RESUMO
The decoction of the stem barks from Bowdichia virgilioides KUNTH is a folk remedy used to treat inflammatory disorders in Latin American and Brazil. In the present study, the wound healing activity of aqueous extract of the stem bark from B. virgilioides, called AEBv, was evaluated by the rate of healing by wound contraction and period of epithelization at different days post-wound using the wound excisional model. On day 9, the AEBv-treated animals exhibited significative reduction in the wound area when compared with controls. In wound infected with S. aureus, the AEBv significantly improved the wound contraction when compared to the saline-treated mice. The histological analysis showed that AEBv induced a collagen deposition, increase in the fibroblast count and few inflammatory cells than compared to saline-treated group. The expression of collagen type I was increased in the group treated with AEBv as indicated by immunohistochemical staining. In vitro, the AEBv was effective only against S. aureus but not against P. aeruginosa. Together, the results of this study demonstrate, for the first time, the healing and antimicrobiological effects of aqueous extract of the stem bark from B. virgilioides in the therapy of skin wounds.
Assuntos
Antibacterianos/farmacologia , Fabaceae/química , Casca de Planta/química , Extratos Vegetais/farmacologia , Cicatrização/efeitos dos fármacos , Animais , Modelos Animais de Doenças , Feminino , Tecido de Granulação/efeitos dos fármacos , Imuno-Histoquímica , Masculino , Camundongos , Staphylococcus aureus/efeitos dos fármacos , Staphylococcus aureus/isolamento & purificaçãoRESUMO
We describe a case of wound infection by multidrug-resistant Staphylococcus sciuri in a patient admitted to hospital for injuries in Agreste Alagoas, Brazil, identified through broad-spectrum PCR and sequencing of 16S rDNA gene. Due to its high resistance profile, the infection was characterized as methicillin-resistant Staphylococcus presenting sensitive only to vancomycin and chloramphenicol. The injury resulting from trauma associated with infection resulted in amputation of the infected limb.
Assuntos
Farmacorresistência Bacteriana/genética , Resistência a Múltiplos Medicamentos/genética , Staphylococcus aureus Resistente à Meticilina/isolamento & purificação , Infecções Estafilocócicas/microbiologia , Ferimentos e Lesões/microbiologia , Adulto , Amputação Cirúrgica , Antibacterianos/farmacologia , Cloranfenicol/farmacologia , Humanos , Masculino , Staphylococcus aureus Resistente à Meticilina/efeitos dos fármacos , Staphylococcus aureus Resistente à Meticilina/genética , RNA Ribossômico 16S/genética , Infecções Estafilocócicas/tratamento farmacológico , Infecções Estafilocócicas/cirurgia , Vancomicina/farmacologia , Ferimentos e Lesões/cirurgiaRESUMO
Dental health care providers and patients are exposed during ongoing work to contamination by the water used in the dental units, due to accidental swallowing or aspiration of the sprays generated by the high-speed handpiece and the threeway syringe. This study evaluated the quality of water in dental units in the public dental care system of Maceio, Alagoas, Brazil, by conducting analyses of contamination by total coliforms, E.Coli, heterotrophic bacteria and filamentous fungi. We collected 200 mL of water at 5 sites in 6 dental offices of the Department of Health located in different parts of the city. A total 212 isolates and 16 genera of filamentous fungi were identified in the water collected from the dental units. Total coliforms indicated that the water used in dental units was not appropriate for human consumption. The high levels of contamination found in this study showed that water was a potential source of cross-infection.
Assuntos
Equipamentos Odontológicos/microbiologia , Consórcios Microbianos , Microbiologia da Água , Acremonium/isolamento & purificação , Aspergillus/isolamento & purificação , Carga Bacteriana , Contagem de Colônia Microbiana , Equipamentos Odontológicos de Alta Rotação/microbiologia , Enterobacteriaceae/isolamento & purificação , Contaminação de Equipamentos , Escherichia coli/isolamento & purificação , Exophiala/isolamento & purificação , Fungos/isolamento & purificação , Humanos , Penicillium/isolamento & purificação , Seringas/microbiologia , Qualidade da Água , Abastecimento de ÁguaRESUMO
Dental health care providers and patients are exposed duringongoing work to contamination by the water used in the dentalunits, due to accidental swallowing or aspiration of thesprays generated by the high-speed handpiece and the threewaysyringe. This study evaluated the quality of water indental units in the public dental care system of Maceió,Alagoas, Brazil, by conducting analyses of contamination bytotal coliforms, E.Coli, heterotrophic bacteria and filamentousfungi. We collected 200 mL of water at 5 sites in 6 dentaloffices of the Department of Health located in different partsof the city. A total 212 isolates and 16 genera of filamentousfungi were identified in the water collected from the dentalunits. Total coliforms indicated that the water used in dentalunits was not appropriate for human consumption. The highlevels of contamination found in this study showed that waterwas a potential source of cross-infection...
Durante a rotina de trabalho, a equipe odontológica e ospacientes ficam expostos a possível contaminação pela água utilizadanas unidades dentais, devido à ingestão acidental da águaou pela aspiração dos sprays gerados pela caneta de alta rotaçãoe seringa tríplice. Este estudo avaliou a qualidade da água usadaem consultórios odontológicos da rede pública estadual deMaceió, Alagoas, Brasil, através da análise de contaminação porcoliformes totais e E.coli, bactérias heterotróficas e fungos filamentosos.Foram coletados 200 mL de água, em cinco pontos deseis consultórios odontológicos pertencentes à Secretaria Estadualde Saúde, localizados em diferentes bairros da cidade. Umtotal de 212 isolados e 16 gêneros de fungos filamentosos foramidentificados. A presença de coliformes totais indicou que a águautilizada nas unidades dentais era imprópria para consumohumano. O alto índice de contaminação mostrou que as águasestudadas eram uma fonte potencial de infecções cruzadas...