RESUMO
A new plasmid, pGA1, has been isolated from Corynebacterium glutamicum LP-6, and its detailed restriction map has been prepared. The 4.9-kb plasmid has a G + C content of 57%. It replicates in C. glutamicum ATCC13032 and is compatible with the three other plasmids, pCC1, pBL1 and pHM1519, commonly used for vector construction for amino acid-producing corynebacteria. Fusions of pGA1 with different Escherichia coli replicons (transferred from E. coli to Corynebacterium via transformation of spheroplasts or by filter mating experiments with intact cells) are shown to be suitable as shuttle plasmids; some of them are highly stable in C. glutamicum, even when propagated without any selection pressure.
Assuntos
Corynebacterium/genética , Vetores Genéticos/genética , Plasmídeos/genética , Composição de Bases , Elementos de DNA Transponíveis/genética , Escherichia coli/genética , Mapeamento por Restrição , Esferoplastos/genética , Transformação BacterianaRESUMO
Eighteen related strains of Arthrobacter, Brevibacterium and Corynebacterium were used as indicator strains in an attempt to isolate corynephages from a large number of soils and from waste-water samples. Although no phages capable of producing plaques were isolated, one of the indicator strains used, Brevibacterium flavum ATCC 14067, was lysogenic for the inducible phage phi GA1. This phage was not observed to form plaques on any of the strains tested. phi GA1 is of B1-morphotype with a linear double-stranded DNA genome of 48.1 kb with cohesive ends; a restriction map is presented.
Assuntos
Bacteriófagos/genética , Brevibacterium/genética , DNA Viral/análise , Ativação Viral , Arthrobacter/genética , Bacteriófagos/crescimento & desenvolvimento , Corynebacterium/genética , Europa (Continente) , Japão , Lisogenia , Mapeamento por Restrição , Microbiologia do Solo , Microbiologia da ÁguaRESUMO
The host range of the virulent bacteriophage Cog among several strains of amino acid-producing corynebacteria is limited to Corynebacterium glutamicum LP-6. Cog is a typical corynephage of the Siphoviridae family (B1 morphotype) with an isometric head of 52 nm and a medium length, striated tail of 190 nm. It has a linear genome of 39.7 kb with cohesive ends and 53% G + C; a restriction map is presented, including regions of DNA homology (by hybridization) with the inducible phage particle phi GA1 from Brevibacterium flavum.