RESUMO
New analogs of the well-known bioactive trihydroxy-stilbene resveratrol were synthesized to investigate their potential biological activity. The focus was on assessing their ability to inhibit cholinesterase enzymes (ChEs) and their antioxidative properties, which were thoroughly examined. New resveratrol analogs were synthesized through Wittig or McMurry reaction in moderate-to-good yields. In all synthetic pathways, mixtures of cis- and trans-isomers were obtained, then separated by chromatography, and trans-isomers were isolated as targeted structures. The stilbene derivatives underwent evaluation for antioxidant activity (AOA) using DPPH and CUPRAC assay, and their potential to inhibit acetylcholinesterase (AChE) and butyrylcholinesterase (BChE) was also measured. The biological tests have shown that the same compounds exhibited significant antioxidative and butyrylcholinesterase inhibitory potential, as evidenced by lower IC50 values compared to the established standards, trans-resveratrol, and galantamine, respectively. Additionally, molecular docking of the selected synthesized potential inhibitors to the enzyme's active site was performed, followed by assessing the complex stability using molecular dynamics simulation lasting 100 ns. Lastly, the new compounds underwent examination to determine their potential mutagenicity.
Assuntos
Acetilcolinesterase , Butirilcolinesterase , Butirilcolinesterase/metabolismo , Acetilcolinesterase/metabolismo , Inibidores da Colinesterase/química , Resveratrol/farmacologia , Simulação de Acoplamento Molecular , Relação Estrutura-Atividade , Antioxidantes/farmacologiaRESUMO
The targeted compounds in this research, resveratrol analogs 1-14, were synthesized as mixtures of isomers by the Wittig reaction using heterocyclic triphenylphosphonium salts and various benzaldehydes. The planned compounds were those possessing the trans-configuration as the biologically active trans-resveratrol. The pure isomers were obtained by repeated column chromatography in various isolated yields depending on the heteroaromatic ring. It was found that butyrylcholinesterase (BChE) was more sensitive to the heteroaromatic resveratrol analogs than acetylcholinesterase (AChE), except for 6, the methylated thiophene derivative with chlorine, which showed equal inhibition toward both enzymes. Compounds 5 and 8 achieved the highest BChE inhibition with IC50 values of 22.9 and 24.8 µM, respectively. The same as with AChE and BChE, methylated thiophene subunits of resveratrol analogs showed better enzyme inhibition than unmethylated ones. Two antioxidant spectrophotometric methods, DPPH and CUPRAC, were applied to determine the antioxidant potential of new heteroaromatic resveratrol analogs. The molecular docking of these compounds was conducted to visualize the ligand-active site complexes' structure and identify the non-covalent interactions responsible for the complex's stability, which influence the inhibitory potential. As ADME properties are crucial in developing drug product formulations, they have also been addressed in this work. The potential genotoxicity is evaluated by in silico studies for all compounds synthesized.
Assuntos
Antioxidantes , Butirilcolinesterase , Inibidores da Colinesterase , Simulação de Acoplamento Molecular , Resveratrol , Resveratrol/análogos & derivados , Resveratrol/química , Resveratrol/farmacologia , Resveratrol/síntese química , Inibidores da Colinesterase/farmacologia , Inibidores da Colinesterase/química , Inibidores da Colinesterase/síntese química , Antioxidantes/química , Antioxidantes/farmacologia , Antioxidantes/síntese química , Butirilcolinesterase/metabolismo , Butirilcolinesterase/química , Acetilcolinesterase/metabolismo , Acetilcolinesterase/química , Humanos , Relação Estrutura-AtividadeRESUMO
Triazoles and triazolium salts are very common subunits in the structures of various drugs. Medicaments with a characteristic 1,2,3-triazole core are also being developed to treat neurodegenerative disorders associated with cholinesterase enzyme activity. Several naphtho- and thienobenzo-triazoles from our previous research emerged as being particularly promising in that sense. For this reason, in this research, new naphtho- and thienobenzo-triazoles 23-34, as well as 1,2,3-triazolium salts 44-51, were synthesized and tested. Triazolium salts 44-46 showed excellent activity while salts 47 and 49 showed very good inhibition toward both butyrylcholinesterase (BChE) and acetylcholinesterase (AChE) enzymes. In contrast, neutral photoproducts were shown to be selective towards BChE but with very good inhibition potential as molecules 24-27. The representative of newly prepared compounds, 45 and 50, were stable in aqueous solution and revealed intriguing fluorimetric properties, characterized by a strong Stokes shift of >160 nm. Despite their condensed polycyclic structure shaped similarly to well-known DNA-intercalator ethidium bromide, the studied compounds did not show any interaction with ds-DNA, likely due to the unfavorable steric hindrance of substituents. However, the studied dyes bind proteins, particularly showing very diverse inhibition properties toward AChE and BChE. In contrast, neutral photoproducts were shown to be selective towards a certain enzyme but with moderate inhibition potential. The molecular docking of the best-performing candidates to cholinesterases' active sites identified cation-π interactions as the most responsible for the stability of the enzyme-ligand complexes. As genotoxicity studies are crucial when developing new active substances and finished drug forms, in silico studies for all the compounds synthesized have been performed.
Assuntos
Butirilcolinesterase , Inibidores da Colinesterase , Inibidores da Colinesterase/farmacologia , Acetilcolinesterase , Simulação de Acoplamento Molecular , Sais , Complexos Multienzimáticos , Triazóis/farmacologiaRESUMO
3-Hydroxymethyl-2-aminonaphthalene photocage (photoremovable protecting group) 2 was synthesized and transformed to different ethers and esters to investigate the applicability to decage alcohols and carboxylic acids, respectively. The photoelimination of carboxylic acids takes place relatively efficiently (ΦR = 0.11) upon excitation with near-visible light, contrary to the elimination of alcohols. The scope of the decaging of both alcohols and esters was demonstrated on several examples, including aliphatic and aromatic substrates, carbohydrates, and nonsteroidal anti-inflammatory drugs. The photophysical properties of the photocage and its models, methyl ether 4a and acetyl ester 5a, were investigated. The fluorescence quantum yields (Φf = 0.40-0.002) were found to be reversely proportional to the efficiency of elimination of OH, alcohols, or carboxylic acids. The decaging photochemical reaction mechanism was investigated experimentally by transient absorption techniques with time scales from femtoseconds to seconds and computationally on the TD-DFT level of theory. The photoelimination of carboxylates takes place directly in the singlet excited state by a homolytic cleavage producing a radical pair within 1 ns. The subsequent electron transfer gives rise to aminonaphthalene carbocation and the carboxylate. A wide scope of substrates that can be decaged relatively efficiently with near-visible light and the chromo-orthogonal compatibility of aminonaphthalene and aniline derivatives render these photocages potentially applicable in organic synthesis or biology.
RESUMO
In this investigation, the excited-state evolution in a series of all-trans stilbenoid compounds, displaying a low-lying dark singlet state of 2Ag-like symmetry nearly degenerate with the bright 1Bu state, was unveiled by employing advanced ultrafast spectroscopies while probing the effect of solvent polarizability. Together with the dual emission, femtosecond transient absorption and broadband fluorescence up-conversion disclosed the double nature of the 2Ag-like state showing both singlet features, a lifetime typical of a singlet and the ability to emit, and a triplet character, exhibiting a triplet-like absorption spectrum. The ultrafast formation (in hundreds of femtoseconds) from the non-relaxed upper singlet state led to the identification of 2Ag as the correlated triplet pair of singlet fission. The spectral difference obtained by comparison of transient absorption peaks of the 2Ag (1TT) and the triplet states was found to be in remarkable agreement with the observed triplet yield and the 1(TT) separation rate constant. Indeed, this spectral shift provided an experimental method to gain qualitative insight into the ease of separation of the 1(TT) and the relative SF efficiency. The highly conjugated polyene-like structures enable the ultrafast formation of the double triplet, but then the large binding energy prevents the triplet separation and thus the effective completion of singlet fission. Even though thermodynamically feasible for all the investigated stilbenoids according to TD-DFT calculations, singlet fission resulted to occur efficiently in the case of 1-(pyridyl-4-ylethenyl)-4-(p-nitrostyryl)benzene and nitro-styrylfuran with the triplet yield reaching 120% and 140%, respectively, triggered by their greatly enhanced intramolecular charge transfer character relative to the other compounds in the series.
RESUMO
This study aims to test the inhibition potency of new thienobenzo/naphtho-triazoles toward cholinesterases, evaluate their inhibition selectivity, and interpret the obtained results by molecular modeling. The synthesis of 19 new thienobenzo/naphtho-triazoles by two different approaches resulted in a large group of molecules with different functionalities in the structure. As predicted, most prepared molecules show better inhibition of the enzyme butyrylcholinesterase (BChE), considering that the new molecules were designed according to the previous results. Interestingly, the binding affinity of BChE for even seven new compounds (1, 3, 4, 5, 6, 9, and 13) was similar to that reported for common cholinesterase inhibitors. According to computational study, the active thienobenzo- and naphtho-triazoles are accommodated by cholinesterases through H-bonds involving one of the triazole's nitrogens, π-π stacking between the aromatic moieties of the ligand and aromatic residues of the active sites of cholinesterases, as well as π-alkyl interactions. For the future design of cholinesterase inhibitors and search for therapeutics for neurological disorders, compounds with a thienobenzo/naphtho-triazole skeleton should be considered.
Assuntos
Butirilcolinesterase , Inibidores da Colinesterase , Inibidores da Colinesterase/química , Butirilcolinesterase/metabolismo , Triazóis/farmacologia , Triazóis/química , Relação Estrutura-Atividade , Acetilcolinesterase/metabolismo , Simulação de Acoplamento MolecularRESUMO
New 1,2,3-triazolo(thieno)stilbenes were synthesized as mixtures of isomers and efficiently photochemically transformed to their corresponding substituted thienobenzo/naphtho-triazoles in high isolated yields. The resulting photoproducts were studied as acetyl- (AChE) and butyrylcholinesterase (BChE) inhibitors without or with interconnected inhibition potential of TNF-α cytokine production. The most promising anti-inflammatory activity was shown again by naphtho-triazoles, with a derivative featuring 4-pentenyl substituents exhibiting notable potential as a cholinesterase inhibitor. To identify interactions between ligands and the active site of cholinesterases, molecular docking was performed for the best potential inhibitors. Additionally, molecular dynamics simulations were employed to assess and validate the stability and flexibility of the protein-ligand complexes generated through docking.
Assuntos
Acetilcolinesterase , Butirilcolinesterase , Butirilcolinesterase/metabolismo , Acetilcolinesterase/metabolismo , Triazóis/farmacologia , Triazóis/química , Simulação de Acoplamento Molecular , Relação Estrutura-Atividade , Inibidores da Colinesterase/farmacologia , Inibidores da Colinesterase/química , LigantesRESUMO
Previous biological tests have shown that some resveratrol analogs exhibited significant antioxidative and cholinesterase inhibitory potential, as evidenced by lower IC50 values compared to the established standards, resveratrol and galantamine, respectively. Photochemical transformations were made in parallel on these compounds in the presence of porphyrin photocatalysts in batch and microreactor, showing the significant advantage of flow photochemistry concerning productivity, selectivity, and yields. In this research, the products of photocatalysis and direct irradiation (photolysis) of resveratrol analogs were compared to elucidate how the types and ratios of the products depend on the excitation energy, to reveal the effects of the substituent on the photoinduced reactions and to rationalize experimentally and computationally the nature and ratio of the obtained products. Thus, two main paths were computed in agreement with the experimental results: isomerization with the participation of triplet state intermediates to yield the experimentally detected cis-isomers and subsequent cyclization following a pathway not available for the trans-isomers. The investigation of five model compounds confirmed the advantages of the flow photoreactor in the photochemical reactions of heterocyclic resveratrol analogs.
Assuntos
Antioxidantes , Colinesterases , Resveratrol , Ciclização , GalantaminaRESUMO
A new series of 4H-1,3-benzothiazine dyes were prepared and fully characterized in an aqueous medium. Benzothiazine salts were synthesized either through the classical synthetic pathway using Buchwald-Hartwig amination or through economical and environmentally friendly electrochemical synthesis. The latest synthetic approach employs successful electrochemical intramolecular dehydrogenative cyclization of N-benzylbenzenecarbothioamides to form 4H-1,3-benzothiazines. 4H-1,3-Benzothiazines were evaluated as novel DNA/RNA probes. Through the use of several methods such as UV/vis spectrophotometric titrations, circular dichroism and thermal melting experiments, the binding of four benzothiazine-based molecules to polynucleotides was examined. Compounds 1 and 2 acted as DNA/RNA groove binders, thus suggesting the potential of these compounds as novel DNA/RNA probes. This is a proof-of-concept study and will be expanded to include SAR/QSAR studies.
Assuntos
Corantes , DNA , Sondas RNA , DNA/química , Dicroísmo CircularRESUMO
Naphtho-triazoles and thienobenzo-triazoles have so far proven to be very potent inhibitors of the enzyme butyrylcholinesterase (BChE). Based on these results, in this work, new thienobenzo-thiazoles were designed and synthesized, and their potential inhibitory activity was tested and compared with their analogs, naphtho-oxazoles. The synthesis was carried out by photochemical cyclization of thieno-thiazolostilbenes obtained in the first reaction step. Several thienobenzo-thiazoles and naphtho-oxazoles have shown significant potential as BChE inhibitors, together with the phenolic thiazolostilbene being the most active of all tested compounds. These results are significant as BChE has been attracting growing attention due to its positive role in the treatment of Alzheimer's disease. Computational examination based on the DFT approach enabled the characterization of the geometry and electronic structure of the studied molecules. Furthermore, the molecular docking study, accompanied by additional optimization of complexes ligand-active site, offered insight into the structure and stabilizing interactions in the complexes of studied molecules and BChE.
Assuntos
Butirilcolinesterase , Tiazóis , Butirilcolinesterase/química , Simulação de Acoplamento Molecular , Tiazóis/farmacologia , Tiazóis/química , Oxazóis , Inibidores da Colinesterase/farmacologia , Inibidores da Colinesterase/química , Acetilcolinesterase/metabolismo , Relação Estrutura-AtividadeRESUMO
Photoremovable protecting groups (photocages) 6b-6i based on 1-amino-2-hydroxymethylnaphthalene were developed, and their applicability to release alcohols and carboxylic acids in photohydrolysis was investigated. Compound 6b cannot release alcohol since N-demethylation takes place instead. However, the photorelease of carboxylic acids from 6c-6i was demonstrated on caged substrates, including some nonsteroidal drugs and a neurotransmitter. A simultaneous use of aniline and aminonaphthalene cages allows for the chromatic orthogonality and selective deprotection by UV-B or near-visible and UV-A light, respectively. The photochemical reaction mechanism of decaging was investigated by fluorescence measurements and laser flash photolysis, indicating that the heterolysis and elimination of carboxylic acids take place in the singlet excited state, delivering carbocation as an intermediate. The photoheterolysis in the singlet excited state, which directly releases caged substrates, is highly applicable for the photocages and has advantages compared to hitherto used nitrobenzyl derivatives.
Assuntos
Álcoois , Ácidos Carboxílicos , Álcoois/química , Ácidos Carboxílicos/química , Fluorescência , Luz , FotóliseRESUMO
The acidochromism and acid-base properties of 2,6-distyrylpyridine (2,6-DStP) derivatives bearing on the sides push/pull substituents (namely two dimethylamino, one nitro, and one methoxy and two nitro groups in the case of 2,6-bis[(E)-2-(4-dimetylaminophenyl)ethenyl]pyridine, 2-[(E)-2-(4-nitrophenyl)ethenyl],6-[(E)-2'-(4'-methoxyphenyl)ethenyl]pyridine and 2,6-bis[(E)-2-(4-nitrophenyl)ethenyl]pyridine, respectively) were investigated by stationary and time-resolved spectroscopies. The sensitivity of the absorption and emission spectrum to the medium acidity was found to enhance in the dimethylamino-derivative relative to the unsubstituted 2,6-DStP, also because of the second protonation by the N(CH3)2 group. Spectrophotometric titrations, also processed by a global fitting approach, gave pKa values, for the protonation of the central pyridine, higher in the derivatives with electron-donor unities and lower in compounds bearing electron-acceptor groups. A fluorometric titration was performed in the case of the dimethylamino-derivative thanks to non-negligible emission efficiencies for both neutral and protonated species, unveiling an attractive naked-eye acido(fluoro)chromism from green to yellow upon pyridine protonation, and then to purple with the second protonation involving the lateral N(CH3)2 substituent. Due to the extremely short excited-state lifetimes, as resulted from femtosecond transient absorption experiments, the pKa values for the excited state (pKa*) were estimated through the Förster cycle, revealing that the monoprotonated species of the dimethylamino-derivative would become upon excitation the only stable form in a wide range of pH.
Assuntos
Piridinas , Espectrofotometria , Análise EspectralRESUMO
New 1,2,3-triazolostilbenes were synthesized and photochemically transformed to substituted naphthotriazoles as electrocyclization products in high isolated yields for studying the acetyl- and butyrylcholinesterase inhibitory and anti-inflammatory activity. The best experimental results showed the naphthotriazole photoproducts providing interesting observation on cholinesterase inhibition associated with the inhibition of TNFα cytokine production. The geometries of synthesized triazolostilbenes were computationally examined using Density Functional Theory (DFT), followed by time-dependent DFT calculations to obtain insight into electronic properties observed by UV-Vis spectroscopy. The complexes between selected compounds with the active site of AChE are assessed by docking. A quantum mechanical cluster approach was utilized to optimize their structures, thus providing insight into the stabilizing interactions between the potential inhibitor and the active site.
Assuntos
Acetilcolinesterase , Butirilcolinesterase , Acetilcolinesterase/metabolismo , Butirilcolinesterase/metabolismo , Inibidores da Colinesterase/química , Inibidores da Colinesterase/farmacologia , Simulação de Acoplamento Molecular , Fotoquímica , Relação Estrutura-Atividade , Triazóis/farmacologiaRESUMO
In this research, the synthesis, photochemistry, and computational study of new cis- and trans-isomers of amino-thienostilbenes is performed to test the efficiency of their production and acid resistance, and to investigate their electronic structure, photoreactivity, photophysical characteristics, and potential biological activity. The electronic structure and conformations of synthesized thienostilbene amines and their photocyclization products are examined computationally, along with molecular modeling of amines possessing two thiophene rings that showed inhibitory potential toward cholinesterases. New amino-styryl thiophenes, with favorable photophysical properties and proven acid resistance, represent model compounds for their water-soluble ammonium salts as potential styryl optical dyes. The comparison with organic dyes possessing a trans-aminostilbene subunit as the scaffold shows that the newly synthesized trans-aminostilbenes have very similar absorbance wavelengths. Furthermore, their functionalized cis-isomers and photocyclization products are good candidates for cholinesterase inhibitors because of the structural similarity of the molecular skeleton to some already proven bioactive derivatives.
Assuntos
Benzilaminas , Tiofenos , Fotoquímica , Tiofenos/farmacologia , Modelos Moleculares , CorantesRESUMO
The identification of novel molecular systems with high fluorescence and significant non-linear optical (NLO) properties is a hot topic in the continuous search for new emissive probes. Here, the photobehavior of three two-arm bis[(dimethylamino)styryl]benzene derivatives, where the central benzene was replaced by pyridine, furan, or thiophene, was studied by stationary and time-resolved spectroscopic techniques with ns and fs resolution. The three molecules under investigation all showed positive fluorosolvatochromism, due to intramolecular charge-transfer (ICT) dynamics from the electron-donor dimethylamino groups, and significant fluorescence quantum yields, because of the population of a planar and emissive ICT state stabilized by intramolecular hydrogen-bond-like interactions. The NLO properties (hyperpolarizability coefficient and TPA cross-section) were also measured. The obtained results allowed the role of the central heteroaromatic ring to be disclosed. In particular, the introduction of the thiophene ring guarantees high fluorescent quantum yields irrespective of the polarity of the medium, and the largest hyperpolarizability coefficient because of the increased conjugation. An important and structure-dependent involvement of the triplet state was also highlighted, with the intersystem crossing being competitive with fluorescence, especially in the thiophene derivative, where the triplet was found to significantly sensitize molecular oxygen even in polar environment, leading to possible applications in photodynamic therapy.
Assuntos
Derivados de Benzeno , Oxigênio , Estrutura Molecular , Benzeno , TiofenosRESUMO
New resveratrol-thiophene and resveratrol-maltol hybrids were synthesized as cholinesterase inhibitors and antioxidants. As with photostability experiments, biological tests also found remarkable differences in the properties and behavior of thiophene and maltol hybrids. While resveratrol-thiophene hybrids have excellent inhibitory and antioxidant properties (similar to the activity of reference drug galantamine), maltols have been proven to be weaker inhibitors and antioxidants. The molecular docking of selected active ligands gave insight into the structures of docked enzymes. It enabled the identification of interactions between the ligand and the active site of both cholinesterases. The maltols that proved to be active cholinesterase inhibitors were able to coordinate Fe3+ ion, forming complexes of 1:1 composition. Their formation constants, determined by spectrophotometry, are very similar, lgK = 11.6-12.6, suggesting that Fe3+ binds to the common hydroxy-pyranone moiety and is hardly affected by the other aromatic part of the ligand. Accordingly, the characteristic bands in their individual absorption spectra are uniformly red-shifted relative to those of the free ligands. The crystal structures of two new resveratrol-maltol hybrids were recorded, giving additional information on the molecules' intermolecular hydrogen bonds and packing. In this way, several functionalities of these new resveratrol hybrids were examined as a necessary approach to finding more effective drugs for complicated neurodegenerative diseases.
Assuntos
Doença de Alzheimer , Oligoelementos , Doença de Alzheimer/metabolismo , Antioxidantes/química , Antioxidantes/farmacologia , Quelantes/química , Inibidores da Colinesterase/química , Inibidores da Colinesterase/farmacologia , Colinesterases/metabolismo , Galantamina , Humanos , Ligantes , Simulação de Acoplamento Molecular , Pironas , Resveratrol , Relação Estrutura-Atividade , TiofenosRESUMO
The competition between excited state deactivation processes in mono and double-arm push-pull systems bearing pyridine, furan or thiophene (electron donors) and nitro groups (electron acceptors) was investigated in several solvents through nanosecond and femtosecond transient absorption spectroscopy. Triplet population is the main deactivation pathway for the mono-arm compounds. The large triplet production is mainly ascribed to 3(n,π*) states almost isoenergetic to S1, introduced by nitro groups, as predicted by TD-DFT calculations. The large triplet population may indeed be exploited to produce long-lived excitons for photovoltaic and optoelectronic applications. Two-arm furan and thiophene derivatives instead undergo strong ultrafast intramolecular charge transfer (ICT), which is responsible for their appreciable two-photon absorption cross-sections. In this case, significant fluorescence and singlet oxygen quantum yields are obtained, making these two compounds interesting as potential traceable photosensitizers in photodynamic therapy.
RESUMO
The enzymes acetylcholinesterase (AChE) and butyrylcholinesterase (BChE) are primary targets in attenuating the symptoms of neurodegenerative diseases. Their inhibition results in elevated concentrations of the neurotransmitter acetylcholine which supports communication among nerve cells. It was previously shown for trans-4/5-arylethenyloxazole compounds to have moderate AChE and BChE inhibitory properties. A preliminary docking study showed that elongating oxazole molecules and adding a new NH group could make them more prone to bind to the active site of both enzymes. Therefore, new trans-amino-4-/5-arylethenyl-oxazoles were designed and synthesised by the Buchwald-Hartwig amination of a previously synthesised trans-chloro-arylethenyloxazole derivative. Additionally, naphthoxazole benzylamine photoproducts were obtained by efficient photochemical electrocyclization reaction. Novel compounds were tested as inhibitors of both AChE and BChE. All of the compounds exhibited binding preference for BChE over AChE, especially for trans-amino-4-/5-arylethenyl-oxazole derivatives which inhibited BChE potently (IC50 in µM range) and AChE poorly (IC50â«100 µM). Therefore, due to the selectivity of all of the tested compounds for binding to BChE, these compounds could be applied for further development of cholinesterase selective inhibitors.HIGHLIGHTSSeries of oxazole benzylamines were designed and synthesisedThe tested compounds showed binding selectivity for BChENaphthoxazoles were more potent AChE inhibitors.
Assuntos
Benzilaminas/química , Inibidores da Colinesterase/química , Inibidores da Colinesterase/farmacologia , Desenho de Fármacos , Oxazóis/química , Acetilcolinesterase/efeitos dos fármacos , Butirilcolinesterase/efeitos dos fármacos , Inibidores da Colinesterase/síntese química , Técnicas Eletroquímicas , Concentração Inibidora 50 , Simulação de Acoplamento Molecular , Processos Fotoquímicos , Relação Estrutura-AtividadeRESUMO
A library of amine, oxime, ether, epoxy and acyl derivatives of the benzobicyclo[3.2.1]octene were synthesized and evaluated as inhibitors of both human acetylcholinesterase (AChE) and butyrylcholinesterase (BChE). The majority of the tested compounds exhibited higher selectivity for BChE. Structural adjustment for AChE seems to have been achieved by acylation, and the furan ring opening of furo-benzobicyclo[3.2.1]octadiene results for compound 51 with the highest AChE affinity (IC50 = 8.3 µM). Interestingly, its analogue, an oxime ether with a benzobicyclo[3.2.1]-skeleton, compound 32 was one of the most potent BChE inhibitors in this study (IC50 = 31 µM), but not as potent as endo-43, an ether derivative of the benzobicyclo[3.2.1]octene with an additional phenyl substituent (IC50 = 17 µM). Therefore, we identified several cholinesterase inhibitors with a potential for further development as potential drugs for the treatment of neurodegenerative diseases.
Assuntos
Acetilcolinesterase/metabolismo , Butirilcolinesterase/metabolismo , Inibidores da Colinesterase/síntese química , Ciclo-Octanos/química , Bibliotecas de Moléculas Pequenas/síntese química , Acilação , Benzilaminas/química , Inibidores da Colinesterase/metabolismo , Teoria da Densidade Funcional , Compostos de Epóxi/química , Éter/química , Humanos , Concentração Inibidora 50 , Simulação de Acoplamento Molecular , Oximas/química , Relação Estrutura-AtividadeRESUMO
In order to prepare novel polycyclic derivatives of bicyclo[3.2.1]octadiene systems fused with a thiophene ring, photochemical cyclization and aldol condensation reactions were carried out. The starting substrates were easily obtained by a Vilsmeier-Haack reaction of bicyclo[3.2.1]octadiene thiophene derivatives with dimethylformamide. From the obtained carbaldehydes, novel methyl, methoxy, and cyano-substituted styryl thienobenzobicyclo[3.2.1]octadiene derivatives were synthesized through Wittig reactions and subjected to photochemical cyclization, in terms of obtaining the new annulated structures. As part of this study, the aldol reaction of the starting 2-substituted carbaldehyde with acetone was also performed, which produced the thieno-fused benzobicyclo[3.2.1]octadiene compound with an extended conjugation.