Antibiotic profiling of multidrug resistant pathogens in one-day-old chicks imported from Belgium to benin.

BACKGROUND: Little data exist on the presence of resistant pathogens in day-old chicks imported into Benin. The occurrence of pathogenic bacteria was assessed in 180 one-day-old chicks imported from Belgium and received at the Cardinal Bernardin Gantin International Airport in Cotonou (Benin). The samples included swabbing the blisters of 180 chicks, followed by 18 pools of 10 swabs for bacterial isolation. Classic bacteriological methods based on Gram staining, culture on specific media and biochemical characterization were used. Antibacterial susceptibility screening to antibiotics was conducted using the Kirby-Bauer disc diffusion method, and the results were interpreted according to guidelines from the European Committee on Antimicrobial Susceptibility Testing (EUCAST). DNA extraction was performed by the heat treatment method. Resistance genes were screened by real-time PCR. RESULTS: We isolated 32 bacteria, including Escherichia coli (50%), Enterococcus spp. (28%), and coagulase-negative staphylococci (10%). The isolates were investigated for antibiotic resistance against antibiotics using the disk diffusion method and showed that in the Escherichia coli strains isolated, the highest rate of resistance was obtained against ciprofloxacin (81%), followed by trimethoprim + sulfamethoxazole (62%). Enterobacter cloacae was sensitive to all the antibiotics tested. Pseudomonas spp. resistant to amoxicillin and trimethoprim + sulfamethoxazole was noted. The SulII gene was found in all cloacal samples, while the SulI and blaTEM genes were present at 44.44% and 16.67%, respectively. CONCLUSION: This study confirms that imported day-old chicks can be a potential source of dissemination of resistant bacteria in poultry production. A system for immediate detection of resistant bacteria in chicks upon arrival in the country is thus needed.

Prevalence of cattle trypanosomosis in slaughterhouses and farms in Benin and impact on hematocrit in cattle.

OBJECTIVE: The present study aimed to determine the prevalence of bovine trypanosomosis in Benin. MATERIALS AND METHODS: For this purpose, the blood samples were taken from 932 cattle: 573 from slaughterhouses and 359 from the farms of the targeted divisions. After the blood samples, thick drops and blood smears were made. The capillary tubes filled with blood made it possible to determine the hematocrit in cattle. RESULTS: The prevalence of trypanosomosis in farms (27.02%) was significantly higher (p < 0.001) than that of animals in slaughterhouses (16.75%). In farms, the prevalence was 38.55% during the rainy season against 16.67% in the dry season (p < 0.001). In slaughterhouses, prevalences were 10.99%, 17.58%, and 21.50%, respectively, in Bohicon, Cotonou/Porto-Novo, and Parakou. Hematocrit in slaughterhouses was 24.17% and 31.44%, respectively, in infested and non-infested animals. In farms, this rate was 22.85% in infested animals and 29.31% in non-infested animals (p < 0.05). Young cattle are more vulnerable to trypanosomosis than older cattle. CONCLUSION: Given the endemic situation of bovine trypanosomosis and its impact on the economy, this knowledge of the health status of cattle will help out to seek ways and alternatives to reduce the damage.