RESUMO
The combined use of phage and antibiotics can show synergistic antimicrobial effects, so-called phage-antibiotic synergy (PAS). Here, we screened and examined PAS against Pseudomonas aeruginosa in vitro. Testing four different phages infecting P. aeruginosa, phage KPP22 classified within the family Myoviridae genus Pbunavirus showed PAS with the widest range of antibiotics, and showed PAS with anti-Pseudomonas drugs such as piperacillin and ceftazidime. Thus, evidence suggests that the combined use of phage and antibiotics is a promising therapeutic strategy against P. aeruginosa infections, with consideration needed regarding the optimal selection and adequate application timing of these phages and antibiotics.
Assuntos
Antibacterianos/farmacologia , Ceftazidima/farmacologia , Myoviridae/fisiologia , Piperacilina/farmacologia , Fagos de Pseudomonas/fisiologia , Pseudomonas aeruginosa/efeitos dos fármacos , Pseudomonas aeruginosa/fisiologia , Humanos , Testes de Sensibilidade Microbiana , Myoviridae/classificação , Terapia por Fagos , Fagos de Pseudomonas/classificação , Fagos de Pseudomonas/genética , Pseudomonas aeruginosa/virologiaRESUMO
Bacteriophages (phages) belonging to the family Podoviridae genus N4-like viruses have been used as therapeutic agent in phage therapy against Pseudomonas aeruginosa infections. P. aeruginosa phage KPP21 was isolated in Japan, and phylogenetically investigated the phages belonging to this viral genus. Morphological and genetic analyses confirmed that phage KPP21 belongs to the family Podoviridae genus N4-like viruses. Moreover, phylogenetic analyses based on putative DNA polymerase and major virion protein showed that P. aeruginosa phages belonging to the genus N4-like viruses are separated into two lineages and that phage KPP21 is in the same clade as phage LUZ7.
Assuntos
DNA Viral/genética , Podoviridae/classificação , Fagos de Pseudomonas/classificação , Pseudomonas aeruginosa/virologia , Composição de Bases , Mapeamento Cromossômico , Genoma Viral , Japão , Microscopia Eletrônica de Transmissão , Dados de Sequência Molecular , Filogenia , Podoviridae/isolamento & purificação , Podoviridae/ultraestrutura , Infecções por Pseudomonas/virologia , Fagos de Pseudomonas/genética , Fagos de Pseudomonas/isolamento & purificação , Fagos de Pseudomonas/ultraestruturaRESUMO
INTRODUCTION: Bilateral renal cell carcinomas with different histological types are rare. We report herein the first description of bilateral renal carcinomas with clear cell renal cell carcinoma and mucinous tubular and spindle cell carcinoma occurring synchronously. CASE PRESENTATION: A 62-year-old man was referred to our hospital with bilateral renal tumors. The tumors on each side showed different findings from both contrast-enhanced computed tomography and magnetic resonance imaging. The tumors were partially resected. Histopathological and immunohistochemical examination of the left renal tumor diagnosed clear cell renal carcinoma. Histopathological and immunohistochemical examination of the right renal tumor diagnosed mucinous tubular and spindle cell carcinoma. CONCLUSION: We encountered a case with clear cell renal cell carcinoma and mucinous tubular and spindle cell carcinoma occurring simultaneously in bilateral kidneys.
RESUMO
Pseudomonas aeruginosa phages belonging to the family Podoviridae are one of the well-characterized phage groups. In this study, a novel P. aeruginosa phage, KPP25, was isolated and characterized. Phage KPP25's morphology was indicative of the family Podoviridae; however, analyses of the whole genome and the virion proteins suggested that it did not belong to any of the known podophage genera. Based on these analyses, phage KPP25 appears to be a novel podophage infecting P. aeruginosa.
Assuntos
DNA Viral/química , DNA Viral/genética , Genoma Viral , Podoviridae/genética , Fagos de Pseudomonas/genética , Pseudomonas aeruginosa/virologia , Ordem dos Genes , Humanos , Microscopia Eletrônica de Transmissão , Dados de Sequência Molecular , Podoviridae/isolamento & purificação , Podoviridae/ultraestrutura , Fagos de Pseudomonas/isolamento & purificação , Fagos de Pseudomonas/ultraestrutura , Análise de Sequência de DNARESUMO
Bacteriophage (phage) therapy is expected to become an alternative therapy for Pseudomonas aeruginosa infections. P. aeruginosa phage KPP23 is a newly isolated phage belonging to the family Siphoviridae and may be a therapeutic phage candidate. We report its complete genome, which comprises 62,774 bp of double-stranded DNA containing 95 open reading frames.