantas/química , Sesquiterpenos de Guaiano/química , Sesquiterpenos/farmacologia , Humanos , Estrutura Molecular , Neuroblastoma , Fármacos Neuroprotetores/química , Fármacos Neuroprotetores/isolamento & purificação , Sesquiterpenos/química , Sesquiterpenos/isolamento & purificação
4.
Fitoterapia ; 141: 104448, 2020 Mar.
Artigo em Inglês | MEDLINE | ID: mdl-31816344

RESUMO

Five undescribed macrocarpene-type sesquiterpenes (1-5), along with a known analogue (6) were isolated from the crude extract of stigma maydis. The structures of these compounds were elucidated based on comprehensive spectroscopic analyses together with quantum chemical calculations of 13C NMR data and electronic circular dichroism (ECD) curves. All isolated compounds were tested for their neuroprotective effects against the injury of human neuroblastoma SH-SY5Y cells induced by H2O2. Among them, compounds 3 (65.89%) and 5 (64.38%) showed moderate neuroprotective activity at 50 µM.


Assuntos
Flores/química , Fármacos Neuroprotetores/isolamento & purificação , Fármacos Neuroprotetores/farmacologia , Extratos Vegetais/farmacologia , Sesquiterpenos/isolamento & purificação , Sesquiterpenos/farmacologia , Zea mays/química , Linhagem Celular Tumoral , China , Medicamentos de Ervas Chinesas/química , Medicamentos de Ervas Chinesas/isolamento & purificação , Medicamentos de Ervas Chinesas/farmacologia , Humanos , Peróxido de Hidrogênio , Estrutura Molecular , Neuroblastoma/tratamento farmacológico , Fármacos Neuroprotetores/química , Extratos Vegetais/química , Sesquiterpenos/química
5.
Fitoterapia ; 143: 104559, 2020 Jun.
Artigo em Inglês | MEDLINE | ID: mdl-32199958

RESUMO

Four undescribed oxylipin vanillyl acetals with four stereogenic carbons were isolated from the herbs of Solanum lyratum. A comprehensive set of spectroscopic methods were used to elucidate the structures and relative configurations of 1-4. The absolute configurations of the naturally occurring compounds are assigned as 7S, 9'S, 10'S, 11'R at the site of six-membered cyclic acetal attachment by electronic circular dichroism (ECD) calculations and the modified Mosher's method. Compounds 1 and 3 displayed moderate selective inhibition against Hep3B and HepG2 cells, respectively. Further Annexin V-FITC/PI staining assay revealed that 1 and 3 might have inhibitory effects on hepatoma cells through induction of apoptosis.


Assuntos
Acetais/farmacologia , Antineoplásicos Fitogênicos/farmacologia , Oxilipinas/farmacologia , Solanum/química , Acetais/isolamento & purificação , Antineoplásicos Fitogênicos/isolamento & purificação , Apoptose , China , Células Hep G2 , Humanos , Estrutura Molecular , Oxilipinas/isolamento & purificação , Compostos Fitoquímicos/isolamento & purificação , Compostos Fitoquímicos/farmacologia
6.
Phytochemistry ; 175: 112361, 2020 Jul.
Artigo em Inglês | MEDLINE | ID: mdl-32289598

RESUMO

Seven undescribed terpenylated coumarins, named altissimacoumarin I-O, together with seven known compounds, altissimacoumarin C, altissimacoumarin E, altissimacoumarin G, altissimacoumarin H, puberulin, 7-(3-Methyl-2-butenyloxy)-6-methoxycoumarin and artelin were isolated from the root bark of Ailanthus altissima (Mill.) Swingle. Their structures were elucidated by comprehensive spectra data analysis, NMR calculation, DP4+ analysis and ACD/Structure Elucidator software simulation. The absolute configurations of altissimacoumarins K, L, M and N were determined by modified Mosher's method. All isolates were tested for their cytotoxic effect against two hepatoma carcinoma cell lines (HepG2, Hep3B). Altissimacoumarin C exhibited moderate cytotoxic effect against Hep3B cells, with IC50 of 45.21 µM.


Assuntos
Ailanthus , Cumarínicos , Casca de Planta , Extratos Vegetais
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