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1.
Mem Inst Oswaldo Cruz ; 108(7): 921-3, 2013 Nov.
Artigo em Inglês | MEDLINE | ID: mdl-24037110

RESUMO

Since the emergence of West Nile virus (WNV) in North America in 1999, there have been several reports of WNV activity in Central and South American countries. To detect WNV in Brazil, we performed a serological survey of horses from different regions of Brazil using recombinant peptides from domain III of WNV. Positive samples were validated with the neutralisation test. Our results showed that of 79 ELISA-positive horses, nine expressed WNV-specific neutralising antibodies. Eight of the infected horses were from the state of Mato Grosso do Sul and one was from the state of Paraíba. Our results provide additional evidence for the emergence of WNV in Brazil and for its circulation in multiple regions of the country.


Assuntos
Anticorpos Antivirais/sangue , Doenças dos Cavalos/epidemiologia , Febre do Nilo Ocidental/veterinária , Vírus do Nilo Ocidental/imunologia , Animais , Brasil/epidemiologia , Ensaio de Imunoadsorção Enzimática/veterinária , Doenças dos Cavalos/diagnóstico , Doenças dos Cavalos/virologia , Cavalos , Testes de Neutralização , Estudos Soroepidemiológicos , Febre do Nilo Ocidental/diagnóstico , Febre do Nilo Ocidental/epidemiologia , Vírus do Nilo Ocidental/isolamento & purificação
2.
Res Vet Sci ; 143: 20-27, 2022 Mar.
Artigo em Inglês | MEDLINE | ID: mdl-34954568

RESUMO

Cost of transport (COT) and monocarboxylate transporters (MCTs) could affect the ability to perform fast actions during a jumping discipline. This study aimed to compare the COT and evaluate the MCT1, MCT4, and their auxiliary protein CD147 content in the gluteus medius and RBCs of Brazilian sport horses (BH), a breed developed for jumping competitions, with low-level (LL) or intermediate-level (IL) jumping capacities. The physiological difference between the horses was assessed by an incremental jump test (IJT), in which the cost of lactate (COTLAC) and heart rate (COTHR) of running were determined for each animal by the ratio between each variable and the running speed. Western blotting was performed on muscle and RBC membranes to quantify MCT1, MCT4, and CD147. IL showed lower COTLAC and COTHR than LL at all jumping heights. The amount of MCT1, MCT4, and CD147 found in muscle and RBCs were not dependent on performance level. Muscle MCT4 and MCT1 were correlated positively with CD147. We conclude that the relatively small differences between performances did not relevantly influence MCT expression in BH. While MCT analyses are inaccessible for most trainers and veterinarians, the cost of transport measurements is a feasible and sensitive tool to distinguish intermediate and low-level jumping horses.


Assuntos
Eritrócitos , Transportadores de Ácidos Monocarboxílicos , Animais , Diferenciação Celular , Contagem de Eritrócitos/veterinária , Eritrócitos/metabolismo , Cavalos , Transportadores de Ácidos Monocarboxílicos/metabolismo , Músculo Esquelético/metabolismo
3.
Rev Soc Bras Med Trop ; 47(4): 414-7, 2014 Jul.
Artigo em Inglês | MEDLINE | ID: mdl-25229279

RESUMO

INTRODUCTION: Arboviruses are an important public health problem in Brazil, in especially flaviviruses, including the Saint Louis encephalitis virus (SLEV) and the Rocio virus (ROCV), are especially problematic. These viruses are transmitted to humans or other vertebrates through arthropod bites and may cause diseases with clinical manifestations that range from asymptomatic infection, viral hemorrhagic fever to encephalitis. METHODS: A serological survey of horses from various regions of Brazil using an enzyme-linked immunosorbent assay (ELISA) with recombinant SLEV domain III peptides and ROCV E protein as antigens. RESULTS: Overall, 415 (55.1%) of the 753 horses that were screened were seropositive for flavivirus and, among them, monotypic reactions were observed to SLEV in 93 (12.3%) and to ROCV in 46 (6.1%). These results suggested that these viruses, or other closely related viruses, are infecting horses in Brazil. However, none of the studied horses presented central nervous system infection symptoms. CONCLUSIONS: Our results suggest that SLEV and ROCV previously circulated among horses in northeast, west-central and southeast Brazil.


Assuntos
Anticorpos Antivirais/sangue , Vírus da Encefalite de St. Louis/imunologia , Encefalite de St. Louis/veterinária , Infecções por Flavivirus/veterinária , Doenças dos Cavalos/epidemiologia , Animais , Brasil/epidemiologia , Encefalite de St. Louis/diagnóstico , Encefalite de St. Louis/epidemiologia , Ensaio de Imunoadsorção Enzimática , Infecções por Flavivirus/diagnóstico , Infecções por Flavivirus/epidemiologia , Doenças dos Cavalos/diagnóstico , Cavalos , Estudos Soroepidemiológicos
4.
Rev. Soc. Bras. Med. Trop ; Rev. Soc. Bras. Med. Trop;47(4): 414-417, Jul-Aug/2014. tab
Artigo em Inglês | LILACS | ID: lil-722307

RESUMO

Introduction Arboviruses are an important public health problem in Brazil, in especially flaviviruses, including the Saint Louis encephalitis virus (SLEV) and the Rocio virus (ROCV), are especially problematic. These viruses are transmitted to humans or other vertebrates through arthropod bites and may cause diseases with clinical manifestations that range from asymptomatic infection, viral hemorrhagic fever to encephalitis. Methods A serological survey of horses from various regions of Brazil using an enzyme-linked immunosorbent assay (ELISA) with recombinant SLEV domain III peptides and ROCV E protein as antigens. Results Overall, 415 (55.1%) of the 753 horses that were screened were seropositive for flavivirus and, among them, monotypic reactions were observed to SLEV in 93 (12.3%) and to ROCV in 46 (6.1%). These results suggested that these viruses, or other closely related viruses, are infecting horses in Brazil. However, none of the studied horses presented central nervous system infection symptoms. Conclusions Our results suggest that SLEV and ROCV previously circulated among horses in northeast, west-central and southeast Brazil. .


Assuntos
Animais , Anticorpos Antivirais/sangue , Vírus da Encefalite de St. Louis/imunologia , Encefalite de St. Louis/veterinária , Infecções por Flavivirus/veterinária , Doenças dos Cavalos/epidemiologia , Brasil/epidemiologia , Ensaio de Imunoadsorção Enzimática , Encefalite de St. Louis/diagnóstico , Encefalite de St. Louis/epidemiologia , Infecções por Flavivirus/diagnóstico , Infecções por Flavivirus/epidemiologia , Cavalos , Doenças dos Cavalos/diagnóstico , Estudos Soroepidemiológicos
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