Anti-inflammatory Quinoline Alkaloids from the Roots of Waltheria indica.

Sixteen new quinoline alkaloids (1a-7, 8a, 9, 10, 13-15, 17, and 21) and 10 known analogs (8b, 11, 12, 16, 18-20, and 22-24), along with three known cyclopeptide alkaloids (25-27), were isolated from the roots of Waltheria indica. The structures of the new compounds were elucidated by detailed NMR and circular dichroism with computational support and mass spectrometry data interpretation. Anti-inflammatory potential of isolates was evaluated based on inhibition of lipopolysaccharide (LPS)-induced nitric oxide (NO) production and tumor necrosis factor-alpha (TNF-α)-induced nuclear factor kappa B (NF-κB) activity with cell culture models. In the absence of cell growth inhibition, compounds 6, 8a, 9-11, 13, 21, and 24 reduced TNF-α-induced NF-κB activity with IC50 values ranging from 7.1 to 12.1 µM, comparable to the positive control (BAY 11-7082, IC50 = 9.7 µM). Compounds 6, 8a, 8b, and 11 showed significant NO-inhibitory activity with IC50 values ranging from 11.0 to 12.8 µM, being more active than the positive control (l-NMMA, IC50 = 22.7 µM). Structure-activity relationships indicated that NO inhibitory activity was significantly affected by C-8 substitution. Inhibition of LPS-induced nitric oxide synthase (iNOS) by 8b [(5S)-waltherione M, IC50 11.7 ± 0.8 µM] correlated with inhibition of iNOS mRNA expression. The biological potential of W. indica metabolites supports the traditional use of this plant for the treatment of inflammatory-related disorders.

Coumarinolignans with Reactive Oxygen Species (ROS) and NF-κB Inhibitory Activities from the Roots of Waltheria indica.

Seven new coumarinolignans, walthindicins A-F (1a, 1b, 2-5, 7), along with five known analogs (6, 8-11), were isolated from the roots of Waltheria indica. The structures of the new compounds are determined by detailed nuclear magnetic resonance (NMR), circular dichroism (CD) with extensive computational support, and mass spectroscopic data interpretation. Compounds were tested for their antioxidant activity in Human Cervical Cancer cells (HeLa cells). Compounds 1a and 6 showed higher reactive oxygen species (ROS) inhibitory activity at 20 µg/mL when compared with other natural compound-based antioxidants such as ascorbic acid. Considering the role of ROS in nuclear-factor kappa B (NF-κB) activation, compounds 1a and 6 were evaluated for NF-κB inhibitory activity and showed a concentration-dependent inhibition in Human Embryonic Kidney 293 cells (Luc-HEK-293).

The Wisdom of Indigenous Healers.

The wisdom of indigenous peoples is manifest in ways of knowing, seeing, and thinking that are passed down orally from generation to generation. This article takes the reader on a journey through three distinct ways of knowing, specifically as they relate to healing and health. The authors are a Midewanniquay, or Water Woman, of the Ojibway-Anishinabe people of the upper Midwest in the United States and Canada; a lomilomi healer from Hawaii; and an initiated Priest in the Yoruba tradition of West Africa. The philosophies of all three cultures emphasize the importance of spirituality to health and well-being (or healing process), but each has unique ways in which it nurtures relationship with the Creator, the earth, and humankind through sacred rituals and healing practices.

The wisdom of indigenous healers.

The wisdom of indigenous peoples is manifest in ways of knowing, seeing, and thinking that are passed down orally from generation to generation. This article takes the reader on a journey through three distinct ways of knowing, specifically as they relate to healing and health. The authors are a Midewanniquay, or Water Woman, of the Ojibway-Anishinabe people of the upper Midwest in the United States and Canada; a Iomilomi healer from Hawaii; and an initiated Priest in the Yoruba tradition of West Africa. The philosophies of all three cultures emphasize the importance of spirituality to health and wellbeing (or healing process), but each has unique ways in which it nurtures relationship with the Creator, the earth, and humankind through sacred rituals and healing practices.