RESUMO
Soil salinity is one of the major limiting factors for poor crop yield in the world. Increasing salinity in the soil is a challenge for agriculture. In the recent past, plant growth-promoting rhizobacteria (PGPR) are being used to enhance plant growth in various conditions. However, the saline-tolerant PGPR are of great use for plant growth under saline condition. In the present study, saline-tolerant E. hormaechei (MF957335) was isolated from saline water. E. hormaechei (MF957335) was tested for its potassium and calcium solubilizing efficiency using Scanning Electron Microscopy-Energy Dispersive X-Ray (SEM-EDX). E. hormaechei (MF957335) and K-Feldspar treatments significantly increased plant growth as compared to untreated plants (negative control). E. hormaechei (MF957335) significantly increased fresh biomass, shoot and root length of tomato plants. Among all the NaCl treatments, maximum fruits (9.66) were achieved in 250 mM NaCl + E. hormaechei treatment. Similar results with increased fruit numbers were obtained in K-Feldspar-treated plants. Apart from the plant growth, fresh biomass and fruit numbers, tomatoes from K-Feldspar-treated plants were large, fleshy and deep red colored. The study could demonstrate bioavailability of potassium from K-feldspar for tomato cultivation. Control plants tomato were small, non-fleshy, yellowish red, and infected with calcium deficiency disease blossom-end rot. The present study demonstrates the role of E. hormaechei (MF957335) in plant growth, yield promotion and disease tolerance by potassium and calcium solubilization, respectively. The study showed that E. hormaechei (MF957335) could be applied to saline and non-saline soils to enhance tomato yield.
Assuntos
Cálcio/metabolismo , Enterobacter/metabolismo , Potássio/metabolismo , Solanum lycopersicum/crescimento & desenvolvimento , Solanum lycopersicum/microbiologia , Enterobacter/isolamento & purificação , Desenvolvimento Vegetal/fisiologia , Raízes de Plantas/microbiologia , Águas Salinas , Salinidade , Tolerância ao Sal , Cloreto de Sódio , Solo/química , Microbiologia do SoloRESUMO
Humans have been using natural resources for their daily life support. The population boom around the world has created a need to develop a new technique for increased crop production. Chemical fertilizers used in modern agriculture lead to pollution, besides increase in farming costs. To solve this problem, the present study deals with a natural halotolerant Enterobacter hormaechei which can fix N and solubilize desired macronutrients P and K. Enterobacter hormaechei was able to solubilize K-feldspar into potassium (97.5 ± 1.76 mg/L), tri-calcium phosphate into phosphate (99.7 ± 02 µg/mL) and it also produced IAA (47.87 ± 0.85 mg/L). Experiments, including morphological and chemical analysis, have provided a new growth pattern in Lycopersicum esculentum. Tomato seeds (Lycopersicum esculentum) treated with Enterobacter hormaechei enhanced biomass and an increase in shoot length when compared to control. It enhanced not only plant growth but modified the root architecture leading to improved crop productivity.
Assuntos
Desenvolvimento Vegetal , Solanum lycopersicum , Enterobacter/genética , Fertilizantes , Humanos , Raízes de PlantasRESUMO
BACKGROUND: Urinary tract infections (UTI) are the most common of infections after renal transplantation. The consequences of UTIs in this population are serious, with increased morbidity and hospitalisation rates as well as acute allograft dysfunction. UTIs may impair overall graft and patient survival. We aimed to identify the prevalence and risk factors for post-transplant UTIs and assess UTIs' effect on renal function during a UTI episode and if they result in declining allograft function at 2 years post-transplant. Additionally, the causative organism, the class of antibacterial drug employed for each UTI episode and utilisation rates of trimethoprim/sulfamethoxazole (TMP/SMX) prophylaxis were also quantified. METHODS: This was a retrospective study of 72 renal transplant patients over a 5-year period who were managed at the Royal Brisbane and Women's Hospital. Patient charts, pathology records and dispensing histories were reviewed as part of this study and all UTIs from 2 years post transplantation were captured. RESULTS: Of these patients, 20 (27.8%) had at least one UTI. Older age (p = 0.015), female gender (p < 0.001), hyperglycaemia (p = 0.037) and acute rejection episodes (p = 0.046) were risk factors for developing a UTI on unadjusted analysis. Female gender (OR 4.93) and age (OR 1.03) were statistically significant risk factors for a UTI on adjusted analysis. On average, there was a 14.4% (SEM 5.20) increase in serum creatinine during a UTI episode, which was statistically significant (p = 0.027), and a 9.1% (SEM 6.23) reduction in serum creatinine after the UTI episode trending toward statistical significance. (p = 0.076). Common organisms (Escherichia coli and Klebsiella pneumoniae) accounted for 82% of UTI episodes with 70% of UTI cases requiring only a single course of antibiotic treatment. Furthermore, the antibiotic class used was either a penicillin (49%) or cephalosporin (36%) in the majority of UTIs. The use of TMP/SMX prophylaxis for Pneumocystis carinii pneumonia prophylaxis did not influence the rate of UTI, with > 90% of the cohort using this treatment. CONCLUSIONS: There was no significant change in serum creatinine and estimated glomerular filtrate rate from baseline to 2 years post-transplant between those with and without a UTI.
Assuntos
Hospitais de Ensino/tendências , Transplante de Rim/efeitos adversos , Transplantados , Infecções Urinárias/diagnóstico , Infecções Urinárias/epidemiologia , Adulto , Infecções por Escherichia coli/diagnóstico , Infecções por Escherichia coli/epidemiologia , Feminino , Humanos , Transplante de Rim/tendências , Infecções por Klebsiella/diagnóstico , Infecções por Klebsiella/epidemiologia , Masculino , Pessoa de Meia-Idade , Infecções por Pneumocystis/diagnóstico , Infecções por Pneumocystis/epidemiologia , Queensland/epidemiologia , Estudos RetrospectivosRESUMO
The use of water purifiers is intensively catching up and disposing of reverse osmosis reject water is of great concern. Reject water management using conventional methods is costly and harmful to the environment. To address this issue, the present study aims to utilize reverse osmosis reject wastewater using an eco-friendly approach. Juncus rigidus was treated with reject wastewater containing different salinity levels. Wastewater-treated plant dry biomass increased with increasing reject water salinity, and 625.3 g dry biomass recovered in treatment-B (~18,520 ppm). However, ~23,220 ppm wastewater salinity was lethal to the plants. The cellulose was extracted by alkali hydrolysis. The cellulose content in the wastewater-treated biomass was significantly higher in Treatment-B compared to both the control and Treatment-A (~12,744 ppm). The water salinity enhanced the cellulose (26.49 %) production in J. rigidus. Cellulose purity was confirmed using spectroscopic and thermogravimetric means. XRD shows highest crystallinity Index (77.29) with a d-spacing of 4.7 Å and 5.7 nm crystallite size in treatment-B. FTIR results reveal well-defined relevant peaks for OH, CH, CO, CH2, C-O-C, CO groups in treatment-B cellulose. Salinity impacts carboxyl groups in treatment B cellulose with a sharper and intense peak at 1644 cm-1 responsible for water absorption. Treatment-B exhibits higher thermal stability due to increased crystallinity. DSC shows endothermic depolymerization of cellulose with distinct peaks for different treatments. Morphological traits got better with increasing salinity with no adverse effect on cellulose. Salinity moderately affected the water absorption capacity of cellulose. All cellulose samples were devoid of gram-negative bacteria known by microbial test. This pioneering work underscores the plant's remarkable capacity not only to accomplish the circular economy by the valorization of wastewater obtained from various water purifiers for Juncus cultivation for cellulose production for diverse applications but also to generate income from wastewater.
Assuntos
Biomassa , Celulose , Salinidade , Eliminação de Resíduos Líquidos , Águas Residuárias , Celulose/química , Águas Residuárias/química , Eliminação de Resíduos Líquidos/métodos , Estresse SalinoRESUMO
An expedient and efficient approach is used to synthesize a new class of metallo-polymeric microspheres (MPMs) as antimicrobials to succumb the wide range of bacteria from water. Three types of MPMs, that is, poly[Silver (I)-methacrylate-co-methylmethacrylate] (pAgMA), poly[Copper (II)-methacrylate-co-methyl methacrylate] (pCuMA), and poly[Nickel (II)-methacrylate-co-methylmethacrylate] (pNiMA), are prepared via radical suspension polymerization technique in 3D shape with porous texture. The structural and morphological characterization of the prepared microspheres are examined by analytical techniques. The antimicrobial potentialities of prepared MPMs are investigated at the laboratory scale study, revealing that the MPMs exhibit strong antibacterial activity (≈99.9% killing) against Gram-negative and Gram-positive bacteria [Enterobacter hormaechei (EH), Bacillus megatarium (BM), and Bacillus bataviensis (BB)]. The MacConkey agar medium test reveals that MPMs have substantial biocidal efficacy against broad-spectrum Gram-negative bacteria present in tap water. The MPMs exhibit significant antimicrobial efficacy via contact killing owe to the presence of integrated biocidal metal moiety, which represents that the MPMs are safe for water disinfection.
Assuntos
Microesferas , Porosidade , Desinfecção/métodos , Testes de Sensibilidade Microbiana , Antibacterianos/farmacologia , Antibacterianos/química , Anti-Infecciosos/farmacologia , Anti-Infecciosos/química , Purificação da Água/métodos , Polímeros/química , Polímeros/farmacologiaRESUMO
BACKGROUND: Individuals can experience difficulties pursuing their goals amid multiple competing priorities in their environment. Effective goal dynamics require flexible and generalizable pursuit skills. Supporting successful goal pursuit requires a perpetually adapting intervention responsive to internal states. OBJECTIVE: The purpose of this study was to (1) develop a flexible intervention that can adapt to an individual's changing short to medium-term goals and be applied to their daily life and (2) examine the feasibility and acceptability of the just-in-time adaptive intervention for goal pursuit. METHODS: This study involved 3 iterations to test and systematically enhance all aspects of the intervention. During the pilot phase, 73 participants engaged in an ecological momentary assessment (EMA) over 1 month. After week 1, they attended an intervention training session and received just-in-time intervention prompts during the following 3 weeks. The training employed the Capability, Opportunity, Motivation, and Behavior (COM-B) framework for goal setting, along with mental contrasting with implementation intentions (MCII). Subsequent prompts, triggered by variability in goal pursuit, guided the participants to engage in MCII in relation to their current goal. We evaluated feasibility and acceptability, efficacy, and individual change processes by combining intensive (single-case experimental design) and extensive methods. RESULTS: The results suggest that the digital intervention was feasible and acceptable to participants. Compliance with the intervention was high (n=63, 86%). The participants endorsed high acceptability ratings relating to both the study procedures and the intervention. All participants (N=73, 100%) demonstrated significant improvements in goal pursuit with an average difference of 0.495 units in the outcome (P<.001). The results of the dynamic network modeling suggest that self-monitoring behavior (EMA) and implementing the MCII strategy may aid in goal reprioritization, where goal pursuit itself is a driver of further goal pursuit. CONCLUSIONS: This pilot study demonstrated the feasibility and acceptability of a just-in-time adaptive intervention among a nonclinical adult sample. This intervention used self-monitoring of behavior, the COM-B framework, and MCII strategies to improve dynamic goal pursuit. It was delivered via an Ecological Momentary Intervention (EMI) procedure. Future research should consider the utility of this approach as an additional intervention element within psychological interventions to improve goal pursuit. Sustaining goal pursuit throughout interventions is central to their effectiveness and warrants further evaluation.
RESUMO
Background: Many technological interventions designed to promote physical activity (PA) have limited efficacy and appear to lack important factors that could increase engagement. This may be due to a discrepancy between research conducted in this space, and software designers' and developers' use of this research to inform new digital applications. Objectives: This study aimed to identify (1) what are the variables that act as barriers and facilitators to PA and (2) which PA variables are currently considered in the design of technologies promoting PA including psychological, physical, and personal/contextual ones which are critical in promoting PA. We emphasize psychological variables in this work because of their sparse and often simplistic integration in digital applications for PA. Methods: We conducted two systematized reviews on PA variables, using PsycInfo and Association for Computing Machinery Digital Libraries for objectives 1 and 2. Results: We identified 38 PA variables (mostly psychological ones) including barriers/facilitators in the literature. 17 of those variables were considered when developing digital applications for PA. Only few studies evaluate PA levels in relation to these variables. The same barriers are reported for all weight groups, though some barriers are stronger in people with obesity. Conclusions: We identify PA variables and illustrate the lack of consideration of these in the design of PA technologies. Digital applications to promote PA may have limited efficacy if they do not address variables acting as facilitators or barriers to participation in PA, and that are important to people representing a range of body weight characteristics.
RESUMO
The effects of music on bodily movement and feelings, such as when people are dancing or engaged in physical activity, are well-documented-people may move in response to the sound cues, feel powerful, less tired. How sounds and bodily movements relate to create such effects? Here we deconstruct the problem and investigate how different auditory features affect people's body-representation and feelings even when paired with the same movement. In three experiments, participants executed a simple arm raise synchronised with changing pitch in simple tones (Experiment 1), rich musical sounds (Experiment 2) and within different frequency ranges (Experiment 3), while we recorded indirect and direct measures on their movement, body-representations and feelings. Changes in pitch influenced people's general emotional state as well as the various bodily dimensions investigated-movement, proprioceptive awareness and feelings about one's body and movement. Adding harmonic content amplified the differences between ascending and descending sounds, while shifting the absolute frequency range had a general effect on movement amplitude, bodily feelings and emotional state. These results provide new insights in the role of auditory and musical features in dance and exercise, and have implications for the design of sound-based applications supporting movement expression, physical activity, or rehabilitation.
Assuntos
Movimento/fisiologia , Música , Percepção da Altura Sonora/fisiologia , Localização de Som/fisiologia , Adulto , Feminino , Humanos , MasculinoRESUMO
OBJECTIVES: Psychologically informed practice (PIP) is advocated for physiotherapists to help people with chronic pain. There is little research observing how PIP is delivered in clinical practice. This study describes behaviours and techniques used by experienced physiotherapists working with groups of people with chronic pain. SETTING AND PARTICIPANTS: Experienced physiotherapists (n=4) were observed working with groups of people with chronic pain in out-patient pain management, and physiotherapy departments, in a large UK city centre teaching hospital. DESIGN: We observed the clinical behaviours and interpersonal skills of experienced psychologically informed physiotherapists, enriched by their accounts of intentions. The physiotherapists were audio and video recorded delivering group movement sessions. Recordings were reviewed with the physiotherapists for elaboration of intentions, then thematically analysed for comparison with defined CBT competencies. RESULTS: Four themes representing physiotherapist intentions when working with people with chronic pain were identified; building a therapeutic alliance, reducing perceived threat, reconceptualising beliefs and somatic experience, and fostering self-efficacy. The physiotherapists also reflected on challenges including engaging patients in self-management, encouraging activity and reinforcing rather than correcting movement. Considerable overlap existed between the observed behaviours in this study and existing CBT competencies. CONCLUSIONS: This paper complements current recommendations for delivering psychologically informed physiotherapy by providing examples of these skills being used in clinical practice. Further research supporting the development of training for, and mentoring of, physiotherapists, to promote competence and confidence in delivering psychologically informed interventions is recommended.
Assuntos
Dor Crônica/psicologia , Dor Crônica/terapia , Terapia Cognitivo-Comportamental , Conhecimentos, Atitudes e Prática em Saúde , Manejo da Dor/métodos , Fisioterapeutas , Modalidades de Fisioterapia , Feminino , Humanos , Masculino , Pesquisa QualitativaRESUMO
Immunoglobulin A nephropathy (IgAN) is the most commonly diagnosed glomerulonephritis worldwide. It is usually idiopathic and may be associated with many other diseases. Recently, biological agents including tumour necrosis factor alpha (TNFα) inhibitors have been identified as a potential cause for IgAN. We report the case of a 39-year-old woman who presented with renal dysfunction and visible haematuria. She had a background of Crohn's disease (CD) and had been on adalimumab for 4 years following a right hemicolectomy. Subsequently, she underwent a renal biopsy that demonstrated IgAN and adalimumab was ceased. Following a flare in her CD, she was commenced on infliximab, which led to remission of the IgAN and CD. This is the first case to demonstrate the occurrence of IgAN as a complication of a TNFα inhibitor (adalimumab) that remained in remission despite the commencement of a second TNFα inhibitor (infliximab).
Assuntos
Adalimumab/efeitos adversos , Doença de Crohn/cirurgia , Fármacos Gastrointestinais/uso terapêutico , Glomerulonefrite por IGA/induzido quimicamente , Infliximab/uso terapêutico , Fator de Necrose Tumoral alfa/antagonistas & inibidores , Adalimumab/uso terapêutico , Adulto , Biópsia , Colectomia , Doença de Crohn/tratamento farmacológico , Doença de Crohn/fisiopatologia , Feminino , Glomerulonefrite por IGA/patologia , Humanos , Testes de Função Renal , Resultado do TratamentoRESUMO
Research and development for interactive digital health interventions requires multi-disciplinary expertise in identifying user needs, and developing and evaluating each intervention. Two of the central areas of expertise required are Health (broadly defined) and Human-Computer Interaction. Although these share some research methods and values, they traditionally have deep differences that can catch people unawares, and make interdisciplinary collaborations challenging, resulting in sub-optimal project outcomes. The most widely discussed is the contrast between formative evaluation (emphasised in Human-Computer Interaction) and summative evaluation (emphasised in Health research). However, the differences extend well beyond this, from the nature of accepted evidence to the culture of reporting. In this paper, we present and discuss seven lessons that we have learned about the contrasting cultures, values, assumptions and practices of Health and Human-Computer Interaction. The lessons are structured according to a research lifecycle, from establishing the state of the art for a given digital intervention, moving through the various (iterative) stages of development, evaluation and deployment, through to reporting research results. Although our focus is on enabling people from different disciplinary backgrounds to work together with better mutual understanding, we also highlight ways in which future research in this interdisciplinary space could be better supported.
RESUMO
Pain-related emotions are a major barrier to effective self rehabilitation in chronic pain. Automated coaching systems capable of detecting these emotions are a potential solution. This paper lays the foundation for the development of such systems by making three contributions. First, through literature reviews, an overview of how pain is expressed in chronic pain and the motivation for detecting it in physical rehabilitation is provided. Second, a fully labelled multimodal dataset (named 'EmoPain') containing high resolution multiple-view face videos, head mounted and room audio signals, full body 3D motion capture and electromyographic signals from back muscles is supplied. Natural unconstrained pain related facial expressions and body movement behaviours were elicited from people with chronic pain carrying out physical exercises. Both instructed and non-instructed exercises were considered to reflect traditional scenarios of physiotherapist directed therapy and home-based self-directed therapy. Two sets of labels were assigned: level of pain from facial expressions annotated by eight raters and the occurrence of six pain-related body behaviours segmented by four experts. Third, through exploratory experiments grounded in the data, the factors and challenges in the automated recognition of such expressions and behaviour are described, the paper concludes by discussing potential avenues in the context of these findings also highlighting differences for the two exercise scenarios addressed.