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1.
BMC Genomics ; 19(1): 719, 2018 Oct 01.
Artigo em Inglês | MEDLINE | ID: mdl-30285610

RESUMO

BACKGROUND: High-throughput proteomics was used to determine the role of the fish liver in defense responses to bacterial infection. This was done using a rainbow trout (Oncorhynchus mykiss) model following infection with Aeromonas salmonicida, the causative agent of furunculosis. The vertebrate liver has multifaceted functions in innate immunity, metabolism, and growth; we hypothesize this tissue serves a dual role in supporting host defense in parallel to metabolic adjustments that promote effective immune function. While past studies have reported mRNA responses to A. salmonicida in salmonids, the impact of bacterial infection on the liver proteome remains uncharacterized in fish. RESULTS: Rainbow trout were injected with A. salmonicida or PBS (control) and liver extracted 48 h later for analysis on a hybrid quadrupole-Orbitrap mass spectrometer. A label-free method was used for protein abundance profiling, which revealed a strong innate immune response along with evidence to support parallel rewiring of metabolic and growth systems. 3076 proteins were initially identified against all proteins (n = 71,293 RefSeq proteins) annotated in a single high-quality rainbow trout reference genome, of which 2433 were maintained for analysis post-quality filtering. Among the 2433 proteins, 109 showed significant differential abundance following A. salmonicida challenge, including many upregulated complement system and acute phase response proteins, in addition to molecules with putative functions that may support metabolic re-adjustments. We also identified novel expansions in the complement system due to gene and whole genome duplication events in salmonid evolutionary history, including eight C3 proteins showing differential changes in abundance. CONCLUSIONS: This study provides the first high-throughput proteomic examination of the fish liver in response to bacterial challenge, revealing novel markers for the host defense response, and evidence of metabolic remodeling in conjunction with activation of innate immunity.


Assuntos
Aeromonas salmonicida/fisiologia , Proteínas de Peixes/metabolismo , Fígado/metabolismo , Fígado/microbiologia , Oncorhynchus mykiss/metabolismo , Oncorhynchus mykiss/microbiologia , Proteômica , Animais , Ontologia Genética , Fígado/imunologia , Oncorhynchus mykiss/genética , Oncorhynchus mykiss/imunologia , Mapeamento de Interação de Proteínas
2.
Methods ; 57(2): 196-202, 2012 Jun.
Artigo em Inglês | MEDLINE | ID: mdl-22465796

RESUMO

Chromatin is dynamically regulated, and proteomic analysis of its composition can provide important information about chromatin functional components. Many DNA replication proteins for example bind chromatin at specific times during the cell cycle. Proteomic investigation can also be used to characterize changes in chromatin composition in response to perturbations such as DNA damage, while useful information is obtained by testing the effects on chromatin composition of mutations in chromosome stability pathways. We have successfully used the method of stable isotope labeling by amino acids in cell culture (SILAC) for quantitative proteomic analysis of normal and pathological changes to yeast chromatin. Here we describe this proteomic method for analyzing changes to Saccharomyces cerevisiae chromatin, illustrating the procedure with an analysis of the changes that occur in chromatin composition as cells progress from a G1 phase block (induced by alpha factor) into S phase (in the presence of DNA replication inhibitor hydroxyurea).


Assuntos
Replicação do DNA , Proteínas de Ligação a DNA/metabolismo , Proteínas de Saccharomyces cerevisiae/metabolismo , Aminoácidos/química , Aminoácidos/metabolismo , Núcleo Celular/genética , Núcleo Celular/metabolismo , Cromatina/isolamento & purificação , Cromatina/metabolismo , Proteínas de Ligação a DNA/química , Proteínas de Ligação a DNA/isolamento & purificação , Hidroxiureia/farmacologia , Marcação por Isótopo , Inibidores da Síntese de Ácido Nucleico/farmacologia , Proteoma/metabolismo , Proteômica , Saccharomyces cerevisiae/efeitos dos fármacos , Saccharomyces cerevisiae/genética , Saccharomyces cerevisiae/metabolismo , Proteínas de Saccharomyces cerevisiae/química , Proteínas de Saccharomyces cerevisiae/isolamento & purificação , Esferoplastos/efeitos dos fármacos , Esferoplastos/genética , Esferoplastos/metabolismo , Espectrometria de Massas em Tandem
3.
Proteomics ; 12(22): 3403-6, 2012 Nov.
Artigo em Inglês | MEDLINE | ID: mdl-23001906

RESUMO

We report the first survey of the dromedary camel urinary proteome. Proteins retained from ultrafiltration of urine were analysed by GeLC-MS/MS (SDS-PAGE followed by LC-MS/MS). In the absence of a complete camel genome sequence, the number of protein identifications was maximised by searching three primary sequence databases: Swiss-Prot, alpaca and camel EST. This search strategy enabled the identification of 1274 peptide sequences, of which 735 were found in at least two independent samples. Functional annotations for proteins identified from alpaca and camel EST sequences were mapped from basic local alignment search tool (protein) searches. These 735 peptides, which included many novel sequences found only in the camel EST database, were grouped to 147 protein descriptors. Gene ontology term analysis of human proteins with sequence similarity showed that camel urine may be particularly enriched in proteins from extracellular compartments and vesicles, and with functions that include carbohydrate-binding and peptidase inhibitor activities. If their biological functions are conserved between species, many of the camel urinary proteins could be involved in various stress and immune responses, and some may have antimicrobial activities.


Assuntos
Camelus/urina , Mapeamento de Peptídeos/métodos , Proteinúria/urina , Proteinúria/veterinária , Proteoma/análise , Proteômica/métodos , Animais , Cromatografia Líquida , Bases de Dados de Proteínas , Eletroforese em Gel de Poliacrilamida , Feminino , Espectrometria de Massas em Tandem , Urina/química
4.
Mol Microbiol ; 79(6): 1574-93, 2011 Mar.
Artigo em Inglês | MEDLINE | ID: mdl-21269335

RESUMO

Post-translational modifications of proteins play key roles in eukaryotic growth, differentiation and environmental adaptation. In model systems the ubiquitination of specific proteins contributes to the control of cell cycle progression, stress adaptation and metabolic reprogramming. We have combined molecular, cellular and proteomic approaches to examine the roles of ubiquitination in Candida albicans, because little is known about ubiquitination in this major fungal pathogen of humans. Independent null (ubi4/ubi4) and conditional (MET3p-UBI4/ubi4) mutations were constructed at the C. albicans polyubiquitin-encoding locus. These mutants displayed morphological and cell cycle defects, as well as sensitivity to thermal, oxidative and cell wall stresses. Furthermore, ubi4/ubi4 cells rapidly lost viability under starvation conditions. Consistent with these phenotypes, proteins with roles in stress responses (Gnd1, Pst2, Ssb1), metabolism (Acs2, Eno1, Fba1, Gpd2, Pdx3, Pgk1, Tkl1) and ubiquitination (Ubi4, Ubi3, Pre1, Pre3, Rpt5) were among the ubiquitination targets we identified, further indicating that ubiquitination plays key roles in growth, stress responses and metabolic adaptation in C. albicans. Clearly ubiquitination plays key roles in the regulation of fundamental cellular processes that underpin the pathogenicity of this medically important fungus. This was confirmed by the observation that the virulence of C. albicans ubi4/ubi4 cells is significantly attenuated.


Assuntos
Candida albicans/fisiologia , Candida albicans/patogenicidade , Candidíase/microbiologia , Proteínas Fúngicas/metabolismo , Regulação Fúngica da Expressão Gênica , Proteômica , Animais , Candida albicans/química , Candida albicans/crescimento & desenvolvimento , Feminino , Proteínas Fúngicas/química , Proteínas Fúngicas/genética , Humanos , Camundongos , Camundongos Endogâmicos BALB C , Dados de Sequência Molecular , Estresse Fisiológico , Ubiquitinação , Virulência
5.
Front Immunol ; 13: 873390, 2022.
Artigo em Inglês | MEDLINE | ID: mdl-35734164

RESUMO

Many animals of scientific importance lack species-specific reagents (e.g., monoclonal antibodies) for in-depth studies of immune proteins. Mass spectrometry (MS)-based proteomics has emerged as a useful method for monitoring changes in protein abundance and modifications in non-model species. It can be used to quantify hundreds of candidate immune molecules simultaneously without the generation of new reagents. Here, we used MS-based proteomics to identify and quantify candidate immune proteins in the plasma of the nurse shark (Ginglymostoma cirratum), a cartilaginous fish and representative of the most basal extant vertebrate lineage with an immunoglobulin-based immune system. Mass spectrometry-based LC-MS/MS was performed on the blood plasma of nurse sharks immunized with human serum albumin (n=4) or sham immunized (n=1), and sampled at days 0 (baseline control), 1, 2, 3, 5, 7, 14, 21, 28, 25, 42 and 49. An antigen-specific antibody response was experimentally confirmed post-immunization. To provide a high-quality reference to identify proteins, we assembled and annotated a multi-tissue de novo transcriptome integrating long- and short-read sequence data. This comprised 62,682 contigs containing open reading frames (ORFs) with a length >80 amino acids. Using this transcriptome, we reliably identified 626 plasma proteins which were broadly categorized into coagulation, immune, and metabolic functional groups. To assess the feasibility of performing LC-MS/MS proteomics in nurse shark in the absence of species-specific protein annotations, we compared the results to an alternative strategy, mapping peptides to proteins predicted in the genome assembly of a related species, the whale shark (Rhincodon typus). This approach reliably identified 297 proteins, indicating that useful data on the plasma proteome may be obtained in many instances despite the absence of a species-specific reference protein database. Among the plasma proteins defined against the nurse shark transcriptome, fifteen showed consistent changes in abundance across the immunized shark individuals, indicating a role in the immune response. These included alpha-2-macroglobulin (A2M) and a novel protein yet to be characterized in diverse vertebrate lineages. Overall, this study enhances genetic and protein-level resources for nurse shark research and vastly improves our understanding of the elasmobranch plasma proteome, including its remodelling following immune stimulation.


Assuntos
Proteoma , Tubarões , Animais , Cromatografia Líquida , Plasma , Proteoma/metabolismo , Tubarões/genética , Espectrometria de Massas em Tandem
6.
Proteomics ; 10(2): 212-23, 2010 Jan.
Artigo em Inglês | MEDLINE | ID: mdl-19941307

RESUMO

Candida glabrata is a major fungal pathogen of humans, and the virulence of C. glabrata is increased by inactivation of the transcription factor, Ace2. Our previous examination of the effects of Ace2 inactivation upon the intracellular proteome suggested that the hypervirulence of C. glabrata ace2 mutants might be caused by differences in the secretome. Therefore in this study we have characterised the C. glabrata secretome and examined the effects of Ace2 inactivation upon this extracellular proteome. We have identified 31 distinct proteins in the secretome of wild-type C. glabrata cells by MS/MS of proteins that were precipitated from the growth medium and enriched by affinity chromatography on concanavalin A. Most of these proteins are predicted to be cell wall proteins, cell wall modifying enzymes and aspartyl proteinases. The endochitinase Cts1 and the endoglucanase Egt2 were not detected in the C. glabrata secretome following Ace2 inactivation. This can account for the cell separation defect of C. glabrata ace2 cells. Ace2 inactivation also resulted in the detection of new proteins in the C. glabrata secretome. The release of such proteins might contribute to the hypervirulence of ace2 cells.


Assuntos
Candida glabrata/química , Proteínas Fúngicas/metabolismo , Proteoma/análise , Candida glabrata/efeitos dos fármacos , Candida glabrata/metabolismo , Candida glabrata/patogenicidade , Parede Celular/química , Doxiciclina/farmacologia , Espaço Extracelular/química , Proteínas Fúngicas/genética , Glicosilação , Sinais Direcionadores de Proteínas , Proteoma/química , Transcrição Gênica , Virulência
7.
Brief Bioinform ; 9(2): 174-88, 2008 Mar.
Artigo em Inglês | MEDLINE | ID: mdl-18281347

RESUMO

Proteomics, the study of the protein complement of a biological system, is generating increasing quantities of data from rapidly developing technologies employed in a variety of different experimental workflows. Experimental processes, e.g. for comparative 2D gel studies or LC-MS/MS analyses of complex protein mixtures, involve a number of steps: from experimental design, through wet and dry lab operations, to publication of data in repositories and finally to data annotation and maintenance. The presence of inaccuracies throughout the processing pipeline, however, results in data that can be untrustworthy, thus offsetting the benefits of high-throughput technology. While researchers and practitioners are generally aware of some of the information quality issues associated with public proteomics data, there are few accepted criteria and guidelines for dealing with them. In this article, we highlight factors that impact on the quality of experimental data and review current approaches to information quality management in proteomics. Data quality issues are considered throughout the lifecycle of a proteomics experiment, from experiment design and technique selection, through data analysis, to archiving and sharing.


Assuntos
Armazenamento e Recuperação da Informação , Proteômica , Controle de Qualidade , Sistemas de Gerenciamento de Base de Dados , Eletroforese em Gel Bidimensional , Armazenamento e Recuperação da Informação/métodos , Armazenamento e Recuperação da Informação/normas , Espectrometria de Massas , Proteínas/análise , Proteômica/instrumentação , Proteômica/métodos , Proteômica/normas , Software
8.
Front Immunol ; 11: 581070, 2020.
Artigo em Inglês | MEDLINE | ID: mdl-33133099

RESUMO

Vaccination plays a critical role in the protection of humans and other animals from infectious diseases. However, the same vaccine often confers different protection levels among individuals due to variation in genetics and/or immunological histories. While this represents a well-recognized issue in humans, it has received little attention in fish. Here we address this knowledge gap in a proteomic study of rainbow trout (Oncorhynchus mykiss, Walbaum), using non-lethal repeated blood sampling to establish the plasma protein response of individual fish following immunization. Six trout were immunized with adjuvanted hen egg-white lysozyme (HEL) and peripheral blood sampled at ten time points from day 0 to day 84 post-injection. We confirm that an antigen-specific antibody response to HEL was raised, showing differences in timing and magnitude among individuals. Using label-free liquid chromatography-mass spectrometry, we quantified the abundance of 278 plasma proteins across the timecourse. As part of the analysis, we show that this approach can distinguish many (but not all) duplicated plasma proteins encoded by paralogous genes retained from the salmonid-specific whole genome duplication event. Global variation in the plasma proteome was predominantly explained by individual differences among fish. However, sampling day explained a major component of variation in abundance for a statistically defined subset of 41 proteins, representing 15% of those detected. These proteins clustered into five groups showing distinct temporal responses to HEL immunization at the population level, and include classical immune (e.g. complement system members) and acute phase molecules (e.g. apolipoproteins, haptoglobins), several enzymes and other proteins supporting the immune response, in addition to evolutionarily conserved molecules that are as yet uncharacterized. Overall, this study improves our understanding of the fish plasma proteome, provides valuable marker proteins for different phases of the immune response, and has implications for vaccine development and the design of immune challenge experiments.


Assuntos
Proteínas de Peixes/sangue , Proteínas de Peixes/imunologia , Oncorhynchus mykiss/sangue , Oncorhynchus mykiss/imunologia , Proteoma/imunologia , Adjuvantes Imunológicos/administração & dosagem , Animais , Proteínas Aviárias/administração & dosagem , Proteínas Aviárias/imunologia , Proteínas Sanguíneas/classificação , Proteínas Sanguíneas/imunologia , Proteínas do Ovo/administração & dosagem , Proteínas do Ovo/imunologia , Feminino , Proteínas de Peixes/classificação , Imunização/veterinária , Masculino , Muramidase/administração & dosagem , Muramidase/imunologia , Filogenia , Proteômica
9.
Mol Ecol ; 18(3): 415-29, 2009 Feb.
Artigo em Inglês | MEDLINE | ID: mdl-19161465

RESUMO

Population genetics of the amphibian pathogen Batrachochytrium dendrobatidis (Bd) show that isolates are highly related and globally homogenous, data that are consistent with the recent epidemic spread of a previously endemic organism. Highly related isolates are predicted to be functionally similar due to low levels of heritable genetic diversity. To test this hypothesis, we took a global panel of Bd isolates and measured (i) the genetic relatedness among isolates, (ii) proteomic profiles of isolates, (iii) the susceptibility of isolates to the antifungal drug caspofungin, (iv) the variation among isolates in growth and phenotypic characteristics, and (v) the virulence of isolates against the European common toad Bufo bufo. Our results show (i) genotypic differentiation among isolates, (ii) proteomic differentiation among isolates, (iii) no significant differences in susceptibility to caspofungin, (iv) differentiation in growth and phenotypic/morphological characters, and (v) differential virulence in B. bufo. Specifically, our data show that Bd isolates can be profiled by their genotypic and proteomic characteristics, as well as by the size of their sporangia. Bd genotypic and phenotypic distance matrices are significantly correlated, showing that less-related isolates are more biologically unique. Mass spectrometry has identified a set of candidate genes associated with inter-isolate variation. Our data show that, despite its rapid global emergence, Bd isolates are not identical and differ in several important characters that are linked to virulence. We argue that future studies need to clarify the mechanism(s) and rate at which Bd is evolving, and the impact that such variation has on the host-pathogen dynamic.


Assuntos
Anuros/microbiologia , Quitridiomicetos/genética , Quitridiomicetos/patogenicidade , Perfilação da Expressão Gênica , Técnicas de Tipagem Micológica , Fenótipo , Proteoma , Animais , Antifúngicos/farmacologia , Bufo bufo/microbiologia , Caspofungina , Quitridiomicetos/classificação , Quitridiomicetos/efeitos dos fármacos , Equinocandinas/farmacologia , Proteínas Fúngicas/genética , Proteínas Fúngicas/metabolismo , Genótipo , Lipopeptídeos , Testes de Sensibilidade Microbiana , Virulência/genética
10.
J Proteomics ; 192: 114-124, 2019 02 10.
Artigo em Inglês | MEDLINE | ID: mdl-30153513

RESUMO

In fish used for food production and scientific research, fast growth can be achieved via selective breeding or induced instantaneously via growth hormone (GH) transgenesis (GHT). The proteomic basis for these distinct routes towards a similar higher phenotype remains uncharacterized, as are associated implications for health parameters. We addressed this knowledge gap using skeletal muscle proteomics in coho salmon (Oncorhynchus kisutch), hypothesising that i) selective breeding and GHT are underpinned by both parallel and unique changes in growth systems, and ii) rapidly-growing fish strains have lowered scope to allocate resources towards immune function. Quantitative profiling of GHT and growth-selected strains was done in comparison to wild-type after injection with PBS (control) or Poly I:C (to mimic infection). We identified remodelling of the muscle proteome in each growth-enhanced strain that was strikingly non-overlapping. GHT was characterized by focal upregulation of systems driving protein synthesis, while the growth-selected fish presented a larger and more diverse set of changes, consistent with complex alterations to many metabolic and cellular pathways. Poly I:C had little detectable effect on the muscle proteome. This study demonstrates that distinct proteome profiles can explain outwardly similar enhanced growth phenotypes, improving our understanding of growth mechanisms in anthropogenic animal strains. SIGNIFICANCE: This work provides the first proteomic insights into mechanisms underpinning different anthropogenic routes to rapid growth in salmon. High-throughput proteomic profiling was used to reveal changes supporting enhanced growth, comparing skeletal muscle of growth hormone transgenic (GHT) and selectively-bred salmon strains with their wild-type counterparts. Contrasting past mRNA-level comparisons of the same fish strains, our data reveals a surprisingly substantial proteomic divergence between the GHT and selectively bred strains. The findings demonstrate that many unique molecular mechanisms underlie growth-enhanced phenotypes in different types of fish strain used for food production and scientific research.


Assuntos
Animais Geneticamente Modificados , Proteínas de Peixes , Hormônio do Crescimento , Oncorhynchus kisutch , Proteômica , Seleção Artificial , Animais , Animais Geneticamente Modificados/genética , Animais Geneticamente Modificados/metabolismo , Proteínas de Peixes/genética , Proteínas de Peixes/metabolismo , Hormônio do Crescimento/genética , Hormônio do Crescimento/metabolismo , Oncorhynchus kisutch/genética , Oncorhynchus kisutch/metabolismo
11.
Nat Biotechnol ; 21(3): 247-54, 2003 Mar.
Artigo em Inglês | MEDLINE | ID: mdl-12610571

RESUMO

Both the generation and the analysis of proteome data are becoming increasingly widespread, and the field of proteomics is moving incrementally toward high-throughput approaches. Techniques are also increasing in complexity as the relevant technologies evolve. A standard representation of both the methods used and the data generated in proteomics experiments, analogous to that of the MIAME (minimum information about a microarray experiment) guidelines for transcriptomics, and the associated MAGE (microarray gene expression) object model and XML (extensible markup language) implementation, has yet to emerge. This hinders the handling, exchange, and dissemination of proteomics data. Here, we present a UML (unified modeling language) approach to proteomics experimental data, describe XML and SQL (structured query language) implementations of that model, and discuss capture, storage, and dissemination strategies. These make explicit what data might be most usefully captured about proteomics experiments and provide complementary routes toward the implementation of a proteome repository.


Assuntos
Sistemas de Gerenciamento de Base de Dados , Bases de Dados de Proteínas , Armazenamento e Recuperação da Informação/métodos , Proteínas/química , Proteômica/métodos , Documentação/métodos , Hipermídia , Disseminação de Informação/métodos , Modelos Moleculares , Conformação Proteica , Proteínas/genética , Proteínas/metabolismo , Análise de Sequência de Proteína/métodos , Software , Design de Software , Interface Usuário-Computador
12.
Int J Antimicrob Agents ; 48(5): 521-527, 2016 Nov.
Artigo em Inglês | MEDLINE | ID: mdl-27665523

RESUMO

The Burkholderia cepacia complex (Bcc) is notorious for the life-threatening pulmonary infections it causes in patients with cystic fibrosis. The multidrug-resistant nature of Bcc and differing infective Bcc species make the design of appropriate treatment regimens challenging. Previous synergy studies have failed to take account of the species of Bcc isolates. Etest methodology was used to facilitate minimum inhibitory concentration (MIC) and antimicrobial combination testing on 258 isolates of Bcc, identified to species level by matrix-assisted laser desorption/ionisation time-of-flight mass spectrometry (MALDI-TOF/MS). The most active antimicrobials were trimethoprim/sulphamethoxazole, doxycycline and minocycline (52.5%, 46.4% and 45.9% of isolates susceptible, respectively). Synergy was observed in 9.2% of the 1799 combinations tested; the most common synergistic combinations were tobramycin + ceftazidime, meropenem + tobramycin and levofloxacin + piperacillin/tazobactam (35.4%, 32.3% and 22.2% synergy, respectively). Antimicrobial susceptibility analysis revealed differences between Burkholderia cenocepacia and Burkholderia multivorans. Disparity in clinical outcome during infection with these two micro-organisms necessitates further investigation into the clinical outcomes of treatment regimens in light of species identification and in vitro antimicrobial susceptibility studies.


Assuntos
Antibacterianos/farmacologia , Complexo Burkholderia cepacia/efeitos dos fármacos , Interações Medicamentosas , Testes de Sensibilidade Microbiana/métodos , Complexo Burkholderia cepacia/química , Complexo Burkholderia cepacia/classificação , Complexo Burkholderia cepacia/isolamento & purificação , Feminino , Humanos , Masculino , Estudos Retrospectivos , Espectrometria de Massas por Ionização e Dessorção a Laser Assistida por Matriz , Adulto Jovem
13.
Mol Biol Cell ; 22(5): 687-702, 2011 Mar 01.
Artigo em Inglês | MEDLINE | ID: mdl-21209325

RESUMO

Posttranslational modifications of proteins play critical roles in the control of cellular differentiation, development, and environmental adaptation. In particular, the covalent attachment of the small ubiquitin-like modifier, SUMO, to target proteins (sumoylation) regulates cell cycle progression, transcription, nucleocytoplasmic transport, and stress responses. Here we combine proteomic, molecular, and cellular approaches to examine the roles of sumoylation in the major fungal pathogen of humans, Candida albicans. Using an N-terminally FLAG-tagged SUMO, 31 sumoylated proteins were identified in C. albicans with roles in stress responses (e.g., Hsp60, Hsp70 family members, Hsp104), the cytoskeleton and polarized growth (e.g., Tub1, Cct7, Mlc1), secretion, and endocytosis (e.g., Lsp1, Sec24, Sec7). The output from this proteomic screen was entirely consistent with the phenotypes of C. albicans mutants in which the single SUMO-encoding locus (SMT3) was inactivated or down-regulated. C. albicans smt3/smt3 cells displayed defects in growth, morphology, cell separation, nuclear segregation, and chitin deposition, suggesting important roles for sumoylation in cell cycle control. Smt3/smt3 cells also displayed sensitivity to thermal, oxidative, and cell wall stresses as well as to the antifungal drug caspofungin. Mutation of consensus sumoylation sites in Hsp60 and Hsp104 affected the resistance of C. albicans to thermal stress. Furthermore, signaling via the cell integrity pathway was defective in C. albicans smt3/smt3 cells. These observations provide mechanistic explanations for many of the observed phenotypic effects of Smt3 inactivation upon C. albicans growth and environmental adaptation. Clearly sumoylation plays key roles in fundamental cellular processes that underpin the pathogenicity of this medically important fungus.


Assuntos
Candida albicans/crescimento & desenvolvimento , Candida albicans/patogenicidade , Proteínas Fúngicas/metabolismo , Estresse Fisiológico , Sumoilação , Adaptação Fisiológica/efeitos dos fármacos , Candida albicans/citologia , Candida albicans/enzimologia , Ciclo Celular/efeitos dos fármacos , Cisteína/farmacologia , Ativação Enzimática/efeitos dos fármacos , Deleção de Genes , Genes Essenciais , Hifas/citologia , Hifas/efeitos dos fármacos , Hifas/metabolismo , Metionina/farmacologia , Viabilidade Microbiana/efeitos dos fármacos , Proteínas Quinases Ativadas por Mitógeno/metabolismo , Morfogênese/efeitos dos fármacos , Mutação/genética , Fenótipo , Proteômica , Estresse Fisiológico/efeitos dos fármacos , Sumoilação/efeitos dos fármacos
14.
PLoS One ; 6(10): e27076, 2011.
Artigo em Inglês | MEDLINE | ID: mdl-22073125

RESUMO

Inflammatory bowel disease (IBD) arises in genetically susceptible individuals as a result of an unidentified environmental trigger, possibly a hitherto unknown bacterial pathogen. Twenty-six clinical isolates of Sutterella wadsworthensis were obtained from 134 adults and 61 pediatric patients undergoing colonoscopy, of whom 69 and 29 respectively had IBD. S. wadsworthensis was initially more frequently isolated from IBD subjects, hence this comprehensive study was undertaken to elucidate its role in IBD. Utilizing these samples, a newly designed PCR was developed, to study the prevalence of this bacterium in adult patients with ulcerative colitis (UC). Sutterella wadsworthensis was detected in 83.8% of adult patients with UC as opposed to 86.1% of control subjects (p = 0.64). Selected strains from IBD cases and controls were studied to elicit morphological, proteomic, genotypic and pathogenic differences. This study reports Scanning Electron Microscopy (SEM) appearances and characteristic MALDI-TOF MS protein profiles of S. wadsworthensis for the very first time. SEM showed that the bacterium is pleomorphic, existing in predominantly two morphological forms, long rods and coccobacilli. No differences were noted in the MALDI-TOF mass spectrometry proteomic analysis. There was no distinct clustering of strains identified from cases and controls on sequence analysis. Cytokine response after monocyte challenge with strains from patients with IBD and controls did not yield any significant differences. Our studies indicate that S. wadsworthensis is unlikely to play a role in the pathogenesis of IBD. Strains from cases of IBD could not be distinguished from those identified from controls.


Assuntos
Colite Ulcerativa/microbiologia , Colo/microbiologia , Doença de Crohn/microbiologia , Bactérias Gram-Negativas/isolamento & purificação , Infecções por Bactérias Gram-Negativas/microbiologia , Mucosa Intestinal/microbiologia , Adulto , Estudos de Casos e Controles , Criança , Estudos de Coortes , Colite Ulcerativa/epidemiologia , Colite Ulcerativa/genética , Colonoscopia , Doença de Crohn/epidemiologia , Doença de Crohn/genética , DNA Bacteriano/genética , Feminino , Genótipo , Bactérias Gram-Negativas/genética , Infecções por Bactérias Gram-Negativas/epidemiologia , Infecções por Bactérias Gram-Negativas/genética , Humanos , Masculino , Pessoa de Meia-Idade , Fenótipo , Filogenia , Reação em Cadeia da Polimerase , Prevalência , Proteômica , Sensibilidade e Especificidade , Espectrometria de Massas por Ionização e Dessorção a Laser Assistida por Matriz , Reino Unido/epidemiologia
15.
Mol Cell Proteomics ; 5(7): 1205-11, 2006 Jul.
Artigo em Inglês | MEDLINE | ID: mdl-16567383

RESUMO

Increasing numbers of large proteomic datasets are becoming available. As attempts are made to interpret these datasets and integrate them with other forms of genomic data, researchers are becoming more aware of the importance of data quality with respect to protein identification. We present three simple and universal metrics that describe different aspects of the quality of protein identifications by peptide mass fingerprinting. Hit ratio gives an indication of the signal-to-noise ratio in a mass spectrum, mass coverage measures the amount of protein sequence matched, and excess of limit-digested peptides reflects the completeness of the digestion that precedes the peptide mass fingerprinting. Receiver-operating characteristic plots show that the novel metric, excess of limit-digested peptides, can discriminate between correct and random matches more accurately than search score when validating the results from a state-of-the-art protein identification software system (Mascot) especially when combined with the two other metrics, hit ratio and mass coverage. Recommendations are made regarding the use of the metrics when reporting protein identification experiments.


Assuntos
Espectrometria de Massas/normas , Proteômica/métodos , Controle de Qualidade , Sistema Métrico , Mapeamento de Peptídeos , Proteínas/análise , Proteínas/química , Curva ROC
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