Your browser doesn't support javascript.
loading
Mostrar: 20 | 50 | 100
Resultados 1 - 20 de 29
Filtrar
1.
Proc Natl Acad Sci U S A ; 120(28): e2211251120, 2023 07 11.
Artigo em Inglês | MEDLINE | ID: mdl-37399417

RESUMO

Phylum Tardigrada (water bears), well known for their cryptobiosis, includes small invertebrates with four paired limbs and is divided into two classes: Eutardigrada and Heterotardigrada. The evolutionary origin of Tardigrada is known to lie within the lobopodians, which are extinct soft-bodied worms with lobopodous limbs mostly discovered at sites of exceptionally well-preserved fossils. Contrary to their closest relatives, onychophorans and euarthropods, the origin of morphological characters of tardigrades remains unclear, and detailed comparison with the lobopodians has not been well explored. Here, we present detailed morphological comparison between tardigrades and Cambrian lobopodians, with a phylogenetic analysis encompassing most of the lobopodians and three panarthropod phyla. The results indicate that the ancestral tardigrades likely had a Cambrian lobopodian-like morphology and shared most recent ancestry with the luolishaniids. Internal relationships within Tardigrada indicate that the ancestral tardigrade had a vermiform body shape without segmental plates, but possessed cuticular structures surrounding the mouth opening, and lobopodous legs terminating with claws, but without digits. This finding is in contrast to the long-standing stygarctid-like ancestor hypothesis. The highly compact and miniaturized body plan of tardigrades evolved after the tardigrade lineage diverged from an ancient shared ancestor with the luolishaniids.


Assuntos
Artrópodes , Tardígrados , Animais , Tardígrados/genética , Filogenia , Evolução Biológica , Invertebrados , Fósseis
2.
Dev Biol ; 497: 42-58, 2023 05.
Artigo em Inglês | MEDLINE | ID: mdl-36893882

RESUMO

Primordial germ cells (PGCs) give rise to gametes - cells necessary for the propagation and fertility of diverse organisms. Current understanding of PGC development is limited to the small number of organisms whose PGCs have been identified and studied. Expanding the field to include little-studied taxa and emerging model organisms is important to understand the full breadth of the evolution of PGC development. In the phylum Tardigrada, no early cell lineages have been identified to date using molecular markers. This includes the PGC lineage. Here, we describe PGC development in the model tardigrade Hypsibius exemplaris. The four earliest-internalizing cells (EICs) exhibit PGC-like behavior and nuclear morphology. The location of the EICs is enriched for mRNAs of conserved PGC markers wiwi1 (water bear piwi 1) and vasa. At early stages, both wiwi1 and vasa mRNAs are detectable uniformly in embryos, which suggests that these mRNAs do not serve as localized determinants for PGC specification. Only later are wiwi1 and vasa enriched in the EICs. Finally, we traced the cells that give rise to the four PGCs. Our results reveal the embryonic origin of the PGCs of H. exemplaris and provide the first molecular characterization of an early cell lineage in the tardigrade phylum. We anticipate that these observations will serve as a basis for characterizing the mechanisms of PGC development in this animal.


Assuntos
Tardígrados , Animais , Células Germinativas , RNA Mensageiro/genética
3.
Evol Dev ; : e12457, 2023 Sep 18.
Artigo em Inglês | MEDLINE | ID: mdl-37721221

RESUMO

Tardigrada is an ancient lineage of miniaturized animals. As an outgroup of the well-studied Arthropoda and Onychophora, studies of tardigrades hold the potential to reveal important insights into body plan evolution in Panarthropoda. Previous studies have revealed interesting facets of tardigrade development and genomics that suggest that a highly compact body plan is a derived condition of this lineage, rather than it representing an ancestral state of Panarthropoda. This conclusion was based on studies of several species from Eutardigrada. We review these studies and expand on them by analyzing the publicly available genome and transcriptome assemblies of Echiniscus testudo, a representative of Heterotardigrada. These new analyses allow us to phylogenetically reconstruct important features of genome evolution in Tardigrada. We use available data from tardigrades to interrogate several recent models of body plan evolution in Panarthropoda. Although anterior segments of panarthropods are highly diverse in terms of anatomy and development, both within individuals and between species, we conclude that a simple one-to-one alignment of anterior segments across Panarthropoda is the best available model of segmental homology. In addition to providing important insight into body plan diversification within Panarthropoda, we speculate that studies of tardigrades may reveal generalizable pathways to miniaturization.

4.
Proc Biol Sci ; 287(1931): 20201135, 2020 07 29.
Artigo em Inglês | MEDLINE | ID: mdl-33043863

RESUMO

Tardigrades have a miniaturized body plan. Miniaturization in tardigrades is associated with the loss of several organ systems and an intermediate region of their anteroposterior (AP) axis. However, how miniaturization has affected tardigrade legs is unclear. In arthropods and in onychophorans, the leg gap genes are expressed in regionalized proximodistal (PD) patterns in the legs. Functional studies indicate that these genes regulate growth in their respective expression domains and establish PD identities, partly through mutually antagonistic regulatory interactions. Here, we investigated the expression patterns of tardigrade orthologs of the leg gap genes. Rather than being restricted to a proximal leg region, as in arthropods and onychophorans, we detected coexpression of orthologues of homothorax and extradenticle broadly across the legs of the first three trunk segments in the tardigrade Hypsibius exemplaris. We could not identify a dachshund orthologue in tardigrade genomes, a gene that is expressed in an intermediate region of developing legs in arthropods and onychophorans, suggesting that this gene was lost in the tardigrade lineage. We detected Distal-less expression broadly across all developing leg buds in H. exemplaris embryos, unlike in arthropods and onychophorans, in which it exhibits a distally restricted expression domain. The broad expression patterns of the remaining leg gap genes in H. exemplaris legs may reflect the loss of dachshund and the accompanying loss of an intermediate region of the legs in the tardigrade lineage. We propose that the loss of intermediate regions of both the AP and PD body axes contributed to miniaturization of Tardigrada.


Assuntos
Tamanho Corporal , Tardígrados/fisiologia , Animais , Evolução Biológica , Miniaturização , Fenótipo
5.
Proc Natl Acad Sci U S A ; 112(52): 15976-81, 2015 Dec 29.
Artigo em Inglês | MEDLINE | ID: mdl-26598659

RESUMO

Horizontal gene transfer (HGT), or the transfer of genes between species, has been recognized recently as more pervasive than previously suspected. Here, we report evidence for an unprecedented degree of HGT into an animal genome, based on a draft genome of a tardigrade, Hypsibius dujardini. Tardigrades are microscopic eight-legged animals that are famous for their ability to survive extreme conditions. Genome sequencing, direct confirmation of physical linkage, and phylogenetic analysis revealed that a large fraction of the H. dujardini genome is derived from diverse bacteria as well as plants, fungi, and Archaea. We estimate that approximately one-sixth of tardigrade genes entered by HGT, nearly double the fraction found in the most extreme cases of HGT into animals known to date. Foreign genes have supplemented, expanded, and even replaced some metazoan gene families within the tardigrade genome. Our results demonstrate that an unexpectedly large fraction of an animal genome can be derived from foreign sources. We speculate that animals that can survive extremes may be particularly prone to acquiring foreign genes.


Assuntos
Transferência Genética Horizontal , Genoma/genética , Biblioteca Genômica , Análise de Sequência de DNA/métodos , Tardígrados/genética , Animais , DNA Arqueal/química , DNA Arqueal/genética , DNA Bacteriano/química , DNA Bacteriano/genética , DNA Fúngico/química , DNA Fúngico/genética , DNA de Plantas/química , DNA de Plantas/genética , DNA Viral/química , DNA Viral/genética , Filogenia , Tardígrados/classificação
6.
Dev Biol ; 395(1): 182-97, 2014 Nov 01.
Artigo em Inglês | MEDLINE | ID: mdl-25195194

RESUMO

The establishment of segment identity is a key developmental process that allows for divergence along the anteroposterior body axis in arthropods. In Drosophila, the identity of a segment is determined by the complement of Hox genes it expresses. In many contexts, Hox transcription factors require the protein products of extradenticle (exd) and homothorax (hth) as cofactors to perform their identity specification functions. In holometabolous insects, segment identity may be specified twice, during embryogenesis and metamorphosis. To glean insight into the relationship between embryonic and metamorphic segmental identity specification, we have compared these processes in the flour beetle Tribolium castaneum, which develops ventral appendages during embryogenesis that later metamorphose into adult appendages with distinct morphologies. At metamorphosis, comparisons of RNAi phenotypes indicate that Hox genes function jointly with Tc-hth and Tc-exd to specify several region-specific aspects of the adult body wall. On the other hand, Hox genes specify appendage identities along the anteroposterior axis independently of Tc-hth/Tc-exd and Tc-hth/Tc-exd specify proximal vs. distal identity within appendages independently of Hox genes during this stage. During embryogenesis, Tc-hth and Tc-exd play a broad role in the segmentation process and are required for specification of body wall identities in the thorax; however, contrasting with results from other species, we did not obtain homeotic transformations of embryonic appendages in response to Tc-hth or Tc-exd RNAi. In general, the homeotic effects of interference with the function of Hox genes and Tc-hth/Tc-exd during metamorphosis did not match predictions based on embryonic roles of these genes. Comparing metamorphic patterning in T. castaneum to embryonic and post-embryonic development in hemimetabolous insects suggests that holometabolous metamorphosis combines patterning processes of both late embryogenesis and metamorphosis of the hemimetabolous life cycle.


Assuntos
Padronização Corporal/genética , Proteínas de Homeodomínio/genética , Proteínas de Insetos/genética , Metamorfose Biológica/genética , Tribolium/genética , Estruturas Animais/crescimento & desenvolvimento , Estruturas Animais/metabolismo , Animais , Regulação da Expressão Gênica no Desenvolvimento , Genes Homeobox/genética , Larva/genética , Larva/crescimento & desenvolvimento , Larva/ultraestrutura , Microscopia Eletrônica de Varredura , Fenótipo , Interferência de RNA , Reação em Cadeia da Polimerase Via Transcriptase Reversa , Especificidade da Espécie , Tribolium/embriologia , Tribolium/crescimento & desenvolvimento
7.
Evol Dev ; 16(2): 78-91, 2014 Mar.
Artigo em Inglês | MEDLINE | ID: mdl-24617987

RESUMO

The arthropod labrum is an anterior appendage-like structure that forms the dorsal side of the preoral cavity. Conflicting interpretations of fossil, nervous system, and developmental data have led to a proliferation of scenarios for labral evolution. The best supported hypothesis is that the labrum is a novel structure that shares development with appendages as a result of co-option. Here, we use RNA interference in the red flour beetle Tribolium castaneum to compare metamorphic patterning of the labrum to previously published data on ventral appendage patterning. As expected under the co-option hypothesis, depletion of several genes resulted in similar defects in the labrum and ventral appendages. These include proximal deletions and proximal-to-distal transformations resulting from depletion of the leg gap genes homothorax and extradenticle, large-scale deletions resulting from depletion of the leg gap gene Distal-less, and smaller distal deletions resulting from knockdown of the EGF ligand Keren. However, depletion of dachshund and many of the genes that function downstream of the leg gap genes in the ventral appendages had either subtle or no effects on labral axis patterning. This pattern of partial similarity suggests that upstream genes act through different downstream targets in the labrum. We also discovered that many appendage axis patterning genes have roles in patterning the epipharyngeal sensillum array, suggesting that they have become integrated into a novel regulatory network. These genes include Notch, Delta, and decapentaplegic, and the transcription factors abrupt, bric à brac, homothorax, extradenticle and the paralogs apterous a and apterous b.


Assuntos
Proteínas de Insetos/genética , Tribolium/crescimento & desenvolvimento , Tribolium/genética , Animais , Padronização Corporal , Regulação da Expressão Gênica no Desenvolvimento , Proteínas de Insetos/metabolismo , Interferência de RNA , Receptores Notch/metabolismo , Transdução de Sinais , Fatores de Transcrição/metabolismo , Tribolium/citologia
8.
bioRxiv ; 2023 Feb 15.
Artigo em Inglês | MEDLINE | ID: mdl-36824831

RESUMO

Primordial germ cells (PGCs) give rise to gametes â€" cells necessary for the propagation and fertility of diverse organisms. Current understanding of PGC development is limited to the small number of organisms whose PGCs have been identified and studied. Expanding the field to include little-studied taxa and emerging model organisms is important to understand the full breadth of the evolution of PGC development. In the phylum Tardigrada, no early cell lineages have been identified to date using molecular markers. This includes the PGC lineage. Here, we describe PGC development in the model tardigrade Hypsibius exemplaris . The four earliest-internalizing cells (EICs) exhibit PGC-like behavior and nuclear morphology. The location of the EICs is enriched for mRNAs of conserved PGC markers wiwi1 (water bear piwi 1) and vasa . At early stages, both wiwi1 and vasa mRNAs are detectable uniformly in embryos, which suggests that these mRNAs do not serve as localized determinants for PGC specification. Only later are wiwi1 and vasa enriched in the EICs. Finally, we traced the cells that give rise to the four PGCs. Our results reveal the embryonic origin of the PGCs of H. exemplaris and provide the first molecular characterization of an early cell lineage in the tardigrade phylum. We anticipate that these observations will serve as a basis for characterizing the mechanisms of PGC development in this animal.

9.
Dev Biol ; 360(2): 369-80, 2011 Dec 15.
Artigo em Inglês | MEDLINE | ID: mdl-21996282

RESUMO

Genitalia play an important role in the life histories of insects, as in other animals. These sexually dimorphic structures evolve rapidly and derive from multiple body segments. Despite the importance of insect genitalia, descriptions of their genetic patterning have been limited to fruit flies. In this study, we report the functions, interactions and regulation of appendage patterning genes (e.g. homothorax, dachshund, and Distal-less) in two insects: the milkweed bug Oncopeltus fasciatus, and the red flour beetle Tribolium castaneum. These species differ in the anatomical complexity of their genitalia. Females of T. castaneum have a terminal ovipositor ending in short styli, while O. fasciatus have a multi-jointed subterminal ovipositor. Male O. fasciatus have a genital capsule consisting of large gonocoxopodites and claspers; T. castaneum males have relatively simple genitalia. The requirement of appendage-patterning genes in males differed between the two species: No defects were observed in T. castaneum male genitalia, and while the male claspers of O. fasciatus were affected by depletion of appendage-patterning genes, the proximal gonocoxopodite was not, suggesting a non-appendicular origin for this structure. Only the styli of the T. castaneum ovipositor were affected by RNAi depletion of appendage-patterning genes (14 genes in all). The posterior Hox genes (abdominal-A and Abdominal-B) were required for proper genital development in O. fasciatus and regulated Distal-less and homothorax similarly in both sexes. Distal-less and dachshund were regulated differently in male and female O. fasciatus. Knockdown of the sex determination gene intersex produced a partial female-to-male transformation of abdominal and genital anatomy and also resulted in abrogation of female-specific regulation of these genes. These results provide developmental genetic support for specific anatomical hypotheses of serial homology. Importantly, these gene functions and interactions describe the developmental patterning of sexually dimorphic structures that have been critical to the diversification of these species-rich insect groups.


Assuntos
Padronização Corporal/genética , Regulação da Expressão Gênica no Desenvolvimento , Genes de Insetos , Heterópteros/genética , Proteínas de Homeodomínio/genética , Proteínas de Insetos/genética , Tribolium/genética , Animais , Extremidades/crescimento & desenvolvimento , Feminino , Genitália/crescimento & desenvolvimento , Heterópteros/crescimento & desenvolvimento , Proteínas de Homeodomínio/metabolismo , Proteínas de Insetos/metabolismo , Masculino , Dados de Sequência Molecular , Tribolium/crescimento & desenvolvimento
10.
BMC Ecol Evol ; 21(1): 223, 2021 12 27.
Artigo em Inglês | MEDLINE | ID: mdl-34961481

RESUMO

BACKGROUND: Wnt genes code for ligands that activate signaling pathways during development in Metazoa. Through the canonical Wnt (cWnt) signaling pathway, these genes regulate important processes in bilaterian development, such as establishing the anteroposterior axis and posterior growth. In Arthropoda, Wnt ligands also regulate segment polarity, and outgrowth and patterning of developing appendages. Arthropods are part of a lineage called Panarthropoda that includes Onychophora and Tardigrada. Previous studies revealed potential roles of Wnt genes in regulating posterior growth, segment polarity, and growth and patterning of legs in Onychophora. Unlike most other panarthropods, tardigrades lack posterior growth, but retain segmentation and appendages. Here, we investigated Wnt genes in tardigrades to gain insight into potential roles that these genes play during development of the highly compact and miniaturized tardigrade body plan. RESULTS: We analyzed published genomes for two representatives of Tardigrada, Hypsibius exemplaris and Ramazzottius varieornatus. We identified single orthologs of Wnt4, Wnt5, Wnt9, Wnt11, and WntA, as well as two Wnt16 paralogs in both tardigrade genomes. We only found a Wnt2 ortholog in H. exemplaris. We could not identify orthologs of Wnt1, Wnt6, Wnt7, Wnt8, or Wnt10. We identified most other components of cWnt signaling in both tardigrade genomes. However, we were unable to identify an ortholog of arrow/Lrp5/6, a gene that codes for a Frizzled co-receptor of Wnt ligands. Additionally, we found that some other animals that have lost several Wnt genes and are secondarily miniaturized, like tardigrades, are also missing an ortholog of arrow/Lrp5/6. We analyzed the embryonic expression patterns of Wnt genes in H. exemplaris during developmental stages that span the establishment of the AP axis through segmentation and leg development. We detected expression of all Wnt genes in H. exemplaris besides one of the Wnt16 paralogs. During embryo elongation, expression of several Wnt genes was restricted to the posterior pole or a region between the anterior and posterior poles. Wnt genes were expressed in distinct patterns during segmentation and development of legs in H. exemplaris, rather than in broadly overlapping patterns. CONCLUSIONS: Our results indicate that Wnt signaling has been highly modified in Tardigrada. While most components of cWnt signaling are conserved in tardigrades, we conclude that tardigrades have lost Wnt1, Wnt6, Wnt7, Wnt8, and Wnt10, along with arrow/Lrp5/6. Our expression data may indicate a conserved role of Wnt genes in specifying posterior identities during establishment of the AP axis. However, the loss of several Wnt genes and the distinct expression patterns of Wnt genes during segmentation and leg development may indicate that combinatorial interactions among Wnt genes are less important during tardigrade development compared to many other animals. Based on our results, and comparisons to previous studies, we speculate that the loss of several Wnt genes in Tardigrada may be related to a reduced number of cells and simplified development that accompanied miniaturization and anatomical simplification in this lineage.


Assuntos
Artrópodes , Tardígrados , Animais , Artrópodes/genética , Receptores Frizzled/genética , Genoma , Ligantes , Tardígrados/genética
11.
Ann Bot ; 101(7): 971-82, 2008 May.
Artigo em Inglês | MEDLINE | ID: mdl-18292604

RESUMO

BACKGROUND AND AIMS: Plant growth responses to the rare earth elements lanthanum (La) and cerium (Ce) have been reported, but little is known about the effects of these two elements on plant mineral nutrition. METHODS: Corn (Zea mays 'Hycorn 82') and mungbean (Vigna radiata 'Berken') were grown in continuous flowing nutrient solutions containing 0, 0.2, 1.0 and 5.0 microm La or Ce. At harvest plants were divided into roots and shoots, dried, weighed and analysed for macro- and micronutrients, as well as for La and Ce. KEY RESULTS: La and Ce did not increase the growth of corn or mungbean. The dry weight of corn shoots was decreased by 32 % in the presence of 5.0 microm Ce; the other La and Ce concentrations had no effect. La and Ce concentrations of 0.9 and 5.0 microm decreased the shoot dry weight of mungbean by 75 or 95 %, the two elements having closely similar effects. Decreases in the uptake of Ca, Na, Zn and Mn by corn were observed with increases in solution La and Ce. For mungbean, the uptake rates of all measured elements decreased with increases in solution La and Ce. The concentrations of La and Ce in the roots of both species were higher than in the shoots and increased strongly with increasing concentrations of La or Ce in solution. The La and Ce concentrations in mungbean shoots were always higher than in corn shoots. CONCLUSIONS: La and Ce did not enhance the growth of corn or mungbean, but decreased the growth, root function and consequently the nutritional status of mungbean at concentrations >0.2 microm in solution. It is concluded that if La or Ce have positive effects on corn and mungbean growth, they can only occur at solution concentrations below 0.2 microm.


Assuntos
Cério/farmacologia , Fabaceae/efeitos dos fármacos , Lantânio/farmacologia , Zea mays/efeitos dos fármacos , Cério/metabolismo , Fabaceae/crescimento & desenvolvimento , Fabaceae/metabolismo , Lantânio/metabolismo , Raízes de Plantas/efeitos dos fármacos , Raízes de Plantas/crescimento & desenvolvimento , Raízes de Plantas/metabolismo , Brotos de Planta/efeitos dos fármacos , Brotos de Planta/crescimento & desenvolvimento , Brotos de Planta/metabolismo , Zea mays/crescimento & desenvolvimento , Zea mays/metabolismo
12.
Cold Spring Harb Protoc ; 2018(11)2018 11 01.
Artigo em Inglês | MEDLINE | ID: mdl-30385673

RESUMO

In situ hybridization is a method for visualizing embryonic gene expression that is amenable to nonmodel systems. Here, an in situ hybridization protocol is presented for the tardigrade Hypsibius exemplaris This method allows gene expression to be visualized directly and with fluorescence microscopy.


Assuntos
Embrião não Mamífero/metabolismo , Regulação da Expressão Gênica no Desenvolvimento , Hibridização In Situ/métodos , Tardígrados/genética , Animais , Embrião não Mamífero/embriologia , Imuno-Histoquímica , Microscopia Confocal , Tardígrados/embriologia
13.
Cold Spring Harb Protoc ; 2018(11)2018 11 01.
Artigo em Inglês | MEDLINE | ID: mdl-30385672

RESUMO

Immunostaining is a method used to visualize the localization of proteins in fixed tissue. Many antibodies are available that recognize specific proteins in a wide diversity of organisms, which makes this method ideal for investigating gene expression patterns in nonmodel animal systems. This protocol describes immunostaining for studies of embryogenesis in the tardigrade Hypsibius exemplaris.


Assuntos
Embrião não Mamífero/metabolismo , Desenvolvimento Embrionário , Imuno-Histoquímica/métodos , Tardígrados/embriologia , Animais , Embrião não Mamífero/diagnóstico por imagem , Embrião não Mamífero/embriologia , Microscopia de Fluorescência/métodos
14.
Evodevo ; 9: 19, 2018.
Artigo em Inglês | MEDLINE | ID: mdl-30069303

RESUMO

BACKGROUND: Both euarthropods and vertebrates have tripartite brains. Several orthologous genes are expressed in similar regionalized patterns during brain development in both vertebrates and euarthropods. These similarities have been used to support direct homology of the tripartite brains of vertebrates and euarthropods. If the tripartite brains of vertebrates and euarthropods are homologous, then one would expect other taxa to share this structure. More generally, examination of other taxa can help in tracing the evolutionary history of brain structures. Tardigrades are an interesting lineage on which to test this hypothesis because they are closely related to euarthropods, and whether they have a tripartite brain or unipartite brain has recently been a focus of debate. RESULTS: We tested this hypothesis by analyzing the expression patterns of six3, orthodenticle, pax6, unplugged, and pax2/5/8 during brain development in the tardigrade Hypsibius exemplaris-formerly misidentified as Hypsibius dujardini. These genes were expressed in a staggered anteroposterior order in H. exemplaris, similar to what has been reported for mice and flies. However, only six3, orthodenticle, and pax6 were expressed in the developing brain. Unplugged was expressed broadly throughout the trunk and posterior head, before the appearance of the nervous system. Pax2/5/8 was expressed in the developing central and peripheral nervous system in the trunk. CONCLUSION: Our results buttress the conclusion of our previous study of Hox genes-that the brain of tardigrades is only homologous to the protocerebrum of euarthropods. They support a model based on fossil evidence that the last common ancestor of tardigrades and euarthropods possessed a unipartite brain. Our results are inconsistent with the hypothesis that the tripartite brain of euarthropods is directly homologous to the tripartite brain of vertebrates.

15.
Arthropod Struct Dev ; 46(3): 328-340, 2017 May.
Artigo em Inglês | MEDLINE | ID: mdl-27725256

RESUMO

The origin and diversification of segmented metazoan body plans has fascinated biologists for over a century. The superphylum Panarthropoda includes three phyla of segmented animals-Euarthropoda, Onychophora, and Tardigrada. This superphylum includes representatives with relatively simple and representatives with relatively complex segmented body plans. At one extreme of this continuum, euarthropods exhibit an incredible diversity of serially homologous segments. Furthermore, distinct tagmosis patterns are exhibited by different classes of euarthropods. At the other extreme, all tardigrades share a simple segmented body plan that consists of a head and four leg-bearing segments. The modular body plans of panarthropods make them a tractable model for understanding diversification of animal body plans more generally. Here we review results of recent morphological and developmental studies of tardigrade segmentation. These results complement investigations of segmentation processes in other panarthropods and paleontological studies to illuminate the earliest steps in the evolution of panarthropod body plans.


Assuntos
Artrópodes/anatomia & histologia , Padronização Corporal , Tardígrados/anatomia & histologia , Animais , Biodiversidade , Evolução Biológica , Padronização Corporal/fisiologia
16.
Integr Comp Biol ; 57(3): 546-559, 2017 09 01.
Artigo em Inglês | MEDLINE | ID: mdl-28957526

RESUMO

Incredibly disparate brain types are found in Metazoa, which raises the question of how this disparity evolved. Ecdysozoa includes representatives that exhibit ring-like brains-the Cycloneuralia-and representatives that exhibit ganglionic brains-the Panarthropoda (Euarthropoda, Onychophora, and Tardigrada). The evolutionary steps leading to these distinct brain types are unclear. Phylogenomic analyses suggest that the enigmatic Tardigrada is a closely related outgroup of a Euarthropoda + Onychophora clade; as such, the brains of tardigrades may provide insight into the evolution of ecdysozoan brains. Recently, evolutionarily salient questions have arisen regarding the composition of the tardigrade brain. To address these questions, we investigated brain anatomy in four tardigrade species-Hypsibius dujardini, Milnesium n. sp., Echiniscus n. sp., and Batillipes n. sp.-that together span Tardigrada. Our results suggest that general brain morphology is conserved across Tardigrada. Based on our results we present a hypothesis that proposes direct parallels between the tardigrade brain and the segmental trunk ganglia of the tardigrade ventral nervous system. In this hypothesis, brain neuropil nearly circumscribes the tardigrade foregut. We suggest that the tardigrade brain retains aspects of an ancestral cycloneuralian brain, while exhibiting ganglionic structure characteristic of euarthropods and onychophorans.


Assuntos
Evolução Biológica , Tardígrados/anatomia & histologia , Tardígrados/classificação , Animais , Encéfalo/anatomia & histologia , Filogenia , Tardígrados/embriologia
17.
Curr Biol ; 26(2): 224-229, 2016 Jan 25.
Artigo em Inglês | MEDLINE | ID: mdl-26776737

RESUMO

The superphylum Panarthropoda (Arthropoda, Onychophora, and Tardigrada) exhibits a remarkable diversity of segment morphologies, enabling these animals to occupy diverse ecological niches. The molecular identities of these segments are specified by Hox genes and other axis patterning genes during development [1, 2]. Comparisons of molecular segment identities between arthropod and onychophoran species have yielded important insights into the origins and diversification of their body plans [3-9]. However, the relationship of the segments of tardigrades to those of arthropods and onychophorans has remained enigmatic [10, 11], limiting our understanding of early panarthropod body plan diversification. Here, we reveal molecular identities for all of the segments of a tardigrade. Based on our analysis, we conclude that tardigrades have lost a large intermediate region of the body axis-a region corresponding to the entire thorax and most of the abdomen of insects-and that they have lost the Hox genes that originally specified this region. Our data suggest that nearly the entire tardigrade body axis is homologous to just the head region of arthropods. Based on our results, we reconstruct a last common ancestor of Panarthropoda that had a relatively elongate body plan like most arthropods and onychophorans, rather than a compact, tardigrade-like body plan. These results demonstrate that the body plan of an animal phylum can originate by the loss of a large part of the body.


Assuntos
Genes Homeobox/genética , Cabeça/crescimento & desenvolvimento , Filogenia , Tardígrados/metabolismo , Animais , Tardígrados/genética
18.
Nat Ecol Evol ; 4(12): 1580-1581, 2020 12.
Artigo em Inglês | MEDLINE | ID: mdl-33262516
19.
Mech Dev ; 132: 13-27, 2014 May.
Artigo em Inglês | MEDLINE | ID: mdl-24534744

RESUMO

The antenna was the first arthropod ventral appendage to evolve non-leg identity. Models of antennal evolution have been based on comparisons of antennal and leg identity specification mechanisms in Drosophila melanogaster, a species in which appendages develop from highly derived imaginal discs during the larval period. We test for conservation of the Drosophila antennal identity specification mechanism at metamorphosis in Tribolium castaneum and three other flour beetle species (Tribolium confusum, Tribolium brevicornis and Latheticus oryzae) in the family Tenebrionidae. In Drosophila, loss of function of four transcription factors-homothorax, extradenticle, Distal-less, and spineless-causes large-scale transformations of the antenna to leg identity. Distal-less and spineless function similarly during metamorphosis in T. castaneum. RNA interference (RNAi) targeting homothorax (hth) or extradenticle (exd) caused transformation of the proximal antenna to distal leg identity in flour beetles, but did not affect the identity of the distal antenna. This differs from the functional domain of these genes in early instar Drosophila, where they are required for identity specification throughout the antenna, but matches their functional domain in late instar Drosophila. The similarities between antennal identity specification at metamorphosis in flour beetles and in late larval Drosophila likely reflect the conservation of an ancestral metamorphic developmental mechanism. There were two notable differences in hth/exd loss of function phenotypes between flies and beetles. Flour beetles retained all of their primary segments in both the antenna and legs, whereas flies undergo reduction and fusion of primary segments. This difference in ground state appendage morphology casts doubt on interpretations of developmental ground states as evolutionary atavisms. Additionally, adult Tribolium eyes were transformed to elytron-like structures; we provide a developmental hypothesis for this evolutionarily surprising transformation.


Assuntos
Besouros/genética , Extremidades/crescimento & desenvolvimento , Metamorfose Biológica/genética , Animais , Evolução Biológica , Padronização Corporal/genética , Drosophila/genética , Proteínas de Drosophila/genética , Regulação da Expressão Gênica no Desenvolvimento/genética , Proteínas de Insetos/genética , Fatores de Transcrição/genética , Tribolium/genética
20.
G3 (Bethesda) ; 2(2): 235-48, 2012 Feb.
Artigo em Inglês | MEDLINE | ID: mdl-22384402

RESUMO

Serial homologs are similar structures that develop at different positions within a body plan. These structures share some, but not all, aspects of developmental patterning, and their evolution is thought to be constrained by shared, pleiotropic gene functions. Here we describe the functions of 17 developmental genes during metamorphic development of the legs in the red flour beetle, Tribolium castaneum. This study provides informative comparisons between appendage development in Drosophila melanogaster and T. castaneum, between embryonic and adult development in T. castaneum, and between the development of serially homologous appendages. The leg gap genes Distal-less and dachshund are conserved in function. Notch signaling, the zinc-finger transcription factors related to odd-skipped, and bric-à-brac have conserved functions in promoting joint development. homothorax knockdown alters the identity of proximal leg segments but does not reduce growth. Lim1 is required for intermediate leg development but not distal tarsus and pretarsus development as in D. melanogaster. Development of the tarsus requires decapentaplegic, rotund, spineless, abrupt, and bric-à-brac and the EGF ligand encoded by Keren. Metathoracic legs of T. castaneum have four tarsomeres, whereas other legs have five. Patterns of gene activity in the tarsus suggest that patterning in the middle of the tarsal region, not the proximal- or distal-most areas, is responsible for this difference in segment number. Through comparisons with other recent studies of T. castaneum appendage development, we test hypotheses for the modularity or interdependence of development during evolution of serial homologs.

SELEÇÃO DE REFERÊNCIAS
DETALHE DA PESQUISA