An unusual protein-protein interaction through coupled unfolding and binding.

Aptides, a novel class of high-affinity peptides, recognize diverse molecular targets with high affinity and specificity. The solution structure of the aptide APT specifically bound to fibronectin extradomain B (EDB), which represents an unusual protein-protein interaction that involves coupled unfolding and binding, is reported. APT binding is accompanied by unfolding of the C-terminal ß strand of EDB, thereby permitting APT to interact with the freshly exposed hydrophobic interior surfaces of EDB. The ß-hairpin scaffold of APT drives the interaction by a ß-strand displacement mechanism, such that an intramolecular ß sheet is replaced by an intermolecular ß sheet. The unfolding of EDB perturbs the tight domain association between EDB and FN8 of fibronectin, thus highlighting its potential use as a scaffold that switches between stretched and bent conformations.

A Study on the Preliminary Validity Analysis of Korean Firefighter Job-Related Physical Fitness Test.

The purpose of this study is to revise and modify the firefighter job-related performance tests from overseas to implement into the circumstances in Korea, examine its validity by analyzing the level of association between the test employed in the ongoing firefighter selection process, and propose a Korean firefighter job-related physical fitness test. Therefore, a modified version of Candidate Physical Ability Test (CPAT) from the United States firefighter selection process was conducted on 28 male firefighter officer candidates. Recorded results from VO2max, heart rate, blood lactate, anaerobic power test, and the ongoing Korean firefighter physical fitness test were analyzed to assess the validity of the modified test. IBM SPSS Statistics Ver. 27.0 was employed for the data correlation analysis in different fitness factors and the total circuit physical test time. The results revealed the proposed modified firefighter job-related physical fitness test showed significant correlation with VO2max (r = −0.450, p < 0.05), METS (r = −0.735, p < 0.01) recovery lactate over 15 min (r = −0.460, p < 0.05), peak power (r = −0.484, p < 0.05), average power (r = −0.647, p < 0.01), and in the ongoing firefighter physical fitness test, grip strength (r = −0.709, p < 0.01), lower back strength (r = −0.681, p < 0.01), standing long jump (r = −0.618, p < 0.01), sit-ups (r = −0.397, p < 0.05), and shuttle run (r = −0.523, p < 0.01). Fitness factors including VO2max, recovery lactate, anaerobic power, muscular strength, and so forth known to play a crucial role in firefighting operations were also shown to be important in the modified firefighter job-related physical fitness test. However, we suggest that studies with a larger sample size are needed in order to generalize our findings.

Relations of Conspicuous Consumption Tendency, Self-Expression Satisfaction, and SNS Use Satisfaction of Gen Z through SNS Activities.

The purpose of this study was to analyze the relations of conspicuous consumption tendency, self-expression satisfaction, and SNS use satisfaction of Gen Z through SNS activities. For a week from 17-23 March 2021, an online survey was conducted targeting the enrolled students of university in Chungcheongnam-do. Out of a total of 398 questionnaires, a total of 394 questionnaires (98.9%) were used for the final analysis after excluding four questionnaires with low reliability. This study was analyzed using SPSS by IBM 23.0(New York, NY, USA) and AMOS 21.0 (New York, NY, USA). In the results of this study, first, the factors such as imported goods/famous brands, high-priced articles, and status symbol except for pursuit of trend of conspicuous consumption tendency had significant effects on the self-expression satisfaction. Second, the factors such as imported goods/famous brands, high-priced articles, and status symbol except for pursuit of trend of conspicuous consumption tendency of Gen Z through SNS activities had significant effects on the SNS use satisfaction. Third, the self-expression satisfaction of Gen Z through SNS had significant effects on the SNS use satisfaction.